Phytoactinopolyspora alkaliphila

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Jiangellales; Jiangellaceae; Phytoactinopolyspora

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4852 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N9YTC5|A0A6N9YTC5_9ACTN Cysteine desulfurase OS=Phytoactinopolyspora alkaliphila OX=1783498 GN=G1H11_23615 PE=3 SV=1
MM1 pKa = 5.6TTKK4 pKa = 10.42RR5 pKa = 11.84RR6 pKa = 11.84GAAFLAVAAASALVLAACGGDD27 pKa = 4.18DD28 pKa = 5.22ADD30 pKa = 4.58EE31 pKa = 4.64TPADD35 pKa = 4.35DD36 pKa = 5.47DD37 pKa = 4.11GGAAEE42 pKa = 4.74EE43 pKa = 4.7QPDD46 pKa = 4.36PDD48 pKa = 4.02TGEE51 pKa = 4.37GPSDD55 pKa = 3.79VEE57 pKa = 3.92VVYY60 pKa = 10.41AAEE63 pKa = 4.28QEE65 pKa = 4.3FTNYY69 pKa = 10.61ANNTSEE75 pKa = 4.87GNLLANTHH83 pKa = 5.66VLNQVKK89 pKa = 10.4GGFWNYY95 pKa = 10.35GPDD98 pKa = 3.58GLVVPDD104 pKa = 4.71EE105 pKa = 4.31EE106 pKa = 4.64FGTYY110 pKa = 10.69DD111 pKa = 4.63LVSDD115 pKa = 4.69DD116 pKa = 4.0PQTIEE121 pKa = 5.07FNINPDD127 pKa = 3.79AVWSDD132 pKa = 4.06GEE134 pKa = 5.26PIDD137 pKa = 5.81CDD139 pKa = 4.29DD140 pKa = 5.77FLLGWAAQSGYY151 pKa = 9.91YY152 pKa = 8.26EE153 pKa = 4.98HH154 pKa = 7.15PTEE157 pKa = 4.24TTADD161 pKa = 3.91GEE163 pKa = 4.33PATLFSTAGTTGYY176 pKa = 11.04ADD178 pKa = 3.39WVKK181 pKa = 10.48PDD183 pKa = 4.7CEE185 pKa = 4.96PGDD188 pKa = 3.47KK189 pKa = 10.43TITVEE194 pKa = 4.16YY195 pKa = 8.09EE196 pKa = 3.97TVNASWVLYY205 pKa = 8.96TAVDD209 pKa = 3.97MPAHH213 pKa = 4.91VVARR217 pKa = 11.84EE218 pKa = 3.75GGMASGDD225 pKa = 3.75EE226 pKa = 4.5LIEE229 pKa = 4.85AIRR232 pKa = 11.84NDD234 pKa = 5.23DD235 pKa = 3.7IDD237 pKa = 4.61ALLPAAEE244 pKa = 4.79FYY246 pKa = 9.79NTGFVMSPGEE256 pKa = 4.19LLPEE260 pKa = 4.48EE261 pKa = 5.25IIPSSGRR268 pKa = 11.84YY269 pKa = 9.37KK270 pKa = 10.2LTDD273 pKa = 3.12WQAGQSITMEE283 pKa = 4.03YY284 pKa = 10.07NEE286 pKa = 5.19NYY288 pKa = 9.63WGTPPAARR296 pKa = 11.84TIIFRR301 pKa = 11.84FLDD304 pKa = 3.73ANQQAQALQNQEE316 pKa = 3.74IDD318 pKa = 3.62IMDD321 pKa = 4.59PQPSTDD327 pKa = 4.18LVNQLEE333 pKa = 4.41GMAGVEE339 pKa = 4.03VHH341 pKa = 6.12VDD343 pKa = 3.19EE344 pKa = 5.09SFTYY348 pKa = 10.16EE349 pKa = 3.91HH350 pKa = 6.92VDD352 pKa = 3.64FNLRR356 pKa = 11.84GDD358 pKa = 4.16HH359 pKa = 6.47YY360 pKa = 11.58FNDD363 pKa = 3.67PNLRR367 pKa = 11.84RR368 pKa = 11.84SFGLCLPRR376 pKa = 11.84QLMVEE381 pKa = 4.11NLIHH385 pKa = 6.19PQNPDD390 pKa = 3.24AEE392 pKa = 4.53VLNARR397 pKa = 11.84LTYY400 pKa = 10.49SFQPDD405 pKa = 3.26YY406 pKa = 11.48DD407 pKa = 3.75FQIDD411 pKa = 3.77GNGWEE416 pKa = 5.11DD417 pKa = 4.21FAEE420 pKa = 3.92QDD422 pKa = 2.75IDD424 pKa = 3.4EE425 pKa = 4.38SRR427 pKa = 11.84RR428 pKa = 11.84LLEE431 pKa = 4.18EE432 pKa = 5.14ADD434 pKa = 3.61MVGTEE439 pKa = 3.69VRR441 pKa = 11.84IVYY444 pKa = 9.09TEE446 pKa = 3.88ANPRR450 pKa = 11.84RR451 pKa = 11.84VDD453 pKa = 3.56QVALIRR459 pKa = 11.84DD460 pKa = 3.85ACNQAGWDD468 pKa = 3.78VVDD471 pKa = 5.33AGSADD476 pKa = 3.62PFGVEE481 pKa = 4.05IPDD484 pKa = 3.8GNFDD488 pKa = 3.23VAMYY492 pKa = 10.36AWIGSGYY499 pKa = 9.93VAGTASTFMTPSSCTPEE516 pKa = 3.9GTGNNSQCYY525 pKa = 8.78SSEE528 pKa = 4.19VVDD531 pKa = 4.04DD532 pKa = 4.98LYY534 pKa = 11.39RR535 pKa = 11.84EE536 pKa = 4.19LLQEE540 pKa = 4.02VDD542 pKa = 2.97VDD544 pKa = 3.84AQRR547 pKa = 11.84EE548 pKa = 4.18LVKK551 pKa = 10.79QIEE554 pKa = 4.45TQLWADD560 pKa = 4.51LPTIPLFTHH569 pKa = 7.28PYY571 pKa = 7.97LLAWAEE577 pKa = 4.14DD578 pKa = 4.07VEE580 pKa = 5.52GITPNPTQQGVTSTKK595 pKa = 10.46HH596 pKa = 3.81EE597 pKa = 4.31WNRR600 pKa = 11.84ALL602 pKa = 4.83

Molecular weight: 66.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N9YI09|A0A6N9YI09_9ACTN Dolichyl-phosphate-mannose--protein mannosyltransferase OS=Phytoactinopolyspora alkaliphila OX=1783498 GN=G1H11_04600 PE=3 SV=1
MM1 pKa = 7.28GSVVKK6 pKa = 10.49KK7 pKa = 9.45RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.48RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.64HH17 pKa = 5.77RR18 pKa = 11.84KK19 pKa = 7.99LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84QGKK33 pKa = 8.88

Molecular weight: 4.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4852

0

4852

1624139

29

2147

334.7

35.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.797 ± 0.041

0.677 ± 0.009

6.62 ± 0.035

5.992 ± 0.027

2.876 ± 0.021

9.113 ± 0.031

2.281 ± 0.018

3.866 ± 0.023

1.622 ± 0.021

9.868 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.943 ± 0.013

1.912 ± 0.018

5.677 ± 0.023

2.769 ± 0.016

7.802 ± 0.036

5.54 ± 0.023

5.908 ± 0.024

9.067 ± 0.034

1.567 ± 0.013

2.102 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski