Candidatus Filomicrobium marinum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Filomicrobium

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4045 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D6JDZ7|A0A0D6JDZ7_9RHIZ Sulfurtransferase OS=Candidatus Filomicrobium marinum OX=1608628 GN=YBN1229_v1_1293 PE=4 SV=1
MM1 pKa = 7.24TALKK5 pKa = 10.48LVLGTVAASAFWLSAAQATTIINRR29 pKa = 11.84DD30 pKa = 3.34PSAHH34 pKa = 6.24NLRR37 pKa = 11.84IVEE40 pKa = 4.33GDD42 pKa = 3.27QEE44 pKa = 4.31RR45 pKa = 11.84VVTAEE50 pKa = 4.19PSQQLDD56 pKa = 3.86EE57 pKa = 5.03LCSSSCSLFFDD68 pKa = 5.38DD69 pKa = 6.52DD70 pKa = 3.86PDD72 pKa = 4.56AYY74 pKa = 10.13EE75 pKa = 4.2IAAGDD80 pKa = 3.57IVSIEE85 pKa = 4.18DD86 pKa = 3.44GEE88 pKa = 4.56LYY90 pKa = 10.65YY91 pKa = 10.4EE92 pKa = 4.63EE93 pKa = 5.54PSALPEE99 pKa = 4.38APSMPADD106 pKa = 3.65ANNYY110 pKa = 9.1GPANQQ115 pKa = 3.89

Molecular weight: 12.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D6JIS5|A0A0D6JIS5_9RHIZ Putative enzyme OS=Candidatus Filomicrobium marinum OX=1608628 GN=YBN1229_v1_3301 PE=3 SV=1
MM1 pKa = 6.99EE2 pKa = 4.4QVFPSLFALLPVRR15 pKa = 11.84WLRR18 pKa = 11.84VATGAARR25 pKa = 11.84IRR27 pKa = 11.84VILSLKK33 pKa = 9.88PNGARR38 pKa = 11.84ARR40 pKa = 11.84LWRR43 pKa = 11.84KK44 pKa = 9.05LRR46 pKa = 11.84SSSPMAALRR55 pKa = 11.84SSRR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84EE62 pKa = 3.62PRR64 pKa = 3.13

Molecular weight: 7.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4045

0

4045

1183516

12

2959

292.6

31.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.6 ± 0.051

0.933 ± 0.013

5.568 ± 0.028

5.913 ± 0.04

3.719 ± 0.025

8.175 ± 0.065

2.091 ± 0.02

5.462 ± 0.026

3.724 ± 0.035

9.873 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.379 ± 0.02

2.862 ± 0.029

5.083 ± 0.031

3.217 ± 0.022

6.989 ± 0.039

6.003 ± 0.032

5.426 ± 0.029

7.37 ± 0.03

1.343 ± 0.014

2.27 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski