Salmonella phage slyngel
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G8RA07|A0A6G8RA07_9CAUD Putative helicase OS=Salmonella phage slyngel OX=2713321 GN=slyngel_55 PE=4 SV=1
MM1 pKa = 6.55 MQSTVIKK8 pKa = 10.32 FLGSDD13 pKa = 3.48 CEE15 pKa = 4.22 EE16 pKa = 4.06 VNEE19 pKa = 4.66 CFTAGKK25 pKa = 9.79 RR26 pKa = 11.84 YY27 pKa = 9.13 HH28 pKa = 7.13 ADD30 pKa = 2.88 VSSAGNYY37 pKa = 8.25 FVYY40 pKa = 10.17 DD41 pKa = 3.53 DD42 pKa = 5.7 HH43 pKa = 8.4 GDD45 pKa = 3.21 TWVIDD50 pKa = 3.61 QDD52 pKa = 4.07 DD53 pKa = 4.13 EE54 pKa = 4.46 EE55 pKa = 5.24 FSVEE59 pKa = 3.83
Molecular weight: 6.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.91
IPC_protein 3.834
Toseland 3.643
ProMoST 3.961
Dawson 3.834
Bjellqvist 4.062
Wikipedia 3.77
Rodwell 3.668
Grimsley 3.554
Solomon 3.808
Lehninger 3.757
Nozaki 3.961
DTASelect 4.151
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.961
Patrickios 0.769
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.894
Protein with the highest isoelectric point:
>tr|A0A6G8RA05|A0A6G8RA05_9CAUD Uncharacterized protein OS=Salmonella phage slyngel OX=2713321 GN=slyngel_46 PE=4 SV=1
MM1 pKa = 7.74 LEE3 pKa = 4.19 LKK5 pKa = 10.36 DD6 pKa = 3.44 IRR8 pKa = 11.84 VGTKK12 pKa = 9.97 FRR14 pKa = 11.84 VTWADD19 pKa = 3.79 EE20 pKa = 4.17 YY21 pKa = 11.34 CGVSKK26 pKa = 10.82 GQVVTVDD33 pKa = 4.35 SIYY36 pKa = 10.76 HH37 pKa = 5.68 GCSKK41 pKa = 10.64 DD42 pKa = 3.38 FRR44 pKa = 11.84 RR45 pKa = 11.84 PRR47 pKa = 11.84 IKK49 pKa = 10.15 NGYY52 pKa = 9.12 IITRR56 pKa = 11.84 RR57 pKa = 11.84 LGFDD61 pKa = 3.72 HH62 pKa = 6.74 YY63 pKa = 11.16 CVVATQGVLIEE74 pKa = 4.4 LEE76 pKa = 4.52 RR77 pKa = 11.84 ISDD80 pKa = 3.53 HH81 pKa = 6.68 RR82 pKa = 11.84 GCHH85 pKa = 4.86 VKK87 pKa = 9.66 TMKK90 pKa = 10.33 IPFLRR95 pKa = 11.84 ARR97 pKa = 11.84 YY98 pKa = 8.42 DD99 pKa = 3.17 ARR101 pKa = 11.84 RR102 pKa = 11.84 MRR104 pKa = 11.84 RR105 pKa = 11.84 LARR108 pKa = 11.84 NAIKK112 pKa = 10.47 LKK114 pKa = 10.62 KK115 pKa = 9.46 PGSALYY121 pKa = 8.45 GTYY124 pKa = 10.32 KK125 pKa = 10.88 GIARR129 pKa = 11.84 NAGKK133 pKa = 10.28
Molecular weight: 15.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.706
IPC_protein 10.248
Toseland 10.496
ProMoST 10.189
Dawson 10.628
Bjellqvist 10.321
Wikipedia 10.818
Rodwell 10.979
Grimsley 10.687
Solomon 10.687
Lehninger 10.657
Nozaki 10.496
DTASelect 10.306
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.555
Patrickios 10.687
IPC_peptide 10.687
IPC2_peptide 9.297
IPC2.peptide.svr19 8.601
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
80
0
80
15180
43
1141
189.8
21.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.024 ± 0.412
1.37 ± 0.172
6.238 ± 0.272
6.647 ± 0.265
3.926 ± 0.167
7.569 ± 0.24
1.772 ± 0.218
6.765 ± 0.194
7.385 ± 0.375
6.397 ± 0.205
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.136 ± 0.177
4.743 ± 0.209
3.379 ± 0.201
3.999 ± 0.275
5.0 ± 0.202
6.14 ± 0.269
5.402 ± 0.247
6.93 ± 0.234
1.456 ± 0.106
3.722 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here