Gordonia phage Emalyn

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Emalynvirus; Gordonia virus Emalyn

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142KBW5|A0A142KBW5_9CAUD Uncharacterized protein OS=Gordonia phage Emalyn OX=1821552 GN=29 PE=4 SV=1
MM1 pKa = 6.26STRR4 pKa = 11.84YY5 pKa = 9.59ISLIADD11 pKa = 3.42NDD13 pKa = 3.89TCRR16 pKa = 11.84QAAGEE21 pKa = 3.98AAQRR25 pKa = 11.84IHH27 pKa = 7.58DD28 pKa = 4.36GADD31 pKa = 3.15HH32 pKa = 7.21DD33 pKa = 4.72EE34 pKa = 4.45VVADD38 pKa = 4.0LAVDD42 pKa = 3.87FFYY45 pKa = 9.8ITHH48 pKa = 7.24RR49 pKa = 11.84AMGMADD55 pKa = 4.08DD56 pKa = 5.61EE57 pKa = 4.72IDD59 pKa = 4.2NVWEE63 pKa = 4.2SDD65 pKa = 3.06WEE67 pKa = 4.09AFEE70 pKa = 6.38DD71 pKa = 3.97DD72 pKa = 4.13WLCAAEE78 pKa = 4.73DD79 pKa = 3.89ALDD82 pKa = 3.4

Molecular weight: 9.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142KBT9|A0A142KBT9_9CAUD Terminase large subunit OS=Gordonia phage Emalyn OX=1821552 GN=3 PE=4 SV=1
MM1 pKa = 7.08ARR3 pKa = 11.84KK4 pKa = 9.64RR5 pKa = 11.84KK6 pKa = 9.36GFRR9 pKa = 11.84SKK11 pKa = 10.78KK12 pKa = 5.72QWRR15 pKa = 11.84WAFATKK21 pKa = 10.3QPWARR26 pKa = 11.84RR27 pKa = 11.84WAHH30 pKa = 4.11QTKK33 pKa = 10.04GGKK36 pKa = 6.45KK37 pKa = 6.86TRR39 pKa = 11.84YY40 pKa = 9.21RR41 pKa = 11.84RR42 pKa = 11.84LPTRR46 pKa = 11.84KK47 pKa = 9.51KK48 pKa = 9.78ARR50 pKa = 11.84KK51 pKa = 8.82RR52 pKa = 3.41

Molecular weight: 6.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

14186

31

1570

211.7

23.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.003 ± 0.565

0.874 ± 0.153

6.38 ± 0.396

6.274 ± 0.397

3.095 ± 0.206

8.269 ± 0.454

2.122 ± 0.198

4.772 ± 0.234

5.054 ± 0.285

8.057 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.742 ± 0.153

3.13 ± 0.196

5.386 ± 0.199

3.539 ± 0.207

6.499 ± 0.384

6.224 ± 0.268

5.801 ± 0.244

6.64 ± 0.237

2.185 ± 0.133

2.954 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski