Lachnospiraceae bacterium 3-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4984 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9IUF5|R9IUF5_9FIRM 23S rRNA (-C5)-methyltransferase OS=Lachnospiraceae bacterium 3-1 OX=397288 GN=C806_01682 PE=4 SV=1
MM1 pKa = 7.18KK2 pKa = 10.27RR3 pKa = 11.84KK4 pKa = 9.27IVSVLMASVMAAALLAGCGGNSNAPSNSDD33 pKa = 3.21SAGNEE38 pKa = 3.86EE39 pKa = 4.4KK40 pKa = 10.76SAGEE44 pKa = 4.01KK45 pKa = 10.28ADD47 pKa = 3.66SKK49 pKa = 11.28GGEE52 pKa = 4.34TVSGDD57 pKa = 3.43GQTLTIVYY65 pKa = 10.24NDD67 pKa = 3.86SNDD70 pKa = 3.57NGDD73 pKa = 3.73NSPVYY78 pKa = 10.04QWINQTYY85 pKa = 8.88EE86 pKa = 3.45NWEE89 pKa = 4.04KK90 pKa = 10.98KK91 pKa = 10.61DD92 pKa = 3.69EE93 pKa = 4.3VKK95 pKa = 10.94LDD97 pKa = 3.43IQALVATDD105 pKa = 3.26SDD107 pKa = 5.12YY108 pKa = 10.67VTKK111 pKa = 10.49IQTLVQDD118 pKa = 5.32DD119 pKa = 4.45STCPDD124 pKa = 4.15LFLEE128 pKa = 5.41DD129 pKa = 3.74TFQLNTDD136 pKa = 3.35VAAGYY141 pKa = 9.99VADD144 pKa = 4.39ITDD147 pKa = 4.51KK148 pKa = 10.6VAGWEE153 pKa = 4.13DD154 pKa = 3.52WNSAIIEE161 pKa = 4.17ALKK164 pKa = 10.51QATSGTDD171 pKa = 3.03GNTYY175 pKa = 10.56AVPISTDD182 pKa = 3.69VRR184 pKa = 11.84GLWYY188 pKa = 10.9NKK190 pKa = 10.27DD191 pKa = 3.32VFEE194 pKa = 4.38AAGLGRR200 pKa = 11.84EE201 pKa = 4.21WQPEE205 pKa = 3.91SWQDD209 pKa = 3.76ILDD212 pKa = 3.69ACAAIKK218 pKa = 9.06EE219 pKa = 4.26KK220 pKa = 11.29CEE222 pKa = 4.29DD223 pKa = 3.77DD224 pKa = 4.45VVPIWFACNSGEE236 pKa = 5.04AEE238 pKa = 4.06ATSMNTFQMLLAGTGDD254 pKa = 4.11SLCDD258 pKa = 3.84DD259 pKa = 3.48ATGVYY264 pKa = 9.9NLSSDD269 pKa = 3.78GVKK272 pKa = 10.66DD273 pKa = 3.28SLGFIKK279 pKa = 9.82TCLDD283 pKa = 3.16EE284 pKa = 6.2GYY286 pKa = 10.41LGTLSEE292 pKa = 4.7ILDD295 pKa = 4.1PSDD298 pKa = 3.13YY299 pKa = 10.72TYY301 pKa = 11.59ANQYY305 pKa = 9.41MSTAKK310 pKa = 10.6LGMYY314 pKa = 10.72LNGSWGYY321 pKa = 11.3NDD323 pKa = 4.04YY324 pKa = 11.0LQDD327 pKa = 3.52SGYY330 pKa = 10.04PMQGYY335 pKa = 7.85TDD337 pKa = 3.81EE338 pKa = 4.52TLSDD342 pKa = 3.71ALGFAQMPTQEE353 pKa = 4.01KK354 pKa = 9.61GGYY357 pKa = 7.25VTMSGGWSWAIANNSDD373 pKa = 3.69QKK375 pKa = 11.4DD376 pKa = 3.56LSFEE380 pKa = 4.65FITEE384 pKa = 4.13LMKK387 pKa = 10.48SDD389 pKa = 3.91NYY391 pKa = 8.78ITYY394 pKa = 9.97IEE396 pKa = 4.43GTGNLATRR404 pKa = 11.84NDD406 pKa = 3.63MMNFDD411 pKa = 4.85AYY413 pKa = 10.54SSRR416 pKa = 11.84MYY418 pKa = 10.32IDD420 pKa = 4.0EE421 pKa = 4.3ATAMSEE427 pKa = 3.94NAFFRR432 pKa = 11.84PHH434 pKa = 7.14DD435 pKa = 3.87EE436 pKa = 4.35NYY438 pKa = 10.91SKK440 pKa = 11.02VSSYY444 pKa = 10.75LYY446 pKa = 10.87EE447 pKa = 4.11MVDD450 pKa = 3.23TVIRR454 pKa = 11.84NNTDD458 pKa = 2.95TEE460 pKa = 4.41TALSDD465 pKa = 3.99FKK467 pKa = 11.16KK468 pKa = 10.78GAATAIGEE476 pKa = 4.21EE477 pKa = 4.67LIQQ480 pKa = 4.7

Molecular weight: 52.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9INK9|R9INK9_9FIRM Excisionase family DNA binding domain-containing protein OS=Lachnospiraceae bacterium 3-1 OX=397288 GN=C806_03001 PE=4 SV=1
MM1 pKa = 7.84AGMVLRR7 pKa = 11.84KK8 pKa = 8.44TGVVLLTILKK18 pKa = 9.45WILQAALVALKK29 pKa = 10.49LVLGIAKK36 pKa = 10.15LFLLLLSLVMRR47 pKa = 11.84IVLTMIGVSVGRR59 pKa = 11.84RR60 pKa = 3.23

Molecular weight: 6.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4984

0

4984

1447276

20

3497

290.4

32.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.08 ± 0.038

1.506 ± 0.012

5.28 ± 0.029

8.256 ± 0.041

4.125 ± 0.025

6.923 ± 0.036

1.784 ± 0.017

7.249 ± 0.034

7.303 ± 0.033

8.815 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.133 ± 0.018

4.475 ± 0.024

3.158 ± 0.024

3.725 ± 0.026

4.529 ± 0.028

5.849 ± 0.027

5.165 ± 0.029

6.471 ± 0.031

0.966 ± 0.013

4.207 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski