Umboniibacter marinipuniceus
Average proteome isoelectric point is 5.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2413 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3M0A976|A0A3M0A976_9GAMM Glycerate dehydrogenase OS=Umboniibacter marinipuniceus OX=569599 GN=DFR27_1405 PE=3 SV=1
MM1 pKa = 7.34 EE2 pKa = 5.86 RR3 pKa = 11.84 SLDD6 pKa = 3.56 SRR8 pKa = 11.84 LVIVSALLALTMSAVTSAQDD28 pKa = 3.7 SEE30 pKa = 4.65 IEE32 pKa = 4.23 SSAVAAQSEE41 pKa = 5.08 STTEE45 pKa = 4.05 TEE47 pKa = 4.26 AEE49 pKa = 4.25 LPAVTYY55 pKa = 9.81 EE56 pKa = 4.41 SYY58 pKa = 11.2 GSDD61 pKa = 2.82 VGFVQTQPVPYY72 pKa = 10.22 DD73 pKa = 3.57 GDD75 pKa = 3.66 PFEE78 pKa = 5.06 DD79 pKa = 3.9 LNRR82 pKa = 11.84 AMLTFNDD89 pKa = 3.32 VADD92 pKa = 5.14 RR93 pKa = 11.84 YY94 pKa = 10.54 LLLPIVTGYY103 pKa = 11.05 KK104 pKa = 10.39 YY105 pKa = 9.27 ITPDD109 pKa = 3.23 PVEE112 pKa = 4.34 RR113 pKa = 11.84 SVDD116 pKa = 3.65 NIFANLDD123 pKa = 4.28 DD124 pKa = 4.86 IGSAANSILQLKK136 pKa = 9.88 IGDD139 pKa = 3.74 AGVYY143 pKa = 10.03 AGRR146 pKa = 11.84 FLTNTTIGVLGIWDD160 pKa = 3.78 VASEE164 pKa = 3.98 LGMRR168 pKa = 11.84 RR169 pKa = 11.84 LEE171 pKa = 4.52 GEE173 pKa = 4.75 DD174 pKa = 3.34 FGQTLGYY181 pKa = 10.08 YY182 pKa = 9.04 GVPEE186 pKa = 4.73 GPYY189 pKa = 11.02 LMLPFYY195 pKa = 10.87 GPSTLRR201 pKa = 11.84 DD202 pKa = 3.21 APARR206 pKa = 11.84 YY207 pKa = 9.15 VDD209 pKa = 3.73 SFVDD213 pKa = 3.42 YY214 pKa = 10.13 TSYY217 pKa = 11.21 VDD219 pKa = 4.7 HH220 pKa = 6.7 VPTRR224 pKa = 11.84 NSLMGVEE231 pKa = 4.21 AVNVRR236 pKa = 11.84 SQLIQAEE243 pKa = 4.16 AFITGDD249 pKa = 3.02 RR250 pKa = 11.84 YY251 pKa = 9.04 TFIRR255 pKa = 11.84 DD256 pKa = 2.95 AYY258 pKa = 8.84 LQRR261 pKa = 11.84 RR262 pKa = 11.84 QYY264 pKa = 11.26 LVLDD268 pKa = 3.83 GQMPDD273 pKa = 4.27 DD274 pKa = 4.28 EE275 pKa = 5.06 EE276 pKa = 5.16 FDD278 pKa = 3.66 EE279 pKa = 5.51 FGGFGSAGEE288 pKa = 4.42 SYY290 pKa = 10.86 GGEE293 pKa = 4.26 SYY295 pKa = 11.02 GGEE298 pKa = 4.3 SYY300 pKa = 11.02 GGEE303 pKa = 4.3 SYY305 pKa = 11.02 GGEE308 pKa = 4.3 SYY310 pKa = 11.02 GGEE313 pKa = 4.3 SYY315 pKa = 11.02 GGEE318 pKa = 4.17 SYY320 pKa = 11.25 GGDD323 pKa = 3.41 EE324 pKa = 5.42 DD325 pKa = 5.59 SDD327 pKa = 4.19 PNRR330 pKa = 11.84 DD331 pKa = 3.81 NLSGSADD338 pKa = 3.81 DD339 pKa = 6.01 QIDD342 pKa = 3.87 PPLNEE347 pKa = 3.72 TDD349 pKa = 3.47 GRR351 pKa = 11.84 SDD353 pKa = 4.17 NDD355 pKa = 3.74 DD356 pKa = 4.45 KK357 pKa = 11.65 LPQDD361 pKa = 3.84 SEE363 pKa = 4.47 FF364 pKa = 3.99
Molecular weight: 39.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.757
IPC_protein 3.783
Toseland 3.567
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.681
Rodwell 3.605
Grimsley 3.465
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.101
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.897
Patrickios 1.049
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.794
Protein with the highest isoelectric point:
>tr|A0A3M0AB83|A0A3M0AB83_9GAMM Glycine dehydrogenase (decarboxylating) OS=Umboniibacter marinipuniceus OX=569599 GN=gcvP PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 7.97 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATANGRR28 pKa = 11.84 QVINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.39 GRR39 pKa = 11.84 KK40 pKa = 8.88 RR41 pKa = 11.84 LTVV44 pKa = 3.11
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2413
0
2413
804001
29
1961
333.2
36.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.053 ± 0.054
0.969 ± 0.014
5.779 ± 0.045
6.437 ± 0.05
3.986 ± 0.031
7.251 ± 0.044
2.128 ± 0.023
5.822 ± 0.037
3.818 ± 0.039
10.335 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.369 ± 0.022
3.81 ± 0.027
4.023 ± 0.026
4.179 ± 0.031
5.383 ± 0.041
6.915 ± 0.043
5.205 ± 0.037
7.331 ± 0.037
1.337 ± 0.022
2.869 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here