Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14) (Lettuce bottom rot fungus) (Rhizoctonia solani)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Cantharellales; Ceratobasidiaceae; Rhizoctonia; Rhizoctonia solani; Rhizoctonia solani AG-1

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12613 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M5C8Q5|M5C8Q5_THACB CVNH domain-containing protein OS=Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14) OX=1108050 GN=BN14_09895 PE=4 SV=1
MM1 pKa = 7.3AHH3 pKa = 7.4DD4 pKa = 4.45IVGQGDD10 pKa = 3.47WKK12 pKa = 9.98YY13 pKa = 8.11WYY15 pKa = 10.76AMRR18 pKa = 11.84FVDD21 pKa = 4.08RR22 pKa = 11.84DD23 pKa = 2.92IMIRR27 pKa = 11.84YY28 pKa = 8.1HH29 pKa = 6.86GEE31 pKa = 4.04GVGHH35 pKa = 6.22HH36 pKa = 6.03TGSMKK41 pKa = 10.66AHH43 pKa = 7.26ADD45 pKa = 3.24IQEE48 pKa = 4.35DD49 pKa = 4.27VPNRR53 pKa = 11.84TEE55 pKa = 4.08EE56 pKa = 4.14YY57 pKa = 9.85PNQQEE62 pKa = 4.2IPFDD66 pKa = 3.54EE67 pKa = 4.92HH68 pKa = 9.01SEE70 pKa = 4.19NNDD73 pKa = 3.44DD74 pKa = 5.25EE75 pKa = 5.32NDD77 pKa = 3.49EE78 pKa = 4.54DD79 pKa = 4.71VVTEE83 pKa = 3.95AALATEE89 pKa = 4.64LVGDD93 pKa = 4.08EE94 pKa = 4.92PSDD97 pKa = 3.58EE98 pKa = 4.96DD99 pKa = 3.58EE100 pKa = 4.92GFLSGEE106 pKa = 4.77DD107 pKa = 3.79YY108 pKa = 11.3DD109 pKa = 3.92SGYY112 pKa = 10.99YY113 pKa = 9.51EE114 pKa = 4.52SKK116 pKa = 11.05EE117 pKa = 3.88DD118 pKa = 3.85MEE120 pKa = 4.55PVQYY124 pKa = 10.74EE125 pKa = 4.24YY126 pKa = 11.69

Molecular weight: 14.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M5BND6|M5BND6_THACB ATG16 domain-containing protein OS=Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14) OX=1108050 GN=BN14_01788 PE=3 SV=1
MM1 pKa = 7.18FGSRR5 pKa = 11.84RR6 pKa = 11.84TRR8 pKa = 11.84ATHH11 pKa = 4.74TTHH14 pKa = 6.37TSTRR18 pKa = 11.84RR19 pKa = 11.84GGLGGMFRR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 9.89NPNRR33 pKa = 11.84RR34 pKa = 11.84AGGYY38 pKa = 8.99KK39 pKa = 10.13AALHH43 pKa = 5.99NPNTTSSGRR52 pKa = 11.84QHH54 pKa = 7.22AKK56 pKa = 9.97RR57 pKa = 11.84GLRR60 pKa = 11.84SMGRR64 pKa = 11.84GSEE67 pKa = 3.92AHH69 pKa = 6.13VPLSVRR75 pKa = 11.84IKK77 pKa = 10.45HH78 pKa = 5.83FLHH81 pKa = 6.18IGGRR85 pKa = 11.84RR86 pKa = 11.84HH87 pKa = 4.2TTTRR91 pKa = 11.84RR92 pKa = 11.84YY93 pKa = 9.93

Molecular weight: 10.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12613

0

12613

5771931

66

7643

457.6

50.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.554 ± 0.016

1.182 ± 0.008

5.415 ± 0.016

5.852 ± 0.021

3.478 ± 0.014

6.789 ± 0.022

2.492 ± 0.01

4.957 ± 0.017

4.62 ± 0.019

8.913 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.027 ± 0.008

3.631 ± 0.012

6.699 ± 0.027

3.813 ± 0.015

6.135 ± 0.023

8.717 ± 0.023

6.283 ± 0.017

6.248 ± 0.015

1.463 ± 0.008

2.699 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski