Paenibacillus phage Harrison

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gochnauervirinae; Harrisonvirus; Paenibacillus virus Harrison

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2CY90|A0A0K2CY90_9CAUD Uncharacterized protein OS=Paenibacillus phage Harrison OX=1636257 GN=HARRISON_71 PE=4 SV=1
MM1 pKa = 7.43TGQGIYY7 pKa = 10.31DD8 pKa = 4.56LYY10 pKa = 9.59MSVYY14 pKa = 9.47EE15 pKa = 4.82KK16 pKa = 11.28YY17 pKa = 10.8LFSEE21 pKa = 5.04DD22 pKa = 3.53PAEE25 pKa = 4.07VEE27 pKa = 4.1MLHH30 pKa = 7.02EE31 pKa = 4.56EE32 pKa = 3.97LQEE35 pKa = 3.65IRR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 10.49YY40 pKa = 10.32GIPDD44 pKa = 3.35AQQ46 pKa = 3.28

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2CYL2|A0A0K2CYL2_9CAUD Uncharacterized protein OS=Paenibacillus phage Harrison OX=1636257 GN=HARRISON_51 PE=4 SV=1
MM1 pKa = 6.97KK2 pKa = 10.21RR3 pKa = 11.84KK4 pKa = 9.54YY5 pKa = 10.53LRR7 pKa = 11.84ACRR10 pKa = 11.84EE11 pKa = 4.12SLGLPRR17 pKa = 11.84PAAANKK23 pKa = 10.27LGISQIHH30 pKa = 5.73LRR32 pKa = 11.84KK33 pKa = 10.31LEE35 pKa = 4.67DD36 pKa = 3.76GSVNPSVLVLQRR48 pKa = 11.84FCEE51 pKa = 4.48LYY53 pKa = 10.74GKK55 pKa = 9.67NPQEE59 pKa = 4.1LFPDD63 pKa = 3.67IFRR66 pKa = 11.84YY67 pKa = 5.45TTSRR71 pKa = 11.84QRR73 pKa = 3.2

Molecular weight: 8.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

13720

30

1234

163.3

18.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.246 ± 0.551

0.955 ± 0.139

5.809 ± 0.298

7.85 ± 0.406

4.111 ± 0.213

6.166 ± 0.246

1.735 ± 0.181

7.296 ± 0.32

8.331 ± 0.304

8.652 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.726 ± 0.198

5.168 ± 0.272

3.389 ± 0.18

4.031 ± 0.144

4.964 ± 0.307

5.999 ± 0.284

5.343 ± 0.202

6.297 ± 0.267

1.363 ± 0.122

3.571 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski