Dictyobacter kobayashii
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8237 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A402APW9|A0A402APW9_9CHLR Uncharacterized protein OS=Dictyobacter kobayashii OX=2014872 GN=KDK_49670 PE=3 SV=1
MM1 pKa = 7.85 ADD3 pKa = 3.75 LAAVAYY9 pKa = 7.22 EE10 pKa = 4.36 AYY12 pKa = 10.68 VEE14 pKa = 4.51 GINPIGWGDD23 pKa = 3.63 FGEE26 pKa = 4.29 DD27 pKa = 3.03 HH28 pKa = 6.68 VGLVGVDD35 pKa = 3.7 LGADD39 pKa = 3.59 EE40 pKa = 4.67 SQAFAGTPDD49 pKa = 3.99 MGVDD53 pKa = 3.08 WHH55 pKa = 7.16 DD56 pKa = 3.44 VASKK60 pKa = 11.03 VEE62 pKa = 3.91 EE63 pKa = 4.17 HH64 pKa = 7.15 DD65 pKa = 3.61 AGGGFGAYY73 pKa = 9.65 SGQLLL78 pKa = 3.72
Molecular weight: 8.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.694
IPC_protein 3.63
Toseland 3.427
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.605
Rodwell 3.465
Grimsley 3.35
Solomon 3.605
Lehninger 3.567
Nozaki 3.77
DTASelect 3.973
Thurlkill 3.503
EMBOSS 3.605
Sillero 3.757
Patrickios 0.693
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.722
Protein with the highest isoelectric point:
>tr|A0A402AJI0|A0A402AJI0_9CHLR Phosphate-binding protein OS=Dictyobacter kobayashii OX=2014872 GN=KDK_30240 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.45 RR3 pKa = 11.84 TWQPKK8 pKa = 8.26 RR9 pKa = 11.84 IPRR12 pKa = 11.84 KK13 pKa = 9.21 RR14 pKa = 11.84 EE15 pKa = 3.41 HH16 pKa = 7.15 GFMKK20 pKa = 10.47 RR21 pKa = 11.84 MSTRR25 pKa = 11.84 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLKK32 pKa = 10.37 SRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.48 GRR39 pKa = 11.84 WSLTVV44 pKa = 3.02
Molecular weight: 5.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.425
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.369
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.087
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8237
0
8237
2351450
39
2887
285.5
31.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.351 ± 0.031
0.989 ± 0.009
4.768 ± 0.021
5.679 ± 0.036
3.657 ± 0.021
6.988 ± 0.028
2.581 ± 0.015
6.089 ± 0.023
3.371 ± 0.02
10.976 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.329 ± 0.011
3.496 ± 0.025
5.086 ± 0.019
5.291 ± 0.025
5.835 ± 0.027
6.17 ± 0.022
5.957 ± 0.025
6.686 ± 0.025
1.518 ± 0.014
3.184 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here