Streptococcus satellite phage Javan319
Average proteome isoelectric point is 7.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZJX3|A0A4D5ZJX3_9VIRU Integrase OS=Streptococcus satellite phage Javan319 OX=2558633 GN=JavanS319_0001 PE=3 SV=1
MM1 pKa = 6.88 TLKK4 pKa = 10.02 TFSDD8 pKa = 3.76 EE9 pKa = 4.34 AKK11 pKa = 9.6 TFTFTYY17 pKa = 9.85 EE18 pKa = 4.03 FQDD21 pKa = 3.51 LDD23 pKa = 3.6 TAMVAGHH30 pKa = 7.15 ALLGYY35 pKa = 7.54 MTGTYY40 pKa = 8.23 CQPVISITYY49 pKa = 8.38 KK50 pKa = 11.0 DD51 pKa = 3.63 KK52 pKa = 9.79 GTLVAEE58 pKa = 4.26 YY59 pKa = 11.19 VEE61 pKa = 4.12 DD62 pKa = 3.62 HH63 pKa = 6.67 KK64 pKa = 11.65 LNKK67 pKa = 8.14 TFKK70 pKa = 10.29 RR71 pKa = 11.84 ICDD74 pKa = 3.7 SFKK77 pKa = 10.87 DD78 pKa = 4.26 YY79 pKa = 11.51 YY80 pKa = 9.82 NQPVDD85 pKa = 4.45 DD86 pKa = 4.12 EE87 pKa = 4.7 AFEE90 pKa = 4.14 EE91 pKa = 5.01 RR92 pKa = 11.84 YY93 pKa = 9.61 KK94 pKa = 10.15 QARR97 pKa = 11.84 VLQLKK102 pKa = 9.69 EE103 pKa = 4.27 SEE105 pKa = 4.49 DD106 pKa = 4.18 FEE108 pKa = 5.17 SLLNKK113 pKa = 7.83 VTGYY117 pKa = 10.23 EE118 pKa = 4.69 LEE120 pKa = 4.29 LLDD123 pKa = 4.15 YY124 pKa = 11.08 ADD126 pKa = 5.31 RR127 pKa = 11.84 LLSDD131 pKa = 4.21 KK132 pKa = 9.93 PIPMDD137 pKa = 3.3 SMTAFGTLEE146 pKa = 3.9 MLGDD150 pKa = 3.83 EE151 pKa = 5.38 SISLLQKK158 pKa = 10.52 LDD160 pKa = 3.26 VEE162 pKa = 4.69 GEE164 pKa = 4.0 YY165 pKa = 10.4 RR166 pKa = 11.84 GLVGYY171 pKa = 10.43 SDD173 pKa = 3.51 QQ174 pKa = 4.33
Molecular weight: 20.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.348
IPC2_protein 4.393
IPC_protein 4.329
Toseland 4.151
ProMoST 4.457
Dawson 4.291
Bjellqvist 4.444
Wikipedia 4.177
Rodwell 4.164
Grimsley 4.062
Solomon 4.291
Lehninger 4.24
Nozaki 4.406
DTASelect 4.584
Thurlkill 4.177
EMBOSS 4.202
Sillero 4.444
Patrickios 3.439
IPC_peptide 4.291
IPC2_peptide 4.431
IPC2.peptide.svr19 4.368
Protein with the highest isoelectric point:
>tr|A0A4D5ZPN1|A0A4D5ZPN1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan319 OX=2558633 GN=JavanS319_0009 PE=4 SV=1
MM1 pKa = 7.45 EE2 pKa = 6.05 PGWSPKK8 pKa = 9.9 RR9 pKa = 11.84 GGYY12 pKa = 8.86 MFDD15 pKa = 4.06 SFPEE19 pKa = 3.91 RR20 pKa = 11.84 PKK22 pKa = 10.83 YY23 pKa = 9.68 MKK25 pKa = 9.67 QKK27 pKa = 10.27 RR28 pKa = 11.84 YY29 pKa = 8.88 YY30 pKa = 9.43 KK31 pKa = 9.91 HH32 pKa = 4.88 YY33 pKa = 10.68 QKK35 pKa = 10.61 FVYY38 pKa = 8.34 YY39 pKa = 8.95 TRR41 pKa = 11.84 KK42 pKa = 10.11 GDD44 pKa = 3.92 RR45 pKa = 11.84 FWLNGLSNLKK55 pKa = 10.28
Molecular weight: 6.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.218
IPC2_protein 9.838
IPC_protein 9.999
Toseland 10.131
ProMoST 9.94
Dawson 10.365
Bjellqvist 10.058
Wikipedia 10.555
Rodwell 10.789
Grimsley 10.452
Solomon 10.394
Lehninger 10.35
Nozaki 10.087
DTASelect 10.058
Thurlkill 10.189
EMBOSS 10.54
Sillero 10.262
Patrickios 10.423
IPC_peptide 10.379
IPC2_peptide 8.726
IPC2.peptide.svr19 8.694
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14
0
14
2334
51
409
166.7
19.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.613 ± 0.279
0.428 ± 0.125
5.398 ± 0.558
8.183 ± 0.64
3.685 ± 0.382
4.584 ± 0.332
1.714 ± 0.23
6.084 ± 0.593
9.94 ± 0.661
11.525 ± 0.451
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.356 ± 0.399
5.613 ± 0.548
2.442 ± 0.415
5.184 ± 0.362
4.841 ± 0.369
5.913 ± 0.354
6.084 ± 0.575
5.27 ± 0.309
0.771 ± 0.199
4.37 ± 0.528
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here