Arthrobacter nitrophenolicus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3633 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L8TT60|L8TT60_9MICC DUF948 domain-containing protein OS=Arthrobacter nitrophenolicus OX=683150 GN=E2R57_05835 PE=4 SV=1
MM1 pKa = 6.85LTAYY5 pKa = 10.23YY6 pKa = 10.84VLLDD10 pKa = 4.5DD11 pKa = 5.31GGSSGVRR18 pKa = 11.84FGCNDD23 pKa = 3.41SLVGLARR30 pKa = 11.84AAPGTEE36 pKa = 4.04PLLTAAMNALLQGAGDD52 pKa = 4.17ADD54 pKa = 3.78IPLDD58 pKa = 4.57PIEE61 pKa = 4.67VSDD64 pKa = 4.98VYY66 pKa = 11.26NALDD70 pKa = 3.81GSEE73 pKa = 4.22LTFLSGSFDD82 pKa = 3.37GTTVTVYY89 pKa = 10.84LAGTLSLGGACDD101 pKa = 3.67VPRR104 pKa = 11.84VEE106 pKa = 5.22AQLTHH111 pKa = 5.84TAVAAVGAIRR121 pKa = 11.84ADD123 pKa = 3.7VYY125 pKa = 11.87VNGQPLSEE133 pKa = 4.58VLRR136 pKa = 11.84QDD138 pKa = 2.98

Molecular weight: 14.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L8TU56|L8TU56_9MICC Extracellular solute-binding protein family 1 protein OS=Arthrobacter nitrophenolicus OX=683150 GN=G205_09558 PE=4 SV=1
MM1 pKa = 7.64SSDD4 pKa = 4.34ALVADD9 pKa = 4.2QTDD12 pKa = 3.75HH13 pKa = 6.84PGEE16 pKa = 4.26PVAVTAAGALPWRR29 pKa = 11.84VTNDD33 pKa = 3.19RR34 pKa = 11.84LEE36 pKa = 3.92VLLIHH41 pKa = 7.32RR42 pKa = 11.84PRR44 pKa = 11.84YY45 pKa = 9.88DD46 pKa = 3.09DD47 pKa = 3.55WSWPKK52 pKa = 11.01GKK54 pKa = 9.5IDD56 pKa = 4.39DD57 pKa = 4.67GEE59 pKa = 4.58TIPEE63 pKa = 4.04CALRR67 pKa = 11.84EE68 pKa = 3.99VRR70 pKa = 11.84EE71 pKa = 4.3EE72 pKa = 4.02IGLDD76 pKa = 3.39APLGRR81 pKa = 11.84PLPAIHH87 pKa = 6.16YY88 pKa = 8.76RR89 pKa = 11.84VASGLKK95 pKa = 9.62VVFYY99 pKa = 9.98WAVKK103 pKa = 10.22ANGTRR108 pKa = 11.84LVPDD112 pKa = 3.97GKK114 pKa = 10.86EE115 pKa = 3.7VDD117 pKa = 3.81SVMWCAPEE125 pKa = 4.02KK126 pKa = 10.36AAKK129 pKa = 10.14LLSNPTDD136 pKa = 3.78VVPLEE141 pKa = 4.31YY142 pKa = 10.23LRR144 pKa = 11.84KK145 pKa = 8.91AHH147 pKa = 7.16EE148 pKa = 4.42EE149 pKa = 4.49GEE151 pKa = 4.45LNTWPLVSSGMPKK164 pKa = 9.71RR165 pKa = 11.84SHH167 pKa = 6.49ARR169 pKa = 11.84PGPKK173 pKa = 9.55PKK175 pKa = 10.57VNARR179 pKa = 11.84WPPPAPGRR187 pKa = 11.84HH188 pKa = 5.22RR189 pKa = 11.84RR190 pKa = 11.84WDD192 pKa = 3.38GCCRR196 pKa = 11.84YY197 pKa = 9.91GSPRR201 pKa = 11.84AWSPVPGSGAWRR213 pKa = 11.84LLHH216 pKa = 7.2PMRR219 pKa = 11.84KK220 pKa = 7.89WQAQRR225 pKa = 11.84FGWRR229 pKa = 11.84KK230 pKa = 8.67PSRR233 pKa = 11.84NTATTAARR241 pKa = 11.84RR242 pKa = 11.84RR243 pKa = 11.84PRR245 pKa = 11.84PSSRR249 pKa = 11.84PSSISSGRR257 pKa = 11.84WWSAPTAPRR266 pKa = 11.84CRR268 pKa = 11.84RR269 pKa = 11.84SSRR272 pKa = 11.84SWRR275 pKa = 11.84STCPAGWAACCPRR288 pKa = 11.84RR289 pKa = 11.84SHH291 pKa = 7.32ISPPANWWSAMWRR304 pKa = 11.84PGQRR308 pKa = 11.84TGWSPSSSSSRR319 pKa = 11.84STTDD323 pKa = 3.12PAGIQEE329 pKa = 4.44ADD331 pKa = 3.31KK332 pKa = 11.09QPRR335 pKa = 3.27

Molecular weight: 37.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3633

0

3633

1041394

20

1758

286.6

30.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.219 ± 0.058

0.652 ± 0.011

5.587 ± 0.034

5.651 ± 0.04

3.2 ± 0.027

9.163 ± 0.036

2.114 ± 0.021

4.197 ± 0.027

2.719 ± 0.03

10.242 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.997 ± 0.017

2.427 ± 0.023

5.632 ± 0.04

3.194 ± 0.022

6.619 ± 0.043

5.783 ± 0.03

5.796 ± 0.033

8.268 ± 0.036

1.47 ± 0.018

2.071 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski