Fowl aviadenovirus 6
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A191ULR9|A0A191ULR9_9ADEN Penton protein OS=Fowl aviadenovirus 6 OX=172862 GN=L2 PE=2 SV=1
MM1 pKa = 7.12 ATSTPHH7 pKa = 6.67 AFSFGQIGSRR17 pKa = 11.84 KK18 pKa = 9.34 RR19 pKa = 11.84 PAGGDD24 pKa = 3.67 GEE26 pKa = 4.79 RR27 pKa = 11.84 DD28 pKa = 3.22 ASKK31 pKa = 10.42 VPKK34 pKa = 8.97 MQTPAPSATANGNDD48 pKa = 4.13 EE49 pKa = 5.04 LDD51 pKa = 3.4 LVYY54 pKa = 10.27 PFWLQNGSTGGGGGGGSGGNPSLNPPFLDD83 pKa = 3.61 PNGPLAVQNNLLKK96 pKa = 11.07 VNTAAPITVANKK108 pKa = 10.48 AITLAYY114 pKa = 10.26 DD115 pKa = 3.61 PEE117 pKa = 4.37 SLEE120 pKa = 5.58 LNNQQQLSVKK130 pKa = 9.59 IDD132 pKa = 3.39 PEE134 pKa = 5.14 GPLKK138 pKa = 9.55 PTADD142 pKa = 5.01 GIQLSVDD149 pKa = 3.44 PTTLEE154 pKa = 3.5 VDD156 pKa = 3.24 ADD158 pKa = 3.8 DD159 pKa = 4.76 WEE161 pKa = 4.57 LTVNLDD167 pKa = 4.43 LNGPLDD173 pKa = 3.78 ATASGITVRR182 pKa = 11.84 VDD184 pKa = 3.02 DD185 pKa = 4.39 TLLIEE190 pKa = 6.01 DD191 pKa = 5.13 DD192 pKa = 5.2 DD193 pKa = 4.7 SGQGKK198 pKa = 8.91 EE199 pKa = 4.5 LGVNLNPAGPITADD213 pKa = 3.33 QNGLDD218 pKa = 5.32 LEE220 pKa = 5.95 IDD222 pKa = 3.82 DD223 pKa = 4.21 QTLKK227 pKa = 11.0 VNSGTSGGVLAVQIKK242 pKa = 8.81 PQGGLTSQTDD252 pKa = 5.22 GIQVSTQNSITLNNGALDD270 pKa = 3.92 VKK272 pKa = 10.74 VVADD276 pKa = 4.45 GPLASTANGLTLNYY290 pKa = 9.83 DD291 pKa = 3.5 PSDD294 pKa = 3.54 FTVNDD299 pKa = 3.85 AGTLSILRR307 pKa = 11.84 NPSVVANAYY316 pKa = 7.22 LTSGASTLQQFTAQGEE332 pKa = 4.3 NSSQFSFPCAYY343 pKa = 10.45 YY344 pKa = 9.99 LQQWLSDD351 pKa = 3.44 GLIFSSLYY359 pKa = 10.6 LKK361 pKa = 10.49 LDD363 pKa = 3.79 RR364 pKa = 11.84 AHH366 pKa = 6.13 FTHH369 pKa = 6.32 MQTGDD374 pKa = 2.99 TYY376 pKa = 11.7 QNAKK380 pKa = 10.5 YY381 pKa = 8.31 FTFWVGAGTSFNLSTLTVPTITPNTTQWNAFEE413 pKa = 4.54 PALDD417 pKa = 3.89 YY418 pKa = 11.51 SGAPPFDD425 pKa = 3.92 YY426 pKa = 10.94 LSTSVLTIYY435 pKa = 10.58 FEE437 pKa = 4.28 PTTGRR442 pKa = 11.84 LEE444 pKa = 4.18 SYY446 pKa = 10.9 LPVLTGNWSQTPYY459 pKa = 11.18 NPGTITLCVRR469 pKa = 11.84 AVRR472 pKa = 11.84 VQSRR476 pKa = 11.84 SQQTFSTLVCYY487 pKa = 10.25 NFRR490 pKa = 11.84 CQNAGIFNSNATAGTMTLGPIFFSCPAVSTVNAPP524 pKa = 3.17
Molecular weight: 55.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.136
IPC2_protein 4.126
IPC_protein 4.139
Toseland 3.923
ProMoST 4.304
Dawson 4.126
Bjellqvist 4.279
Wikipedia 4.062
Rodwell 3.961
Grimsley 3.821
Solomon 4.126
Lehninger 4.088
Nozaki 4.24
DTASelect 4.507
Thurlkill 3.961
EMBOSS 4.075
Sillero 4.266
Patrickios 3.261
IPC_peptide 4.126
IPC2_peptide 4.24
IPC2.peptide.svr19 4.185
Protein with the highest isoelectric point:
>tr|A0A191ULS6|A0A191ULS6_9ADEN ORF17 OS=Fowl aviadenovirus 6 OX=172862 PE=4 SV=1
MM1 pKa = 7.68 SILISPNDD9 pKa = 3.25 NRR11 pKa = 11.84 GWGMRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 SRR20 pKa = 11.84 SSMRR24 pKa = 11.84 GVGIRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 LTLRR36 pKa = 11.84 SLLGLGTVSRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 GGRR52 pKa = 11.84 SSRR55 pKa = 11.84 RR56 pKa = 11.84 SSRR59 pKa = 11.84 PASTTTRR66 pKa = 11.84 LMVVRR71 pKa = 11.84 TSRR74 pKa = 11.84 RR75 pKa = 11.84 RR76 pKa = 11.84 RR77 pKa = 11.84 RR78 pKa = 3.32
Molecular weight: 9.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.574
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.296
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.258
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
36
1
37
12920
54
1305
349.2
39.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.274 ± 0.437
1.827 ± 0.205
5.704 ± 0.273
6.099 ± 0.442
4.149 ± 0.238
6.378 ± 0.322
2.337 ± 0.192
3.576 ± 0.155
3.46 ± 0.273
8.994 ± 0.344
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.276 ± 0.172
3.916 ± 0.401
7.036 ± 0.302
3.692 ± 0.264
7.67 ± 0.453
6.966 ± 0.368
5.859 ± 0.36
6.61 ± 0.289
1.37 ± 0.116
3.808 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here