Dechloromonas denitrificans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Azonexaceae; Dechloromonas

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3886 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133XM53|A0A133XM53_9RHOO OmpA-like domain-containing protein OS=Dechloromonas denitrificans OX=281362 GN=AT959_02895 PE=4 SV=1
MM1 pKa = 7.2NAVTEE6 pKa = 4.36MTSPLLFTDD15 pKa = 3.68NAANKK20 pKa = 9.13VKK22 pKa = 10.39EE23 pKa = 4.28LIEE26 pKa = 4.21EE27 pKa = 4.26EE28 pKa = 4.49GNPGLKK34 pKa = 10.26LRR36 pKa = 11.84VFVTGGGCSGFQYY49 pKa = 10.93GFTFDD54 pKa = 4.28EE55 pKa = 4.93EE56 pKa = 4.59VNEE59 pKa = 5.46DD60 pKa = 3.7DD61 pKa = 3.86TTMEE65 pKa = 4.34KK66 pKa = 10.84NGVTLLIDD74 pKa = 3.7PMSYY78 pKa = 10.39QYY80 pKa = 11.8LLGAEE85 pKa = 4.0IDD87 pKa = 3.8YY88 pKa = 11.47SEE90 pKa = 4.55GLEE93 pKa = 4.01GSQFVIRR100 pKa = 11.84NPNATSTCGCGSSFSAA116 pKa = 4.79

Molecular weight: 12.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133XG86|A0A133XG86_9RHOO ABC transporter ATP-binding protein OS=Dechloromonas denitrificans OX=281362 GN=AT959_17580 PE=4 SV=1
MM1 pKa = 6.98STLVLPPAIRR11 pKa = 11.84SLAHH15 pKa = 6.26RR16 pKa = 11.84NFRR19 pKa = 11.84LYY21 pKa = 10.87FLGQAISILGSWIQQVALSWLVYY44 pKa = 10.43RR45 pKa = 11.84LTGSAALLGVTAFCALIPQLLVGPLAGAWIDD76 pKa = 3.69KK77 pKa = 9.32QDD79 pKa = 3.3KK80 pKa = 9.78RR81 pKa = 11.84KK82 pKa = 9.0WLIGIQSLLAAQAFLLAGLTWSGLIGTGFIILMSLTLGILNSFDD126 pKa = 3.42TPLRR130 pKa = 11.84QSLIGSFVGSRR141 pKa = 11.84EE142 pKa = 4.05DD143 pKa = 4.23LPNALALNAMLFNAGRR159 pKa = 11.84FVGPPIAGLLVGLTSEE175 pKa = 4.53AACFAINGFSFLALIGGLRR194 pKa = 11.84FIQMTASPRR203 pKa = 11.84ASGSVGQVFKK213 pKa = 11.28EE214 pKa = 4.23GVLYY218 pKa = 10.35AWQTWTVRR226 pKa = 11.84MLIITLIGLNLTASAYY242 pKa = 10.35AVLLPVFARR251 pKa = 11.84DD252 pKa = 3.56VFAGDD257 pKa = 3.33ATTLGWLWGAAGCGAFASTIFLATRR282 pKa = 11.84KK283 pKa = 7.56TIPGLITAVVAGVAISAVALLLFGATTRR311 pKa = 11.84LPLALTAMVALGFGISVCNVGINMILQSAAPDD343 pKa = 3.48QLRR346 pKa = 11.84GRR348 pKa = 11.84IVSFFTSARR357 pKa = 11.84FGFDD361 pKa = 2.95ALGGLIAGFVAGALGAGQTLLFEE384 pKa = 4.71GVALFLFVIFLLTRR398 pKa = 11.84RR399 pKa = 11.84HH400 pKa = 6.33RR401 pKa = 11.84LTAQVSAAHH410 pKa = 6.48GEE412 pKa = 4.36SSNGHH417 pKa = 5.54

Molecular weight: 43.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3886

0

3886

1254878

55

3437

322.9

35.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.948 ± 0.06

0.993 ± 0.014

5.237 ± 0.034

6.001 ± 0.038

3.734 ± 0.027

8.073 ± 0.043

2.193 ± 0.018

5.161 ± 0.029

3.9 ± 0.035

11.229 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.02

2.994 ± 0.029

4.85 ± 0.03

3.919 ± 0.026

6.428 ± 0.043

5.437 ± 0.032

4.771 ± 0.033

7.035 ± 0.033

1.353 ± 0.018

2.346 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski