Raccoon polyomavirus
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J7FZP3|J7FZP3_9POLY Small T antigen OS=Raccoon polyomavirus OX=1219896 PE=4 SV=1
MM1 pKa = 7.61 GGFLSLFLSLGEE13 pKa = 4.12 IVAEE17 pKa = 4.54 LSATTGIAVEE27 pKa = 4.59 SILTGEE33 pKa = 4.08 ALAAIEE39 pKa = 4.54 AEE41 pKa = 4.55 VGSLMTVEE49 pKa = 4.24 GLSGIEE55 pKa = 4.24 ALSQLGFTAEE65 pKa = 3.99 QFSNFSLVSSLVNQAAGYY83 pKa = 10.27 GVFAQTLSGISALVGAGIKK102 pKa = 10.05 LALDD106 pKa = 3.54 EE107 pKa = 4.36 QSTVNHH113 pKa = 6.32 NLYY116 pKa = 10.61 SLSAAKK122 pKa = 10.27 RR123 pKa = 11.84 EE124 pKa = 4.06 EE125 pKa = 3.82 EE126 pKa = 3.73 RR127 pKa = 11.84 LVNNGIQKK135 pKa = 10.3 LSWSFGLDD143 pKa = 3.29 PLNWDD148 pKa = 3.11 QSLLHH153 pKa = 6.59 AVGKK157 pKa = 8.74 SVWEE161 pKa = 4.2 GLQKK165 pKa = 10.8 HH166 pKa = 6.3 PGVNHH171 pKa = 6.27 VYY173 pKa = 11.03 SNLEE177 pKa = 3.55 ALLRR181 pKa = 11.84 QARR184 pKa = 11.84 WVVKK188 pKa = 10.49 RR189 pKa = 11.84 EE190 pKa = 4.06 STSALPHH197 pKa = 5.85 QIGEE201 pKa = 4.29 VIHH204 pKa = 6.24 FFEE207 pKa = 4.89 SHH209 pKa = 4.13 VHH211 pKa = 5.79 SNGVPDD217 pKa = 3.82 WLLPLILGLKK227 pKa = 9.78 GDD229 pKa = 4.26 PTPAWHH235 pKa = 6.77 HH236 pKa = 6.66 LEE238 pKa = 4.89 DD239 pKa = 4.73 GPP241 pKa = 5.13
Molecular weight: 25.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.028
IPC2_protein 5.016
IPC_protein 4.952
Toseland 4.991
ProMoST 5.105
Dawson 4.965
Bjellqvist 5.08
Wikipedia 4.825
Rodwell 4.914
Grimsley 4.927
Solomon 4.965
Lehninger 4.927
Nozaki 5.105
DTASelect 5.194
Thurlkill 4.991
EMBOSS 4.889
Sillero 5.194
Patrickios 3.999
IPC_peptide 4.978
IPC2_peptide 5.194
IPC2.peptide.svr19 5.107
Protein with the highest isoelectric point:
>tr|J7G293|J7G293_9POLY Capsid protein VP1 OS=Raccoon polyomavirus OX=1219896 PE=3 SV=1
MM1 pKa = 7.61 DD2 pKa = 3.8 TVLTSSEE9 pKa = 3.81 RR10 pKa = 11.84 RR11 pKa = 11.84 EE12 pKa = 4.07 LCSLLEE18 pKa = 5.16 LDD20 pKa = 3.79 PQLWGNIPVMRR31 pKa = 11.84 NCYY34 pKa = 9.85 KK35 pKa = 10.56 KK36 pKa = 10.66 SCLKK40 pKa = 9.51 YY41 pKa = 10.58 HH42 pKa = 7.1 PDD44 pKa = 4.22 KK45 pKa = 11.65 GGDD48 pKa = 3.52 GQKK51 pKa = 9.18 MVRR54 pKa = 11.84 LNEE57 pKa = 3.53 LWARR61 pKa = 11.84 ATEE64 pKa = 3.93 NLTRR68 pKa = 11.84 LRR70 pKa = 11.84 EE71 pKa = 4.15 DD72 pKa = 3.64 CGFQVSDD79 pKa = 4.57 PLEE82 pKa = 4.52 SSPKK86 pKa = 9.86 TLMAHH91 pKa = 7.39 LGSTWKK97 pKa = 10.76 SKK99 pKa = 9.5 FCKK102 pKa = 10.61 GPTCRR107 pKa = 11.84 INMKK111 pKa = 9.52 PAYY114 pKa = 8.95 CSCITCRR121 pKa = 11.84 LYY123 pKa = 10.72 KK124 pKa = 9.79 QHH126 pKa = 6.58 QAIKK130 pKa = 9.22 KK131 pKa = 8.1 KK132 pKa = 10.25 KK133 pKa = 9.61 KK134 pKa = 9.54 RR135 pKa = 11.84 RR136 pKa = 11.84 CLVWGQCFCFSCYY149 pKa = 10.08 LHH151 pKa = 6.89 WFGFPNTWCFFDD163 pKa = 2.76 WWMTLILEE171 pKa = 4.46 TDD173 pKa = 3.75 LNLLNLKK180 pKa = 8.94 FCKK183 pKa = 10.57 YY184 pKa = 10.2 EE185 pKa = 3.71 FLNIKK190 pKa = 9.42 NFPYY194 pKa = 10.63 QNASLMTFSLQDD206 pKa = 3.31 EE207 pKa = 4.47 PPIYY211 pKa = 8.89 GTPAFRR217 pKa = 11.84 EE218 pKa = 3.82 WWFYY222 pKa = 8.63 QQNLYY227 pKa = 8.28 TWSHH231 pKa = 7.2 DD232 pKa = 3.76 GPSTSSSSGRR242 pKa = 11.84 SRR244 pKa = 11.84 RR245 pKa = 11.84 WDD247 pKa = 3.09 SSRR250 pKa = 11.84 DD251 pKa = 3.33 HH252 pKa = 7.56 GEE254 pKa = 3.7 FAGGEE259 pKa = 4.31 GSSGRR264 pKa = 11.84 DD265 pKa = 2.49 RR266 pKa = 11.84 VHH268 pKa = 6.27 TSKK271 pKa = 10.91 SSTSMPSHH279 pKa = 6.75 RR280 pKa = 11.84 PHH282 pKa = 7.61 SSANLHH288 pKa = 6.43 GDD290 pKa = 3.19 NSEE293 pKa = 4.76 DD294 pKa = 3.56 PLFCEE299 pKa = 4.55 EE300 pKa = 4.79 TLDD303 pKa = 4.59 SSPSPPPSPAADD315 pKa = 3.29 PHH317 pKa = 6.39 TFTSSQSSTPSSAFGFSSQTSTPSNASQGDD347 pKa = 3.73 SRR349 pKa = 11.84 TTGPHH354 pKa = 6.82 PNTPSPRR361 pKa = 11.84 RR362 pKa = 11.84 RR363 pKa = 11.84 KK364 pKa = 9.68 HH365 pKa = 6.68 RR366 pKa = 11.84 EE367 pKa = 3.62 DD368 pKa = 4.32 HH369 pKa = 6.03 PTSPYY374 pKa = 10.89 ASGGSFSSTPPKK386 pKa = 10.22 SKK388 pKa = 10.48 KK389 pKa = 9.75 PRR391 pKa = 11.84 DD392 pKa = 3.68 SFSPLDD398 pKa = 3.86 FPSDD402 pKa = 3.92 LLDD405 pKa = 3.97 CLSRR409 pKa = 11.84 ATLSNKK415 pKa = 6.74 TQNAFLIFSTKK426 pKa = 10.25 EE427 pKa = 4.09 KK428 pKa = 11.02 VCLLYY433 pKa = 10.96 DD434 pKa = 3.69 KK435 pKa = 10.93 TDD437 pKa = 3.34 DD438 pKa = 3.64 KK439 pKa = 11.77 YY440 pKa = 11.15 KK441 pKa = 10.97 VEE443 pKa = 4.75 FKK445 pKa = 10.83 SLHH448 pKa = 5.95 KK449 pKa = 10.4 YY450 pKa = 10.31 KK451 pKa = 10.69 NGSGFLFIILLTKK464 pKa = 10.51 HH465 pKa = 5.81 RR466 pKa = 11.84 VTAIKK471 pKa = 10.31 NFCAQFCTISFLLCKK486 pKa = 10.32 AVIQPVQCYY495 pKa = 10.44 DD496 pKa = 3.88 RR497 pKa = 11.84 LCKK500 pKa = 10.45 DD501 pKa = 3.5 PYY503 pKa = 11.04 TFLKK507 pKa = 10.72 ANKK510 pKa = 9.24 CGIFEE515 pKa = 4.87 GEE517 pKa = 4.05 FAEE520 pKa = 5.58 QKK522 pKa = 11.05 DD523 pKa = 4.08 PTCDD527 pKa = 2.81 WNQIADD533 pKa = 3.69 FALAHH538 pKa = 6.57 LLDD541 pKa = 5.23 DD542 pKa = 4.75 PLLILAHH549 pKa = 6.32 YY550 pKa = 10.43 LDD552 pKa = 4.75 FSKK555 pKa = 11.0 PPGNCTKK562 pKa = 10.69 CLTGKK567 pKa = 9.6 GLKK570 pKa = 9.81 AHH572 pKa = 7.12 ADD574 pKa = 3.28 HH575 pKa = 7.36 AKK577 pKa = 10.06 EE578 pKa = 3.97 HH579 pKa = 7.27 DD580 pKa = 3.61 NAKK583 pKa = 10.59 LFIKK587 pKa = 10.53 SKK589 pKa = 7.7 SQKK592 pKa = 10.37 SICQQAADD600 pKa = 3.91 VVCAKK605 pKa = 10.26 KK606 pKa = 10.33 RR607 pKa = 11.84 LAMLEE612 pKa = 3.9 QTRR615 pKa = 11.84 TEE617 pKa = 3.83 MLEE620 pKa = 3.91 EE621 pKa = 4.21 KK622 pKa = 10.06 FRR624 pKa = 11.84 KK625 pKa = 9.03 QLAILKK631 pKa = 8.53 EE632 pKa = 4.01 LEE634 pKa = 4.42 VIDD637 pKa = 4.12 LHH639 pKa = 7.72 LILGGVAWYY648 pKa = 10.89 SVMFQNFADD657 pKa = 3.85 MLKK660 pKa = 10.99 GLLQLFTDD668 pKa = 5.11 NIPKK672 pKa = 9.87 KK673 pKa = 10.48 RR674 pKa = 11.84 NALFIGPINSGKK686 pKa = 9.01 TSFAAALIDD695 pKa = 4.06 LLEE698 pKa = 4.55 GKK700 pKa = 9.48 ALNINCPADD709 pKa = 3.44 KK710 pKa = 11.2 LNFEE714 pKa = 5.12 LGCALDD720 pKa = 4.22 LFMVCFEE727 pKa = 4.54 DD728 pKa = 4.66 VKK730 pKa = 11.12 GSKK733 pKa = 10.18 CSNKK737 pKa = 9.93 DD738 pKa = 3.46 LQSGQGMNNLDD749 pKa = 3.96 NLRR752 pKa = 11.84 DD753 pKa = 3.82 HH754 pKa = 7.19 MDD756 pKa = 3.48 GAVPVQLEE764 pKa = 4.09 RR765 pKa = 11.84 KK766 pKa = 7.96 HH767 pKa = 5.02 QNKK770 pKa = 9.65 RR771 pKa = 11.84 SQIFPPCIVTCNEE784 pKa = 3.8 YY785 pKa = 10.82 YY786 pKa = 10.22 IPGTVATRR794 pKa = 11.84 FAQTLTFAIKK804 pKa = 10.53 LCLKK808 pKa = 10.77 NSIEE812 pKa = 4.62 KK813 pKa = 10.31 NILMQQKK820 pKa = 9.41 RR821 pKa = 11.84 VLQRR825 pKa = 11.84 GCTLLMALIWLLPLDD840 pKa = 4.91 DD841 pKa = 4.97 FSDD844 pKa = 5.29 GIRR847 pKa = 11.84 SDD849 pKa = 3.65 VKK851 pKa = 10.53 DD852 pKa = 3.94 FKK854 pKa = 11.38 GAVEE858 pKa = 4.68 GDD860 pKa = 4.03 VGWATYY866 pKa = 10.76 QKK868 pKa = 9.69 MVEE871 pKa = 4.15 NVEE874 pKa = 4.54 NGDD877 pKa = 4.04 DD878 pKa = 3.7 PMKK881 pKa = 11.03 GILEE885 pKa = 4.03 QDD887 pKa = 3.35 NEE889 pKa = 4.16 QDD891 pKa = 4.13 NEE893 pKa = 4.29 DD894 pKa = 3.62 VDD896 pKa = 4.31 SGRR899 pKa = 11.84 FTQQ902 pKa = 4.71
Molecular weight: 102.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.107
IPC2_protein 7.468
IPC_protein 7.22
Toseland 6.839
ProMoST 7.878
Dawson 7.951
Bjellqvist 8.463
Wikipedia 7.849
Rodwell 7.98
Grimsley 6.883
Solomon 7.98
Lehninger 7.995
Nozaki 8.814
DTASelect 8.112
Thurlkill 8.185
EMBOSS 8.17
Sillero 8.551
Patrickios 4.291
IPC_peptide 7.966
IPC2_peptide 7.497
IPC2.peptide.svr19 7.417
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2496
196
902
499.2
55.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.849 ± 0.571
2.965 ± 0.668
6.13 ± 0.718
5.369 ± 0.517
4.728 ± 0.579
6.771 ± 0.801
2.845 ± 0.338
4.327 ± 0.473
7.011 ± 0.765
10.617 ± 0.797
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.963 ± 0.267
4.607 ± 0.126
5.769 ± 0.605
4.207 ± 0.226
4.006 ± 0.415
8.534 ± 0.695
5.529 ± 0.446
4.728 ± 1.06
1.683 ± 0.216
2.364 ± 0.354
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here