Acinetobacter phage vB_AbaM_phiAbaA1
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 165 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A126DI51|A0A126DI51_9CAUD Terminase large subunit OS=Acinetobacter phage vB_AbaM_phiAbaA1 OX=1605379 GN=phiAbaA1_027 PE=4 SV=1
MM1 pKa = 7.86 EE2 pKa = 6.49 DD3 pKa = 5.4 DD4 pKa = 3.8 EE5 pKa = 5.82 DD6 pKa = 3.78 LTIVVVAIIAMIIYY20 pKa = 10.15 IGIAYY25 pKa = 8.65 FFLKK29 pKa = 7.89 TTMGAFVLYY38 pKa = 10.65 SLFGVILL45 pKa = 3.86
Molecular weight: 5.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.879
IPC2_protein 3.834
IPC_protein 3.528
Toseland 3.401
ProMoST 3.808
Dawson 3.579
Bjellqvist 3.77
Wikipedia 3.605
Rodwell 3.427
Grimsley 3.338
Solomon 3.478
Lehninger 3.427
Nozaki 3.783
DTASelect 3.872
Thurlkill 3.528
EMBOSS 3.605
Sillero 3.681
Patrickios 0.299
IPC_peptide 3.465
IPC2_peptide 3.63
IPC2.peptide.svr19 3.714
Protein with the highest isoelectric point:
>tr|A0A126DIU3|A0A126DIU3_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaM_phiAbaA1 OX=1605379 GN=phiAbaA1_075 PE=4 SV=1
MM1 pKa = 7.65 VITLIKK7 pKa = 10.05 IIFIVFYY14 pKa = 8.85 FINVACEE21 pKa = 3.46 VSNRR25 pKa = 11.84 YY26 pKa = 8.93 RR27 pKa = 11.84 DD28 pKa = 3.89 CKK30 pKa = 8.68 MHH32 pKa = 5.48 VAVRR36 pKa = 11.84 YY37 pKa = 7.2 TFMVASAIAYY47 pKa = 6.69 TKK49 pKa = 10.38 IGVLATAPLVVQISALIIVIFLAKK73 pKa = 10.28 FLYY76 pKa = 10.63 DD77 pKa = 3.48 HH78 pKa = 5.86 VLKK81 pKa = 10.77 RR82 pKa = 11.84 QIRR85 pKa = 11.84 IKK87 pKa = 10.75 NFRR90 pKa = 11.84 LKK92 pKa = 10.46 KK93 pKa = 10.36 LYY95 pKa = 10.49 LSEE98 pKa = 4.32 SARR101 pKa = 11.84 KK102 pKa = 8.78 PSHH105 pKa = 5.9 KK106 pKa = 10.31 LNRR109 pKa = 11.84 RR110 pKa = 3.35
Molecular weight: 12.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 9.999
IPC_protein 10.526
Toseland 10.643
ProMoST 10.423
Dawson 10.774
Bjellqvist 10.452
Wikipedia 10.95
Rodwell 11.213
Grimsley 10.833
Solomon 10.833
Lehninger 10.804
Nozaki 10.628
DTASelect 10.452
Thurlkill 10.657
EMBOSS 11.038
Sillero 10.687
Patrickios 10.935
IPC_peptide 10.833
IPC2_peptide 9.355
IPC2.peptide.svr19 8.398
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
165
0
165
31583
31
3259
191.4
21.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.529 ± 0.395
1.172 ± 0.122
6.519 ± 0.16
7.023 ± 0.327
4.341 ± 0.173
6.766 ± 0.19
1.824 ± 0.154
6.659 ± 0.173
7.855 ± 0.27
7.862 ± 0.193
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.672 ± 0.127
5.18 ± 0.183
2.843 ± 0.116
3.35 ± 0.175
4.392 ± 0.178
6.323 ± 0.179
5.965 ± 0.373
7.21 ± 0.211
1.374 ± 0.07
4.141 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here