Amino acid dipepetide frequency for Prochlorococcus phage P-HS2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.721AlaAla: 9.721 ± 1.159
0.54AlaCys: 0.54 ± 0.233
4.32AlaAsp: 4.32 ± 0.708
4.41AlaGlu: 4.41 ± 0.649
3.06AlaPhe: 3.06 ± 0.572
6.391AlaGly: 6.391 ± 0.914
0.9AlaHis: 0.9 ± 0.37
6.121AlaIle: 6.121 ± 0.559
6.571AlaLys: 6.571 ± 0.917
6.301AlaLeu: 6.301 ± 0.719
1.71AlaMet: 1.71 ± 0.364
5.131AlaAsn: 5.131 ± 0.843
2.97AlaPro: 2.97 ± 0.412
2.97AlaGln: 2.97 ± 0.501
3.78AlaArg: 3.78 ± 0.661
5.671AlaSer: 5.671 ± 0.764
6.121AlaThr: 6.121 ± 0.842
5.311AlaVal: 5.311 ± 0.828
0.9AlaTrp: 0.9 ± 0.252
1.8AlaTyr: 1.8 ± 0.35
0.0AlaXaa: 0.0 ± 0.0
Cys
0.45CysAla: 0.45 ± 0.243
0.0CysCys: 0.0 ± 0.0
0.72CysAsp: 0.72 ± 0.215
0.36CysGlu: 0.36 ± 0.164
0.45CysPhe: 0.45 ± 0.251
0.54CysGly: 0.54 ± 0.2
0.36CysHis: 0.36 ± 0.169
0.9CysIle: 0.9 ± 0.241
0.99CysLys: 0.99 ± 0.312
0.81CysLeu: 0.81 ± 0.221
0.09CysMet: 0.09 ± 0.095
0.72CysAsn: 0.72 ± 0.199
0.27CysPro: 0.27 ± 0.163
0.27CysGln: 0.27 ± 0.147
0.81CysArg: 0.81 ± 0.297
0.54CysSer: 0.54 ± 0.283
0.72CysThr: 0.72 ± 0.247
0.45CysVal: 0.45 ± 0.196
0.09CysTrp: 0.09 ± 0.093
0.36CysTyr: 0.36 ± 0.229
0.0CysXaa: 0.0 ± 0.0
Asp
4.32AspAla: 4.32 ± 0.636
0.81AspCys: 0.81 ± 0.269
3.33AspAsp: 3.33 ± 0.703
2.88AspGlu: 2.88 ± 0.525
3.51AspPhe: 3.51 ± 0.342
4.5AspGly: 4.5 ± 0.927
0.63AspHis: 0.63 ± 0.231
4.86AspIle: 4.86 ± 0.749
3.42AspLys: 3.42 ± 0.656
5.491AspLeu: 5.491 ± 0.78
0.99AspMet: 0.99 ± 0.304
3.51AspAsn: 3.51 ± 0.573
2.25AspPro: 2.25 ± 0.401
1.8AspGln: 1.8 ± 0.407
2.97AspArg: 2.97 ± 0.522
2.97AspSer: 2.97 ± 0.606
3.78AspThr: 3.78 ± 0.628
3.6AspVal: 3.6 ± 0.568
0.45AspTrp: 0.45 ± 0.216
1.89AspTyr: 1.89 ± 0.45
0.0AspXaa: 0.0 ± 0.0
Glu
6.301GluAla: 6.301 ± 0.752
0.54GluCys: 0.54 ± 0.247
3.42GluAsp: 3.42 ± 0.629
3.78GluGlu: 3.78 ± 0.751
2.61GluPhe: 2.61 ± 0.496
3.87GluGly: 3.87 ± 0.725
0.36GluHis: 0.36 ± 0.184
4.05GluIle: 4.05 ± 0.666
4.68GluLys: 4.68 ± 0.669
6.301GluLeu: 6.301 ± 0.62
1.89GluMet: 1.89 ± 0.341
2.7GluAsn: 2.7 ± 0.453
2.7GluPro: 2.7 ± 0.507
3.15GluGln: 3.15 ± 0.506
1.71GluArg: 1.71 ± 0.381
3.6GluSer: 3.6 ± 0.62
3.78GluThr: 3.78 ± 0.599
3.78GluVal: 3.78 ± 0.536
1.08GluTrp: 1.08 ± 0.308
1.89GluTyr: 1.89 ± 0.398
0.0GluXaa: 0.0 ± 0.0
Phe
2.07PheAla: 2.07 ± 0.36
0.36PheCys: 0.36 ± 0.216
3.15PheAsp: 3.15 ± 0.477
2.88PheGlu: 2.88 ± 0.624
1.8PhePhe: 1.8 ± 0.418
3.15PheGly: 3.15 ± 0.613
0.81PheHis: 0.81 ± 0.258
2.88PheIle: 2.88 ± 0.424
2.43PheLys: 2.43 ± 0.461
2.25PheLeu: 2.25 ± 0.341
0.9PheMet: 0.9 ± 0.311
2.07PheAsn: 2.07 ± 0.403
1.62PhePro: 1.62 ± 0.356
1.44PheGln: 1.44 ± 0.362
2.52PheArg: 2.52 ± 0.427
3.15PheSer: 3.15 ± 0.574
3.42PheThr: 3.42 ± 0.54
2.52PheVal: 2.52 ± 0.419
0.54PheTrp: 0.54 ± 0.202
0.81PheTyr: 0.81 ± 0.298
0.0PheXaa: 0.0 ± 0.0
Gly
5.761GlyAla: 5.761 ± 0.765
0.45GlyCys: 0.45 ± 0.218
3.69GlyAsp: 3.69 ± 0.891
3.33GlyGlu: 3.33 ± 0.481
3.15GlyPhe: 3.15 ± 0.697
5.311GlyGly: 5.311 ± 1.014
0.9GlyHis: 0.9 ± 0.277
4.59GlyIle: 4.59 ± 0.752
4.23GlyLys: 4.23 ± 0.753
5.221GlyLeu: 5.221 ± 0.649
1.44GlyMet: 1.44 ± 0.483
3.51GlyAsn: 3.51 ± 0.562
0.81GlyPro: 0.81 ± 0.215
2.97GlyGln: 2.97 ± 0.497
1.98GlyArg: 1.98 ± 0.337
5.671GlySer: 5.671 ± 0.797
6.121GlyThr: 6.121 ± 0.93
4.41GlyVal: 4.41 ± 0.658
0.81GlyTrp: 0.81 ± 0.244
1.98GlyTyr: 1.98 ± 0.481
0.0GlyXaa: 0.0 ± 0.0
His
0.45HisAla: 0.45 ± 0.184
0.09HisCys: 0.09 ± 0.085
0.81HisAsp: 0.81 ± 0.279
0.72HisGlu: 0.72 ± 0.229
0.45HisPhe: 0.45 ± 0.215
1.08HisGly: 1.08 ± 0.3
0.09HisHis: 0.09 ± 0.084
0.54HisIle: 0.54 ± 0.214
0.9HisLys: 0.9 ± 0.245
1.44HisLeu: 1.44 ± 0.323
0.36HisMet: 0.36 ± 0.202
0.99HisAsn: 0.99 ± 0.334
0.45HisPro: 0.45 ± 0.174
1.17HisGln: 1.17 ± 0.388
0.81HisArg: 0.81 ± 0.271
0.81HisSer: 0.81 ± 0.233
0.9HisThr: 0.9 ± 0.281
0.72HisVal: 0.72 ± 0.252
0.09HisTrp: 0.09 ± 0.094
0.72HisTyr: 0.72 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
5.401IleAla: 5.401 ± 0.579
0.72IleCys: 0.72 ± 0.21
4.68IleAsp: 4.68 ± 0.513
4.77IleGlu: 4.77 ± 0.678
1.35IlePhe: 1.35 ± 0.302
3.96IleGly: 3.96 ± 1.058
1.26IleHis: 1.26 ± 0.349
2.7IleIle: 2.7 ± 0.477
5.851IleLys: 5.851 ± 0.702
4.23IleLeu: 4.23 ± 0.792
1.17IleMet: 1.17 ± 0.312
3.24IleAsn: 3.24 ± 0.498
3.33IlePro: 3.33 ± 0.577
2.52IleGln: 2.52 ± 0.477
2.88IleArg: 2.88 ± 0.567
4.32IleSer: 4.32 ± 0.723
6.571IleThr: 6.571 ± 0.974
3.96IleVal: 3.96 ± 0.597
0.36IleTrp: 0.36 ± 0.148
1.98IleTyr: 1.98 ± 0.359
0.0IleXaa: 0.0 ± 0.0
Lys
6.121LysAla: 6.121 ± 0.798
0.36LysCys: 0.36 ± 0.226
4.05LysAsp: 4.05 ± 0.632
4.68LysGlu: 4.68 ± 0.865
2.7LysPhe: 2.7 ± 0.606
4.32LysGly: 4.32 ± 0.7
0.81LysHis: 0.81 ± 0.289
5.581LysIle: 5.581 ± 0.519
9.091LysLys: 9.091 ± 1.139
5.941LysLeu: 5.941 ± 0.696
2.16LysMet: 2.16 ± 0.467
3.78LysAsn: 3.78 ± 0.542
2.43LysPro: 2.43 ± 0.509
3.24LysGln: 3.24 ± 0.568
4.5LysArg: 4.5 ± 0.724
3.15LysSer: 3.15 ± 0.555
4.77LysThr: 4.77 ± 0.632
5.041LysVal: 5.041 ± 0.697
0.45LysTrp: 0.45 ± 0.201
2.79LysTyr: 2.79 ± 0.493
0.0LysXaa: 0.0 ± 0.0
Leu
7.291LeuAla: 7.291 ± 0.779
0.99LeuCys: 0.99 ± 0.373
4.77LeuAsp: 4.77 ± 0.802
5.761LeuGlu: 5.761 ± 0.709
2.97LeuPhe: 2.97 ± 0.465
5.311LeuGly: 5.311 ± 0.724
1.08LeuHis: 1.08 ± 0.402
5.041LeuIle: 5.041 ± 0.595
5.581LeuLys: 5.581 ± 0.751
4.41LeuLeu: 4.41 ± 0.576
1.62LeuMet: 1.62 ± 0.362
5.041LeuAsn: 5.041 ± 0.593
3.96LeuPro: 3.96 ± 0.567
3.69LeuGln: 3.69 ± 0.466
2.79LeuArg: 2.79 ± 0.415
5.941LeuSer: 5.941 ± 0.779
5.941LeuThr: 5.941 ± 0.679
4.23LeuVal: 4.23 ± 0.599
0.63LeuTrp: 0.63 ± 0.227
1.98LeuTyr: 1.98 ± 0.428
0.0LeuXaa: 0.0 ± 0.0
Met
2.7MetAla: 2.7 ± 0.591
0.27MetCys: 0.27 ± 0.148
1.17MetAsp: 1.17 ± 0.282
1.53MetGlu: 1.53 ± 0.344
0.54MetPhe: 0.54 ± 0.229
0.9MetGly: 0.9 ± 0.212
0.36MetHis: 0.36 ± 0.21
1.26MetIle: 1.26 ± 0.28
2.43MetLys: 2.43 ± 0.573
1.53MetLeu: 1.53 ± 0.395
0.36MetMet: 0.36 ± 0.167
0.63MetAsn: 0.63 ± 0.226
0.54MetPro: 0.54 ± 0.183
0.9MetGln: 0.9 ± 0.291
0.99MetArg: 0.99 ± 0.316
1.8MetSer: 1.8 ± 0.327
1.71MetThr: 1.71 ± 0.43
0.54MetVal: 0.54 ± 0.215
0.63MetTrp: 0.63 ± 0.202
0.63MetTyr: 0.63 ± 0.187
0.0MetXaa: 0.0 ± 0.0
Asn
4.5AsnAla: 4.5 ± 0.482
1.62AsnCys: 1.62 ± 0.342
2.79AsnAsp: 2.79 ± 0.353
2.52AsnGlu: 2.52 ± 0.527
1.71AsnPhe: 1.71 ± 0.324
3.78AsnGly: 3.78 ± 0.547
0.45AsnHis: 0.45 ± 0.197
4.14AsnIle: 4.14 ± 0.608
3.06AsnLys: 3.06 ± 0.466
5.311AsnLeu: 5.311 ± 0.659
0.99AsnMet: 0.99 ± 0.274
3.24AsnAsn: 3.24 ± 0.562
2.07AsnPro: 2.07 ± 0.506
2.79AsnGln: 2.79 ± 0.472
2.61AsnArg: 2.61 ± 0.532
3.78AsnSer: 3.78 ± 0.713
3.15AsnThr: 3.15 ± 0.533
3.78AsnVal: 3.78 ± 0.565
0.36AsnTrp: 0.36 ± 0.192
1.35AsnTyr: 1.35 ± 0.35
0.0AsnXaa: 0.0 ± 0.0
Pro
2.34ProAla: 2.34 ± 0.397
0.27ProCys: 0.27 ± 0.187
3.24ProAsp: 3.24 ± 0.648
3.78ProGlu: 3.78 ± 0.878
1.53ProPhe: 1.53 ± 0.382
0.0ProGly: 0.0 ± 0.0
0.63ProHis: 0.63 ± 0.22
1.89ProIle: 1.89 ± 0.295
3.42ProLys: 3.42 ± 0.648
1.71ProLeu: 1.71 ± 0.381
0.99ProMet: 0.99 ± 0.3
2.16ProAsn: 2.16 ± 0.411
1.89ProPro: 1.89 ± 0.396
1.62ProGln: 1.62 ± 0.411
1.53ProArg: 1.53 ± 0.403
3.24ProSer: 3.24 ± 0.629
3.42ProThr: 3.42 ± 0.661
2.7ProVal: 2.7 ± 0.499
0.0ProTrp: 0.0 ± 0.0
1.26ProTyr: 1.26 ± 0.325
0.0ProXaa: 0.0 ± 0.0
Gln
4.14GlnAla: 4.14 ± 0.599
0.45GlnCys: 0.45 ± 0.191
1.62GlnAsp: 1.62 ± 0.309
1.98GlnGlu: 1.98 ± 0.456
2.34GlnPhe: 2.34 ± 0.431
2.88GlnGly: 2.88 ± 0.399
0.72GlnHis: 0.72 ± 0.234
3.51GlnIle: 3.51 ± 0.556
3.42GlnLys: 3.42 ± 0.562
3.78GlnLeu: 3.78 ± 0.708
1.08GlnMet: 1.08 ± 0.35
2.61GlnAsn: 2.61 ± 0.444
1.35GlnPro: 1.35 ± 0.356
1.62GlnGln: 1.62 ± 0.398
2.07GlnArg: 2.07 ± 0.462
2.7GlnSer: 2.7 ± 0.436
2.7GlnThr: 2.7 ± 0.42
2.16GlnVal: 2.16 ± 0.423
0.09GlnTrp: 0.09 ± 0.075
1.44GlnTyr: 1.44 ± 0.415
0.0GlnXaa: 0.0 ± 0.0
Arg
2.7ArgAla: 2.7 ± 0.595
0.36ArgCys: 0.36 ± 0.176
2.34ArgAsp: 2.34 ± 0.44
3.33ArgGlu: 3.33 ± 0.63
1.98ArgPhe: 1.98 ± 0.457
1.89ArgGly: 1.89 ± 0.372
0.45ArgHis: 0.45 ± 0.235
3.06ArgIle: 3.06 ± 0.532
4.23ArgLys: 4.23 ± 0.732
3.69ArgLeu: 3.69 ± 0.631
1.26ArgMet: 1.26 ± 0.38
2.7ArgAsn: 2.7 ± 0.479
1.44ArgPro: 1.44 ± 0.305
2.7ArgGln: 2.7 ± 0.528
1.98ArgArg: 1.98 ± 0.454
2.79ArgSer: 2.79 ± 0.555
2.25ArgThr: 2.25 ± 0.436
3.06ArgVal: 3.06 ± 0.455
0.72ArgTrp: 0.72 ± 0.25
1.26ArgTyr: 1.26 ± 0.449
0.0ArgXaa: 0.0 ± 0.0
Ser
7.201SerAla: 7.201 ± 0.903
0.63SerCys: 0.63 ± 0.232
3.15SerAsp: 3.15 ± 0.427
3.24SerGlu: 3.24 ± 0.586
3.33SerPhe: 3.33 ± 0.44
7.561SerGly: 7.561 ± 0.875
0.9SerHis: 0.9 ± 0.228
3.33SerIle: 3.33 ± 0.473
4.5SerLys: 4.5 ± 0.558
5.851SerLeu: 5.851 ± 0.796
1.53SerMet: 1.53 ± 0.334
3.51SerAsn: 3.51 ± 0.47
1.89SerPro: 1.89 ± 0.473
2.52SerGln: 2.52 ± 0.531
2.97SerArg: 2.97 ± 0.484
5.131SerSer: 5.131 ± 0.876
4.23SerThr: 4.23 ± 0.662
3.6SerVal: 3.6 ± 0.54
1.17SerTrp: 1.17 ± 0.368
1.71SerTyr: 1.71 ± 0.385
0.0SerXaa: 0.0 ± 0.0
Thr
5.671ThrAla: 5.671 ± 0.599
0.18ThrCys: 0.18 ± 0.108
4.05ThrAsp: 4.05 ± 0.534
4.68ThrGlu: 4.68 ± 0.75
3.33ThrPhe: 3.33 ± 0.547
5.581ThrGly: 5.581 ± 0.734
0.81ThrHis: 0.81 ± 0.336
4.86ThrIle: 4.86 ± 0.683
4.59ThrLys: 4.59 ± 0.658
6.661ThrLeu: 6.661 ± 0.838
1.35ThrMet: 1.35 ± 0.374
3.24ThrAsn: 3.24 ± 0.533
3.78ThrPro: 3.78 ± 0.589
3.06ThrGln: 3.06 ± 0.511
2.52ThrArg: 2.52 ± 0.482
5.221ThrSer: 5.221 ± 0.726
7.111ThrThr: 7.111 ± 1.106
5.851ThrVal: 5.851 ± 0.856
0.45ThrTrp: 0.45 ± 0.229
1.71ThrTyr: 1.71 ± 0.425
0.0ThrXaa: 0.0 ± 0.0
Val
4.59ValAla: 4.59 ± 0.846
0.99ValCys: 0.99 ± 0.316
3.78ValAsp: 3.78 ± 0.634
4.14ValGlu: 4.14 ± 0.619
2.7ValPhe: 2.7 ± 0.527
3.06ValGly: 3.06 ± 0.459
1.08ValHis: 1.08 ± 0.37
4.23ValIle: 4.23 ± 0.71
3.96ValLys: 3.96 ± 0.613
4.95ValLeu: 4.95 ± 0.749
1.08ValMet: 1.08 ± 0.305
3.06ValAsn: 3.06 ± 0.571
2.07ValPro: 2.07 ± 0.47
2.88ValGln: 2.88 ± 0.396
2.34ValArg: 2.34 ± 0.396
4.68ValSer: 4.68 ± 0.676
5.581ValThr: 5.581 ± 1.006
4.86ValVal: 4.86 ± 0.608
0.72ValTrp: 0.72 ± 0.212
1.98ValTyr: 1.98 ± 0.385
0.0ValXaa: 0.0 ± 0.0
Trp
0.81TrpAla: 0.81 ± 0.281
0.0TrpCys: 0.0 ± 0.0
0.54TrpAsp: 0.54 ± 0.228
0.63TrpGlu: 0.63 ± 0.245
0.63TrpPhe: 0.63 ± 0.23
0.63TrpGly: 0.63 ± 0.26
0.36TrpHis: 0.36 ± 0.167
0.27TrpIle: 0.27 ± 0.128
0.81TrpLys: 0.81 ± 0.257
0.9TrpLeu: 0.9 ± 0.315
0.18TrpMet: 0.18 ± 0.107
0.45TrpAsn: 0.45 ± 0.203
0.27TrpPro: 0.27 ± 0.153
0.27TrpGln: 0.27 ± 0.147
0.72TrpArg: 0.72 ± 0.255
1.35TrpSer: 1.35 ± 0.282
0.27TrpThr: 0.27 ± 0.154
0.72TrpVal: 0.72 ± 0.253
0.18TrpTrp: 0.18 ± 0.113
0.09TrpTyr: 0.09 ± 0.107
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.16TyrAla: 2.16 ± 0.452
0.27TyrCys: 0.27 ± 0.16
2.34TyrAsp: 2.34 ± 0.496
2.79TyrGlu: 2.79 ± 0.551
0.63TyrPhe: 0.63 ± 0.221
1.62TyrGly: 1.62 ± 0.339
0.63TyrHis: 0.63 ± 0.244
1.53TyrIle: 1.53 ± 0.298
1.8TyrLys: 1.8 ± 0.406
2.25TyrLeu: 2.25 ± 0.548
0.18TyrMet: 0.18 ± 0.14
1.71TyrAsn: 1.71 ± 0.342
1.53TyrPro: 1.53 ± 0.4
1.08TyrGln: 1.08 ± 0.356
1.71TyrArg: 1.71 ± 0.305
1.44TyrSer: 1.44 ± 0.361
2.16TyrThr: 2.16 ± 0.391
1.44TyrVal: 1.44 ± 0.286
0.36TyrTrp: 0.36 ± 0.163
1.26TyrTyr: 1.26 ± 0.324
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (11111 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski