Pseudorhizobium pelagicum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Pseudorhizobium

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4417 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A081MES3|A0A081MES3_9RHIZ UPF0060 membrane protein GV67_07810 OS=Pseudorhizobium pelagicum OX=1509405 GN=GV67_07810 PE=3 SV=1
MM1 pKa = 7.56AVLDD5 pKa = 4.33QITEE9 pKa = 4.41EE10 pKa = 3.84ISHH13 pKa = 5.92FVGFFHH19 pKa = 7.65LSLEE23 pKa = 4.28EE24 pKa = 3.72ARR26 pKa = 11.84QRR28 pKa = 11.84EE29 pKa = 4.56SYY31 pKa = 11.42NDD33 pKa = 3.45FSFKK37 pKa = 8.4TTLHH41 pKa = 6.14GVDD44 pKa = 3.23SRR46 pKa = 11.84LVEE49 pKa = 4.28EE50 pKa = 5.07APFAAPHH57 pKa = 6.53ALDD60 pKa = 5.45DD61 pKa = 4.1YY62 pKa = 11.9DD63 pKa = 5.69PILNYY68 pKa = 10.46RR69 pKa = 11.84PVAPIFPAAPAFYY82 pKa = 10.35LPPALLDD89 pKa = 3.5VAPEE93 pKa = 3.89NLRR96 pKa = 11.84VEE98 pKa = 4.73VFAQEE103 pKa = 3.86ASLRR107 pKa = 11.84TPMPFHH113 pKa = 6.72TGGKK117 pKa = 7.48ATAPALEE124 pKa = 4.72PPGSLATYY132 pKa = 8.18AQQHH136 pKa = 5.75IVLSDD141 pKa = 3.35NDD143 pKa = 3.68VFSVGGHH150 pKa = 5.86GFSISGNPVALAEE163 pKa = 4.41LLSMAEE169 pKa = 4.06PVLAVSPLGEE179 pKa = 4.17LEE181 pKa = 4.5APGSAAEE188 pKa = 4.42MIDD191 pKa = 3.68LVEE194 pKa = 4.46TVAEE198 pKa = 4.25SLEE201 pKa = 3.93HH202 pKa = 7.02LIDD205 pKa = 4.02GAGSHH210 pKa = 5.73QVAQASTLSGVYY222 pKa = 9.59VNGVAVEE229 pKa = 3.99QAPSLEE235 pKa = 5.0DD236 pKa = 3.13YY237 pKa = 11.09HH238 pKa = 9.11SFDD241 pKa = 4.16EE242 pKa = 5.43GEE244 pKa = 4.29DD245 pKa = 3.3DD246 pKa = 4.11GAIVEE251 pKa = 4.64AEE253 pKa = 3.76WGGEE257 pKa = 4.14GSQPPPASVTITMGDD272 pKa = 3.18NTLVNDD278 pKa = 4.03VLVKK282 pKa = 10.73NLWTAAKK289 pKa = 8.48VTVVQGDD296 pKa = 4.04HH297 pKa = 6.68VEE299 pKa = 4.31VNAVVQINAVWDD311 pKa = 3.71TDD313 pKa = 4.51SIGSTVAGWGASNSANDD330 pKa = 4.17LFNIASFDD338 pKa = 3.84HH339 pKa = 7.49DD340 pKa = 4.78DD341 pKa = 3.93SAGSQQLAGASRR353 pKa = 11.84PDD355 pKa = 4.3LYY357 pKa = 10.51PSYY360 pKa = 10.74WNVTTVTGDD369 pKa = 3.35LMIVNWIEE377 pKa = 3.59QLIFMSDD384 pKa = 2.98ADD386 pKa = 3.93MGIVSASGAYY396 pKa = 8.37STIISGDD403 pKa = 3.27NTAVNHH409 pKa = 4.6TTITEE414 pKa = 4.14LGFGYY419 pKa = 10.59DD420 pKa = 4.11LIIIGGSVYY429 pKa = 10.48DD430 pKa = 4.16ANIIQQVNVLFDD442 pKa = 3.74NDD444 pKa = 3.97AVTTTDD450 pKa = 3.05GFEE453 pKa = 4.03SAEE456 pKa = 4.17AGTVSASGNLLWNQASIYY474 pKa = 9.68TIGAAEE480 pKa = 4.13RR481 pKa = 11.84FGEE484 pKa = 4.46LSDD487 pKa = 3.97AYY489 pKa = 10.28RR490 pKa = 11.84DD491 pKa = 3.75AVASLQSGSANLSDD505 pKa = 4.57EE506 pKa = 4.3VLHH509 pKa = 7.12DD510 pKa = 3.75SAFAGLAGLRR520 pKa = 11.84VLHH523 pKa = 5.72VQGDD527 pKa = 4.74FINVQYY533 pKa = 10.74IKK535 pKa = 9.15QTSIIGDD542 pKa = 3.4NDD544 pKa = 3.8QILLAMDD551 pKa = 5.22ALTPNNDD558 pKa = 3.52ASWTVQAGGNTLINNAAILDD578 pKa = 4.0LDD580 pKa = 4.34SFGKK584 pKa = 9.18TYY586 pKa = 11.01VGGEE590 pKa = 4.09QYY592 pKa = 10.88SQEE595 pKa = 4.17TLIQANFISSQPEE608 pKa = 4.12LASHH612 pKa = 7.68DD613 pKa = 4.3PGTLATEE620 pKa = 4.18AVLFLDD626 pKa = 4.21DD627 pKa = 4.34TMLEE631 pKa = 4.16ADD633 pKa = 4.37PAPEE637 pKa = 4.81GGSYY641 pKa = 9.21WPADD645 pKa = 3.59SAGGQDD651 pKa = 5.78DD652 pKa = 4.28GLQTLLAA659 pKa = 4.9

Molecular weight: 69.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A081M8A6|A0A081M8A6_9RHIZ Chemotaxis protein CheY OS=Pseudorhizobium pelagicum OX=1509405 GN=GV67_19610 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.06LLSPMQSLPVRR13 pKa = 11.84AQQISPPLSRR23 pKa = 11.84PPSPGTRR30 pKa = 11.84PQPPLSQPLLPPSRR44 pKa = 11.84RR45 pKa = 11.84PLPLRR50 pKa = 11.84WPQPPRR56 pKa = 11.84PPPRR60 pKa = 11.84QPQSRR65 pKa = 11.84LRR67 pKa = 11.84KK68 pKa = 9.03PFPLQPPQPLLKK80 pKa = 10.01LQRR83 pKa = 11.84PFPPQLQPLIPMRR96 pKa = 11.84LPRR99 pKa = 11.84PRR101 pKa = 11.84PP102 pKa = 3.36

Molecular weight: 11.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4417

0

4417

1347192

37

2827

305.0

33.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.904 ± 0.049

0.79 ± 0.011

5.763 ± 0.031

5.962 ± 0.033

3.848 ± 0.025

8.393 ± 0.038

2.016 ± 0.02

5.53 ± 0.024

3.302 ± 0.03

10.094 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.694 ± 0.018

2.64 ± 0.018

4.95 ± 0.025

3.241 ± 0.021

6.776 ± 0.043

5.705 ± 0.03

5.374 ± 0.023

7.462 ± 0.025

1.266 ± 0.015

2.281 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski