Prochlorococcus phage P-HS2
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M9Z6J6|A0A6M9Z6J6_9CAUD Uncharacterized protein OS=Prochlorococcus phage P-HS2 OX=2740745 GN=P-HS2_00057 PE=4 SV=1
MM1 pKa = 7.74 TYY3 pKa = 10.44 PSRR6 pKa = 11.84 FPSDD10 pKa = 3.54 PYY12 pKa = 10.58 EE13 pKa = 4.05 GQIFYY18 pKa = 10.93 DD19 pKa = 3.83 ADD21 pKa = 3.47 TDD23 pKa = 3.94 RR24 pKa = 11.84 TFEE27 pKa = 3.96 YY28 pKa = 10.23 QRR30 pKa = 11.84 RR31 pKa = 11.84 DD32 pKa = 3.11 ILEE35 pKa = 3.75 QMINCSKK42 pKa = 10.45 EE43 pKa = 3.5 VYY45 pKa = 9.6 YY46 pKa = 10.29 WFDD49 pKa = 2.86 ISKK52 pKa = 10.47 EE53 pKa = 3.87 II54 pKa = 4.82
Molecular weight: 6.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.015
IPC2_protein 4.368
IPC_protein 4.215
Toseland 4.024
ProMoST 4.355
Dawson 4.177
Bjellqvist 4.342
Wikipedia 4.101
Rodwell 4.037
Grimsley 3.948
Solomon 4.164
Lehninger 4.126
Nozaki 4.304
DTASelect 4.482
Thurlkill 4.075
EMBOSS 4.113
Sillero 4.329
Patrickios 1.977
IPC_peptide 4.164
IPC2_peptide 4.304
IPC2.peptide.svr19 4.225
Protein with the highest isoelectric point:
>tr|A0A6M9Z5R2|A0A6M9Z5R2_9CAUD Uncharacterized protein OS=Prochlorococcus phage P-HS2 OX=2740745 GN=P-HS2_00051 PE=4 SV=1
MM1 pKa = 8.05 PLRR4 pKa = 11.84 KK5 pKa = 9.81 GKK7 pKa = 7.72 SQKK10 pKa = 10.19 IISANIRR17 pKa = 11.84 KK18 pKa = 9.25 LMRR21 pKa = 11.84 EE22 pKa = 3.95 GKK24 pKa = 7.53 TLKK27 pKa = 9.89 QAQAIALTTAKK38 pKa = 9.91 KK39 pKa = 10.09 RR40 pKa = 11.84 KK41 pKa = 9.37 RR42 pKa = 11.84 KK43 pKa = 9.54
Molecular weight: 4.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.245
IPC2_protein 10.57
IPC_protein 12.018
Toseland 12.237
ProMoST 12.676
Dawson 12.237
Bjellqvist 12.193
Wikipedia 12.676
Rodwell 12.31
Grimsley 12.281
Solomon 12.705
Lehninger 12.603
Nozaki 12.223
DTASelect 12.193
Thurlkill 12.223
EMBOSS 12.72
Sillero 12.223
Patrickios 12.047
IPC_peptide 12.705
IPC2_peptide 11.667
IPC2.peptide.svr19 8.961
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
64
0
64
11111
32
776
173.6
19.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.469 ± 0.466
0.99 ± 0.151
5.796 ± 0.322
6.219 ± 0.371
4.077 ± 0.197
6.633 ± 0.572
1.359 ± 0.165
6.534 ± 0.241
7.596 ± 0.559
7.902 ± 0.303
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.142 ± 0.179
5.4 ± 0.227
3.762 ± 0.346
4.266 ± 0.232
4.419 ± 0.334
6.795 ± 0.334
7.47 ± 0.373
6.273 ± 0.259
1.008 ± 0.121
2.889 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here