Amino acid dipepetide frequency for Streptomyces phage Ibantik

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.986AlaAla: 8.986 ± 0.887
0.822AlaCys: 0.822 ± 0.24
4.816AlaAsp: 4.816 ± 0.525
6.813AlaGlu: 6.813 ± 0.656
2.525AlaPhe: 2.525 ± 0.317
7.694AlaGly: 7.694 ± 0.662
1.879AlaHis: 1.879 ± 0.306
3.935AlaIle: 3.935 ± 0.523
3.583AlaLys: 3.583 ± 0.548
7.165AlaLeu: 7.165 ± 0.562
2.408AlaMet: 2.408 ± 0.311
2.349AlaAsn: 2.349 ± 0.423
4.17AlaPro: 4.17 ± 0.647
3.583AlaGln: 3.583 ± 0.593
6.049AlaArg: 6.049 ± 0.552
5.11AlaSer: 5.11 ± 0.628
5.051AlaThr: 5.051 ± 0.603
6.343AlaVal: 6.343 ± 0.557
1.468AlaTrp: 1.468 ± 0.305
2.349AlaTyr: 2.349 ± 0.363
0.0AlaXaa: 0.0 ± 0.0
Cys
1.175CysAla: 1.175 ± 0.282
0.176CysCys: 0.176 ± 0.087
1.057CysAsp: 1.057 ± 0.281
0.763CysGlu: 0.763 ± 0.216
0.352CysPhe: 0.352 ± 0.155
1.468CysGly: 1.468 ± 0.393
0.529CysHis: 0.529 ± 0.154
0.47CysIle: 0.47 ± 0.166
0.587CysLys: 0.587 ± 0.172
0.822CysLeu: 0.822 ± 0.216
0.176CysMet: 0.176 ± 0.112
0.587CysAsn: 0.587 ± 0.201
0.705CysPro: 0.705 ± 0.228
0.763CysGln: 0.763 ± 0.24
0.881CysArg: 0.881 ± 0.274
1.057CysSer: 1.057 ± 0.274
0.587CysThr: 0.587 ± 0.17
0.705CysVal: 0.705 ± 0.204
0.587CysTrp: 0.587 ± 0.192
0.529CysTyr: 0.529 ± 0.152
0.0CysXaa: 0.0 ± 0.0
Asp
4.64AspAla: 4.64 ± 0.481
1.41AspCys: 1.41 ± 0.372
4.052AspAsp: 4.052 ± 0.575
5.579AspGlu: 5.579 ± 0.739
1.997AspPhe: 1.997 ± 0.256
6.225AspGly: 6.225 ± 0.775
1.116AspHis: 1.116 ± 0.281
2.878AspIle: 2.878 ± 0.423
2.76AspLys: 2.76 ± 0.437
6.754AspLeu: 6.754 ± 0.625
1.821AspMet: 1.821 ± 0.317
1.938AspAsn: 1.938 ± 0.33
3.7AspPro: 3.7 ± 0.444
2.056AspGln: 2.056 ± 0.414
3.817AspArg: 3.817 ± 0.532
3.7AspSer: 3.7 ± 0.446
2.995AspThr: 2.995 ± 0.385
4.17AspVal: 4.17 ± 0.486
1.586AspTrp: 1.586 ± 0.352
1.938AspTyr: 1.938 ± 0.367
0.0AspXaa: 0.0 ± 0.0
Glu
8.575GluAla: 8.575 ± 0.75
0.705GluCys: 0.705 ± 0.258
6.225GluAsp: 6.225 ± 0.687
5.756GluGlu: 5.756 ± 0.777
2.525GluPhe: 2.525 ± 0.4
5.051GluGly: 5.051 ± 0.613
1.351GluHis: 1.351 ± 0.315
3.348GluIle: 3.348 ± 0.4
3.054GluLys: 3.054 ± 0.457
6.225GluLeu: 6.225 ± 0.613
2.467GluMet: 2.467 ± 0.421
2.29GluAsn: 2.29 ± 0.35
2.702GluPro: 2.702 ± 0.36
2.114GluGln: 2.114 ± 0.417
4.581GluArg: 4.581 ± 0.534
3.583GluSer: 3.583 ± 0.494
2.937GluThr: 2.937 ± 0.479
6.402GluVal: 6.402 ± 0.85
1.703GluTrp: 1.703 ± 0.301
2.995GluTyr: 2.995 ± 0.479
0.0GluXaa: 0.0 ± 0.0
Phe
2.349PheAla: 2.349 ± 0.341
0.411PheCys: 0.411 ± 0.178
1.879PheAsp: 1.879 ± 0.342
2.643PheGlu: 2.643 ± 0.433
0.822PhePhe: 0.822 ± 0.278
2.878PheGly: 2.878 ± 0.395
0.998PheHis: 0.998 ± 0.26
1.292PheIle: 1.292 ± 0.35
1.351PheLys: 1.351 ± 0.258
2.114PheLeu: 2.114 ± 0.416
0.822PheMet: 0.822 ± 0.185
0.998PheAsn: 0.998 ± 0.295
1.41PhePro: 1.41 ± 0.239
1.175PheGln: 1.175 ± 0.285
1.703PheArg: 1.703 ± 0.293
2.232PheSer: 2.232 ± 0.374
2.29PheThr: 2.29 ± 0.372
2.408PheVal: 2.408 ± 0.359
0.763PheTrp: 0.763 ± 0.225
1.116PheTyr: 1.116 ± 0.257
0.0PheXaa: 0.0 ± 0.0
Gly
4.992GlyAla: 4.992 ± 0.736
0.998GlyCys: 0.998 ± 0.258
5.99GlyAsp: 5.99 ± 0.581
5.579GlyGlu: 5.579 ± 0.499
2.643GlyPhe: 2.643 ± 0.432
7.635GlyGly: 7.635 ± 0.959
1.586GlyHis: 1.586 ± 0.282
2.76GlyIle: 2.76 ± 0.581
5.814GlyLys: 5.814 ± 0.754
6.225GlyLeu: 6.225 ± 0.82
1.997GlyMet: 1.997 ± 0.32
3.465GlyAsn: 3.465 ± 0.67
3.876GlyPro: 3.876 ± 0.461
2.995GlyGln: 2.995 ± 0.409
4.933GlyArg: 4.933 ± 0.455
6.108GlySer: 6.108 ± 0.637
4.522GlyThr: 4.522 ± 0.478
6.049GlyVal: 6.049 ± 0.668
1.997GlyTrp: 1.997 ± 0.402
3.994GlyTyr: 3.994 ± 0.422
0.0GlyXaa: 0.0 ± 0.0
His
1.351HisAla: 1.351 ± 0.265
0.47HisCys: 0.47 ± 0.167
0.94HisAsp: 0.94 ± 0.227
1.175HisGlu: 1.175 ± 0.297
1.057HisPhe: 1.057 ± 0.222
1.821HisGly: 1.821 ± 0.381
0.529HisHis: 0.529 ± 0.17
0.998HisIle: 0.998 ± 0.266
0.881HisLys: 0.881 ± 0.265
2.114HisLeu: 2.114 ± 0.336
0.352HisMet: 0.352 ± 0.115
0.47HisAsn: 0.47 ± 0.145
1.468HisPro: 1.468 ± 0.265
0.822HisGln: 0.822 ± 0.189
1.41HisArg: 1.41 ± 0.294
1.116HisSer: 1.116 ± 0.263
1.351HisThr: 1.351 ± 0.268
1.292HisVal: 1.292 ± 0.279
0.646HisTrp: 0.646 ± 0.184
0.352HisTyr: 0.352 ± 0.132
0.0HisXaa: 0.0 ± 0.0
Ile
2.76IleAla: 2.76 ± 0.388
0.47IleCys: 0.47 ± 0.196
3.289IleAsp: 3.289 ± 0.434
2.995IleGlu: 2.995 ± 0.526
0.94IlePhe: 0.94 ± 0.235
2.584IleGly: 2.584 ± 0.414
1.057IleHis: 1.057 ± 0.314
1.938IleIle: 1.938 ± 0.284
1.938IleLys: 1.938 ± 0.333
2.76IleLeu: 2.76 ± 0.432
1.057IleMet: 1.057 ± 0.24
1.41IleAsn: 1.41 ± 0.353
2.937IlePro: 2.937 ± 0.493
1.644IleGln: 1.644 ± 0.362
4.346IleArg: 4.346 ± 0.474
2.467IleSer: 2.467 ± 0.356
2.995IleThr: 2.995 ± 0.455
1.879IleVal: 1.879 ± 0.303
1.057IleTrp: 1.057 ± 0.278
1.175IleTyr: 1.175 ± 0.218
0.0IleXaa: 0.0 ± 0.0
Lys
5.756LysAla: 5.756 ± 0.596
0.705LysCys: 0.705 ± 0.287
3.406LysAsp: 3.406 ± 0.473
3.465LysGlu: 3.465 ± 0.512
1.821LysPhe: 1.821 ± 0.394
3.583LysGly: 3.583 ± 0.5
1.351LysHis: 1.351 ± 0.315
1.821LysIle: 1.821 ± 0.303
3.23LysLys: 3.23 ± 0.748
3.935LysLeu: 3.935 ± 0.525
1.644LysMet: 1.644 ± 0.295
1.879LysAsn: 1.879 ± 0.328
2.29LysPro: 2.29 ± 0.413
1.997LysGln: 1.997 ± 0.367
2.76LysArg: 2.76 ± 0.488
3.817LysSer: 3.817 ± 0.521
3.759LysThr: 3.759 ± 0.499
3.113LysVal: 3.113 ± 0.396
0.881LysTrp: 0.881 ± 0.217
1.233LysTyr: 1.233 ± 0.29
0.0LysXaa: 0.0 ± 0.0
Leu
6.108LeuAla: 6.108 ± 0.501
1.233LeuCys: 1.233 ± 0.302
5.403LeuAsp: 5.403 ± 0.636
6.225LeuGlu: 6.225 ± 0.663
2.114LeuPhe: 2.114 ± 0.312
6.108LeuGly: 6.108 ± 0.573
1.468LeuHis: 1.468 ± 0.259
3.289LeuIle: 3.289 ± 0.567
4.17LeuLys: 4.17 ± 0.629
5.403LeuLeu: 5.403 ± 0.525
2.173LeuMet: 2.173 ± 0.324
2.467LeuAsn: 2.467 ± 0.387
3.817LeuPro: 3.817 ± 0.41
1.938LeuGln: 1.938 ± 0.351
4.757LeuArg: 4.757 ± 0.482
5.638LeuSer: 5.638 ± 0.525
4.581LeuThr: 4.581 ± 0.534
5.873LeuVal: 5.873 ± 0.539
1.292LeuTrp: 1.292 ± 0.352
2.232LeuTyr: 2.232 ± 0.373
0.0LeuXaa: 0.0 ± 0.0
Met
2.408MetAla: 2.408 ± 0.355
0.294MetCys: 0.294 ± 0.133
1.057MetAsp: 1.057 ± 0.218
1.292MetGlu: 1.292 ± 0.295
0.881MetPhe: 0.881 ± 0.195
1.351MetGly: 1.351 ± 0.323
0.235MetHis: 0.235 ± 0.112
0.763MetIle: 0.763 ± 0.21
1.586MetLys: 1.586 ± 0.284
1.351MetLeu: 1.351 ± 0.285
0.294MetMet: 0.294 ± 0.116
1.468MetAsn: 1.468 ± 0.301
1.116MetPro: 1.116 ± 0.283
0.587MetGln: 0.587 ± 0.198
1.703MetArg: 1.703 ± 0.279
2.408MetSer: 2.408 ± 0.319
1.997MetThr: 1.997 ± 0.339
2.467MetVal: 2.467 ± 0.437
0.411MetTrp: 0.411 ± 0.169
0.705MetTyr: 0.705 ± 0.198
0.0MetXaa: 0.0 ± 0.0
Asn
3.23AsnAla: 3.23 ± 0.422
0.646AsnCys: 0.646 ± 0.2
1.527AsnAsp: 1.527 ± 0.314
2.702AsnGlu: 2.702 ± 0.384
0.822AsnPhe: 0.822 ± 0.231
3.759AsnGly: 3.759 ± 0.396
0.47AsnHis: 0.47 ± 0.159
1.351AsnIle: 1.351 ± 0.264
1.644AsnLys: 1.644 ± 0.325
2.76AsnLeu: 2.76 ± 0.36
0.47AsnMet: 0.47 ± 0.166
1.057AsnAsn: 1.057 ± 0.31
2.173AsnPro: 2.173 ± 0.378
1.821AsnGln: 1.821 ± 0.507
2.525AsnArg: 2.525 ± 0.358
1.644AsnSer: 1.644 ± 0.327
1.762AsnThr: 1.762 ± 0.418
1.762AsnVal: 1.762 ± 0.339
0.646AsnTrp: 0.646 ± 0.186
1.116AsnTyr: 1.116 ± 0.259
0.0AsnXaa: 0.0 ± 0.0
Pro
3.935ProAla: 3.935 ± 0.552
0.881ProCys: 0.881 ± 0.236
3.583ProAsp: 3.583 ± 0.501
4.346ProGlu: 4.346 ± 0.564
1.527ProPhe: 1.527 ± 0.275
4.346ProGly: 4.346 ± 0.675
0.998ProHis: 0.998 ± 0.263
1.644ProIle: 1.644 ± 0.279
2.643ProLys: 2.643 ± 0.298
2.702ProLeu: 2.702 ± 0.311
1.175ProMet: 1.175 ± 0.233
1.879ProAsn: 1.879 ± 0.345
1.175ProPro: 1.175 ± 0.317
1.997ProGln: 1.997 ± 0.381
2.525ProArg: 2.525 ± 0.475
3.113ProSer: 3.113 ± 0.55
2.76ProThr: 2.76 ± 0.411
3.935ProVal: 3.935 ± 0.446
0.94ProTrp: 0.94 ± 0.269
1.527ProTyr: 1.527 ± 0.312
0.0ProXaa: 0.0 ± 0.0
Gln
3.524GlnAla: 3.524 ± 0.475
0.294GlnCys: 0.294 ± 0.147
1.703GlnAsp: 1.703 ± 0.295
2.937GlnGlu: 2.937 ± 0.496
1.351GlnPhe: 1.351 ± 0.274
2.819GlnGly: 2.819 ± 0.37
0.881GlnHis: 0.881 ± 0.253
1.703GlnIle: 1.703 ± 0.342
1.997GlnLys: 1.997 ± 0.409
3.524GlnLeu: 3.524 ± 0.539
0.94GlnMet: 0.94 ± 0.404
1.351GlnAsn: 1.351 ± 0.344
1.644GlnPro: 1.644 ± 0.397
1.057GlnGln: 1.057 ± 0.293
2.29GlnArg: 2.29 ± 0.467
1.762GlnSer: 1.762 ± 0.336
1.586GlnThr: 1.586 ± 0.41
2.525GlnVal: 2.525 ± 0.523
0.705GlnTrp: 0.705 ± 0.183
1.527GlnTyr: 1.527 ± 0.306
0.0GlnXaa: 0.0 ± 0.0
Arg
5.403ArgAla: 5.403 ± 0.474
1.175ArgCys: 1.175 ± 0.271
4.405ArgAsp: 4.405 ± 0.551
4.581ArgGlu: 4.581 ± 0.492
2.76ArgPhe: 2.76 ± 0.37
4.522ArgGly: 4.522 ± 0.546
1.527ArgHis: 1.527 ± 0.353
3.465ArgIle: 3.465 ± 0.424
3.348ArgLys: 3.348 ± 0.47
4.698ArgLeu: 4.698 ± 0.451
1.41ArgMet: 1.41 ± 0.265
2.056ArgAsn: 2.056 ± 0.291
2.525ArgPro: 2.525 ± 0.4
2.467ArgGln: 2.467 ± 0.647
4.17ArgArg: 4.17 ± 0.53
3.171ArgSer: 3.171 ± 0.43
3.113ArgThr: 3.113 ± 0.352
4.992ArgVal: 4.992 ± 0.616
1.644ArgTrp: 1.644 ± 0.307
2.349ArgTyr: 2.349 ± 0.389
0.0ArgXaa: 0.0 ± 0.0
Ser
6.284SerAla: 6.284 ± 0.519
0.881SerCys: 0.881 ± 0.258
3.524SerAsp: 3.524 ± 0.488
2.819SerGlu: 2.819 ± 0.451
2.29SerPhe: 2.29 ± 0.343
6.695SerGly: 6.695 ± 0.894
1.41SerHis: 1.41 ± 0.375
2.643SerIle: 2.643 ± 0.353
3.935SerLys: 3.935 ± 0.353
4.64SerLeu: 4.64 ± 0.607
1.41SerMet: 1.41 ± 0.279
1.879SerAsn: 1.879 ± 0.455
3.289SerPro: 3.289 ± 0.411
2.702SerGln: 2.702 ± 0.502
3.935SerArg: 3.935 ± 0.497
4.463SerSer: 4.463 ± 0.546
3.171SerThr: 3.171 ± 0.343
4.405SerVal: 4.405 ± 0.544
1.175SerTrp: 1.175 ± 0.228
1.762SerTyr: 1.762 ± 0.324
0.0SerXaa: 0.0 ± 0.0
Thr
5.227ThrAla: 5.227 ± 0.607
0.763ThrCys: 0.763 ± 0.232
3.524ThrAsp: 3.524 ± 0.456
4.581ThrGlu: 4.581 ± 0.558
1.879ThrPhe: 1.879 ± 0.316
5.638ThrGly: 5.638 ± 0.719
1.292ThrHis: 1.292 ± 0.246
2.173ThrIle: 2.173 ± 0.365
3.113ThrLys: 3.113 ± 0.356
4.522ThrLeu: 4.522 ± 0.593
0.646ThrMet: 0.646 ± 0.182
1.997ThrAsn: 1.997 ± 0.328
3.759ThrPro: 3.759 ± 0.475
1.351ThrGln: 1.351 ± 0.318
3.465ThrArg: 3.465 ± 0.46
3.113ThrSer: 3.113 ± 0.64
3.113ThrThr: 3.113 ± 0.426
4.052ThrVal: 4.052 ± 0.659
1.233ThrTrp: 1.233 ± 0.239
2.056ThrTyr: 2.056 ± 0.3
0.0ThrXaa: 0.0 ± 0.0
Val
5.227ValAla: 5.227 ± 0.468
0.763ValCys: 0.763 ± 0.21
5.051ValAsp: 5.051 ± 0.696
5.403ValGlu: 5.403 ± 0.606
1.41ValPhe: 1.41 ± 0.24
5.227ValGly: 5.227 ± 0.724
1.116ValHis: 1.116 ± 0.258
3.524ValIle: 3.524 ± 0.444
3.7ValLys: 3.7 ± 0.488
4.992ValLeu: 4.992 ± 0.724
1.821ValMet: 1.821 ± 0.251
2.349ValAsn: 2.349 ± 0.426
2.878ValPro: 2.878 ± 0.383
2.878ValGln: 2.878 ± 0.424
4.17ValArg: 4.17 ± 0.706
5.344ValSer: 5.344 ± 0.687
5.873ValThr: 5.873 ± 0.546
4.875ValVal: 4.875 ± 0.599
1.233ValTrp: 1.233 ± 0.234
3.054ValTyr: 3.054 ± 0.476
0.0ValXaa: 0.0 ± 0.0
Trp
2.584TrpAla: 2.584 ± 0.508
0.235TrpCys: 0.235 ± 0.115
1.997TrpAsp: 1.997 ± 0.38
1.997TrpGlu: 1.997 ± 0.393
0.646TrpPhe: 0.646 ± 0.212
1.351TrpGly: 1.351 ± 0.33
0.529TrpHis: 0.529 ± 0.169
0.352TrpIle: 0.352 ± 0.143
1.351TrpLys: 1.351 ± 0.261
1.292TrpLeu: 1.292 ± 0.294
0.47TrpMet: 0.47 ± 0.206
0.646TrpAsn: 0.646 ± 0.191
0.998TrpPro: 0.998 ± 0.313
0.529TrpGln: 0.529 ± 0.17
1.351TrpArg: 1.351 ± 0.265
1.233TrpSer: 1.233 ± 0.291
1.292TrpThr: 1.292 ± 0.233
1.233TrpVal: 1.233 ± 0.274
0.176TrpTrp: 0.176 ± 0.09
0.881TrpTyr: 0.881 ± 0.264
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.584TyrAla: 2.584 ± 0.352
0.646TyrCys: 0.646 ± 0.203
1.703TyrAsp: 1.703 ± 0.286
2.995TyrGlu: 2.995 ± 0.416
1.292TyrPhe: 1.292 ± 0.3
3.406TyrGly: 3.406 ± 0.442
0.294TyrHis: 0.294 ± 0.119
1.351TyrIle: 1.351 ± 0.275
1.762TyrLys: 1.762 ± 0.294
2.29TyrLeu: 2.29 ± 0.363
0.763TyrMet: 0.763 ± 0.256
1.351TyrAsn: 1.351 ± 0.236
0.998TyrPro: 0.998 ± 0.218
1.644TyrGln: 1.644 ± 0.295
2.408TyrArg: 2.408 ± 0.333
2.232TyrSer: 2.232 ± 0.425
1.762TyrThr: 1.762 ± 0.318
2.408TyrVal: 2.408 ± 0.384
0.998TyrTrp: 0.998 ± 0.209
0.822TyrTyr: 0.822 ± 0.251
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 105 proteins (17028 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski