Kundal virus
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A499RSG4|A0A499RSG4_9REOV RNA-dependent RNA polymerase OS=Kundal virus OX=2290890 GN=VP1 PE=4 SV=1
MM1 pKa = 7.68 TIDD4 pKa = 3.92 QNDD7 pKa = 3.85 PDD9 pKa = 3.94 VFQTVIEE16 pKa = 4.37 TKK18 pKa = 10.75 FIVSCLNFINLSLTEE33 pKa = 4.07 GWVLEE38 pKa = 4.45 HH39 pKa = 7.12 KK40 pKa = 11.11 SNFYY44 pKa = 11.08 LLYY47 pKa = 9.15 PYY49 pKa = 10.12 EE50 pKa = 5.74 AGAVHH55 pKa = 6.98 FPEE58 pKa = 4.39 LQAIGVQLVQIDD70 pKa = 3.95 GLQDD74 pKa = 3.18 VTNFLSRR81 pKa = 11.84 LGDD84 pKa = 3.51 NPRR87 pKa = 11.84 LILHH91 pKa = 6.3 YY92 pKa = 9.74 VQQNAEE98 pKa = 4.01 RR99 pKa = 11.84 LTGVVHH105 pKa = 5.75 QRR107 pKa = 11.84 NANGIYY113 pKa = 10.73 EE114 pKa = 4.11 MMLVKK119 pKa = 10.49 RR120 pKa = 11.84 SSGQIIKK127 pKa = 9.86 PIQEE131 pKa = 4.21 LVSLYY136 pKa = 10.56 SGRR139 pKa = 11.84 QPTEE143 pKa = 3.55 RR144 pKa = 11.84 DD145 pKa = 3.42 EE146 pKa = 5.49 NEE148 pKa = 5.19 DD149 pKa = 3.81 LAQDD153 pKa = 3.89 LQLADD158 pKa = 3.78 DD159 pKa = 4.6 AEE161 pKa = 4.72 FNLRR165 pKa = 11.84 PDD167 pKa = 4.14 EE168 pKa = 4.57 SPSIPVIEE176 pKa = 4.47 GSKK179 pKa = 11.02 DD180 pKa = 2.93 VDD182 pKa = 3.88 LPLSQEE188 pKa = 4.32 DD189 pKa = 4.32 EE190 pKa = 4.08 ISLEE194 pKa = 4.32 GIEE197 pKa = 5.03 EE198 pKa = 3.85 ILNRR202 pKa = 11.84 LKK204 pKa = 11.2 GEE206 pKa = 4.02 EE207 pKa = 4.25 GKK209 pKa = 10.83 SGVSLPKK216 pKa = 9.83 EE217 pKa = 4.51 LIRR220 pKa = 11.84 KK221 pKa = 9.25 EE222 pKa = 4.11 EE223 pKa = 4.59 GEE225 pKa = 4.79 DD226 pKa = 3.28 IPPVIEE232 pKa = 3.64 KK233 pKa = 10.58 RR234 pKa = 11.84 KK235 pKa = 8.72 IRR237 pKa = 11.84 RR238 pKa = 11.84 INRR241 pKa = 11.84 RR242 pKa = 11.84 PPPLDD247 pKa = 3.99 RR248 pKa = 11.84 DD249 pKa = 4.05 LVQLDD254 pKa = 3.37 LAGRR258 pKa = 11.84 DD259 pKa = 4.01 FIQALRR265 pKa = 11.84 EE266 pKa = 4.04 AYY268 pKa = 10.67 NEE270 pKa = 3.94 VEE272 pKa = 4.11 QFDD275 pKa = 4.09 DD276 pKa = 5.21 GEE278 pKa = 4.68 INVDD282 pKa = 3.19 NLPNFRR288 pKa = 11.84 RR289 pKa = 11.84 AFNLVEE295 pKa = 4.4 TYY297 pKa = 10.88 VIDD300 pKa = 3.54 HH301 pKa = 6.46 TSKK304 pKa = 10.5 FDD306 pKa = 3.73 PYY308 pKa = 10.74 RR309 pKa = 11.84 LPSSNYY315 pKa = 8.13 NQNIVEE321 pKa = 4.1 NFEE324 pKa = 4.3 VVIGDD329 pKa = 3.58 TRR331 pKa = 11.84 EE332 pKa = 3.89 SLRR335 pKa = 11.84 DD336 pKa = 3.42 SGEE339 pKa = 3.94 GLDD342 pKa = 3.71 ILHH345 pKa = 6.7 IPGVEE350 pKa = 3.95 GEE352 pKa = 4.53 YY353 pKa = 10.9 LDD355 pKa = 5.61 DD356 pKa = 6.12 LNLLEE361 pKa = 4.04 QRR363 pKa = 11.84 QIQEE367 pKa = 3.77 EE368 pKa = 4.33 SQQIEE373 pKa = 4.55 FQLGDD378 pKa = 3.43 YY379 pKa = 10.8 GFVIGHH385 pKa = 7.47 DD386 pKa = 3.68 GTLQKK391 pKa = 10.2 IDD393 pKa = 3.41 VHH395 pKa = 5.2 NQKK398 pKa = 10.07 ARR400 pKa = 11.84 QLRR403 pKa = 11.84 GEE405 pKa = 4.33 LIEE408 pKa = 4.42 PFVEE412 pKa = 3.85 NLHH415 pKa = 5.4 FQWRR419 pKa = 11.84 GNATFIPVRR428 pKa = 11.84 RR429 pKa = 11.84 FLEE432 pKa = 4.22 RR433 pKa = 11.84 FFSHH437 pKa = 7.12 PPGVEE442 pKa = 3.79 DD443 pKa = 4.21 GLFSVGRR450 pKa = 11.84 ILRR453 pKa = 11.84 DD454 pKa = 3.35 LQTTEE459 pKa = 3.24 ISYY462 pKa = 8.16 NTVDD466 pKa = 3.17 STRR469 pKa = 11.84 RR470 pKa = 11.84 NVPANCLNLDD480 pKa = 4.12 NMNDD484 pKa = 3.51 TCRR487 pKa = 11.84 HH488 pKa = 5.53 LFLMLRR494 pKa = 11.84 RR495 pKa = 11.84 WVTLARR501 pKa = 11.84 RR502 pKa = 11.84 FQNTALHH509 pKa = 6.38 KK510 pKa = 10.18 NVAMEE515 pKa = 4.2 LARR518 pKa = 11.84 VYY520 pKa = 10.75 GGWLPGVQYY529 pKa = 8.44 TIPSNVIRR537 pKa = 11.84 LFRR540 pKa = 11.84 NGRR543 pKa = 11.84 LLNHH547 pKa = 6.7 INAPACYY554 pKa = 9.53 SRR556 pKa = 11.84 LHH558 pKa = 5.78 MLIDD562 pKa = 3.5 QPLIGVADD570 pKa = 3.94 PRR572 pKa = 11.84 DD573 pKa = 3.84 CVKK576 pKa = 10.95 DD577 pKa = 4.11 FARR580 pKa = 11.84 SEE582 pKa = 3.6 EE583 pKa = 4.11 WIYY586 pKa = 10.63 IDD588 pKa = 5.18 NYY590 pKa = 10.67 LPYY593 pKa = 9.84 ILHH596 pKa = 6.03 IEE598 pKa = 4.15 FWEE601 pKa = 4.12 MFINWFPDD609 pKa = 2.98 CGMEE613 pKa = 5.02 GIQRR617 pKa = 11.84 LSPIFPTQLSDD628 pKa = 3.72 RR629 pKa = 11.84 EE630 pKa = 3.72 IWIAARR636 pKa = 11.84 LLL638 pKa = 3.85
Molecular weight: 73.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.843
IPC2_protein 4.825
IPC_protein 4.787
Toseland 4.673
ProMoST 4.902
Dawson 4.749
Bjellqvist 4.889
Wikipedia 4.609
Rodwell 4.647
Grimsley 4.584
Solomon 4.749
Lehninger 4.698
Nozaki 4.863
DTASelect 5.016
Thurlkill 4.66
EMBOSS 4.635
Sillero 4.927
Patrickios 4.037
IPC_peptide 4.762
IPC2_peptide 4.927
IPC2.peptide.svr19 4.873
Protein with the highest isoelectric point:
>tr|A0A499RIH4|A0A499RIH4_9REOV VP3 OS=Kundal virus OX=2290890 GN=VP3 PE=4 SV=1
MM1 pKa = 7.59 MLRR4 pKa = 11.84 QPVKK8 pKa = 10.88 YY9 pKa = 10.52 NIFLLPTGRR18 pKa = 11.84 KK19 pKa = 8.97 LLSKK23 pKa = 8.61 TKK25 pKa = 10.46 QYY27 pKa = 8.85 GTLDD31 pKa = 3.83 RR32 pKa = 11.84 LPLEE36 pKa = 3.96 DD37 pKa = 3.15 WKK39 pKa = 10.88 IYY41 pKa = 9.6 EE42 pKa = 4.75 AIEE45 pKa = 4.25 NKK47 pKa = 9.86 LKK49 pKa = 10.86 YY50 pKa = 10.1 AIHH53 pKa = 7.11 HH54 pKa = 6.24 YY55 pKa = 10.26 PFDD58 pKa = 3.2 WRR60 pKa = 11.84 QRR62 pKa = 11.84 ITFHH66 pKa = 6.57 EE67 pKa = 4.36 KK68 pKa = 10.49 NGFKK72 pKa = 9.87 MAILVPNSMLLSDD85 pKa = 4.01 YY86 pKa = 10.82 KK87 pKa = 11.09 EE88 pKa = 4.46 SIKK91 pKa = 10.61 QVITSLGHH99 pKa = 5.81 RR100 pKa = 11.84 PSDD103 pKa = 3.19 IMFVDD108 pKa = 3.49 KK109 pKa = 11.24 ANYY112 pKa = 7.15 TKK114 pKa = 10.43 RR115 pKa = 11.84 HH116 pKa = 4.34 KK117 pKa = 10.07 HH118 pKa = 4.63 GKK120 pKa = 8.46 IVIYY124 pKa = 10.49 LDD126 pKa = 3.34 TEE128 pKa = 3.88 MDD130 pKa = 3.61 LGIDD134 pKa = 3.73 GFKK137 pKa = 10.54 VDD139 pKa = 3.37 SSKK142 pKa = 11.41 YY143 pKa = 10.41 DD144 pKa = 3.28 STKK147 pKa = 9.41 MGKK150 pKa = 9.1 ILGAMLQSKK159 pKa = 8.07 LLCSDD164 pKa = 4.83 FIKK167 pKa = 10.55 PINGCKK173 pKa = 10.27 YY174 pKa = 9.84 EE175 pKa = 4.2 DD176 pKa = 3.74 VGNLLPQLLEE186 pKa = 3.74 KK187 pKa = 10.77 DD188 pKa = 3.07 RR189 pKa = 11.84 WILSVQANYY198 pKa = 9.66 HH199 pKa = 5.34 HH200 pKa = 7.15 VYY202 pKa = 10.55 GRR204 pKa = 11.84 TRR206 pKa = 11.84 SIEE209 pKa = 3.76 VGMVEE214 pKa = 5.79 DD215 pKa = 5.31 FFASEE220 pKa = 4.67 GVCYY224 pKa = 8.96 TVKK227 pKa = 10.43 DD228 pKa = 3.06 WHH230 pKa = 6.61 GAVKK234 pKa = 10.86 YY235 pKa = 9.65 EE236 pKa = 4.35 GSIDD240 pKa = 3.81 SFNGRR245 pKa = 11.84 LMLLPSTILTPSEE258 pKa = 4.37 YY259 pKa = 10.17 EE260 pKa = 3.87 RR261 pKa = 11.84 QTALSLRR268 pKa = 11.84 MKK270 pKa = 10.06 KK271 pKa = 10.16 SKK273 pKa = 10.89 NILSAFMYY281 pKa = 9.11 ITFQLKK287 pKa = 9.52 IDD289 pKa = 4.53 PYY291 pKa = 11.31 SLAQIPGLSHH301 pKa = 6.39 NDD303 pKa = 3.16 KK304 pKa = 10.87 YY305 pKa = 11.63 SVIVPTGTPPPRR317 pKa = 11.84 GFMFGEE323 pKa = 4.63 DD324 pKa = 3.22 FASRR328 pKa = 11.84 YY329 pKa = 8.85 LSKK332 pKa = 10.78 AISTSDD338 pKa = 3.37 RR339 pKa = 11.84 KK340 pKa = 10.8 SKK342 pKa = 10.45 IAITIVSGISWNVEE356 pKa = 3.35 ILGCSPCVNVIDD368 pKa = 4.61 GSDD371 pKa = 3.38 YY372 pKa = 11.21 NIIRR376 pKa = 11.84 EE377 pKa = 3.95 ILEE380 pKa = 4.0 FPNRR384 pKa = 11.84 IIILGRR390 pKa = 11.84 KK391 pKa = 9.23 GSGKK395 pKa = 10.19 SRR397 pKa = 11.84 LSKK400 pKa = 10.22 MFAKK404 pKa = 10.51 LGYY407 pKa = 10.28 NIIDD411 pKa = 3.52 SDD413 pKa = 4.03 TYY415 pKa = 11.45 GRR417 pKa = 11.84 LLHH420 pKa = 6.42 LCKK423 pKa = 10.01 DD424 pKa = 3.92 TYY426 pKa = 11.12 TKK428 pKa = 11.39 DD429 pKa = 3.06 NFEE432 pKa = 4.72 RR433 pKa = 11.84 GLGYY437 pKa = 9.49 YY438 pKa = 10.31 LRR440 pKa = 11.84 LTKK443 pKa = 9.85 EE444 pKa = 3.96 EE445 pKa = 4.14 RR446 pKa = 11.84 DD447 pKa = 4.03 GIPSVFEE454 pKa = 4.16 LQMEE458 pKa = 4.35 ALLQKK463 pKa = 10.29 FKK465 pKa = 11.25 KK466 pKa = 9.95 RR467 pKa = 11.84 GLPSYY472 pKa = 10.96 CEE474 pKa = 4.4 LNDD477 pKa = 3.87 AYY479 pKa = 10.92 VGRR482 pKa = 11.84 LDD484 pKa = 4.2 RR485 pKa = 11.84 DD486 pKa = 3.31 LFLAYY491 pKa = 10.41 DD492 pKa = 3.57 KK493 pKa = 11.45 VYY495 pKa = 11.09 DD496 pKa = 3.73 KK497 pKa = 11.2 LIRR500 pKa = 11.84 TITPEE505 pKa = 3.92 DD506 pKa = 3.86 CRR508 pKa = 11.84 NMYY511 pKa = 10.17 LEE513 pKa = 4.96 KK514 pKa = 10.16 LWSSGGFDD522 pKa = 4.43 DD523 pKa = 6.67 LGFKK527 pKa = 10.57 PSLEE531 pKa = 3.94 KK532 pKa = 10.65 KK533 pKa = 10.37 AVIFCHH539 pKa = 5.69 TLAEE543 pKa = 4.31 QFQAMSAVLVEE554 pKa = 5.52 LIPAHH559 pKa = 5.06 STRR562 pKa = 11.84 TAVKK566 pKa = 9.99 IRR568 pKa = 11.84 GQGYY572 pKa = 8.56 SADD575 pKa = 3.9 SEE577 pKa = 5.45 LYY579 pKa = 9.53 LHH581 pKa = 7.53 DD582 pKa = 4.49 YY583 pKa = 10.29 YY584 pKa = 11.59 VSKK587 pKa = 10.88 NGNGAPKK594 pKa = 10.48 VGLGWLTHH602 pKa = 6.83 LLQSYY607 pKa = 9.45 VPEE610 pKa = 4.39 DD611 pKa = 3.13 WW612 pKa = 4.1
Molecular weight: 70.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.256
IPC2_protein 8.287
IPC_protein 8.141
Toseland 8.595
ProMoST 8.668
Dawson 9.033
Bjellqvist 8.975
Wikipedia 9.18
Rodwell 9.165
Grimsley 8.99
Solomon 9.092
Lehninger 9.048
Nozaki 9.033
DTASelect 8.858
Thurlkill 8.946
EMBOSS 9.165
Sillero 9.136
Patrickios 4.317
IPC_peptide 9.077
IPC2_peptide 7.658
IPC2.peptide.svr19 7.65
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13
0
13
9446
169
1433
726.6
82.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.965 ± 0.254
1.673 ± 0.14
5.897 ± 0.362
6.14 ± 0.392
4.552 ± 0.152
6.564 ± 0.301
2.149 ± 0.128
7.347 ± 0.347
5.685 ± 0.414
9.337 ± 0.254
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.721 ± 0.184
4.944 ± 0.337
4.266 ± 0.306
3.324 ± 0.318
5.77 ± 0.381
7.569 ± 0.494
5.166 ± 0.263
6.542 ± 0.246
1.461 ± 0.141
3.917 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here