Thauera sp. (strain MZ1T)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae; Thauera; unclassified Thauera

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3927 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4ZJM1|C4ZJM1_THASP Aldo/keto reductase OS=Thauera sp. (strain MZ1T) OX=85643 GN=Tmz1t_1648 PE=4 SV=1
MM1 pKa = 7.41KK2 pKa = 10.45AKK4 pKa = 10.29NLVVAMLAALGASSASAALVNLNGLGYY31 pKa = 8.69VTYY34 pKa = 10.9GDD36 pKa = 3.95GNSYY40 pKa = 10.98SLAVANYY47 pKa = 7.04FACGSEE53 pKa = 4.06YY54 pKa = 11.14GPGCPYY60 pKa = 10.36NVKK63 pKa = 10.19GSPGASEE70 pKa = 4.22TDD72 pKa = 3.1PRR74 pKa = 11.84IYY76 pKa = 10.08IVQGAGGHH84 pKa = 5.59VDD86 pKa = 3.66NEE88 pKa = 4.08NANIDD93 pKa = 4.04DD94 pKa = 5.19AFDD97 pKa = 3.63AANNEE102 pKa = 4.28LFFTMTSANEE112 pKa = 4.02PGSDD116 pKa = 3.45GNKK119 pKa = 9.66NATFTGDD126 pKa = 3.87KK127 pKa = 10.14TNTWDD132 pKa = 3.45ATLGALDD139 pKa = 3.81QSFDD143 pKa = 3.5FSKK146 pKa = 11.44NLMTFFFVNNEE157 pKa = 4.02PNSNDD162 pKa = 3.4GLTQNLAVWARR173 pKa = 11.84VTLTNLAGTTVYY185 pKa = 9.21KK186 pKa = 10.62TFDD189 pKa = 3.39VTNDD193 pKa = 2.97PRR195 pKa = 11.84TSEE198 pKa = 3.89QRR200 pKa = 11.84DD201 pKa = 3.21GDD203 pKa = 4.38GIGDD207 pKa = 4.6LPAPGYY213 pKa = 10.25GPPPVGGGVPYY224 pKa = 11.06GDD226 pKa = 3.23VTLYY230 pKa = 10.39TSEE233 pKa = 4.36SNAPEE238 pKa = 3.75ITDD241 pKa = 3.75FAMSGGQVCVDD252 pKa = 3.97EE253 pKa = 4.4NTSTLVDD260 pKa = 3.98CTTASAGSFDD270 pKa = 5.76AINHH274 pKa = 5.91NLGEE278 pKa = 4.16NQAPYY283 pKa = 10.64AITIPEE289 pKa = 4.46LDD291 pKa = 4.33DD292 pKa = 4.26YY293 pKa = 11.59IGGLMAANANLDD305 pKa = 4.05DD306 pKa = 3.76YY307 pKa = 11.78VMRR310 pKa = 11.84VDD312 pKa = 4.68LRR314 pKa = 11.84YY315 pKa = 10.56GCVSPPFTIDD325 pKa = 3.02TQGNDD330 pKa = 2.86SCVGYY335 pKa = 8.41GTAVSQDD342 pKa = 3.26NNFEE346 pKa = 4.13QVFISTRR353 pKa = 11.84LATQRR358 pKa = 11.84VPEE361 pKa = 4.8PGTLALLGSGLLGFAAARR379 pKa = 11.84RR380 pKa = 11.84SRR382 pKa = 11.84KK383 pKa = 9.09SS384 pKa = 2.98

Molecular weight: 40.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4ZJF4|C4ZJF4_THASP Uncharacterized protein OS=Thauera sp. (strain MZ1T) OX=85643 GN=Tmz1t_1128 PE=4 SV=1
MM1 pKa = 7.22NGKK4 pKa = 8.97HH5 pKa = 6.13PAQGTHH11 pKa = 5.76SPAIGAGRR19 pKa = 11.84RR20 pKa = 11.84SLQQRR25 pKa = 11.84LLALRR30 pKa = 11.84AGLRR34 pKa = 11.84AALRR38 pKa = 11.84AFRR41 pKa = 11.84THH43 pKa = 7.53PIKK46 pKa = 10.75HH47 pKa = 5.49PQRR50 pKa = 5.12

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3927

0

3927

1335952

31

5342

340.2

36.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.347 ± 0.06

0.979 ± 0.016

5.456 ± 0.028

6.061 ± 0.037

3.558 ± 0.021

8.474 ± 0.037

2.307 ± 0.02

4.413 ± 0.026

2.829 ± 0.03

11.136 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.282 ± 0.018

2.284 ± 0.021

5.207 ± 0.03

3.233 ± 0.022

7.966 ± 0.045

4.794 ± 0.03

4.631 ± 0.026

7.516 ± 0.034

1.411 ± 0.016

2.117 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski