Streptococcus satellite phage Javan16
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZEQ0|A0A4D5ZEQ0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan16 OX=2558548 GN=JavanS16_0016 PE=4 SV=1
MM1 pKa = 7.56 NEE3 pKa = 4.02 LNLTPTQSIILIIVCLVILVLLLHH27 pKa = 6.15 YY28 pKa = 10.5 EE29 pKa = 4.08 SDD31 pKa = 3.58 IEE33 pKa = 5.66 LDD35 pKa = 3.49 LTPQIDD41 pKa = 3.93 EE42 pKa = 4.43 VEE44 pKa = 4.4 EE45 pKa = 4.17 KK46 pKa = 10.18 TKK48 pKa = 9.43 QTTT51 pKa = 3.06
Molecular weight: 5.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.185
IPC2_protein 4.139
IPC_protein 3.923
Toseland 3.77
ProMoST 4.075
Dawson 3.884
Bjellqvist 4.05
Wikipedia 3.795
Rodwell 3.77
Grimsley 3.706
Solomon 3.859
Lehninger 3.808
Nozaki 4.037
DTASelect 4.113
Thurlkill 3.821
EMBOSS 3.808
Sillero 4.037
Patrickios 3.249
IPC_peptide 3.859
IPC2_peptide 4.024
IPC2.peptide.svr19 3.955
Protein with the highest isoelectric point:
>tr|A0A4D5ZJH4|A0A4D5ZJH4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan16 OX=2558548 GN=JavanS16_0013 PE=4 SV=1
MM1 pKa = 7.75 IITVEE6 pKa = 3.73 IAEE9 pKa = 4.24 KK10 pKa = 10.75 VRR12 pKa = 11.84 IKK14 pKa = 10.48 RR15 pKa = 11.84 ARR17 pKa = 11.84 LSMTKK22 pKa = 9.41 TKK24 pKa = 10.61 LSEE27 pKa = 3.94 KK28 pKa = 10.48 LGIARR33 pKa = 11.84 QTLVKK38 pKa = 9.89 IEE40 pKa = 3.92 KK41 pKa = 9.93 GQYY44 pKa = 8.6 KK45 pKa = 9.61 CPKK48 pKa = 9.34 RR49 pKa = 11.84 IYY51 pKa = 9.33 EE52 pKa = 4.66 SVMTWLVEE60 pKa = 4.49 EE61 pKa = 4.4 II62 pKa = 4.06
Molecular weight: 7.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.056
IPC2_protein 9.253
IPC_protein 9.224
Toseland 10.218
ProMoST 9.736
Dawson 10.335
Bjellqvist 9.926
Wikipedia 10.438
Rodwell 10.979
Grimsley 10.365
Solomon 10.365
Lehninger 10.35
Nozaki 10.204
DTASelect 9.926
Thurlkill 10.218
EMBOSS 10.599
Sillero 10.248
Patrickios 10.76
IPC_peptide 10.379
IPC2_peptide 8.434
IPC2.peptide.svr19 8.263
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21
0
21
2781
46
387
132.4
15.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.322 ± 0.482
0.539 ± 0.126
6.149 ± 0.477
7.911 ± 0.678
3.919 ± 0.403
4.171 ± 0.235
1.618 ± 0.25
7.3 ± 0.662
9.097 ± 0.49
9.781 ± 0.534
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.625 ± 0.221
6.257 ± 0.492
2.733 ± 0.218
4.818 ± 0.501
4.171 ± 0.365
6.077 ± 0.373
6.149 ± 0.41
6.149 ± 0.401
1.043 ± 0.216
4.171 ± 0.398
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here