Tenacibaculum soleae
Average proteome isoelectric point is 7.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2722 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B9XZA5|A0A1B9XZA5_9FLAO Two-component system response regulator OS=Tenacibaculum soleae OX=447689 GN=BA195_08175 PE=4 SV=1
MM1 pKa = 7.4 KK2 pKa = 10.2 KK3 pKa = 10.62 VNLFMIALALAFTTQKK19 pKa = 9.52 TVAQDD24 pKa = 3.72 NNDD27 pKa = 3.63 TNQATHH33 pKa = 6.05 QLDD36 pKa = 3.86 VNVPEE41 pKa = 5.13 LALIDD46 pKa = 3.19 IWDD49 pKa = 4.01 ANEE52 pKa = 3.81 GAEE55 pKa = 4.33 AATILMDD62 pKa = 3.7 MANVTLVGTNAEE74 pKa = 4.01 AGLYY78 pKa = 10.07 AFQDD82 pKa = 2.99 MDD84 pKa = 3.86 YY85 pKa = 11.38 QNLYY89 pKa = 10.86 LNYY92 pKa = 9.76 TSVTGAGGSGFDD104 pKa = 3.33 VTRR107 pKa = 11.84 QIDD110 pKa = 3.62 VQFEE114 pKa = 4.09 AGSTFPGSLDD124 pKa = 3.88 LRR126 pKa = 11.84 ITPEE130 pKa = 3.89 APLVVVNGGTVDD142 pKa = 3.2 SAGTVTAGGVALGATTPIGTDD163 pKa = 3.09 VLLVNSIEE171 pKa = 4.26 SVYY174 pKa = 10.89 TGDD177 pKa = 3.21 EE178 pKa = 4.19 TQGVKK183 pKa = 10.06 LAYY186 pKa = 9.3 TLEE189 pKa = 4.26 QNGNFAGYY197 pKa = 9.32 QAGSYY202 pKa = 9.82 SAVIRR207 pKa = 11.84 YY208 pKa = 6.96 TLSDD212 pKa = 3.25 NN213 pKa = 3.82
Molecular weight: 22.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.77
IPC_protein 3.757
Toseland 3.541
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.579
Grimsley 3.452
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 4.101
Thurlkill 3.605
EMBOSS 3.706
Sillero 3.872
Patrickios 0.947
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.779
Protein with the highest isoelectric point:
>tr|A0A1B9XWN2|A0A1B9XWN2_9FLAO Mechanosensitive ion channel protein MscS OS=Tenacibaculum soleae OX=447689 GN=BA195_13110 PE=3 SV=1
MM1 pKa = 8.03 PKK3 pKa = 8.97 RR4 pKa = 11.84 TYY6 pKa = 10.33 QPSKK10 pKa = 9.01 RR11 pKa = 11.84 KK12 pKa = 9.47 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.27 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.8 GRR40 pKa = 11.84 KK41 pKa = 8.71 SISVSSAPRR50 pKa = 11.84 PKK52 pKa = 10.38 KK53 pKa = 10.5
Molecular weight: 6.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.359
IPC2_protein 10.921
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.281
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.003
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.043
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2722
0
2722
891548
50
3923
327.5
37.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.066 ± 0.044
0.697 ± 0.013
5.186 ± 0.031
6.389 ± 0.048
5.335 ± 0.036
6.003 ± 0.046
1.699 ± 0.02
8.471 ± 0.048
9.278 ± 0.058
9.184 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.062 ± 0.028
6.841 ± 0.057
3.036 ± 0.025
3.298 ± 0.027
3.09 ± 0.029
6.256 ± 0.043
6.017 ± 0.05
6.156 ± 0.039
0.956 ± 0.019
3.98 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here