Salinibacillus xinjiangensis
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3701 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G1X7Y2|A0A6G1X7Y2_9BACI Peptide ABC transporter substrate-binding protein OS=Salinibacillus xinjiangensis OX=1229268 GN=GH754_12215 PE=4 SV=1
MM1 pKa = 7.51 PAFVGAVKK9 pKa = 10.56 VNSVGSSSIFHH20 pKa = 6.72 IGDD23 pKa = 3.85 VYY25 pKa = 11.61 AMSPTSSAKK34 pKa = 9.11 TFAGGGSFNTGDD46 pKa = 3.67 GLSINVDD53 pKa = 3.08 QSYY56 pKa = 8.68 TYY58 pKa = 10.07 VTDD61 pKa = 4.09 SDD63 pKa = 4.44 TFDD66 pKa = 3.15 QTIVGNAA73 pKa = 3.12
Molecular weight: 7.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.784
IPC2_protein 4.05
IPC_protein 3.897
Toseland 3.668
ProMoST 4.139
Dawson 3.948
Bjellqvist 4.113
Wikipedia 3.986
Rodwell 3.732
Grimsley 3.592
Solomon 3.91
Lehninger 3.872
Nozaki 4.101
DTASelect 4.406
Thurlkill 3.795
EMBOSS 3.986
Sillero 4.037
Patrickios 1.977
IPC_peptide 3.897
IPC2_peptide 3.999
IPC2.peptide.svr19 3.927
Protein with the highest isoelectric point:
>tr|A0A6G1XAC1|A0A6G1XAC1_9BACI Membrane protein insertase YidC OS=Salinibacillus xinjiangensis OX=1229268 GN=yidC PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.7 QPKK8 pKa = 8.77 KK9 pKa = 8.07 RR10 pKa = 11.84 KK11 pKa = 8.85 RR12 pKa = 11.84 SKK14 pKa = 9.05 VHH16 pKa = 5.83 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MSSKK25 pKa = 10.57 NGRR28 pKa = 11.84 KK29 pKa = 8.91 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.3 GRR39 pKa = 11.84 KK40 pKa = 8.62 NLSAA44 pKa = 4.67
Molecular weight: 5.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.435
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.457
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.179
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.041
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3701
0
3701
1049479
19
1509
283.6
31.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.431 ± 0.042
0.626 ± 0.012
5.38 ± 0.033
7.872 ± 0.054
4.552 ± 0.038
6.843 ± 0.045
2.186 ± 0.02
7.737 ± 0.041
6.858 ± 0.043
9.543 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.807 ± 0.019
4.528 ± 0.034
3.605 ± 0.026
4.152 ± 0.032
3.896 ± 0.03
5.97 ± 0.028
5.358 ± 0.028
7.112 ± 0.033
1.023 ± 0.016
3.522 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here