Pectinatus cerevisiiphilus
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2417 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R3K6B5|A0A4R3K6B5_9FIRM Citrate lyase subunit beta/citryl-CoA lyase OS=Pectinatus cerevisiiphilus OX=86956 GN=EDC37_11187 PE=4 SV=1
MM1 pKa = 7.37 KK2 pKa = 10.21 KK3 pKa = 9.1 WVCSVCGWIYY13 pKa = 10.98 DD14 pKa = 4.06 EE15 pKa = 5.06 EE16 pKa = 5.89 KK17 pKa = 11.03 GDD19 pKa = 4.17 PDD21 pKa = 3.73 YY22 pKa = 11.76 DD23 pKa = 3.94 LAPGVPFADD32 pKa = 3.98 LPDD35 pKa = 5.13 DD36 pKa = 4.64 FVCPVCGVGKK46 pKa = 9.92 DD47 pKa = 3.51 QFEE50 pKa = 4.44 EE51 pKa = 4.37 EE52 pKa = 4.19 QQ53 pKa = 3.34
Molecular weight: 5.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.795
IPC_protein 3.719
Toseland 3.516
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.427
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.592
EMBOSS 3.694
Sillero 3.846
Patrickios 0.693
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.752
Protein with the highest isoelectric point:
>tr|A0A4R3K9B4|A0A4R3K9B4_9FIRM Biotin synthase OS=Pectinatus cerevisiiphilus OX=86956 GN=EDC37_10629 PE=4 SV=1
MM1 pKa = 7.62 NIIQVLEE8 pKa = 3.9 QEE10 pKa = 4.4 QLKK13 pKa = 10.65 KK14 pKa = 10.57 DD15 pKa = 3.36 IPQFQPGDD23 pKa = 3.7 TVRR26 pKa = 11.84 VHH28 pKa = 5.62 VKK30 pKa = 9.92 VVEE33 pKa = 4.45 GTHH36 pKa = 5.88 EE37 pKa = 4.94 RR38 pKa = 11.84 IQMFEE43 pKa = 3.91 GVVIARR49 pKa = 11.84 QGSGVRR55 pKa = 11.84 EE56 pKa = 4.13 MFTVRR61 pKa = 11.84 RR62 pKa = 11.84 VSYY65 pKa = 10.36 GIGVEE70 pKa = 4.04 RR71 pKa = 11.84 TFPVHH76 pKa = 6.77 SPRR79 pKa = 11.84 LEE81 pKa = 4.44 KK82 pKa = 10.08 IDD84 pKa = 4.12 VIRR87 pKa = 11.84 KK88 pKa = 8.33 GVVRR92 pKa = 11.84 RR93 pKa = 11.84 AKK95 pKa = 10.35 LYY97 pKa = 9.51 YY98 pKa = 10.2 LRR100 pKa = 11.84 NLTGKK105 pKa = 9.91 AARR108 pKa = 11.84 IKK110 pKa = 10.23 EE111 pKa = 4.07 RR112 pKa = 11.84 RR113 pKa = 3.58
Molecular weight: 13.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.322
IPC2_protein 9.765
IPC_protein 10.687
Toseland 10.921
ProMoST 10.994
Dawson 10.979
Bjellqvist 10.716
Wikipedia 11.228
Rodwell 11.155
Grimsley 11.023
Solomon 11.169
Lehninger 11.125
Nozaki 10.891
DTASelect 10.716
Thurlkill 10.906
EMBOSS 11.33
Sillero 10.921
Patrickios 10.906
IPC_peptide 11.184
IPC2_peptide 9.56
IPC2.peptide.svr19 8.772
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2417
0
2417
764205
29
2221
316.2
35.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.42 ± 0.053
1.276 ± 0.022
5.467 ± 0.032
5.837 ± 0.045
4.159 ± 0.036
6.805 ± 0.045
1.961 ± 0.022
8.409 ± 0.047
7.365 ± 0.048
9.293 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.915 ± 0.024
4.929 ± 0.04
3.576 ± 0.03
3.376 ± 0.033
3.924 ± 0.031
5.698 ± 0.04
5.49 ± 0.032
6.607 ± 0.045
0.838 ± 0.018
3.654 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here