Amino acid dipepetide frequency for Microbacterium aurum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.353AlaAla: 23.353 ± 0.275	0.743AlaCys: 0.743 ± 0.031	9.358AlaAsp: 9.358 ± 0.122	8.223AlaGlu: 8.223 ± 0.091	3.905AlaPhe: 3.905 ± 0.066	12.793AlaGly: 12.793 ± 0.138	2.775AlaHis: 2.775 ± 0.055	5.931AlaIle: 5.931 ± 0.074	2.659AlaLys: 2.659 ± 0.07	14.554AlaLeu: 14.554 ± 0.174	2.706AlaMet: 2.706 ± 0.052	2.125AlaAsn: 2.125 ± 0.051	7.005AlaPro: 7.005 ± 0.11	4.252AlaGln: 4.252 ± 0.069	10.105AlaArg: 10.105 ± 0.141	7.302AlaSer: 7.302 ± 0.092	8.009AlaThr: 8.009 ± 0.112	12.258AlaVal: 12.258 ± 0.125	2.08AlaTrp: 2.08 ± 0.055	2.457AlaTyr: 2.457 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.77CysAla: 0.77 ± 0.03	0.06CysCys: 0.06 ± 0.009	0.338CysAsp: 0.338 ± 0.02	0.273CysGlu: 0.273 ± 0.018	0.18CysPhe: 0.18 ± 0.014	0.595CysGly: 0.595 ± 0.03	0.117CysHis: 0.117 ± 0.011	0.187CysIle: 0.187 ± 0.014	0.067CysLys: 0.067 ± 0.01	0.453CysLeu: 0.453 ± 0.023	0.088CysMet: 0.088 ± 0.01	0.092CysAsn: 0.092 ± 0.01	0.305CysPro: 0.305 ± 0.022	0.134CysGln: 0.134 ± 0.013	0.377CysArg: 0.377 ± 0.021	0.307CysSer: 0.307 ± 0.019	0.337CysThr: 0.337 ± 0.021	0.417CysVal: 0.417 ± 0.018	0.067CysTrp: 0.067 ± 0.008	0.104CysTyr: 0.104 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	10.538AspAla: 10.538 ± 0.11	0.253AspCys: 0.253 ± 0.016	4.384AspAsp: 4.384 ± 0.085	4.414AspGlu: 4.414 ± 0.064	1.633AspPhe: 1.633 ± 0.042	6.16AspGly: 6.16 ± 0.099	1.168AspHis: 1.168 ± 0.038	2.529AspIle: 2.529 ± 0.045	0.993AspLys: 0.993 ± 0.036	6.404AspLeu: 6.404 ± 0.07	0.832AspMet: 0.832 ± 0.032	0.907AspAsn: 0.907 ± 0.032	4.21AspPro: 4.21 ± 0.066	1.592AspGln: 1.592 ± 0.04	4.748AspArg: 4.748 ± 0.08	2.25AspSer: 2.25 ± 0.051	3.035AspThr: 3.035 ± 0.065	5.662AspVal: 5.662 ± 0.087	0.907AspTrp: 0.907 ± 0.03	1.276AspTyr: 1.276 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	6.951GluAla: 6.951 ± 0.094	0.271GluCys: 0.271 ± 0.02	2.655GluAsp: 2.655 ± 0.052	2.771GluGlu: 2.771 ± 0.069	1.682GluPhe: 1.682 ± 0.042	3.863GluGly: 3.863 ± 0.071	1.391GluHis: 1.391 ± 0.043	2.879GluIle: 2.879 ± 0.06	1.359GluLys: 1.359 ± 0.054	6.311GluLeu: 6.311 ± 0.091	0.849GluMet: 0.849 ± 0.027	1.151GluAsn: 1.151 ± 0.034	2.9GluPro: 2.9 ± 0.07	2.145GluGln: 2.145 ± 0.056	5.29GluArg: 5.29 ± 0.095	2.584GluSer: 2.584 ± 0.059	2.99GluThr: 2.99 ± 0.05	4.694GluVal: 4.694 ± 0.07	0.825GluTrp: 0.825 ± 0.032	1.213GluTyr: 1.213 ± 0.039	0.0GluXaa: 0.0 ± 0.0
Phe	4.402PheAla: 4.402 ± 0.077	0.152PheCys: 0.152 ± 0.014	2.426PheAsp: 2.426 ± 0.054	1.665PheGlu: 1.665 ± 0.046	0.993PhePhe: 0.993 ± 0.037	3.168PheGly: 3.168 ± 0.064	0.604PheHis: 0.604 ± 0.026	1.084PheIle: 1.084 ± 0.038	0.418PheLys: 0.418 ± 0.02	2.723PheLeu: 2.723 ± 0.062	0.401PheMet: 0.401 ± 0.018	0.628PheAsn: 0.628 ± 0.029	1.364PhePro: 1.364 ± 0.041	0.706PheGln: 0.706 ± 0.028	1.804PheArg: 1.804 ± 0.044	1.594PheSer: 1.594 ± 0.041	2.029PheThr: 2.029 ± 0.049	2.759PheVal: 2.759 ± 0.057	0.451PheTrp: 0.451 ± 0.023	0.649PheTyr: 0.649 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	11.223GlyAla: 11.223 ± 0.137	0.56GlyCys: 0.56 ± 0.029	5.19GlyAsp: 5.19 ± 0.077	4.924GlyGlu: 4.924 ± 0.077	3.118GlyPhe: 3.118 ± 0.059	7.429GlyGly: 7.429 ± 0.116	1.846GlyHis: 1.846 ± 0.052	4.869GlyIle: 4.869 ± 0.079	2.095GlyLys: 2.095 ± 0.061	8.217GlyLeu: 8.217 ± 0.095	1.897GlyMet: 1.897 ± 0.044	1.584GlyAsn: 1.584 ± 0.04	3.746GlyPro: 3.746 ± 0.065	2.511GlyGln: 2.511 ± 0.056	6.492GlyArg: 6.492 ± 0.078	4.841GlySer: 4.841 ± 0.074	5.643GlyThr: 5.643 ± 0.088	7.767GlyVal: 7.767 ± 0.097	1.627GlyTrp: 1.627 ± 0.05	2.104GlyTyr: 2.104 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	2.647HisAla: 2.647 ± 0.052	0.114HisCys: 0.114 ± 0.012	1.343HisAsp: 1.343 ± 0.043	1.149HisGlu: 1.149 ± 0.034	0.544HisPhe: 0.544 ± 0.023	1.917HisGly: 1.917 ± 0.042	0.612HisHis: 0.612 ± 0.03	0.779HisIle: 0.779 ± 0.029	0.294HisLys: 0.294 ± 0.016	2.147HisLeu: 2.147 ± 0.052	0.32HisMet: 0.32 ± 0.02	0.323HisAsn: 0.323 ± 0.018	1.608HisPro: 1.608 ± 0.047	0.505HisGln: 0.505 ± 0.023	1.681HisArg: 1.681 ± 0.046	0.901HisSer: 0.901 ± 0.031	1.036HisThr: 1.036 ± 0.033	1.709HisVal: 1.709 ± 0.045	0.28HisTrp: 0.28 ± 0.017	0.439HisTyr: 0.439 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.162IleAla: 7.162 ± 0.094	0.219IleCys: 0.219 ± 0.017	3.643IleAsp: 3.643 ± 0.057	2.695IleGlu: 2.695 ± 0.059	1.06IlePhe: 1.06 ± 0.037	4.469IleGly: 4.469 ± 0.082	0.756IleHis: 0.756 ± 0.03	1.672IleIle: 1.672 ± 0.046	0.756IleLys: 0.756 ± 0.035	3.772IleLeu: 3.772 ± 0.063	0.596IleMet: 0.596 ± 0.024	0.838IleAsn: 0.838 ± 0.028	2.395IlePro: 2.395 ± 0.048	0.979IleGln: 0.979 ± 0.033	2.884IleArg: 2.884 ± 0.058	2.132IleSer: 2.132 ± 0.052	2.75IleThr: 2.75 ± 0.061	4.634IleVal: 4.634 ± 0.073	0.519IleTrp: 0.519 ± 0.023	0.727IleTyr: 0.727 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	2.409LysAla: 2.409 ± 0.063	0.07LysCys: 0.07 ± 0.009	1.104LysAsp: 1.104 ± 0.04	0.905LysGlu: 0.905 ± 0.036	0.41LysPhe: 0.41 ± 0.02	1.51LysGly: 1.51 ± 0.047	0.418LysHis: 0.418 ± 0.023	0.889LysIle: 0.889 ± 0.035	0.772LysLys: 0.772 ± 0.04	1.835LysLeu: 1.835 ± 0.048	0.369LysMet: 0.369 ± 0.018	0.503LysAsn: 0.503 ± 0.021	1.089LysPro: 1.089 ± 0.031	0.683LysGln: 0.683 ± 0.029	1.547LysArg: 1.547 ± 0.048	1.035LysSer: 1.035 ± 0.034	1.359LysThr: 1.359 ± 0.043	1.651LysVal: 1.651 ± 0.053	0.24LysTrp: 0.24 ± 0.015	0.432LysTyr: 0.432 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	14.945LeuAla: 14.945 ± 0.164	0.539LeuCys: 0.539 ± 0.025	6.587LeuAsp: 6.587 ± 0.089	5.035LeuGlu: 5.035 ± 0.083	2.8LeuPhe: 2.8 ± 0.056	8.715LeuGly: 8.715 ± 0.109	1.872LeuHis: 1.872 ± 0.043	4.321LeuIle: 4.321 ± 0.079	1.681LeuLys: 1.681 ± 0.046	9.822LeuLeu: 9.822 ± 0.149	1.581LeuMet: 1.581 ± 0.042	1.678LeuAsn: 1.678 ± 0.047	5.457LeuPro: 5.457 ± 0.08	2.764LeuGln: 2.764 ± 0.057	7.904LeuArg: 7.904 ± 0.102	5.536LeuSer: 5.536 ± 0.081	6.544LeuThr: 6.544 ± 0.079	9.171LeuVal: 9.171 ± 0.124	1.356LeuTrp: 1.356 ± 0.041	1.735LeuTyr: 1.735 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	2.112MetAla: 2.112 ± 0.049	0.112MetCys: 0.112 ± 0.011	0.819MetAsp: 0.819 ± 0.031	0.588MetGlu: 0.588 ± 0.023	0.502MetPhe: 0.502 ± 0.022	1.215MetGly: 1.215 ± 0.035	0.306MetHis: 0.306 ± 0.021	0.791MetIle: 0.791 ± 0.031	0.394MetLys: 0.394 ± 0.018	1.825MetLeu: 1.825 ± 0.049	0.298MetMet: 0.298 ± 0.02	0.467MetAsn: 0.467 ± 0.023	1.174MetPro: 1.174 ± 0.039	0.477MetGln: 0.477 ± 0.022	1.401MetArg: 1.401 ± 0.035	1.447MetSer: 1.447 ± 0.042	1.692MetThr: 1.692 ± 0.045	1.295MetVal: 1.295 ± 0.041	0.224MetTrp: 0.224 ± 0.014	0.249MetTyr: 0.249 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.359AsnAla: 2.359 ± 0.053	0.111AsnCys: 0.111 ± 0.012	1.09AsnAsp: 1.09 ± 0.04	0.907AsnGlu: 0.907 ± 0.03	0.54AsnPhe: 0.54 ± 0.03	1.631AsnGly: 1.631 ± 0.044	0.345AsnHis: 0.345 ± 0.021	0.776AsnIle: 0.776 ± 0.03	0.403AsnLys: 0.403 ± 0.026	1.865AsnLeu: 1.865 ± 0.049	0.301AsnMet: 0.301 ± 0.02	0.435AsnAsn: 0.435 ± 0.023	1.482AsnPro: 1.482 ± 0.042	0.532AsnGln: 0.532 ± 0.025	1.194AsnArg: 1.194 ± 0.037	0.864AsnSer: 0.864 ± 0.034	1.076AsnThr: 1.076 ± 0.039	1.564AsnVal: 1.564 ± 0.042	0.281AsnTrp: 0.281 ± 0.017	0.457AsnTyr: 0.457 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	8.044ProAla: 8.044 ± 0.118	0.206ProCys: 0.206 ± 0.016	4.026ProAsp: 4.026 ± 0.079	3.571ProGlu: 3.571 ± 0.067	1.66ProPhe: 1.66 ± 0.042	5.025ProGly: 5.025 ± 0.09	1.203ProHis: 1.203 ± 0.037	2.052ProIle: 2.052 ± 0.043	0.98ProLys: 0.98 ± 0.036	4.885ProLeu: 4.885 ± 0.075	0.841ProMet: 0.841 ± 0.031	0.921ProAsn: 0.921 ± 0.032	2.381ProPro: 2.381 ± 0.065	1.603ProGln: 1.603 ± 0.039	3.63ProArg: 3.63 ± 0.064	2.845ProSer: 2.845 ± 0.055	3.351ProThr: 3.351 ± 0.056	4.934ProVal: 4.934 ± 0.078	0.903ProTrp: 0.903 ± 0.034	1.026ProTyr: 1.026 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	3.78GlnAla: 3.78 ± 0.062	0.116GlnCys: 0.116 ± 0.012	1.26GlnAsp: 1.26 ± 0.042	1.328GlnGlu: 1.328 ± 0.041	0.883GlnPhe: 0.883 ± 0.031	1.978GlnGly: 1.978 ± 0.043	0.665GlnHis: 0.665 ± 0.025	1.453GlnIle: 1.453 ± 0.043	0.659GlnLys: 0.659 ± 0.025	3.287GlnLeu: 3.287 ± 0.061	0.492GlnMet: 0.492 ± 0.022	0.625GlnAsn: 0.625 ± 0.027	1.544GlnPro: 1.544 ± 0.041	1.237GlnGln: 1.237 ± 0.042	2.644GlnArg: 2.644 ± 0.059	1.458GlnSer: 1.458 ± 0.041	1.657GlnThr: 1.657 ± 0.043	2.378GlnVal: 2.378 ± 0.047	0.524GlnTrp: 0.524 ± 0.025	0.612GlnTyr: 0.612 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	10.002ArgAla: 10.002 ± 0.129	0.361ArgCys: 0.361 ± 0.023	4.628ArgAsp: 4.628 ± 0.078	4.46ArgGlu: 4.46 ± 0.072	2.455ArgPhe: 2.455 ± 0.049	5.803ArgGly: 5.803 ± 0.084	1.722ArgHis: 1.722 ± 0.045	3.628ArgIle: 3.628 ± 0.063	1.404ArgLys: 1.404 ± 0.039	7.63ArgLeu: 7.63 ± 0.097	1.773ArgMet: 1.773 ± 0.046	1.335ArgAsn: 1.335 ± 0.04	3.8ArgPro: 3.8 ± 0.071	2.118ArgGln: 2.118 ± 0.047	7.19ArgArg: 7.19 ± 0.117	3.898ArgSer: 3.898 ± 0.07	4.452ArgThr: 4.452 ± 0.073	6.344ArgVal: 6.344 ± 0.107	1.233ArgTrp: 1.233 ± 0.033	1.671ArgTyr: 1.671 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	6.945SerAla: 6.945 ± 0.102	0.267SerCys: 0.267 ± 0.016	3.119SerAsp: 3.119 ± 0.054	2.44SerGlu: 2.44 ± 0.057	1.759SerPhe: 1.759 ± 0.041	5.428SerGly: 5.428 ± 0.078	0.977SerHis: 0.977 ± 0.033	2.281SerIle: 2.281 ± 0.048	0.961SerLys: 0.961 ± 0.036	5.051SerLeu: 5.051 ± 0.071	1.089SerMet: 1.089 ± 0.034	0.925SerAsn: 0.925 ± 0.038	2.938SerPro: 2.938 ± 0.062	1.317SerGln: 1.317 ± 0.04	3.687SerArg: 3.687 ± 0.062	2.967SerSer: 2.967 ± 0.065	3.274SerThr: 3.274 ± 0.061	4.356SerVal: 4.356 ± 0.075	0.843SerTrp: 0.843 ± 0.028	1.151SerTyr: 1.151 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	8.322ThrAla: 8.322 ± 0.113	0.312ThrCys: 0.312 ± 0.018	3.836ThrAsp: 3.836 ± 0.059	2.964ThrGlu: 2.964 ± 0.061	1.781ThrPhe: 1.781 ± 0.045	5.899ThrGly: 5.899 ± 0.09	1.18ThrHis: 1.18 ± 0.029	2.841ThrIle: 2.841 ± 0.063	1.123ThrLys: 1.123 ± 0.036	6.171ThrLeu: 6.171 ± 0.08	0.944ThrMet: 0.944 ± 0.035	1.151ThrAsn: 1.151 ± 0.045	4.146ThrPro: 4.146 ± 0.073	1.57ThrGln: 1.57 ± 0.042	3.971ThrArg: 3.971 ± 0.069	3.229ThrSer: 3.229 ± 0.053	3.975ThrThr: 3.975 ± 0.072	5.846ThrVal: 5.846 ± 0.105	0.891ThrTrp: 0.891 ± 0.031	1.187ThrTyr: 1.187 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	12.434ValAla: 12.434 ± 0.146	0.534ValCys: 0.534 ± 0.025	5.86ValAsp: 5.86 ± 0.082	4.659ValGlu: 4.659 ± 0.061	2.904ValPhe: 2.904 ± 0.063	7.16ValGly: 7.16 ± 0.103	1.73ValHis: 1.73 ± 0.044	4.301ValIle: 4.301 ± 0.066	1.574ValLys: 1.574 ± 0.047	9.213ValLeu: 9.213 ± 0.128	1.39ValMet: 1.39 ± 0.042	1.693ValAsn: 1.693 ± 0.04	4.742ValPro: 4.742 ± 0.077	2.272ValGln: 2.272 ± 0.051	6.281ValArg: 6.281 ± 0.089	4.724ValSer: 4.724 ± 0.073	6.041ValThr: 6.041 ± 0.101	9.003ValVal: 9.003 ± 0.127	1.211ValTrp: 1.211 ± 0.032	1.559ValTyr: 1.559 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	1.652TrpAla: 1.652 ± 0.041	0.13TrpCys: 0.13 ± 0.011	0.833TrpAsp: 0.833 ± 0.029	0.681TrpGlu: 0.681 ± 0.029	0.537TrpPhe: 0.537 ± 0.022	1.025TrpGly: 1.025 ± 0.035	0.362TrpHis: 0.362 ± 0.019	0.672TrpIle: 0.672 ± 0.024	0.295TrpLys: 0.295 ± 0.021	1.755TrpLeu: 1.755 ± 0.044	0.357TrpMet: 0.357 ± 0.022	0.409TrpAsn: 0.409 ± 0.022	0.744TrpPro: 0.744 ± 0.025	0.589TrpGln: 0.589 ± 0.027	1.358TrpArg: 1.358 ± 0.043	0.91TrpSer: 0.91 ± 0.029	0.945TrpThr: 0.945 ± 0.032	1.236TrpVal: 1.236 ± 0.033	0.371TrpTrp: 0.371 ± 0.022	0.275TrpTyr: 0.275 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.543TyrAla: 2.543 ± 0.054	0.14TyrCys: 0.14 ± 0.013	1.398TyrAsp: 1.398 ± 0.04	1.125TyrGlu: 1.125 ± 0.035	0.675TyrPhe: 0.675 ± 0.028	1.898TyrGly: 1.898 ± 0.048	0.296TyrHis: 0.296 ± 0.016	0.723TyrIle: 0.723 ± 0.03	0.343TyrLys: 0.343 ± 0.02	2.121TyrLeu: 2.121 ± 0.043	0.263TyrMet: 0.263 ± 0.016	0.426TyrAsn: 0.426 ± 0.018	1.056TyrPro: 1.056 ± 0.035	0.596TyrGln: 0.596 ± 0.023	1.72TyrArg: 1.72 ± 0.044	0.951TyrSer: 0.951 ± 0.031	1.144TyrThr: 1.144 ± 0.035	1.606TyrVal: 1.606 ± 0.041	0.3TyrTrp: 0.3 ± 0.017	0.447TyrTyr: 0.447 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3054 proteins (966754 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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