Amino acid dipepetide frequency for Paenibacillus phage Harrison

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.009AlaAla: 4.009 ± 0.64
0.875AlaCys: 0.875 ± 0.269
3.426AlaAsp: 3.426 ± 0.57
5.03AlaGlu: 5.03 ± 0.643
2.405AlaPhe: 2.405 ± 0.524
4.373AlaGly: 4.373 ± 0.65
1.093AlaHis: 1.093 ± 0.27
4.373AlaIle: 4.373 ± 0.671
4.738AlaLys: 4.738 ± 0.768
6.779AlaLeu: 6.779 ± 1.4
1.677AlaMet: 1.677 ± 0.277
2.624AlaAsn: 2.624 ± 0.407
1.239AlaPro: 1.239 ± 0.285
2.697AlaGln: 2.697 ± 0.434
2.624AlaArg: 2.624 ± 0.517
3.426AlaSer: 3.426 ± 0.603
3.134AlaThr: 3.134 ± 0.633
4.519AlaVal: 4.519 ± 0.939
0.875AlaTrp: 0.875 ± 0.265
2.551AlaTyr: 2.551 ± 0.453
0.0AlaXaa: 0.0 ± 0.0
Cys
0.292CysAla: 0.292 ± 0.148
0.146CysCys: 0.146 ± 0.1
0.656CysAsp: 0.656 ± 0.189
1.02CysGlu: 1.02 ± 0.24
0.292CysPhe: 0.292 ± 0.141
0.875CysGly: 0.875 ± 0.286
0.364CysHis: 0.364 ± 0.189
0.51CysIle: 0.51 ± 0.196
0.656CysLys: 0.656 ± 0.25
0.875CysLeu: 0.875 ± 0.217
0.073CysMet: 0.073 ± 0.067
0.437CysAsn: 0.437 ± 0.152
0.51CysPro: 0.51 ± 0.21
0.292CysGln: 0.292 ± 0.131
0.583CysArg: 0.583 ± 0.227
0.364CysSer: 0.364 ± 0.155
0.437CysThr: 0.437 ± 0.193
0.51CysVal: 0.51 ± 0.271
0.146CysTrp: 0.146 ± 0.103
0.51CysTyr: 0.51 ± 0.177
0.0CysXaa: 0.0 ± 0.0
Asp
3.426AspAla: 3.426 ± 0.548
0.437AspCys: 0.437 ± 0.178
4.446AspAsp: 4.446 ± 0.634
5.54AspGlu: 5.54 ± 0.665
2.551AspPhe: 2.551 ± 0.344
5.175AspGly: 5.175 ± 0.869
1.239AspHis: 1.239 ± 0.301
3.645AspIle: 3.645 ± 0.565
3.207AspLys: 3.207 ± 0.619
4.884AspLeu: 4.884 ± 0.688
2.041AspMet: 2.041 ± 0.322
2.405AspAsn: 2.405 ± 0.653
1.895AspPro: 1.895 ± 0.449
2.333AspGln: 2.333 ± 0.494
2.551AspArg: 2.551 ± 0.453
2.77AspSer: 2.77 ± 0.614
2.843AspThr: 2.843 ± 0.499
3.936AspVal: 3.936 ± 0.578
0.948AspTrp: 0.948 ± 0.228
1.822AspTyr: 1.822 ± 0.337
0.0AspXaa: 0.0 ± 0.0
Glu
4.665GluAla: 4.665 ± 0.599
0.583GluCys: 0.583 ± 0.229
4.884GluAsp: 4.884 ± 0.645
5.831GluGlu: 5.831 ± 0.839
3.061GluPhe: 3.061 ± 0.365
3.79GluGly: 3.79 ± 0.458
0.802GluHis: 0.802 ± 0.243
5.758GluIle: 5.758 ± 0.593
6.56GluLys: 6.56 ± 0.735
7.799GluLeu: 7.799 ± 0.997
2.551GluMet: 2.551 ± 0.473
4.301GluAsn: 4.301 ± 0.625
2.187GluPro: 2.187 ± 0.54
4.592GluGln: 4.592 ± 0.66
4.301GluArg: 4.301 ± 0.578
3.717GluSer: 3.717 ± 0.609
4.155GluThr: 4.155 ± 0.526
5.175GluVal: 5.175 ± 0.796
0.729GluTrp: 0.729 ± 0.241
3.061GluTyr: 3.061 ± 0.519
0.0GluXaa: 0.0 ± 0.0
Phe
2.478PheAla: 2.478 ± 0.616
0.948PheCys: 0.948 ± 0.316
2.916PheAsp: 2.916 ± 0.505
2.624PheGlu: 2.624 ± 0.401
1.458PhePhe: 1.458 ± 0.374
2.551PheGly: 2.551 ± 0.427
1.02PheHis: 1.02 ± 0.282
2.989PheIle: 2.989 ± 0.476
2.843PheLys: 2.843 ± 0.439
3.353PheLeu: 3.353 ± 0.448
0.875PheMet: 0.875 ± 0.283
2.114PheAsn: 2.114 ± 0.372
1.385PhePro: 1.385 ± 0.298
1.531PheGln: 1.531 ± 0.421
1.093PheArg: 1.093 ± 0.321
3.499PheSer: 3.499 ± 0.686
2.697PheThr: 2.697 ± 0.476
2.843PheVal: 2.843 ± 0.513
0.51PheTrp: 0.51 ± 0.197
1.385PheTyr: 1.385 ± 0.315
0.0PheXaa: 0.0 ± 0.0
Gly
3.353GlyAla: 3.353 ± 0.695
0.802GlyCys: 0.802 ± 0.217
4.082GlyAsp: 4.082 ± 0.604
5.175GlyGlu: 5.175 ± 0.804
2.114GlyPhe: 2.114 ± 0.46
4.373GlyGly: 4.373 ± 0.682
0.729GlyHis: 0.729 ± 0.209
5.758GlyIle: 5.758 ± 0.98
5.904GlyLys: 5.904 ± 0.591
4.738GlyLeu: 4.738 ± 0.559
1.604GlyMet: 1.604 ± 0.311
3.717GlyAsn: 3.717 ± 0.492
1.02GlyPro: 1.02 ± 0.272
2.405GlyGln: 2.405 ± 0.403
3.134GlyArg: 3.134 ± 0.608
2.77GlySer: 2.77 ± 0.532
2.916GlyThr: 2.916 ± 0.519
3.936GlyVal: 3.936 ± 0.61
0.802GlyTrp: 0.802 ± 0.194
2.333GlyTyr: 2.333 ± 0.408
0.0GlyXaa: 0.0 ± 0.0
His
0.948HisAla: 0.948 ± 0.238
0.364HisCys: 0.364 ± 0.167
0.51HisAsp: 0.51 ± 0.176
1.239HisGlu: 1.239 ± 0.398
1.02HisPhe: 1.02 ± 0.236
1.02HisGly: 1.02 ± 0.345
0.364HisHis: 0.364 ± 0.187
0.948HisIle: 0.948 ± 0.273
0.875HisLys: 0.875 ± 0.265
2.697HisLeu: 2.697 ± 0.44
0.656HisMet: 0.656 ± 0.211
0.948HisAsn: 0.948 ± 0.23
0.729HisPro: 0.729 ± 0.288
0.437HisGln: 0.437 ± 0.173
0.875HisArg: 0.875 ± 0.231
0.802HisSer: 0.802 ± 0.281
0.729HisThr: 0.729 ± 0.241
1.385HisVal: 1.385 ± 0.248
0.364HisTrp: 0.364 ± 0.152
0.437HisTyr: 0.437 ± 0.179
0.0HisXaa: 0.0 ± 0.0
Ile
4.228IleAla: 4.228 ± 1.06
0.729IleCys: 0.729 ± 0.261
3.936IleAsp: 3.936 ± 0.687
6.487IleGlu: 6.487 ± 0.633
2.989IlePhe: 2.989 ± 0.486
4.228IleGly: 4.228 ± 0.675
1.166IleHis: 1.166 ± 0.336
4.738IleIle: 4.738 ± 0.793
7.727IleLys: 7.727 ± 0.82
5.758IleLeu: 5.758 ± 0.701
1.749IleMet: 1.749 ± 0.44
3.79IleAsn: 3.79 ± 0.509
2.843IlePro: 2.843 ± 0.486
3.645IleGln: 3.645 ± 0.511
3.717IleArg: 3.717 ± 0.48
4.738IleSer: 4.738 ± 0.606
4.009IleThr: 4.009 ± 0.44
3.936IleVal: 3.936 ± 0.565
0.729IleTrp: 0.729 ± 0.227
1.822IleTyr: 1.822 ± 0.331
0.0IleXaa: 0.0 ± 0.0
Lys
6.05LysAla: 6.05 ± 0.82
0.364LysCys: 0.364 ± 0.234
4.592LysAsp: 4.592 ± 0.667
6.706LysGlu: 6.706 ± 0.711
2.478LysPhe: 2.478 ± 0.422
5.175LysGly: 5.175 ± 0.602
1.458LysHis: 1.458 ± 0.31
5.54LysIle: 5.54 ± 0.586
6.852LysLys: 6.852 ± 0.969
7.143LysLeu: 7.143 ± 0.632
2.624LysMet: 2.624 ± 0.435
4.665LysAsn: 4.665 ± 0.508
1.968LysPro: 1.968 ± 0.43
3.572LysGln: 3.572 ± 0.565
5.394LysArg: 5.394 ± 0.683
4.446LysSer: 4.446 ± 0.508
5.467LysThr: 5.467 ± 0.608
4.811LysVal: 4.811 ± 0.609
1.312LysTrp: 1.312 ± 0.327
2.697LysTyr: 2.697 ± 0.64
0.0LysXaa: 0.0 ± 0.0
Leu
5.831LeuAla: 5.831 ± 0.798
0.729LeuCys: 0.729 ± 0.204
5.977LeuAsp: 5.977 ± 0.616
6.633LeuGlu: 6.633 ± 0.66
4.228LeuPhe: 4.228 ± 0.586
5.102LeuGly: 5.102 ± 0.887
1.385LeuHis: 1.385 ± 0.284
6.56LeuIle: 6.56 ± 0.81
6.779LeuLys: 6.779 ± 0.749
6.05LeuLeu: 6.05 ± 0.668
2.26LeuMet: 2.26 ± 0.377
4.665LeuAsn: 4.665 ± 0.594
3.936LeuPro: 3.936 ± 0.452
3.936LeuGln: 3.936 ± 0.544
4.446LeuArg: 4.446 ± 0.592
5.467LeuSer: 5.467 ± 0.641
4.738LeuThr: 4.738 ± 0.578
4.009LeuVal: 4.009 ± 0.458
0.729LeuTrp: 0.729 ± 0.209
3.061LeuTyr: 3.061 ± 0.456
0.0LeuXaa: 0.0 ± 0.0
Met
2.114MetAla: 2.114 ± 0.466
0.146MetCys: 0.146 ± 0.1
1.312MetAsp: 1.312 ± 0.342
1.895MetGlu: 1.895 ± 0.327
1.458MetPhe: 1.458 ± 0.272
1.458MetGly: 1.458 ± 0.3
0.219MetHis: 0.219 ± 0.121
1.968MetIle: 1.968 ± 0.43
2.916MetLys: 2.916 ± 0.434
2.26MetLeu: 2.26 ± 0.365
0.729MetMet: 0.729 ± 0.217
2.041MetAsn: 2.041 ± 0.427
0.729MetPro: 0.729 ± 0.242
1.093MetGln: 1.093 ± 0.266
1.166MetArg: 1.166 ± 0.346
1.822MetSer: 1.822 ± 0.332
1.458MetThr: 1.458 ± 0.318
0.875MetVal: 0.875 ± 0.218
0.583MetTrp: 0.583 ± 0.205
1.02MetTyr: 1.02 ± 0.29
0.0MetXaa: 0.0 ± 0.0
Asn
3.717AsnAla: 3.717 ± 0.656
0.292AsnCys: 0.292 ± 0.124
2.26AsnAsp: 2.26 ± 0.45
3.717AsnGlu: 3.717 ± 0.558
1.531AsnPhe: 1.531 ± 0.373
3.645AsnGly: 3.645 ± 0.65
1.312AsnHis: 1.312 ± 0.358
2.916AsnIle: 2.916 ± 0.501
4.155AsnLys: 4.155 ± 0.607
4.519AsnLeu: 4.519 ± 0.524
1.458AsnMet: 1.458 ± 0.452
2.77AsnAsn: 2.77 ± 0.6
2.333AsnPro: 2.333 ± 0.493
2.916AsnGln: 2.916 ± 0.685
2.697AsnArg: 2.697 ± 0.418
3.645AsnSer: 3.645 ± 0.531
2.187AsnThr: 2.187 ± 0.344
2.916AsnVal: 2.916 ± 0.432
0.583AsnTrp: 0.583 ± 0.2
2.114AsnTyr: 2.114 ± 0.329
0.0AsnXaa: 0.0 ± 0.0
Pro
2.405ProAla: 2.405 ± 0.466
0.219ProCys: 0.219 ± 0.12
2.551ProAsp: 2.551 ± 0.504
2.624ProGlu: 2.624 ± 0.429
1.531ProPhe: 1.531 ± 0.38
2.187ProGly: 2.187 ± 0.363
1.02ProHis: 1.02 ± 0.332
2.478ProIle: 2.478 ± 0.44
2.405ProLys: 2.405 ± 0.601
2.478ProLeu: 2.478 ± 0.476
0.51ProMet: 0.51 ± 0.242
2.405ProAsn: 2.405 ± 0.53
1.093ProPro: 1.093 ± 0.274
0.656ProGln: 0.656 ± 0.263
1.239ProArg: 1.239 ± 0.234
2.551ProSer: 2.551 ± 0.485
1.677ProThr: 1.677 ± 0.385
2.26ProVal: 2.26 ± 0.446
0.51ProTrp: 0.51 ± 0.173
1.093ProTyr: 1.093 ± 0.303
0.0ProXaa: 0.0 ± 0.0
Gln
3.863GlnAla: 3.863 ± 0.604
0.219GlnCys: 0.219 ± 0.12
1.531GlnAsp: 1.531 ± 0.295
2.697GlnGlu: 2.697 ± 0.527
1.531GlnPhe: 1.531 ± 0.343
2.405GlnGly: 2.405 ± 0.384
0.51GlnHis: 0.51 ± 0.186
3.353GlnIle: 3.353 ± 0.579
3.645GlnLys: 3.645 ± 0.533
4.228GlnLeu: 4.228 ± 0.521
1.531GlnMet: 1.531 ± 0.316
1.968GlnAsn: 1.968 ± 0.376
1.385GlnPro: 1.385 ± 0.308
1.093GlnGln: 1.093 ± 0.333
2.333GlnArg: 2.333 ± 0.517
1.749GlnSer: 1.749 ± 0.326
2.26GlnThr: 2.26 ± 0.431
2.989GlnVal: 2.989 ± 0.398
0.292GlnTrp: 0.292 ± 0.122
0.729GlnTyr: 0.729 ± 0.275
0.0GlnXaa: 0.0 ± 0.0
Arg
2.26ArgAla: 2.26 ± 0.42
0.437ArgCys: 0.437 ± 0.162
2.405ArgAsp: 2.405 ± 0.346
4.155ArgGlu: 4.155 ± 0.707
2.114ArgPhe: 2.114 ± 0.375
2.624ArgGly: 2.624 ± 0.601
0.583ArgHis: 0.583 ± 0.167
4.009ArgIle: 4.009 ± 0.476
5.175ArgLys: 5.175 ± 0.737
4.228ArgLeu: 4.228 ± 0.526
1.02ArgMet: 1.02 ± 0.242
2.551ArgAsn: 2.551 ± 0.557
2.405ArgPro: 2.405 ± 0.356
1.822ArgGln: 1.822 ± 0.392
2.697ArgArg: 2.697 ± 0.454
2.478ArgSer: 2.478 ± 0.455
2.187ArgThr: 2.187 ± 0.382
2.916ArgVal: 2.916 ± 0.59
1.02ArgTrp: 1.02 ± 0.351
2.551ArgTyr: 2.551 ± 0.357
0.0ArgXaa: 0.0 ± 0.0
Ser
3.499SerAla: 3.499 ± 0.623
0.146SerCys: 0.146 ± 0.103
2.551SerAsp: 2.551 ± 0.509
4.811SerGlu: 4.811 ± 0.591
3.134SerPhe: 3.134 ± 0.482
3.645SerGly: 3.645 ± 0.608
0.802SerHis: 0.802 ± 0.293
3.717SerIle: 3.717 ± 0.461
5.321SerLys: 5.321 ± 0.656
5.102SerLeu: 5.102 ± 0.63
1.677SerMet: 1.677 ± 0.334
3.134SerAsn: 3.134 ± 0.773
2.405SerPro: 2.405 ± 0.389
1.749SerGln: 1.749 ± 0.486
2.916SerArg: 2.916 ± 0.446
4.592SerSer: 4.592 ± 0.84
3.936SerThr: 3.936 ± 0.554
3.936SerVal: 3.936 ± 0.544
0.802SerTrp: 0.802 ± 0.247
2.114SerTyr: 2.114 ± 0.478
0.0SerXaa: 0.0 ± 0.0
Thr
3.134ThrAla: 3.134 ± 0.548
0.583ThrCys: 0.583 ± 0.205
2.916ThrAsp: 2.916 ± 0.51
3.499ThrGlu: 3.499 ± 0.528
2.26ThrPhe: 2.26 ± 0.344
4.009ThrGly: 4.009 ± 0.519
0.948ThrHis: 0.948 ± 0.236
4.665ThrIle: 4.665 ± 0.673
3.79ThrLys: 3.79 ± 0.559
4.373ThrLeu: 4.373 ± 0.501
1.312ThrMet: 1.312 ± 0.344
2.26ThrAsn: 2.26 ± 0.438
2.333ThrPro: 2.333 ± 0.393
1.895ThrGln: 1.895 ± 0.301
2.041ThrArg: 2.041 ± 0.408
3.645ThrSer: 3.645 ± 0.527
2.989ThrThr: 2.989 ± 0.424
4.009ThrVal: 4.009 ± 0.511
0.583ThrTrp: 0.583 ± 0.192
2.187ThrTyr: 2.187 ± 0.443
0.0ThrXaa: 0.0 ± 0.0
Val
3.353ValAla: 3.353 ± 0.466
1.02ValCys: 1.02 ± 0.257
3.717ValAsp: 3.717 ± 0.435
4.519ValGlu: 4.519 ± 0.584
2.77ValPhe: 2.77 ± 0.538
2.624ValGly: 2.624 ± 0.46
1.093ValHis: 1.093 ± 0.28
5.613ValIle: 5.613 ± 0.923
5.613ValLys: 5.613 ± 0.608
4.738ValLeu: 4.738 ± 0.546
1.895ValMet: 1.895 ± 0.359
2.843ValAsn: 2.843 ± 0.461
2.187ValPro: 2.187 ± 0.359
1.677ValGln: 1.677 ± 0.384
2.989ValArg: 2.989 ± 0.448
5.248ValSer: 5.248 ± 0.557
3.572ValThr: 3.572 ± 0.548
4.155ValVal: 4.155 ± 0.66
1.312ValTrp: 1.312 ± 0.278
1.968ValTyr: 1.968 ± 0.325
0.0ValXaa: 0.0 ± 0.0
Trp
0.802TrpAla: 0.802 ± 0.255
0.219TrpCys: 0.219 ± 0.112
0.875TrpAsp: 0.875 ± 0.293
0.948TrpGlu: 0.948 ± 0.232
0.875TrpPhe: 0.875 ± 0.277
0.437TrpGly: 0.437 ± 0.158
0.583TrpHis: 0.583 ± 0.219
0.875TrpIle: 0.875 ± 0.311
1.385TrpLys: 1.385 ± 0.317
1.458TrpLeu: 1.458 ± 0.3
0.219TrpMet: 0.219 ± 0.107
0.656TrpAsn: 0.656 ± 0.245
0.219TrpPro: 0.219 ± 0.15
0.364TrpGln: 0.364 ± 0.156
0.802TrpArg: 0.802 ± 0.232
0.802TrpSer: 0.802 ± 0.218
0.146TrpThr: 0.146 ± 0.1
0.875TrpVal: 0.875 ± 0.267
0.437TrpTrp: 0.437 ± 0.229
0.656TrpTyr: 0.656 ± 0.221
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.041TyrAla: 2.041 ± 0.434
0.292TyrCys: 0.292 ± 0.14
2.551TyrAsp: 2.551 ± 0.499
3.353TyrGlu: 3.353 ± 0.403
1.312TyrPhe: 1.312 ± 0.288
1.968TyrGly: 1.968 ± 0.417
0.656TyrHis: 0.656 ± 0.249
2.551TyrIle: 2.551 ± 0.527
2.77TyrLys: 2.77 ± 0.466
3.061TyrLeu: 3.061 ± 0.556
0.729TyrMet: 0.729 ± 0.27
1.385TyrAsn: 1.385 ± 0.303
1.093TyrPro: 1.093 ± 0.313
1.604TyrGln: 1.604 ± 0.406
2.041TyrArg: 2.041 ± 0.363
1.458TyrSer: 1.458 ± 0.333
1.895TyrThr: 1.895 ± 0.417
2.989TyrVal: 2.989 ± 0.468
0.364TyrTrp: 0.364 ± 0.232
1.604TyrTyr: 1.604 ± 0.381
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (13720 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski