Amino acid dipepetide frequency for Streptomyces phage GirlPower

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.309AlaAla: 11.309 ± 1.722
0.175AlaCys: 0.175 ± 0.105
5.13AlaAsp: 5.13 ± 0.591
6.82AlaGlu: 6.82 ± 0.636
2.681AlaPhe: 2.681 ± 0.325
6.995AlaGly: 6.995 ± 1.465
1.574AlaHis: 1.574 ± 0.284
5.071AlaIle: 5.071 ± 0.527
6.762AlaLys: 6.762 ± 0.71
8.744AlaLeu: 8.744 ± 1.492
3.498AlaMet: 3.498 ± 0.431
2.973AlaAsn: 2.973 ± 0.452
2.74AlaPro: 2.74 ± 0.407
3.556AlaGln: 3.556 ± 0.49
4.838AlaArg: 4.838 ± 0.64
3.614AlaSer: 3.614 ± 0.645
5.538AlaThr: 5.538 ± 0.456
5.421AlaVal: 5.421 ± 0.67
0.933AlaTrp: 0.933 ± 0.278
3.264AlaTyr: 3.264 ± 0.414
0.0AlaXaa: 0.0 ± 0.0
Cys
0.291CysAla: 0.291 ± 0.163
0.0CysCys: 0.0 ± 0.0
0.35CysAsp: 0.35 ± 0.137
0.408CysGlu: 0.408 ± 0.173
0.117CysPhe: 0.117 ± 0.08
0.291CysGly: 0.291 ± 0.152
0.058CysHis: 0.058 ± 0.056
0.175CysIle: 0.175 ± 0.102
0.233CysLys: 0.233 ± 0.102
0.874CysLeu: 0.874 ± 0.277
0.058CysMet: 0.058 ± 0.06
0.233CysAsn: 0.233 ± 0.123
0.175CysPro: 0.175 ± 0.13
0.175CysGln: 0.175 ± 0.1
0.117CysArg: 0.117 ± 0.096
0.291CysSer: 0.291 ± 0.125
0.233CysThr: 0.233 ± 0.106
0.291CysVal: 0.291 ± 0.133
0.175CysTrp: 0.175 ± 0.09
0.117CysTyr: 0.117 ± 0.079
0.0CysXaa: 0.0 ± 0.0
Asp
6.47AspAla: 6.47 ± 0.609
0.291AspCys: 0.291 ± 0.141
3.614AspAsp: 3.614 ± 0.537
5.713AspGlu: 5.713 ± 0.742
2.565AspPhe: 2.565 ± 0.398
5.654AspGly: 5.654 ± 0.639
1.108AspHis: 1.108 ± 0.256
3.964AspIle: 3.964 ± 0.645
3.323AspLys: 3.323 ± 0.358
4.838AspLeu: 4.838 ± 0.633
1.924AspMet: 1.924 ± 0.333
2.623AspAsn: 2.623 ± 0.479
3.964AspPro: 3.964 ± 0.716
2.099AspGln: 2.099 ± 0.317
3.206AspArg: 3.206 ± 0.459
3.031AspSer: 3.031 ± 0.393
3.148AspThr: 3.148 ± 0.407
2.856AspVal: 2.856 ± 0.359
1.632AspTrp: 1.632 ± 0.333
1.865AspTyr: 1.865 ± 0.331
0.0AspXaa: 0.0 ± 0.0
Glu
5.538GluAla: 5.538 ± 0.674
0.233GluCys: 0.233 ± 0.12
4.722GluAsp: 4.722 ± 0.604
6.937GluGlu: 6.937 ± 0.98
3.498GluPhe: 3.498 ± 0.568
5.188GluGly: 5.188 ± 0.53
1.166GluHis: 1.166 ± 0.291
4.08GluIle: 4.08 ± 0.445
4.838GluLys: 4.838 ± 0.643
6.179GluLeu: 6.179 ± 0.669
3.148GluMet: 3.148 ± 0.418
3.089GluAsn: 3.089 ± 0.478
2.273GluPro: 2.273 ± 0.463
2.565GluGln: 2.565 ± 0.356
4.197GluArg: 4.197 ± 0.552
3.264GluSer: 3.264 ± 0.601
4.197GluThr: 4.197 ± 0.472
5.596GluVal: 5.596 ± 0.704
0.816GluTrp: 0.816 ± 0.188
1.924GluTyr: 1.924 ± 0.266
0.0GluXaa: 0.0 ± 0.0
Phe
2.915PheAla: 2.915 ± 0.386
0.35PheCys: 0.35 ± 0.164
3.498PheAsp: 3.498 ± 0.513
2.273PheGlu: 2.273 ± 0.347
1.457PhePhe: 1.457 ± 0.272
2.507PheGly: 2.507 ± 0.437
0.7PheHis: 0.7 ± 0.191
1.69PheIle: 1.69 ± 0.425
2.215PheLys: 2.215 ± 0.428
2.215PheLeu: 2.215 ± 0.348
0.816PheMet: 0.816 ± 0.178
1.457PheAsn: 1.457 ± 0.259
1.166PhePro: 1.166 ± 0.25
1.516PheGln: 1.516 ± 0.288
2.565PheArg: 2.565 ± 0.442
1.982PheSer: 1.982 ± 0.452
2.39PheThr: 2.39 ± 0.411
2.332PheVal: 2.332 ± 0.355
0.408PheTrp: 0.408 ± 0.164
0.933PheTyr: 0.933 ± 0.284
0.0PheXaa: 0.0 ± 0.0
Gly
6.179GlyAla: 6.179 ± 1.013
0.408GlyCys: 0.408 ± 0.155
5.013GlyAsp: 5.013 ± 0.572
4.197GlyGlu: 4.197 ± 0.513
3.381GlyPhe: 3.381 ± 0.483
4.897GlyGly: 4.897 ± 0.643
1.108GlyHis: 1.108 ± 0.294
4.488GlyIle: 4.488 ± 0.896
4.722GlyLys: 4.722 ± 0.486
6.296GlyLeu: 6.296 ± 1.613
2.215GlyMet: 2.215 ± 0.277
2.681GlyAsn: 2.681 ± 0.462
2.273GlyPro: 2.273 ± 0.422
1.982GlyGln: 1.982 ± 0.431
2.565GlyArg: 2.565 ± 0.347
4.78GlySer: 4.78 ± 0.745
5.596GlyThr: 5.596 ± 0.578
6.587GlyVal: 6.587 ± 0.835
0.933GlyTrp: 0.933 ± 0.225
3.614GlyTyr: 3.614 ± 0.603
0.0GlyXaa: 0.0 ± 0.0
His
1.224HisAla: 1.224 ± 0.305
0.175HisCys: 0.175 ± 0.097
1.049HisAsp: 1.049 ± 0.202
1.224HisGlu: 1.224 ± 0.287
0.35HisPhe: 0.35 ± 0.149
1.399HisGly: 1.399 ± 0.286
0.583HisHis: 0.583 ± 0.177
1.282HisIle: 1.282 ± 0.329
1.049HisLys: 1.049 ± 0.263
1.049HisLeu: 1.049 ± 0.239
0.408HisMet: 0.408 ± 0.146
0.758HisAsn: 0.758 ± 0.231
1.108HisPro: 1.108 ± 0.261
0.525HisGln: 0.525 ± 0.222
0.816HisArg: 0.816 ± 0.24
1.457HisSer: 1.457 ± 0.279
0.641HisThr: 0.641 ± 0.174
1.457HisVal: 1.457 ± 0.315
0.35HisTrp: 0.35 ± 0.174
1.224HisTyr: 1.224 ± 0.238
0.0HisXaa: 0.0 ± 0.0
Ile
5.246IleAla: 5.246 ± 0.548
0.525IleCys: 0.525 ± 0.183
4.722IleAsp: 4.722 ± 0.546
4.488IleGlu: 4.488 ± 0.524
2.681IlePhe: 2.681 ± 0.392
3.089IleGly: 3.089 ± 0.695
1.049IleHis: 1.049 ± 0.281
1.924IleIle: 1.924 ± 0.307
3.731IleLys: 3.731 ± 0.513
4.663IleLeu: 4.663 ± 0.51
0.991IleMet: 0.991 ± 0.199
2.099IleAsn: 2.099 ± 0.322
2.39IlePro: 2.39 ± 0.379
1.574IleGln: 1.574 ± 0.234
2.623IleArg: 2.623 ± 0.381
3.672IleSer: 3.672 ± 0.55
3.847IleThr: 3.847 ± 0.563
3.614IleVal: 3.614 ± 0.681
0.233IleTrp: 0.233 ± 0.15
1.807IleTyr: 1.807 ± 0.259
0.0IleXaa: 0.0 ± 0.0
Lys
6.412LysAla: 6.412 ± 0.71
0.175LysCys: 0.175 ± 0.099
3.847LysAsp: 3.847 ± 0.663
4.139LysGlu: 4.139 ± 0.61
1.924LysPhe: 1.924 ± 0.314
4.955LysGly: 4.955 ± 1.057
1.282LysHis: 1.282 ± 0.329
3.847LysIle: 3.847 ± 0.571
6.82LysLys: 6.82 ± 0.812
6.412LysLeu: 6.412 ± 0.676
2.099LysMet: 2.099 ± 0.462
2.448LysAsn: 2.448 ± 0.43
2.681LysPro: 2.681 ± 0.46
2.856LysGln: 2.856 ± 0.357
3.439LysArg: 3.439 ± 0.488
4.722LysSer: 4.722 ± 0.673
3.556LysThr: 3.556 ± 0.446
4.197LysVal: 4.197 ± 0.455
0.991LysTrp: 0.991 ± 0.273
2.332LysTyr: 2.332 ± 0.281
0.0LysXaa: 0.0 ± 0.0
Leu
8.161LeuAla: 8.161 ± 1.278
0.291LeuCys: 0.291 ± 0.116
4.955LeuAsp: 4.955 ± 0.51
6.645LeuGlu: 6.645 ± 0.547
2.157LeuPhe: 2.157 ± 0.36
5.771LeuGly: 5.771 ± 0.965
1.574LeuHis: 1.574 ± 0.296
3.847LeuIle: 3.847 ± 0.722
5.654LeuLys: 5.654 ± 0.563
6.121LeuLeu: 6.121 ± 0.712
2.215LeuMet: 2.215 ± 0.301
3.498LeuAsn: 3.498 ± 0.476
2.565LeuPro: 2.565 ± 0.454
2.681LeuGln: 2.681 ± 0.397
4.372LeuArg: 4.372 ± 0.547
4.663LeuSer: 4.663 ± 0.513
5.538LeuThr: 5.538 ± 0.629
5.596LeuVal: 5.596 ± 0.73
0.991LeuTrp: 0.991 ± 0.193
2.623LeuTyr: 2.623 ± 0.356
0.0LeuXaa: 0.0 ± 0.0
Met
2.681MetAla: 2.681 ± 0.373
0.117MetCys: 0.117 ± 0.077
1.807MetAsp: 1.807 ± 0.255
1.632MetGlu: 1.632 ± 0.273
1.166MetPhe: 1.166 ± 0.237
2.04MetGly: 2.04 ± 0.286
0.233MetHis: 0.233 ± 0.1
0.874MetIle: 0.874 ± 0.299
1.865MetLys: 1.865 ± 0.369
2.448MetLeu: 2.448 ± 0.356
0.35MetMet: 0.35 ± 0.175
1.341MetAsn: 1.341 ± 0.273
1.341MetPro: 1.341 ± 0.354
0.641MetGln: 0.641 ± 0.18
1.282MetArg: 1.282 ± 0.299
2.332MetSer: 2.332 ± 0.35
2.273MetThr: 2.273 ± 0.337
1.574MetVal: 1.574 ± 0.264
0.35MetTrp: 0.35 ± 0.155
0.816MetTyr: 0.816 ± 0.201
0.0MetXaa: 0.0 ± 0.0
Asn
3.439AsnAla: 3.439 ± 0.467
0.058AsnCys: 0.058 ± 0.059
2.448AsnAsp: 2.448 ± 0.381
2.973AsnGlu: 2.973 ± 0.378
0.933AsnPhe: 0.933 ± 0.281
3.264AsnGly: 3.264 ± 0.386
0.933AsnHis: 0.933 ± 0.197
2.157AsnIle: 2.157 ± 0.315
2.099AsnLys: 2.099 ± 0.399
2.798AsnLeu: 2.798 ± 0.401
0.933AsnMet: 0.933 ± 0.197
1.749AsnAsn: 1.749 ± 0.311
2.565AsnPro: 2.565 ± 0.395
1.224AsnGln: 1.224 ± 0.23
3.381AsnArg: 3.381 ± 0.523
2.565AsnSer: 2.565 ± 0.45
2.74AsnThr: 2.74 ± 0.302
2.681AsnVal: 2.681 ± 0.352
0.233AsnTrp: 0.233 ± 0.111
1.516AsnTyr: 1.516 ± 0.258
0.0AsnXaa: 0.0 ± 0.0
Pro
2.448ProAla: 2.448 ± 0.394
0.291ProCys: 0.291 ± 0.138
3.847ProAsp: 3.847 ± 0.707
3.731ProGlu: 3.731 ± 0.63
1.341ProPhe: 1.341 ± 0.246
2.507ProGly: 2.507 ± 0.373
0.583ProHis: 0.583 ± 0.202
1.749ProIle: 1.749 ± 0.262
2.915ProLys: 2.915 ± 0.463
2.273ProLeu: 2.273 ± 0.281
0.874ProMet: 0.874 ± 0.243
1.399ProAsn: 1.399 ± 0.307
2.04ProPro: 2.04 ± 0.417
0.933ProGln: 0.933 ± 0.26
1.865ProArg: 1.865 ± 0.514
3.381ProSer: 3.381 ± 0.471
3.498ProThr: 3.498 ± 0.503
3.847ProVal: 3.847 ± 0.459
0.466ProTrp: 0.466 ± 0.137
1.282ProTyr: 1.282 ± 0.301
0.0ProXaa: 0.0 ± 0.0
Gln
3.556GlnAla: 3.556 ± 0.395
0.117GlnCys: 0.117 ± 0.078
1.574GlnAsp: 1.574 ± 0.348
2.099GlnGlu: 2.099 ± 0.33
1.166GlnPhe: 1.166 ± 0.262
2.565GlnGly: 2.565 ± 0.368
1.166GlnHis: 1.166 ± 0.336
2.448GlnIle: 2.448 ± 0.394
2.157GlnLys: 2.157 ± 0.342
3.148GlnLeu: 3.148 ± 0.481
0.991GlnMet: 0.991 ± 0.2
0.991GlnAsn: 0.991 ± 0.218
0.991GlnPro: 0.991 ± 0.228
1.166GlnGln: 1.166 ± 0.333
1.574GlnArg: 1.574 ± 0.312
1.865GlnSer: 1.865 ± 0.327
2.099GlnThr: 2.099 ± 0.437
1.982GlnVal: 1.982 ± 0.319
0.35GlnTrp: 0.35 ± 0.155
1.282GlnTyr: 1.282 ± 0.31
0.0GlnXaa: 0.0 ± 0.0
Arg
3.789ArgAla: 3.789 ± 0.601
0.35ArgCys: 0.35 ± 0.155
3.089ArgAsp: 3.089 ± 0.523
4.314ArgGlu: 4.314 ± 0.653
1.108ArgPhe: 1.108 ± 0.207
3.089ArgGly: 3.089 ± 0.554
0.816ArgHis: 0.816 ± 0.25
2.74ArgIle: 2.74 ± 0.462
4.547ArgLys: 4.547 ± 0.53
4.547ArgLeu: 4.547 ± 0.673
1.632ArgMet: 1.632 ± 0.351
2.507ArgAsn: 2.507 ± 0.44
2.157ArgPro: 2.157 ± 0.419
1.69ArgGln: 1.69 ± 0.272
3.206ArgArg: 3.206 ± 0.647
3.498ArgSer: 3.498 ± 0.465
2.915ArgThr: 2.915 ± 0.387
2.798ArgVal: 2.798 ± 0.384
0.7ArgTrp: 0.7 ± 0.241
1.632ArgTyr: 1.632 ± 0.29
0.0ArgXaa: 0.0 ± 0.0
Ser
5.771SerAla: 5.771 ± 0.941
0.175SerCys: 0.175 ± 0.133
3.964SerAsp: 3.964 ± 0.493
4.43SerGlu: 4.43 ± 0.547
2.332SerPhe: 2.332 ± 0.448
4.955SerGly: 4.955 ± 0.521
0.991SerHis: 0.991 ± 0.27
3.089SerIle: 3.089 ± 0.443
4.197SerLys: 4.197 ± 0.619
3.964SerLeu: 3.964 ± 0.48
1.108SerMet: 1.108 ± 0.248
2.507SerAsn: 2.507 ± 0.351
2.623SerPro: 2.623 ± 0.362
2.273SerGln: 2.273 ± 0.384
2.681SerArg: 2.681 ± 0.528
4.314SerSer: 4.314 ± 0.794
3.614SerThr: 3.614 ± 0.488
4.08SerVal: 4.08 ± 0.628
1.282SerTrp: 1.282 ± 0.337
1.749SerTyr: 1.749 ± 0.283
0.0SerXaa: 0.0 ± 0.0
Thr
5.829ThrAla: 5.829 ± 0.767
0.233ThrCys: 0.233 ± 0.107
3.672ThrAsp: 3.672 ± 0.517
3.089ThrGlu: 3.089 ± 0.413
2.565ThrPhe: 2.565 ± 0.335
5.654ThrGly: 5.654 ± 0.635
1.399ThrHis: 1.399 ± 0.281
4.488ThrIle: 4.488 ± 0.504
4.08ThrLys: 4.08 ± 0.373
4.78ThrLeu: 4.78 ± 0.524
1.399ThrMet: 1.399 ± 0.292
1.807ThrAsn: 1.807 ± 0.266
3.906ThrPro: 3.906 ± 0.549
1.69ThrGln: 1.69 ± 0.291
2.623ThrArg: 2.623 ± 0.422
3.556ThrSer: 3.556 ± 0.503
5.305ThrThr: 5.305 ± 0.836
5.13ThrVal: 5.13 ± 0.486
1.049ThrTrp: 1.049 ± 0.266
1.982ThrTyr: 1.982 ± 0.342
0.0ThrXaa: 0.0 ± 0.0
Val
6.004ValAla: 6.004 ± 0.648
0.35ValCys: 0.35 ± 0.139
3.847ValAsp: 3.847 ± 0.435
4.663ValGlu: 4.663 ± 0.569
2.04ValPhe: 2.04 ± 0.439
5.363ValGly: 5.363 ± 0.624
0.933ValHis: 0.933 ± 0.238
4.197ValIle: 4.197 ± 0.42
4.722ValLys: 4.722 ± 0.472
5.363ValLeu: 5.363 ± 0.61
1.574ValMet: 1.574 ± 0.276
3.206ValAsn: 3.206 ± 0.397
2.681ValPro: 2.681 ± 0.435
1.982ValGln: 1.982 ± 0.297
4.139ValArg: 4.139 ± 0.547
3.964ValSer: 3.964 ± 0.419
4.663ValThr: 4.663 ± 0.632
4.255ValVal: 4.255 ± 0.407
1.516ValTrp: 1.516 ± 0.272
2.856ValTyr: 2.856 ± 0.535
0.0ValXaa: 0.0 ± 0.0
Trp
0.874TrpAla: 0.874 ± 0.201
0.233TrpCys: 0.233 ± 0.116
0.991TrpAsp: 0.991 ± 0.273
1.224TrpGlu: 1.224 ± 0.236
0.291TrpPhe: 0.291 ± 0.135
1.632TrpGly: 1.632 ± 0.355
0.291TrpHis: 0.291 ± 0.119
0.466TrpIle: 0.466 ± 0.171
0.991TrpLys: 0.991 ± 0.221
1.049TrpLeu: 1.049 ± 0.257
0.408TrpMet: 0.408 ± 0.176
0.933TrpAsn: 0.933 ± 0.282
0.291TrpPro: 0.291 ± 0.123
0.175TrpGln: 0.175 ± 0.091
0.35TrpArg: 0.35 ± 0.17
0.933TrpSer: 0.933 ± 0.255
1.049TrpThr: 1.049 ± 0.275
1.282TrpVal: 1.282 ± 0.372
0.117TrpTrp: 0.117 ± 0.075
0.466TrpTyr: 0.466 ± 0.172
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.498TyrAla: 3.498 ± 0.532
0.117TyrCys: 0.117 ± 0.07
1.749TyrAsp: 1.749 ± 0.326
2.448TyrGlu: 2.448 ± 0.423
1.632TyrPhe: 1.632 ± 0.388
2.099TyrGly: 2.099 ± 0.388
0.583TyrHis: 0.583 ± 0.172
2.39TyrIle: 2.39 ± 0.34
2.215TyrLys: 2.215 ± 0.431
2.157TyrLeu: 2.157 ± 0.352
0.466TyrMet: 0.466 ± 0.146
2.39TyrAsn: 2.39 ± 0.31
1.341TyrPro: 1.341 ± 0.309
1.982TyrGln: 1.982 ± 0.348
1.282TyrArg: 1.282 ± 0.307
2.215TyrSer: 2.215 ± 0.356
1.399TyrThr: 1.399 ± 0.387
2.798TyrVal: 2.798 ± 0.304
0.525TyrTrp: 0.525 ± 0.184
1.399TyrTyr: 1.399 ± 0.375
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (17156 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski