Streptomyces sp. DvalAA-43

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8159 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C4MUD1|A0A1C4MUD1_9ACTN Proteasome accessory factor C OS=Streptomyces sp. DvalAA-43 OX=1839762 GN=GA0115234_1052196 PE=4 SV=1
MM1 pKa = 6.99QQDD4 pKa = 3.75APNGGDD10 pKa = 3.57TQDD13 pKa = 3.64LEE15 pKa = 4.23VWIDD19 pKa = 3.38QDD21 pKa = 3.99LCTGDD26 pKa = 4.65GICVQYY32 pKa = 10.96APEE35 pKa = 4.18VFEE38 pKa = 5.9LDD40 pKa = 3.05IDD42 pKa = 3.63GLAYY46 pKa = 10.42VKK48 pKa = 10.8SADD51 pKa = 5.3DD52 pKa = 4.73EE53 pKa = 4.41LLQDD57 pKa = 4.41KK58 pKa = 10.51GATTPVPLPLLQDD71 pKa = 3.66VVDD74 pKa = 4.35SAKK77 pKa = 10.25EE78 pKa = 3.92CPGDD82 pKa = 4.18CIHH85 pKa = 6.28VRR87 pKa = 11.84RR88 pKa = 11.84VSDD91 pKa = 3.47SVEE94 pKa = 4.1VYY96 pKa = 10.75GPEE99 pKa = 3.83AAA101 pKa = 4.91

Molecular weight: 10.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C4SSD9|A0A1C4SSD9_9ACTN CubicO group peptidase beta-lactamase class C family OS=Streptomyces sp. DvalAA-43 OX=1839762 GN=GA0115234_1094486 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.47GRR40 pKa = 11.84ANLSAA45 pKa = 4.66

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8159

0

8159

2715702

26

6604

332.8

35.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.465 ± 0.042

0.802 ± 0.008

6.036 ± 0.021

5.615 ± 0.027

2.716 ± 0.016

9.562 ± 0.026

2.335 ± 0.014

3.251 ± 0.019

2.135 ± 0.023

10.253 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.758 ± 0.012

1.771 ± 0.013

6.207 ± 0.025

2.718 ± 0.016

8.121 ± 0.03

5.163 ± 0.019

6.23 ± 0.023

8.296 ± 0.024

1.503 ± 0.011

2.062 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski