Amino acid dipepetide frequency for Pseudorhizobium pelagicum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.031AlaAla: 16.031 ± 0.175	0.906AlaCys: 0.906 ± 0.027	6.784AlaAsp: 6.784 ± 0.079	7.643AlaGlu: 7.643 ± 0.078	4.563AlaPhe: 4.563 ± 0.069	10.286AlaGly: 10.286 ± 0.094	2.045AlaHis: 2.045 ± 0.045	6.56AlaIle: 6.56 ± 0.076	3.725AlaLys: 3.725 ± 0.063	12.703AlaLeu: 12.703 ± 0.123	3.657AlaMet: 3.657 ± 0.061	2.724AlaAsn: 2.724 ± 0.047	4.82AlaPro: 4.82 ± 0.083	3.746AlaGln: 3.746 ± 0.055	7.665AlaArg: 7.665 ± 0.086	6.589AlaSer: 6.589 ± 0.084	5.826AlaThr: 5.826 ± 0.078	8.879AlaVal: 8.879 ± 0.095	1.361AlaTrp: 1.361 ± 0.028	2.519AlaTyr: 2.519 ± 0.045	0.008AlaXaa: 0.008 ± 0.004
Cys	0.868CysAla: 0.868 ± 0.029	0.101CysCys: 0.101 ± 0.009	0.467CysAsp: 0.467 ± 0.019	0.424CysGlu: 0.424 ± 0.02	0.343CysPhe: 0.343 ± 0.016	0.89CysGly: 0.89 ± 0.032	0.225CysHis: 0.225 ± 0.014	0.394CysIle: 0.394 ± 0.02	0.171CysLys: 0.171 ± 0.012	0.825CysLeu: 0.825 ± 0.025	0.157CysMet: 0.157 ± 0.01	0.206CysAsn: 0.206 ± 0.012	0.345CysPro: 0.345 ± 0.015	0.221CysGln: 0.221 ± 0.014	0.586CysArg: 0.586 ± 0.019	0.462CysSer: 0.462 ± 0.018	0.373CysThr: 0.373 ± 0.018	0.554CysVal: 0.554 ± 0.019	0.096CysTrp: 0.096 ± 0.008	0.193CysTyr: 0.193 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	6.499AspAla: 6.499 ± 0.085	0.485AspCys: 0.485 ± 0.018	3.26AspAsp: 3.26 ± 0.06	3.737AspGlu: 3.737 ± 0.06	2.285AspPhe: 2.285 ± 0.041	5.188AspGly: 5.188 ± 0.082	1.245AspHis: 1.245 ± 0.034	3.352AspIle: 3.352 ± 0.05	1.664AspLys: 1.664 ± 0.036	6.159AspLeu: 6.159 ± 0.068	1.476AspMet: 1.476 ± 0.028	1.418AspAsn: 1.418 ± 0.037	3.38AspPro: 3.38 ± 0.052	1.781AspGln: 1.781 ± 0.044	4.295AspArg: 4.295 ± 0.061	2.147AspSer: 2.147 ± 0.04	2.582AspThr: 2.582 ± 0.045	4.181AspVal: 4.181 ± 0.058	0.925AspTrp: 0.925 ± 0.028	1.562AspTyr: 1.562 ± 0.039	0.006AspXaa: 0.006 ± 0.002
Glu	7.646GluAla: 7.646 ± 0.083	0.336GluCys: 0.336 ± 0.014	3.156GluAsp: 3.156 ± 0.053	3.72GluGlu: 3.72 ± 0.058	1.887GluPhe: 1.887 ± 0.036	4.572GluGly: 4.572 ± 0.063	1.205GluHis: 1.205 ± 0.03	3.839GluIle: 3.839 ± 0.053	2.487GluLys: 2.487 ± 0.049	5.335GluLeu: 5.335 ± 0.072	1.723GluMet: 1.723 ± 0.035	1.731GluAsn: 1.731 ± 0.037	2.798GluPro: 2.798 ± 0.057	2.183GluGln: 2.183 ± 0.048	4.872GluArg: 4.872 ± 0.072	2.373GluSer: 2.373 ± 0.048	3.842GluThr: 3.842 ± 0.051	4.143GluVal: 4.143 ± 0.056	0.696GluTrp: 0.696 ± 0.023	1.068GluTyr: 1.068 ± 0.031	0.007GluXaa: 0.007 ± 0.003
Phe	4.503PheAla: 4.503 ± 0.058	0.378PheCys: 0.378 ± 0.016	2.711PheAsp: 2.711 ± 0.045	2.184PheGlu: 2.184 ± 0.036	1.554PhePhe: 1.554 ± 0.042	3.783PheGly: 3.783 ± 0.062	0.79PheHis: 0.79 ± 0.025	1.83PheIle: 1.83 ± 0.042	1.027PheLys: 1.027 ± 0.03	3.692PheLeu: 3.692 ± 0.061	0.9PheMet: 0.9 ± 0.022	1.11PheAsn: 1.11 ± 0.032	1.655PhePro: 1.655 ± 0.036	1.126PheGln: 1.126 ± 0.031	2.262PheArg: 2.262 ± 0.037	2.513PheSer: 2.513 ± 0.042	1.995PheThr: 1.995 ± 0.037	2.941PheVal: 2.941 ± 0.054	0.574PheTrp: 0.574 ± 0.02	0.952PheTyr: 0.952 ± 0.027	0.001PheXaa: 0.001 ± 0.001
Gly	8.513GlyAla: 8.513 ± 0.088	0.797GlyCys: 0.797 ± 0.026	4.414GlyAsp: 4.414 ± 0.066	4.972GlyGlu: 4.972 ± 0.073	3.688GlyPhe: 3.688 ± 0.06	6.952GlyGly: 6.952 ± 0.092	1.856GlyHis: 1.856 ± 0.041	5.005GlyIle: 5.005 ± 0.077	3.324GlyLys: 3.324 ± 0.053	8.796GlyLeu: 8.796 ± 0.093	2.461GlyMet: 2.461 ± 0.05	2.201GlyAsn: 2.201 ± 0.046	3.301GlyPro: 3.301 ± 0.045	2.827GlyGln: 2.827 ± 0.048	5.897GlyArg: 5.897 ± 0.072	4.857GlySer: 4.857 ± 0.061	4.552GlyThr: 4.552 ± 0.06	5.889GlyVal: 5.889 ± 0.065	1.248GlyTrp: 1.248 ± 0.033	2.372GlyTyr: 2.372 ± 0.046	0.005GlyXaa: 0.005 ± 0.003
His	2.204HisAla: 2.204 ± 0.042	0.199HisCys: 0.199 ± 0.013	1.235HisAsp: 1.235 ± 0.031	1.084HisGlu: 1.084 ± 0.031	0.864HisPhe: 0.864 ± 0.03	1.892HisGly: 1.892 ± 0.038	0.603HisHis: 0.603 ± 0.027	0.996HisIle: 0.996 ± 0.025	0.496HisLys: 0.496 ± 0.019	2.095HisLeu: 2.095 ± 0.04	0.529HisMet: 0.529 ± 0.023	0.468HisAsn: 0.468 ± 0.02	1.318HisPro: 1.318 ± 0.03	0.64HisGln: 0.64 ± 0.023	1.41HisArg: 1.41 ± 0.03	0.969HisSer: 0.969 ± 0.025	0.778HisThr: 0.778 ± 0.027	1.552HisVal: 1.552 ± 0.035	0.285HisTrp: 0.285 ± 0.014	0.543HisTyr: 0.543 ± 0.021	0.001HisXaa: 0.001 ± 0.001
Ile	7.41IleAla: 7.41 ± 0.079	0.538IleCys: 0.538 ± 0.024	3.757IleAsp: 3.757 ± 0.053	3.637IleGlu: 3.637 ± 0.045	2.006IlePhe: 2.006 ± 0.041	5.296IleGly: 5.296 ± 0.077	0.977IleHis: 0.977 ± 0.026	2.744IleIle: 2.744 ± 0.052	1.459IleLys: 1.459 ± 0.032	5.011IleLeu: 5.011 ± 0.07	1.138IleMet: 1.138 ± 0.031	1.534IleAsn: 1.534 ± 0.032	2.44IlePro: 2.44 ± 0.043	1.337IleGln: 1.337 ± 0.036	3.564IleArg: 3.564 ± 0.052	3.255IleSer: 3.255 ± 0.048	2.81IleThr: 2.81 ± 0.048	4.508IleVal: 4.508 ± 0.067	0.626IleTrp: 0.626 ± 0.023	1.249IleTyr: 1.249 ± 0.036	0.003IleXaa: 0.003 ± 0.002
Lys	4.215LysAla: 4.215 ± 0.064	0.154LysCys: 0.154 ± 0.011	1.848LysAsp: 1.848 ± 0.043	1.73LysGlu: 1.73 ± 0.041	0.873LysPhe: 0.873 ± 0.024	2.608LysGly: 2.608 ± 0.039	0.635LysHis: 0.635 ± 0.022	1.884LysIle: 1.884 ± 0.04	1.316LysLys: 1.316 ± 0.039	3.251LysLeu: 3.251 ± 0.051	0.858LysMet: 0.858 ± 0.025	0.904LysAsn: 0.904 ± 0.025	2.0LysPro: 2.0 ± 0.043	1.016LysGln: 1.016 ± 0.03	2.261LysArg: 2.261 ± 0.043	1.905LysSer: 1.905 ± 0.038	2.133LysThr: 2.133 ± 0.05	2.481LysVal: 2.481 ± 0.047	0.35LysTrp: 0.35 ± 0.016	0.598LysTyr: 0.598 ± 0.023	0.004LysXaa: 0.004 ± 0.001
Leu	12.893LeuAla: 12.893 ± 0.135	0.874LeuCys: 0.874 ± 0.023	5.965LeuAsp: 5.965 ± 0.069	5.491LeuGlu: 5.491 ± 0.071	3.727LeuPhe: 3.727 ± 0.057	7.968LeuGly: 7.968 ± 0.101	1.794LeuHis: 1.794 ± 0.04	5.172LeuIle: 5.172 ± 0.07	3.737LeuLys: 3.737 ± 0.054	9.725LeuLeu: 9.725 ± 0.124	2.627LeuMet: 2.627 ± 0.051	2.55LeuAsn: 2.55 ± 0.046	5.367LeuPro: 5.367 ± 0.071	3.112LeuGln: 3.112 ± 0.052	6.339LeuArg: 6.339 ± 0.066	7.08LeuSer: 7.08 ± 0.105	5.704LeuThr: 5.704 ± 0.066	7.579LeuVal: 7.579 ± 0.096	1.102LeuTrp: 1.102 ± 0.031	2.123LeuTyr: 2.123 ± 0.043	0.007LeuXaa: 0.007 ± 0.003
Met	3.344MetAla: 3.344 ± 0.057	0.15MetCys: 0.15 ± 0.01	1.275MetAsp: 1.275 ± 0.03	1.373MetGlu: 1.373 ± 0.031	0.773MetPhe: 0.773 ± 0.025	1.911MetGly: 1.911 ± 0.042	0.455MetHis: 0.455 ± 0.018	1.669MetIle: 1.669 ± 0.038	1.087MetLys: 1.087 ± 0.031	2.716MetLeu: 2.716 ± 0.054	0.757MetMet: 0.757 ± 0.026	0.857MetAsn: 0.857 ± 0.026	1.583MetPro: 1.583 ± 0.031	0.908MetGln: 0.908 ± 0.029	1.834MetArg: 1.834 ± 0.04	1.831MetSer: 1.831 ± 0.035	2.043MetThr: 2.043 ± 0.036	1.869MetVal: 1.869 ± 0.035	0.199MetTrp: 0.199 ± 0.013	0.307MetTyr: 0.307 ± 0.013	0.002MetXaa: 0.002 ± 0.001
Asn	3.017AsnAla: 3.017 ± 0.043	0.238AsnCys: 0.238 ± 0.014	1.436AsnAsp: 1.436 ± 0.036	1.334AsnGlu: 1.334 ± 0.033	1.004AsnPhe: 1.004 ± 0.03	2.473AsnGly: 2.473 ± 0.046	0.552AsnHis: 0.552 ± 0.021	1.444AsnIle: 1.444 ± 0.033	0.735AsnLys: 0.735 ± 0.025	2.644AsnLeu: 2.644 ± 0.046	0.659AsnMet: 0.659 ± 0.023	0.767AsnAsn: 0.767 ± 0.025	1.81AsnPro: 1.81 ± 0.04	0.803AsnGln: 0.803 ± 0.027	1.906AsnArg: 1.906 ± 0.043	1.323AsnSer: 1.323 ± 0.031	1.283AsnThr: 1.283 ± 0.034	1.888AsnVal: 1.888 ± 0.036	0.439AsnTrp: 0.439 ± 0.02	0.647AsnTyr: 0.647 ± 0.024	0.001AsnXaa: 0.001 ± 0.001
Pro	5.772ProAla: 5.772 ± 0.083	0.265ProCys: 0.265 ± 0.015	3.496ProAsp: 3.496 ± 0.055	3.699ProGlu: 3.699 ± 0.057	2.043ProPhe: 2.043 ± 0.032	4.127ProGly: 4.127 ± 0.047	1.047ProHis: 1.047 ± 0.025	2.297ProIle: 2.297 ± 0.048	1.633ProLys: 1.633 ± 0.038	4.676ProLeu: 4.676 ± 0.057	1.245ProMet: 1.245 ± 0.03	1.261ProAsn: 1.261 ± 0.031	2.26ProPro: 2.26 ± 0.055	1.831ProGln: 1.831 ± 0.04	2.697ProArg: 2.697 ± 0.048	2.761ProSer: 2.761 ± 0.049	2.403ProThr: 2.403 ± 0.042	4.164ProVal: 4.164 ± 0.052	0.624ProTrp: 0.624 ± 0.022	1.194ProTyr: 1.194 ± 0.029	0.006ProXaa: 0.006 ± 0.003
Gln	4.091GlnAla: 4.091 ± 0.056	0.171GlnCys: 0.171 ± 0.01	1.609GlnAsp: 1.609 ± 0.036	1.894GlnGlu: 1.894 ± 0.035	1.116GlnPhe: 1.116 ± 0.026	2.346GlnGly: 2.346 ± 0.045	0.632GlnHis: 0.632 ± 0.021	1.933GlnIle: 1.933 ± 0.056	1.182GlnLys: 1.182 ± 0.032	2.912GlnLeu: 2.912 ± 0.054	1.044GlnMet: 1.044 ± 0.026	0.887GlnAsn: 0.887 ± 0.025	1.818GlnPro: 1.818 ± 0.039	1.326GlnGln: 1.326 ± 0.041	2.3GlnArg: 2.3 ± 0.048	1.929GlnSer: 1.929 ± 0.046	1.845GlnThr: 1.845 ± 0.042	2.356GlnVal: 2.356 ± 0.05	0.412GlnTrp: 0.412 ± 0.017	0.604GlnTyr: 0.604 ± 0.023	0.003GlnXaa: 0.003 ± 0.001
Arg	6.774ArgAla: 6.774 ± 0.077	0.454ArgCys: 0.454 ± 0.016	3.889ArgAsp: 3.889 ± 0.056	4.054ArgGlu: 4.054 ± 0.059	2.83ArgPhe: 2.83 ± 0.053	4.44ArgGly: 4.44 ± 0.061	1.761ArgHis: 1.761 ± 0.036	4.013ArgIle: 4.013 ± 0.057	2.344ArgLys: 2.344 ± 0.044	7.553ArgLeu: 7.553 ± 0.078	2.0ArgMet: 2.0 ± 0.036	1.963ArgAsn: 1.963 ± 0.039	3.281ArgPro: 3.281 ± 0.051	2.803ArgGln: 2.803 ± 0.047	5.337ArgArg: 5.337 ± 0.078	3.959ArgSer: 3.959 ± 0.065	3.301ArgThr: 3.301 ± 0.049	4.392ArgVal: 4.392 ± 0.06	0.871ArgTrp: 0.871 ± 0.024	1.735ArgTyr: 1.735 ± 0.037	0.008ArgXaa: 0.008 ± 0.003
Ser	6.31SerAla: 6.31 ± 0.073	0.428SerCys: 0.428 ± 0.021	3.129SerAsp: 3.129 ± 0.048	3.094SerGlu: 3.094 ± 0.052	2.502SerPhe: 2.502 ± 0.044	5.795SerGly: 5.795 ± 0.076	1.151SerHis: 1.151 ± 0.029	3.078SerIle: 3.078 ± 0.046	1.704SerLys: 1.704 ± 0.036	5.738SerLeu: 5.738 ± 0.076	1.503SerMet: 1.503 ± 0.037	1.421SerAsn: 1.421 ± 0.032	2.802SerPro: 2.802 ± 0.042	1.81SerGln: 1.81 ± 0.038	3.901SerArg: 3.901 ± 0.059	3.347SerSer: 3.347 ± 0.069	2.887SerThr: 2.887 ± 0.056	4.233SerVal: 4.233 ± 0.062	0.756SerTrp: 0.756 ± 0.023	1.451SerTyr: 1.451 ± 0.031	0.004SerXaa: 0.004 ± 0.002
Thr	6.312ThrAla: 6.312 ± 0.071	0.427ThrCys: 0.427 ± 0.019	2.888ThrAsp: 2.888 ± 0.056	2.904ThrGlu: 2.904 ± 0.047	2.044ThrPhe: 2.044 ± 0.039	5.054ThrGly: 5.054 ± 0.07	1.023ThrHis: 1.023 ± 0.026	3.106ThrIle: 3.106 ± 0.051	1.541ThrLys: 1.541 ± 0.034	5.715ThrLeu: 5.715 ± 0.07	1.365ThrMet: 1.365 ± 0.033	1.338ThrAsn: 1.338 ± 0.031	3.029ThrPro: 3.029 ± 0.052	1.469ThrGln: 1.469 ± 0.035	3.092ThrArg: 3.092 ± 0.046	3.042ThrSer: 3.042 ± 0.058	2.889ThrThr: 2.889 ± 0.049	4.627ThrVal: 4.627 ± 0.058	0.649ThrTrp: 0.649 ± 0.023	1.218ThrTyr: 1.218 ± 0.034	0.004ThrXaa: 0.004 ± 0.002
Val	9.039ValAla: 9.039 ± 0.092	0.629ValCys: 0.629 ± 0.025	4.23ValAsp: 4.23 ± 0.07	4.754ValGlu: 4.754 ± 0.065	2.885ValPhe: 2.885 ± 0.054	5.435ValGly: 5.435 ± 0.073	1.353ValHis: 1.353 ± 0.033	4.284ValIle: 4.284 ± 0.063	2.303ValLys: 2.303 ± 0.045	7.492ValLeu: 7.492 ± 0.092	1.982ValMet: 1.982 ± 0.036	2.025ValAsn: 2.025 ± 0.041	3.694ValPro: 3.694 ± 0.047	2.095ValGln: 2.095 ± 0.039	4.78ValArg: 4.78 ± 0.054	4.643ValSer: 4.643 ± 0.056	4.632ValThr: 4.632 ± 0.053	5.938ValVal: 5.938 ± 0.074	0.874ValTrp: 0.874 ± 0.029	1.547ValTyr: 1.547 ± 0.031	0.006ValXaa: 0.006 ± 0.002
Trp	1.12TrpAla: 1.12 ± 0.028	0.12TrpCys: 0.12 ± 0.01	0.593TrpAsp: 0.593 ± 0.024	0.584TrpGlu: 0.584 ± 0.02	0.534TrpPhe: 0.534 ± 0.021	0.8TrpGly: 0.8 ± 0.027	0.313TrpHis: 0.313 ± 0.014	0.71TrpIle: 0.71 ± 0.022	0.485TrpLys: 0.485 ± 0.019	1.548TrpLeu: 1.548 ± 0.042	0.406TrpMet: 0.406 ± 0.019	0.439TrpAsn: 0.439 ± 0.02	0.653TrpPro: 0.653 ± 0.025	0.612TrpGln: 0.612 ± 0.025	0.972TrpArg: 0.972 ± 0.026	0.832TrpSer: 0.832 ± 0.027	0.718TrpThr: 0.718 ± 0.025	0.725TrpVal: 0.725 ± 0.027	0.226TrpTrp: 0.226 ± 0.014	0.274TrpTyr: 0.274 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.469TyrAla: 2.469 ± 0.05	0.249TyrCys: 0.249 ± 0.013	1.479TyrAsp: 1.479 ± 0.036	1.299TyrGlu: 1.299 ± 0.033	0.955TyrPhe: 0.955 ± 0.03	2.103TyrGly: 2.103 ± 0.044	0.498TyrHis: 0.498 ± 0.018	0.986TyrIle: 0.986 ± 0.027	0.605TyrLys: 0.605 ± 0.024	2.34TyrLeu: 2.34 ± 0.044	0.454TyrMet: 0.454 ± 0.021	0.596TyrAsn: 0.596 ± 0.021	1.144TyrPro: 1.144 ± 0.033	0.763TyrGln: 0.763 ± 0.026	1.784TyrArg: 1.784 ± 0.043	1.226TyrSer: 1.226 ± 0.033	1.141TyrThr: 1.141 ± 0.032	1.712TyrVal: 1.712 ± 0.037	0.352TyrTrp: 0.352 ± 0.017	0.658TyrTyr: 0.658 ± 0.024	0.003TyrXaa: 0.003 ± 0.001
Xaa	0.01XaaAla: 0.01 ± 0.004	0.001XaaCys: 0.001 ± 0.001	0.004XaaAsp: 0.004 ± 0.002	0.007XaaGlu: 0.007 ± 0.003	0.002XaaPhe: 0.002 ± 0.001	0.009XaaGly: 0.009 ± 0.003	0.003XaaHis: 0.003 ± 0.002	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.008XaaLeu: 0.008 ± 0.003	0.001XaaMet: 0.001 ± 0.001	0.002XaaAsn: 0.002 ± 0.001	0.004XaaPro: 0.004 ± 0.002	0.002XaaGln: 0.002 ± 0.001	0.007XaaArg: 0.007 ± 0.003	0.004XaaSer: 0.004 ± 0.002	0.001XaaThr: 0.001 ± 0.001	0.006XaaVal: 0.006 ± 0.003	0.001XaaTrp: 0.001 ± 0.001	0.003XaaTyr: 0.003 ± 0.002	0.012XaaXaa: 0.012 ± 0.004

Statistics based on 4417 proteins (1347192 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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