Flavobacterium branchiophilum (strain FL-15)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; Flavobacterium branchiophilum

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2848 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2Z5M4|G2Z5M4_FLABF Uncharacterized protein OS=Flavobacterium branchiophilum (strain FL-15) OX=1034807 GN=FBFL15_2851 PE=4 SV=1
MM1 pKa = 8.4DD2 pKa = 3.69YY3 pKa = 10.49TDD5 pKa = 6.39KK6 pKa = 11.1IFLWLGIFNGSKK18 pKa = 10.84NEE20 pKa = 3.71FNLYY24 pKa = 9.86FDD26 pKa = 3.38QSKK29 pKa = 10.91GYY31 pKa = 10.75LLDD34 pKa = 4.13EE35 pKa = 4.75YY36 pKa = 11.53GNDD39 pKa = 3.54RR40 pKa = 11.84LPEE43 pKa = 4.52EE44 pKa = 4.62IEE46 pKa = 3.94LSQFSKK52 pKa = 11.24DD53 pKa = 3.2LGEE56 pKa = 4.71DD57 pKa = 3.26FTYY60 pKa = 11.03DD61 pKa = 3.38EE62 pKa = 5.38DD63 pKa = 4.97FIGYY67 pKa = 9.54LLFPNSIEE75 pKa = 3.63ILEE78 pKa = 4.25ILEE81 pKa = 4.13EE82 pKa = 4.23VPINHH87 pKa = 5.95SEE89 pKa = 4.01KK90 pKa = 10.3EE91 pKa = 4.34SVIKK95 pKa = 10.56KK96 pKa = 10.25CIEE99 pKa = 4.27LGLNDD104 pKa = 3.77ANAVYY109 pKa = 9.35WYY111 pKa = 10.36SDD113 pKa = 3.91EE114 pKa = 4.09ITPNPNISYY123 pKa = 10.7NGLKK127 pKa = 10.77YY128 pKa = 10.13IGEE131 pKa = 4.37YY132 pKa = 10.11LAEE135 pKa = 4.18

Molecular weight: 15.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2Z6V9|G2Z6V9_FLABF Uncharacterized protein OS=Flavobacterium branchiophilum (strain FL-15) OX=1034807 GN=FBFL15_0850 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.91HH17 pKa = 4.39GFMDD21 pKa = 4.5RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.41KK42 pKa = 10.14LTVSSEE48 pKa = 3.92PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2848

0

2848

975703

33

3174

342.6

38.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.339 ± 0.047

0.858 ± 0.015

5.144 ± 0.029

5.974 ± 0.054

5.539 ± 0.043

5.921 ± 0.048

1.895 ± 0.025

8.56 ± 0.055

8.136 ± 0.057

9.213 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.207 ± 0.021

6.872 ± 0.059

3.305 ± 0.023

4.039 ± 0.028

2.835 ± 0.025

6.164 ± 0.044

5.965 ± 0.072

5.833 ± 0.036

1.036 ± 0.018

4.164 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski