Proteus phage vB_PmiP_RS51pmB

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514CYG9|A0A514CYG9_9CAUD Uncharacterized protein OS=Proteus phage vB_PmiP_RS51pmB OX=2250313 PE=4 SV=1
MM1 pKa = 7.09KK2 pKa = 9.75TITYY6 pKa = 9.99RR7 pKa = 11.84GEE9 pKa = 3.92QYY11 pKa = 10.6EE12 pKa = 4.25VEE14 pKa = 4.76DD15 pKa = 3.68WVNWVAMDD23 pKa = 4.25AGGEE27 pKa = 4.0IYY29 pKa = 10.05AYY31 pKa = 7.96EE32 pKa = 4.32HH33 pKa = 6.5EE34 pKa = 5.55PIVSSEE40 pKa = 3.99NDD42 pKa = 3.59WINTGGNCFWIGSEE56 pKa = 3.94PQDD59 pKa = 2.97WDD61 pKa = 3.38EE62 pKa = 4.48SLEE65 pKa = 4.23KK66 pKa = 10.98VV67 pKa = 3.47

Molecular weight: 7.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514CYF8|A0A514CYF8_9CAUD Uncharacterized protein OS=Proteus phage vB_PmiP_RS51pmB OX=2250313 PE=4 SV=1
MM1 pKa = 7.36NGVISNRR8 pKa = 11.84LSRR11 pKa = 11.84VGLFPFEE18 pKa = 4.47LCNGHH23 pKa = 7.41LLINDD28 pKa = 3.37NGKK31 pKa = 10.24APDD34 pKa = 3.62HH35 pKa = 6.28KK36 pKa = 10.42HH37 pKa = 6.05RR38 pKa = 11.84SHH40 pKa = 7.74KK41 pKa = 8.9ITPVHH46 pKa = 6.29NLLLRR51 pKa = 11.84KK52 pKa = 9.78SFLMHH57 pKa = 5.23QAQYY61 pKa = 10.19RR62 pKa = 11.84SVMRR66 pKa = 11.84TTFYY70 pKa = 10.04SQSYY74 pKa = 8.01FAPP77 pKa = 4.23

Molecular weight: 8.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

13107

43

786

157.9

17.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.874 ± 0.421

1.427 ± 0.141

6.973 ± 0.238

6.569 ± 0.292

3.883 ± 0.206

7.691 ± 0.242

1.701 ± 0.136

6.973 ± 0.226

7.187 ± 0.231

6.691 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.548 ± 0.199

4.746 ± 0.184

3.212 ± 0.204

3.136 ± 0.15

4.234 ± 0.182

6.226 ± 0.196

5.432 ± 0.23

7.233 ± 0.23

1.427 ± 0.117

3.838 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski