Streptomyces phage Ibantik

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UP82|A0A2U8UP82_9CAUD Uncharacterized protein OS=Streptomyces phage Ibantik OX=2182397 GN=1 PE=4 SV=1
MM1 pKa = 7.29KK2 pKa = 10.31HH3 pKa = 6.65RR4 pKa = 11.84IEE6 pKa = 4.18FTATLDD12 pKa = 3.73VYY14 pKa = 11.51DD15 pKa = 5.59DD16 pKa = 5.01DD17 pKa = 5.65EE18 pKa = 4.7EE19 pKa = 6.03LDD21 pKa = 4.29SISAAGWVHH30 pKa = 6.94AALLYY35 pKa = 10.45GDD37 pKa = 3.82KK38 pKa = 10.84HH39 pKa = 7.79SGEE42 pKa = 4.26FTSFLGEE49 pKa = 3.89VTDD52 pKa = 4.21YY53 pKa = 11.59YY54 pKa = 11.26SVEE57 pKa = 4.17DD58 pKa = 4.06EE59 pKa = 4.3EE60 pKa = 4.65

Molecular weight: 6.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8UNW4|A0A2U8UNW4_9CAUD Minor tail protein OS=Streptomyces phage Ibantik OX=2182397 GN=81 PE=4 SV=1
MM1 pKa = 7.54NARR4 pKa = 11.84IDD6 pKa = 3.33ASRR9 pKa = 11.84RR10 pKa = 11.84VASMPGKK17 pKa = 9.81QCPEE21 pKa = 4.02GPGGRR26 pKa = 11.84DD27 pKa = 4.18CYY29 pKa = 11.23CCGQPPGKK37 pKa = 9.8DD38 pKa = 2.24RR39 pKa = 11.84KK40 pKa = 9.05VKK42 pKa = 10.09RR43 pKa = 11.84RR44 pKa = 11.84AVKK47 pKa = 10.46RR48 pKa = 11.84GDD50 pKa = 3.25EE51 pKa = 4.29SKK53 pKa = 10.47FRR55 pKa = 11.84QSIRR59 pKa = 11.84KK60 pKa = 8.41YY61 pKa = 10.48LSRR64 pKa = 5.4

Molecular weight: 7.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

17028

32

1568

162.2

18.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.709 ± 0.282

1.398 ± 0.25

6.513 ± 0.202

7.464 ± 0.413

3.165 ± 0.173

8.416 ± 0.47

2.061 ± 0.168

4.17 ± 0.173

5.244 ± 0.318

7.629 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.437 ± 0.105

3.389 ± 0.222

4.763 ± 0.273

3.612 ± 0.283

6.378 ± 0.254

6.331 ± 0.224

5.749 ± 0.255

7.088 ± 0.259

2.114 ± 0.126

3.371 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski