Mizuhopecten yessoensis (Japanese scallop) (Patinopecten yessoensis)
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22409 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A210PL05|A0A210PL05_MIZYE Pleckstrin homology domain-containing family G member 5 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT21310 PE=4 SV=1
MM1 pKa = 7.5 LNVCKK6 pKa = 10.36 IIPPFSRR13 pKa = 11.84 SVQSWVGDD21 pKa = 3.67 VICIGTLLIVRR32 pKa = 11.84 RR33 pKa = 11.84 QSRR36 pKa = 11.84 DD37 pKa = 2.55 GDD39 pKa = 4.12 VICIGILLIVRR50 pKa = 11.84 RR51 pKa = 11.84 QSWDD55 pKa = 2.99 GDD57 pKa = 4.13 VICIDD62 pKa = 4.12 GDD64 pKa = 4.02 IICIDD69 pKa = 3.81 GDD71 pKa = 4.44 VICLGILLIVRR82 pKa = 11.84 RR83 pKa = 11.84 QSWDD87 pKa = 3.24 GNVICIDD94 pKa = 3.76 GDD96 pKa = 4.1 VICIDD101 pKa = 4.12 GDD103 pKa = 4.15 VICIDD108 pKa = 3.61 VDD110 pKa = 4.17 VICIDD115 pKa = 4.15 GDD117 pKa = 3.89 INCIDD122 pKa = 4.32 GDD124 pKa = 4.4 VICIDD129 pKa = 3.83 GDD131 pKa = 3.99 IICILIFLIVRR142 pKa = 11.84 RR143 pKa = 11.84 QSMDD147 pKa = 2.86 GDD149 pKa = 4.34 VICIDD154 pKa = 4.21 GDD156 pKa = 3.98 VLCIDD161 pKa = 4.62 GDD163 pKa = 4.29 VICIDD168 pKa = 4.12 GDD170 pKa = 4.15 VICIDD175 pKa = 3.61 VDD177 pKa = 4.17 VICIDD182 pKa = 4.88 GDD184 pKa = 4.22 IIWIDD189 pKa = 3.43 GDD191 pKa = 4.44 VICLGILLIVRR202 pKa = 11.84 RR203 pKa = 11.84 QSWAGDD209 pKa = 3.69 VICIDD214 pKa = 4.1 GDD216 pKa = 4.0 IICIGIFLIVRR227 pKa = 11.84 RR228 pKa = 11.84 QSRR231 pKa = 11.84 DD232 pKa = 2.62 GDD234 pKa = 4.15 VICIDD239 pKa = 4.12 GDD241 pKa = 4.11 VICIDD246 pKa = 3.83 GDD248 pKa = 3.99 IICILIFLIVRR259 pKa = 11.84 RR260 pKa = 11.84 QSMDD264 pKa = 2.86 GDD266 pKa = 4.34 VICIDD271 pKa = 3.95 GDD273 pKa = 4.07 VICIRR278 pKa = 11.84 IFLIVRR284 pKa = 11.84 RR285 pKa = 11.84 QSWDD289 pKa = 2.8 GDD291 pKa = 3.82 VIYY294 pKa = 10.39 IGILLIVRR302 pKa = 11.84 RR303 pKa = 11.84 QSWDD307 pKa = 2.89 GDD309 pKa = 4.1 VICIRR314 pKa = 11.84 ILLIVRR320 pKa = 11.84 RR321 pKa = 11.84 QSWDD325 pKa = 2.86 GDD327 pKa = 4.11 VII329 pKa = 4.0
Molecular weight: 36.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.658
IPC2_protein 3.745
IPC_protein 3.834
Toseland 3.567
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.859
Rodwell 3.643
Grimsley 3.465
Solomon 3.859
Lehninger 3.808
Nozaki 3.961
DTASelect 4.355
Thurlkill 3.643
EMBOSS 3.859
Sillero 3.961
Patrickios 1.392
IPC_peptide 3.846
IPC2_peptide 3.923
IPC2.peptide.svr19 3.829
Protein with the highest isoelectric point:
>tr|A0A210PP45|A0A210PP45_MIZYE Multidrug resistance-associated protein 7 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT10635 PE=4 SV=1
MM1 pKa = 7.27 RR2 pKa = 11.84 TIGTMTMRR10 pKa = 11.84 TIGTMSVRR18 pKa = 11.84 TIGTMTMRR26 pKa = 11.84 TIGTMTMRR34 pKa = 11.84 TIGTMSVRR42 pKa = 11.84 TIGTMTMRR50 pKa = 11.84 TIGTMTMRR58 pKa = 11.84 TIGTMSVRR66 pKa = 11.84 TIGTMTMRR74 pKa = 11.84 TIGTMTMRR82 pKa = 11.84 TIGKK86 pKa = 8.59 MSVRR90 pKa = 11.84 TIGTMTLRR98 pKa = 11.84 KK99 pKa = 9.37 IGQQ102 pKa = 3.19
Molecular weight: 11.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.647
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.369
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.248
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
22409
0
22409
11322112
45
18501
505.2
56.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.426 ± 0.015
2.263 ± 0.021
5.907 ± 0.018
5.986 ± 0.024
3.702 ± 0.015
5.986 ± 0.034
2.611 ± 0.014
5.423 ± 0.018
6.105 ± 0.023
8.483 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.717 ± 0.011
4.828 ± 0.017
4.907 ± 0.022
4.266 ± 0.018
5.095 ± 0.021
8.364 ± 0.029
6.851 ± 0.029
6.683 ± 0.023
1.069 ± 0.008
3.325 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here