Streptomyces phage GirlPower

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rimavirus; unclassified Rimavirus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6D7Q7|A0A5J6D7Q7_9CAUD Hydrolase OS=Streptomyces phage GirlPower OX=2601688 GN=26 PE=4 SV=1
MM1 pKa = 7.24TQLTWDD7 pKa = 3.17KK8 pKa = 10.91SGEE11 pKa = 4.06RR12 pKa = 11.84FYY14 pKa = 11.04EE15 pKa = 4.02TGVDD19 pKa = 3.46RR20 pKa = 11.84GVLYY24 pKa = 10.5IPDD27 pKa = 3.62SQGAYY32 pKa = 8.84VSGYY36 pKa = 9.37AWNGLTTVTEE46 pKa = 4.44SPSGAEE52 pKa = 4.33SNPQYY57 pKa = 11.32ADD59 pKa = 3.31NIKK62 pKa = 10.1YY63 pKa = 10.68LNLISAEE70 pKa = 4.08EE71 pKa = 3.89FGGTIEE77 pKa = 4.71AFTYY81 pKa = 10.2PDD83 pKa = 3.98EE84 pKa = 5.39FGQCDD89 pKa = 3.72GTAAPTPGVLVGQQSRR105 pKa = 11.84KK106 pKa = 7.76TFGLSYY112 pKa = 9.42RR113 pKa = 11.84TKK115 pKa = 10.7LGNDD119 pKa = 3.95LDD121 pKa = 4.55GQDD124 pKa = 4.02HH125 pKa = 7.09GYY127 pKa = 10.58KK128 pKa = 10.3LHH130 pKa = 6.48LVYY133 pKa = 10.45GALAAPSEE141 pKa = 4.28KK142 pKa = 10.51AYY144 pKa = 9.79ATVNDD149 pKa = 4.08SPEE152 pKa = 4.78AITFSWEE159 pKa = 3.78FTTTPVEE166 pKa = 4.21VGQIAGVDD174 pKa = 3.7YY175 pKa = 10.95KK176 pKa = 10.33PTATLTIDD184 pKa = 3.51STKK187 pKa = 10.49VDD189 pKa = 3.76AGALDD194 pKa = 3.67TLEE197 pKa = 4.08EE198 pKa = 4.13FLYY201 pKa = 9.09GTEE204 pKa = 4.23GTDD207 pKa = 3.99PSLPDD212 pKa = 4.04PATVIAMFSGTVLEE226 pKa = 4.44ATPTEE231 pKa = 4.16PSYY234 pKa = 11.5DD235 pKa = 3.47AATDD239 pKa = 4.19TITIPTVTGVNYY251 pKa = 10.58YY252 pKa = 10.04IGGEE256 pKa = 4.07LQAPGPVIISEE267 pKa = 4.15DD268 pKa = 3.98TIVEE272 pKa = 3.87ARR274 pKa = 11.84PAKK277 pKa = 10.23GYY279 pKa = 10.35KK280 pKa = 10.01FPANVDD286 pKa = 3.55NDD288 pKa = 3.75WLITYY293 pKa = 8.72SS294 pKa = 3.4

Molecular weight: 31.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6D6P3|A0A5J6D6P3_9CAUD Uncharacterized protein OS=Streptomyces phage GirlPower OX=2601688 GN=1 PE=4 SV=1
MM1 pKa = 6.91GTIFGRR7 pKa = 11.84TIVKK11 pKa = 9.61IALRR15 pKa = 11.84VIPEE19 pKa = 3.97SARR22 pKa = 11.84DD23 pKa = 3.33RR24 pKa = 11.84VYY26 pKa = 10.99EE27 pKa = 4.13GTKK30 pKa = 9.19TRR32 pKa = 11.84IARR35 pKa = 11.84FVARR39 pKa = 11.84QIDD42 pKa = 4.07HH43 pKa = 6.21VADD46 pKa = 5.17AIDD49 pKa = 4.07YY50 pKa = 7.61QAHH53 pKa = 6.31PRR55 pKa = 11.84PTAEE59 pKa = 4.0EE60 pKa = 4.0AKK62 pKa = 8.97SWRR65 pKa = 11.84YY66 pKa = 9.43RR67 pKa = 11.84RR68 pKa = 3.72

Molecular weight: 7.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

17156

34

1829

217.2

24.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.163 ± 0.473

0.466 ± 0.084

6.388 ± 0.299

7.344 ± 0.36

3.538 ± 0.2

7.391 ± 0.445

1.813 ± 0.206

5.497 ± 0.149

6.913 ± 0.453

7.991 ± 0.463

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.716 ± 0.144

4.069 ± 0.123

4.127 ± 0.282

3.328 ± 0.153

5.112 ± 0.383

6.01 ± 0.268

6.423 ± 0.349

6.837 ± 0.178

1.405 ± 0.143

3.468 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski