Amino acid dipepetide frequency for Salmonella phage slyngel

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.193AlaAla: 6.193 ± 0.95
0.922AlaCys: 0.922 ± 0.244
4.612AlaAsp: 4.612 ± 0.704
5.073AlaGlu: 5.073 ± 0.617
2.899AlaPhe: 2.899 ± 0.478
5.402AlaGly: 5.402 ± 0.547
1.186AlaHis: 1.186 ± 0.288
6.061AlaIle: 6.061 ± 0.78
6.259AlaLys: 6.259 ± 0.953
6.39AlaLeu: 6.39 ± 0.662
2.899AlaMet: 2.899 ± 0.418
3.887AlaAsn: 3.887 ± 0.588
2.108AlaPro: 2.108 ± 0.332
3.228AlaGln: 3.228 ± 0.565
4.15AlaArg: 4.15 ± 0.559
4.941AlaSer: 4.941 ± 0.582
4.15AlaThr: 4.15 ± 0.691
5.995AlaVal: 5.995 ± 0.821
1.186AlaTrp: 1.186 ± 0.248
2.701AlaTyr: 2.701 ± 0.484
0.0AlaXaa: 0.0 ± 0.0
Cys
1.186CysAla: 1.186 ± 0.301
0.264CysCys: 0.264 ± 0.24
1.515CysAsp: 1.515 ± 0.295
1.054CysGlu: 1.054 ± 0.304
0.264CysPhe: 0.264 ± 0.136
1.581CysGly: 1.581 ± 0.483
0.329CysHis: 0.329 ± 0.149
0.725CysIle: 0.725 ± 0.243
0.922CysLys: 0.922 ± 0.255
0.856CysLeu: 0.856 ± 0.238
0.395CysMet: 0.395 ± 0.154
0.659CysAsn: 0.659 ± 0.231
0.264CysPro: 0.264 ± 0.121
0.198CysGln: 0.198 ± 0.132
0.725CysArg: 0.725 ± 0.31
0.527CysSer: 0.527 ± 0.175
0.725CysThr: 0.725 ± 0.22
0.856CysVal: 0.856 ± 0.226
0.395CysTrp: 0.395 ± 0.137
0.264CysTyr: 0.264 ± 0.129
0.0CysXaa: 0.0 ± 0.0
Asp
5.139AspAla: 5.139 ± 0.555
0.725AspCys: 0.725 ± 0.251
4.085AspAsp: 4.085 ± 0.603
4.612AspGlu: 4.612 ± 0.475
2.833AspPhe: 2.833 ± 0.465
6.522AspGly: 6.522 ± 0.706
1.054AspHis: 1.054 ± 0.268
3.426AspIle: 3.426 ± 0.483
4.085AspLys: 4.085 ± 0.453
4.282AspLeu: 4.282 ± 0.532
2.24AspMet: 2.24 ± 0.369
2.503AspAsn: 2.503 ± 0.392
2.306AspPro: 2.306 ± 0.352
2.108AspGln: 2.108 ± 0.328
2.635AspArg: 2.635 ± 0.377
3.558AspSer: 3.558 ± 0.466
3.36AspThr: 3.36 ± 0.46
3.294AspVal: 3.294 ± 0.47
0.922AspTrp: 0.922 ± 0.251
2.701AspTyr: 2.701 ± 0.385
0.0AspXaa: 0.0 ± 0.0
Glu
5.6GluAla: 5.6 ± 0.715
1.054GluCys: 1.054 ± 0.262
3.031GluAsp: 3.031 ± 0.393
4.414GluGlu: 4.414 ± 0.475
3.162GluPhe: 3.162 ± 0.465
3.162GluGly: 3.162 ± 0.498
1.12GluHis: 1.12 ± 0.282
5.6GluIle: 5.6 ± 0.656
4.612GluLys: 4.612 ± 0.578
4.809GluLeu: 4.809 ± 0.603
2.767GluMet: 2.767 ± 0.386
3.294GluAsn: 3.294 ± 0.459
1.845GluPro: 1.845 ± 0.35
2.833GluGln: 2.833 ± 0.508
3.031GluArg: 3.031 ± 0.524
4.216GluSer: 4.216 ± 0.5
3.755GluThr: 3.755 ± 0.644
4.48GluVal: 4.48 ± 0.497
0.725GluTrp: 0.725 ± 0.265
2.965GluTyr: 2.965 ± 0.416
0.0GluXaa: 0.0 ± 0.0
Phe
2.701PheAla: 2.701 ± 0.444
0.461PheCys: 0.461 ± 0.219
3.096PheAsp: 3.096 ± 0.456
2.108PheGlu: 2.108 ± 0.345
1.515PhePhe: 1.515 ± 0.424
3.031PheGly: 3.031 ± 0.437
0.527PheHis: 0.527 ± 0.174
2.503PheIle: 2.503 ± 0.401
3.294PheLys: 3.294 ± 0.395
2.174PheLeu: 2.174 ± 0.404
0.856PheMet: 0.856 ± 0.23
2.306PheAsn: 2.306 ± 0.367
1.515PhePro: 1.515 ± 0.316
1.713PheGln: 1.713 ± 0.404
2.174PheArg: 2.174 ± 0.382
2.042PheSer: 2.042 ± 0.349
2.701PheThr: 2.701 ± 0.369
2.635PheVal: 2.635 ± 0.484
0.856PheTrp: 0.856 ± 0.314
1.054PheTyr: 1.054 ± 0.334
0.0PheXaa: 0.0 ± 0.0
Gly
4.678GlyAla: 4.678 ± 0.76
1.449GlyCys: 1.449 ± 0.311
5.007GlyAsp: 5.007 ± 0.637
5.402GlyGlu: 5.402 ± 0.534
3.096GlyPhe: 3.096 ± 0.457
6.456GlyGly: 6.456 ± 1.103
1.252GlyHis: 1.252 ± 0.316
4.612GlyIle: 4.612 ± 0.554
6.654GlyLys: 6.654 ± 0.535
5.27GlyLeu: 5.27 ± 0.481
2.701GlyMet: 2.701 ± 0.353
3.953GlyAsn: 3.953 ± 0.435
0.395GlyPro: 0.395 ± 0.166
2.174GlyGln: 2.174 ± 0.293
2.635GlyArg: 2.635 ± 0.334
6.061GlySer: 6.061 ± 0.625
3.228GlyThr: 3.228 ± 0.466
6.193GlyVal: 6.193 ± 0.61
1.383GlyTrp: 1.383 ± 0.257
3.096GlyTyr: 3.096 ± 0.364
0.0GlyXaa: 0.0 ± 0.0
His
0.791HisAla: 0.791 ± 0.29
0.264HisCys: 0.264 ± 0.169
1.252HisAsp: 1.252 ± 0.302
0.988HisGlu: 0.988 ± 0.298
0.659HisPhe: 0.659 ± 0.188
1.713HisGly: 1.713 ± 0.411
0.461HisHis: 0.461 ± 0.232
1.054HisIle: 1.054 ± 0.277
1.515HisLys: 1.515 ± 0.371
0.922HisLeu: 0.922 ± 0.263
0.198HisMet: 0.198 ± 0.133
0.725HisAsn: 0.725 ± 0.223
0.527HisPro: 0.527 ± 0.251
0.725HisGln: 0.725 ± 0.298
0.988HisArg: 0.988 ± 0.272
0.988HisSer: 0.988 ± 0.311
1.186HisThr: 1.186 ± 0.296
1.318HisVal: 1.318 ± 0.267
0.132HisTrp: 0.132 ± 0.091
1.318HisTyr: 1.318 ± 0.373
0.0HisXaa: 0.0 ± 0.0
Ile
5.797IleAla: 5.797 ± 0.636
1.054IleCys: 1.054 ± 0.274
5.534IleAsp: 5.534 ± 0.55
4.809IleGlu: 4.809 ± 0.395
1.911IlePhe: 1.911 ± 0.337
4.941IleGly: 4.941 ± 0.568
1.383IleHis: 1.383 ± 0.356
4.282IleIle: 4.282 ± 0.459
5.205IleLys: 5.205 ± 0.636
2.965IleLeu: 2.965 ± 0.399
2.108IleMet: 2.108 ± 0.389
3.36IleAsn: 3.36 ± 0.49
2.372IlePro: 2.372 ± 0.394
2.767IleGln: 2.767 ± 0.625
3.36IleArg: 3.36 ± 0.57
3.821IleSer: 3.821 ± 0.562
4.348IleThr: 4.348 ± 0.421
4.216IleVal: 4.216 ± 0.497
0.922IleTrp: 0.922 ± 0.261
2.503IleTyr: 2.503 ± 0.457
0.0IleXaa: 0.0 ± 0.0
Lys
7.115LysAla: 7.115 ± 1.061
0.856LysCys: 0.856 ± 0.247
4.612LysAsp: 4.612 ± 0.592
5.402LysGlu: 5.402 ± 0.665
2.767LysPhe: 2.767 ± 0.405
3.887LysGly: 3.887 ± 0.469
1.581LysHis: 1.581 ± 0.393
3.887LysIle: 3.887 ± 0.486
4.678LysLys: 4.678 ± 0.686
5.336LysLeu: 5.336 ± 0.549
3.36LysMet: 3.36 ± 0.496
2.899LysAsn: 2.899 ± 0.551
3.821LysPro: 3.821 ± 0.517
3.294LysGln: 3.294 ± 0.522
4.348LysArg: 4.348 ± 0.737
3.623LysSer: 3.623 ± 0.458
4.282LysThr: 4.282 ± 0.528
5.139LysVal: 5.139 ± 0.526
0.988LysTrp: 0.988 ± 0.264
1.911LysTyr: 1.911 ± 0.426
0.0LysXaa: 0.0 ± 0.0
Leu
5.6LeuAla: 5.6 ± 0.625
0.659LeuCys: 0.659 ± 0.238
3.36LeuAsp: 3.36 ± 0.504
3.623LeuGlu: 3.623 ± 0.605
2.042LeuPhe: 2.042 ± 0.453
3.821LeuGly: 3.821 ± 0.66
1.252LeuHis: 1.252 ± 0.294
5.27LeuIle: 5.27 ± 0.639
5.205LeuLys: 5.205 ± 0.64
3.887LeuLeu: 3.887 ± 0.428
1.911LeuMet: 1.911 ± 0.369
3.36LeuAsn: 3.36 ± 0.461
2.899LeuPro: 2.899 ± 0.429
1.845LeuGln: 1.845 ± 0.385
3.755LeuArg: 3.755 ± 0.428
4.348LeuSer: 4.348 ± 0.464
4.085LeuThr: 4.085 ± 0.442
3.623LeuVal: 3.623 ± 0.445
0.988LeuTrp: 0.988 ± 0.259
2.438LeuTyr: 2.438 ± 0.351
0.0LeuXaa: 0.0 ± 0.0
Met
3.492MetAla: 3.492 ± 0.433
0.329MetCys: 0.329 ± 0.12
1.581MetAsp: 1.581 ± 0.293
1.713MetGlu: 1.713 ± 0.408
0.856MetPhe: 0.856 ± 0.246
1.12MetGly: 1.12 ± 0.283
0.725MetHis: 0.725 ± 0.238
2.569MetIle: 2.569 ± 0.365
2.767MetLys: 2.767 ± 0.464
1.845MetLeu: 1.845 ± 0.381
1.186MetMet: 1.186 ± 0.316
1.383MetAsn: 1.383 ± 0.324
1.252MetPro: 1.252 ± 0.291
1.318MetGln: 1.318 ± 0.268
1.779MetArg: 1.779 ± 0.254
2.372MetSer: 2.372 ± 0.393
1.845MetThr: 1.845 ± 0.356
2.174MetVal: 2.174 ± 0.363
0.132MetTrp: 0.132 ± 0.102
0.922MetTyr: 0.922 ± 0.218
0.0MetXaa: 0.0 ± 0.0
Asn
4.48AsnAla: 4.48 ± 0.549
0.791AsnCys: 0.791 ± 0.257
2.833AsnAsp: 2.833 ± 0.454
3.031AsnGlu: 3.031 ± 0.383
2.042AsnPhe: 2.042 ± 0.331
5.6AsnGly: 5.6 ± 0.608
0.856AsnHis: 0.856 ± 0.24
3.294AsnIle: 3.294 ± 0.423
3.096AsnLys: 3.096 ± 0.549
2.24AsnLeu: 2.24 ± 0.45
1.054AsnMet: 1.054 ± 0.291
2.833AsnAsn: 2.833 ± 0.498
1.647AsnPro: 1.647 ± 0.314
2.108AsnGln: 2.108 ± 0.399
1.713AsnArg: 1.713 ± 0.281
2.438AsnSer: 2.438 ± 0.446
1.515AsnThr: 1.515 ± 0.313
3.36AsnVal: 3.36 ± 0.382
0.791AsnTrp: 0.791 ± 0.185
1.713AsnTyr: 1.713 ± 0.404
0.0AsnXaa: 0.0 ± 0.0
Pro
2.372ProAla: 2.372 ± 0.384
0.527ProCys: 0.527 ± 0.198
2.24ProAsp: 2.24 ± 0.589
3.36ProGlu: 3.36 ± 0.598
1.647ProPhe: 1.647 ± 0.264
3.228ProGly: 3.228 ± 0.425
0.461ProHis: 0.461 ± 0.188
2.108ProIle: 2.108 ± 0.421
1.449ProLys: 1.449 ± 0.224
1.779ProLeu: 1.779 ± 0.322
0.988ProMet: 0.988 ± 0.237
1.647ProAsn: 1.647 ± 0.35
1.252ProPro: 1.252 ± 0.314
1.12ProGln: 1.12 ± 0.218
1.976ProArg: 1.976 ± 0.447
1.647ProSer: 1.647 ± 0.309
1.515ProThr: 1.515 ± 0.361
2.438ProVal: 2.438 ± 0.481
0.527ProTrp: 0.527 ± 0.191
1.515ProTyr: 1.515 ± 0.318
0.0ProXaa: 0.0 ± 0.0
Gln
2.899GlnAla: 2.899 ± 0.476
0.593GlnCys: 0.593 ± 0.156
1.713GlnAsp: 1.713 ± 0.323
1.976GlnGlu: 1.976 ± 0.317
1.515GlnPhe: 1.515 ± 0.329
2.767GlnGly: 2.767 ± 0.371
0.593GlnHis: 0.593 ± 0.202
2.833GlnIle: 2.833 ± 0.516
2.438GlnLys: 2.438 ± 0.351
2.899GlnLeu: 2.899 ± 0.54
0.922GlnMet: 0.922 ± 0.29
1.449GlnAsn: 1.449 ± 0.361
1.383GlnPro: 1.383 ± 0.305
3.228GlnGln: 3.228 ± 0.982
2.438GlnArg: 2.438 ± 0.371
2.701GlnSer: 2.701 ± 0.393
1.779GlnThr: 1.779 ± 0.467
3.426GlnVal: 3.426 ± 0.449
0.659GlnTrp: 0.659 ± 0.228
1.779GlnTyr: 1.779 ± 0.362
0.0GlnXaa: 0.0 ± 0.0
Arg
3.887ArgAla: 3.887 ± 0.553
0.725ArgCys: 0.725 ± 0.263
2.767ArgAsp: 2.767 ± 0.422
3.755ArgGlu: 3.755 ± 0.447
1.845ArgPhe: 1.845 ± 0.337
2.767ArgGly: 2.767 ± 0.394
0.527ArgHis: 0.527 ± 0.201
4.019ArgIle: 4.019 ± 0.502
4.216ArgLys: 4.216 ± 0.515
2.569ArgLeu: 2.569 ± 0.418
1.449ArgMet: 1.449 ± 0.305
2.042ArgAsn: 2.042 ± 0.456
1.911ArgPro: 1.911 ± 0.431
1.845ArgGln: 1.845 ± 0.364
3.426ArgArg: 3.426 ± 0.449
2.767ArgSer: 2.767 ± 0.486
1.713ArgThr: 1.713 ± 0.328
4.612ArgVal: 4.612 ± 0.614
0.527ArgTrp: 0.527 ± 0.179
2.635ArgTyr: 2.635 ± 0.464
0.0ArgXaa: 0.0 ± 0.0
Ser
5.27SerAla: 5.27 ± 0.607
0.593SerCys: 0.593 ± 0.204
3.755SerAsp: 3.755 ± 0.43
4.48SerGlu: 4.48 ± 0.566
2.767SerPhe: 2.767 ± 0.392
5.468SerGly: 5.468 ± 0.52
0.988SerHis: 0.988 ± 0.265
4.48SerIle: 4.48 ± 0.625
4.216SerLys: 4.216 ± 0.509
3.821SerLeu: 3.821 ± 0.404
1.054SerMet: 1.054 ± 0.268
2.372SerAsn: 2.372 ± 0.438
2.108SerPro: 2.108 ± 0.377
2.372SerGln: 2.372 ± 0.442
2.899SerArg: 2.899 ± 0.369
3.426SerSer: 3.426 ± 0.605
3.426SerThr: 3.426 ± 0.514
4.48SerVal: 4.48 ± 0.565
0.988SerTrp: 0.988 ± 0.277
2.438SerTyr: 2.438 ± 0.364
0.0SerXaa: 0.0 ± 0.0
Thr
4.743ThrAla: 4.743 ± 0.759
0.659ThrCys: 0.659 ± 0.252
3.096ThrAsp: 3.096 ± 0.402
2.767ThrGlu: 2.767 ± 0.481
2.503ThrPhe: 2.503 ± 0.366
6.127ThrGly: 6.127 ± 0.688
0.527ThrHis: 0.527 ± 0.21
3.755ThrIle: 3.755 ± 0.514
3.162ThrLys: 3.162 ± 0.63
4.085ThrLeu: 4.085 ± 0.401
1.186ThrMet: 1.186 ± 0.288
3.228ThrAsn: 3.228 ± 0.435
2.108ThrPro: 2.108 ± 0.377
2.569ThrGln: 2.569 ± 0.52
1.515ThrArg: 1.515 ± 0.374
3.492ThrSer: 3.492 ± 0.502
2.306ThrThr: 2.306 ± 0.372
3.623ThrVal: 3.623 ± 0.393
0.856ThrTrp: 0.856 ± 0.236
1.713ThrTyr: 1.713 ± 0.337
0.0ThrXaa: 0.0 ± 0.0
Val
4.546ValAla: 4.546 ± 0.622
1.054ValCys: 1.054 ± 0.269
4.809ValAsp: 4.809 ± 0.441
4.809ValGlu: 4.809 ± 0.512
3.031ValPhe: 3.031 ± 0.435
4.15ValGly: 4.15 ± 0.639
1.252ValHis: 1.252 ± 0.352
4.546ValIle: 4.546 ± 0.54
6.193ValLys: 6.193 ± 0.69
4.612ValLeu: 4.612 ± 0.481
2.438ValMet: 2.438 ± 0.377
3.623ValAsn: 3.623 ± 0.527
1.779ValPro: 1.779 ± 0.464
2.042ValGln: 2.042 ± 0.405
3.228ValArg: 3.228 ± 0.467
5.139ValSer: 5.139 ± 0.619
4.678ValThr: 4.678 ± 0.699
4.546ValVal: 4.546 ± 0.529
1.12ValTrp: 1.12 ± 0.227
1.713ValTyr: 1.713 ± 0.36
0.0ValXaa: 0.0 ± 0.0
Trp
0.922TrpAla: 0.922 ± 0.289
0.198TrpCys: 0.198 ± 0.123
0.988TrpAsp: 0.988 ± 0.212
0.593TrpGlu: 0.593 ± 0.215
0.725TrpPhe: 0.725 ± 0.287
1.318TrpGly: 1.318 ± 0.309
0.659TrpHis: 0.659 ± 0.224
1.054TrpIle: 1.054 ± 0.248
0.856TrpLys: 0.856 ± 0.205
1.252TrpLeu: 1.252 ± 0.276
0.395TrpMet: 0.395 ± 0.162
0.527TrpAsn: 0.527 ± 0.202
0.395TrpPro: 0.395 ± 0.173
0.395TrpGln: 0.395 ± 0.193
1.186TrpArg: 1.186 ± 0.275
0.988TrpSer: 0.988 ± 0.244
0.725TrpThr: 0.725 ± 0.209
0.593TrpVal: 0.593 ± 0.175
0.132TrpTrp: 0.132 ± 0.09
0.659TrpTyr: 0.659 ± 0.17
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.833TyrAla: 2.833 ± 0.575
0.527TyrCys: 0.527 ± 0.178
2.503TyrAsp: 2.503 ± 0.354
2.503TyrGlu: 2.503 ± 0.44
1.186TyrPhe: 1.186 ± 0.287
2.635TyrGly: 2.635 ± 0.326
0.988TyrHis: 0.988 ± 0.264
1.581TyrIle: 1.581 ± 0.304
3.031TyrLys: 3.031 ± 0.503
1.976TyrLeu: 1.976 ± 0.361
1.186TyrMet: 1.186 ± 0.3
1.383TyrAsn: 1.383 ± 0.299
1.911TyrPro: 1.911 ± 0.331
2.108TyrGln: 2.108 ± 0.314
2.042TyrArg: 2.042 ± 0.402
2.306TyrSer: 2.306 ± 0.4
2.701TyrThr: 2.701 ± 0.351
2.306TyrVal: 2.306 ± 0.326
0.329TyrTrp: 0.329 ± 0.123
1.186TyrTyr: 1.186 ± 0.298
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (15180 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski