Amino acid dipepetide frequency for Gordonia phage Emalyn

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.28AlaAla: 11.28 ± 1.736
0.423AlaCys: 0.423 ± 0.182
6.133AlaAsp: 6.133 ± 0.878
5.922AlaGlu: 5.922 ± 0.779
3.595AlaPhe: 3.595 ± 0.719
8.601AlaGly: 8.601 ± 1.004
1.48AlaHis: 1.48 ± 0.302
5.217AlaIle: 5.217 ± 0.87
4.371AlaLys: 4.371 ± 0.79
9.024AlaLeu: 9.024 ± 0.912
2.608AlaMet: 2.608 ± 0.373
2.749AlaAsn: 2.749 ± 0.434
5.781AlaPro: 5.781 ± 0.622
2.679AlaGln: 2.679 ± 0.347
6.909AlaArg: 6.909 ± 0.846
6.345AlaSer: 6.345 ± 0.771
6.063AlaThr: 6.063 ± 0.726
5.992AlaVal: 5.992 ± 0.88
2.397AlaTrp: 2.397 ± 0.418
2.467AlaTyr: 2.467 ± 0.402
0.0AlaXaa: 0.0 ± 0.0
Cys
0.846CysAla: 0.846 ± 0.339
0.141CysCys: 0.141 ± 0.109
0.564CysAsp: 0.564 ± 0.234
0.705CysGlu: 0.705 ± 0.24
0.423CysPhe: 0.423 ± 0.171
0.846CysGly: 0.846 ± 0.27
0.211CysHis: 0.211 ± 0.124
0.211CysIle: 0.211 ± 0.119
0.282CysLys: 0.282 ± 0.147
0.493CysLeu: 0.493 ± 0.185
0.141CysMet: 0.141 ± 0.104
0.141CysAsn: 0.141 ± 0.09
0.564CysPro: 0.564 ± 0.21
0.211CysGln: 0.211 ± 0.128
0.564CysArg: 0.564 ± 0.216
0.564CysSer: 0.564 ± 0.214
0.634CysThr: 0.634 ± 0.203
0.493CysVal: 0.493 ± 0.215
0.211CysTrp: 0.211 ± 0.099
0.493CysTyr: 0.493 ± 0.176
0.0CysXaa: 0.0 ± 0.0
Asp
6.063AspAla: 6.063 ± 0.595
0.634AspCys: 0.634 ± 0.259
3.948AspAsp: 3.948 ± 0.728
5.71AspGlu: 5.71 ± 0.84
2.185AspPhe: 2.185 ± 0.451
4.371AspGly: 4.371 ± 0.53
1.551AspHis: 1.551 ± 0.362
3.243AspIle: 3.243 ± 0.497
2.397AspLys: 2.397 ± 0.381
5.992AspLeu: 5.992 ± 0.888
1.833AspMet: 1.833 ± 0.429
1.903AspAsn: 1.903 ± 0.351
3.807AspPro: 3.807 ± 0.561
1.762AspGln: 1.762 ± 0.373
3.595AspArg: 3.595 ± 0.369
4.3AspSer: 4.3 ± 0.603
3.172AspThr: 3.172 ± 0.496
3.243AspVal: 3.243 ± 0.717
1.692AspTrp: 1.692 ± 0.357
2.397AspTyr: 2.397 ± 0.403
0.0AspXaa: 0.0 ± 0.0
Glu
6.697GluAla: 6.697 ± 0.775
0.705GluCys: 0.705 ± 0.229
6.133GluAsp: 6.133 ± 0.9
4.794GluGlu: 4.794 ± 0.782
1.48GluPhe: 1.48 ± 0.276
4.018GluGly: 4.018 ± 0.399
1.551GluHis: 1.551 ± 0.345
2.256GluIle: 2.256 ± 0.338
2.467GluLys: 2.467 ± 0.524
5.569GluLeu: 5.569 ± 0.736
1.48GluMet: 1.48 ± 0.352
1.551GluAsn: 1.551 ± 0.306
2.608GluPro: 2.608 ± 0.427
2.467GluGln: 2.467 ± 0.478
4.512GluArg: 4.512 ± 0.725
3.313GluSer: 3.313 ± 0.445
2.538GluThr: 2.538 ± 0.457
4.723GluVal: 4.723 ± 0.702
1.339GluTrp: 1.339 ± 0.297
2.538GluTyr: 2.538 ± 0.501
0.0GluXaa: 0.0 ± 0.0
Phe
2.82PheAla: 2.82 ± 0.468
0.423PheCys: 0.423 ± 0.195
2.608PheAsp: 2.608 ± 0.455
1.692PheGlu: 1.692 ± 0.307
0.775PhePhe: 0.775 ± 0.226
2.326PheGly: 2.326 ± 0.411
0.775PheHis: 0.775 ± 0.259
2.185PheIle: 2.185 ± 0.457
1.974PheLys: 1.974 ± 0.467
2.115PheLeu: 2.115 ± 0.378
0.564PheMet: 0.564 ± 0.162
1.057PheAsn: 1.057 ± 0.214
1.198PhePro: 1.198 ± 0.399
0.916PheGln: 0.916 ± 0.274
2.185PheArg: 2.185 ± 0.397
2.044PheSer: 2.044 ± 0.789
2.538PheThr: 2.538 ± 0.378
1.551PheVal: 1.551 ± 0.322
0.493PheTrp: 0.493 ± 0.164
0.705PheTyr: 0.705 ± 0.242
0.0PheXaa: 0.0 ± 0.0
Gly
7.684GlyAla: 7.684 ± 0.887
0.634GlyCys: 0.634 ± 0.234
5.922GlyAsp: 5.922 ± 0.768
4.935GlyGlu: 4.935 ± 0.476
2.961GlyPhe: 2.961 ± 0.474
6.909GlyGly: 6.909 ± 0.939
1.551GlyHis: 1.551 ± 0.32
3.877GlyIle: 3.877 ± 0.558
4.653GlyLys: 4.653 ± 0.731
4.935GlyLeu: 4.935 ± 0.855
2.256GlyMet: 2.256 ± 0.351
2.538GlyAsn: 2.538 ± 0.49
3.384GlyPro: 3.384 ± 0.783
3.172GlyGln: 3.172 ± 0.61
5.781GlyArg: 5.781 ± 0.585
5.005GlySer: 5.005 ± 0.884
5.076GlyThr: 5.076 ± 0.62
5.992GlyVal: 5.992 ± 0.505
2.397GlyTrp: 2.397 ± 0.468
3.031GlyTyr: 3.031 ± 0.472
0.0GlyXaa: 0.0 ± 0.0
His
1.974HisAla: 1.974 ± 0.33
0.211HisCys: 0.211 ± 0.114
1.269HisAsp: 1.269 ± 0.247
1.269HisGlu: 1.269 ± 0.265
0.846HisPhe: 0.846 ± 0.316
1.269HisGly: 1.269 ± 0.354
0.423HisHis: 0.423 ± 0.166
0.987HisIle: 0.987 ± 0.338
0.916HisLys: 0.916 ± 0.261
2.044HisLeu: 2.044 ± 0.424
0.846HisMet: 0.846 ± 0.234
0.987HisAsn: 0.987 ± 0.22
1.551HisPro: 1.551 ± 0.361
0.916HisGln: 0.916 ± 0.216
1.339HisArg: 1.339 ± 0.275
1.269HisSer: 1.269 ± 0.305
1.692HisThr: 1.692 ± 0.301
0.916HisVal: 0.916 ± 0.221
0.211HisTrp: 0.211 ± 0.112
0.282HisTyr: 0.282 ± 0.113
0.0HisXaa: 0.0 ± 0.0
Ile
4.441IleAla: 4.441 ± 0.732
0.141IleCys: 0.141 ± 0.101
3.313IleAsp: 3.313 ± 0.427
3.313IleGlu: 3.313 ± 0.471
1.339IlePhe: 1.339 ± 0.299
3.666IleGly: 3.666 ± 0.56
0.916IleHis: 0.916 ± 0.258
2.256IleIle: 2.256 ± 0.607
2.679IleLys: 2.679 ± 0.626
2.82IleLeu: 2.82 ± 0.465
0.846IleMet: 0.846 ± 0.244
1.41IleAsn: 1.41 ± 0.302
2.961IlePro: 2.961 ± 0.412
1.339IleGln: 1.339 ± 0.278
3.525IleArg: 3.525 ± 0.508
3.525IleSer: 3.525 ± 0.709
2.961IleThr: 2.961 ± 0.443
3.595IleVal: 3.595 ± 0.455
1.057IleTrp: 1.057 ± 0.273
1.621IleTyr: 1.621 ± 0.448
0.0IleXaa: 0.0 ± 0.0
Lys
5.005LysAla: 5.005 ± 0.735
0.352LysCys: 0.352 ± 0.186
2.326LysAsp: 2.326 ± 0.413
3.243LysGlu: 3.243 ± 0.561
2.326LysPhe: 2.326 ± 0.421
4.23LysGly: 4.23 ± 0.584
1.057LysHis: 1.057 ± 0.315
1.762LysIle: 1.762 ± 0.387
2.89LysLys: 2.89 ± 0.544
4.864LysLeu: 4.864 ± 0.67
1.198LysMet: 1.198 ± 0.274
2.185LysAsn: 2.185 ± 0.403
2.397LysPro: 2.397 ± 0.412
2.679LysGln: 2.679 ± 0.513
3.243LysArg: 3.243 ± 0.476
2.89LysSer: 2.89 ± 0.458
2.82LysThr: 2.82 ± 0.48
2.82LysVal: 2.82 ± 0.498
0.846LysTrp: 0.846 ± 0.216
1.41LysTyr: 1.41 ± 0.364
0.0LysXaa: 0.0 ± 0.0
Leu
8.601LeuAla: 8.601 ± 0.988
0.846LeuCys: 0.846 ± 0.317
4.723LeuAsp: 4.723 ± 0.733
5.076LeuGlu: 5.076 ± 0.844
1.551LeuPhe: 1.551 ± 0.505
6.345LeuGly: 6.345 ± 0.834
1.692LeuHis: 1.692 ± 0.33
3.595LeuIle: 3.595 ± 0.497
4.653LeuLys: 4.653 ± 0.591
6.979LeuLeu: 6.979 ± 0.685
2.185LeuMet: 2.185 ± 0.372
2.608LeuAsn: 2.608 ± 0.473
4.864LeuPro: 4.864 ± 0.537
2.538LeuGln: 2.538 ± 0.428
6.345LeuArg: 6.345 ± 0.654
5.146LeuSer: 5.146 ± 0.679
4.23LeuThr: 4.23 ± 0.597
4.653LeuVal: 4.653 ± 0.562
1.551LeuTrp: 1.551 ± 0.422
2.326LeuTyr: 2.326 ± 0.358
0.0LeuXaa: 0.0 ± 0.0
Met
3.595MetAla: 3.595 ± 0.462
0.07MetCys: 0.07 ± 0.071
1.339MetAsp: 1.339 ± 0.34
1.339MetGlu: 1.339 ± 0.318
0.987MetPhe: 0.987 ± 0.233
1.269MetGly: 1.269 ± 0.256
0.564MetHis: 0.564 ± 0.22
1.903MetIle: 1.903 ± 0.347
1.269MetLys: 1.269 ± 0.249
2.115MetLeu: 2.115 ± 0.341
0.705MetMet: 0.705 ± 0.183
0.916MetAsn: 0.916 ± 0.203
1.339MetPro: 1.339 ± 0.25
1.269MetGln: 1.269 ± 0.249
1.198MetArg: 1.198 ± 0.271
2.326MetSer: 2.326 ± 0.46
1.903MetThr: 1.903 ± 0.304
2.115MetVal: 2.115 ± 0.397
0.705MetTrp: 0.705 ± 0.217
0.493MetTyr: 0.493 ± 0.174
0.0MetXaa: 0.0 ± 0.0
Asn
3.172AsnAla: 3.172 ± 0.536
0.352AsnCys: 0.352 ± 0.182
1.621AsnAsp: 1.621 ± 0.471
1.269AsnGlu: 1.269 ± 0.287
1.128AsnPhe: 1.128 ± 0.246
3.384AsnGly: 3.384 ± 0.557
0.634AsnHis: 0.634 ± 0.22
1.339AsnIle: 1.339 ± 0.588
1.48AsnLys: 1.48 ± 0.364
1.198AsnLeu: 1.198 ± 0.301
1.198AsnMet: 1.198 ± 0.26
0.634AsnAsn: 0.634 ± 0.279
3.454AsnPro: 3.454 ± 0.476
1.198AsnGln: 1.198 ± 0.233
2.608AsnArg: 2.608 ± 0.401
1.762AsnSer: 1.762 ± 0.336
1.339AsnThr: 1.339 ± 0.335
2.115AsnVal: 2.115 ± 0.343
0.634AsnTrp: 0.634 ± 0.168
0.775AsnTyr: 0.775 ± 0.21
0.0AsnXaa: 0.0 ± 0.0
Pro
5.922ProAla: 5.922 ± 0.643
0.634ProCys: 0.634 ± 0.267
4.582ProAsp: 4.582 ± 0.56
3.031ProGlu: 3.031 ± 0.588
1.621ProPhe: 1.621 ± 0.327
5.428ProGly: 5.428 ± 0.721
1.198ProHis: 1.198 ± 0.257
1.903ProIle: 1.903 ± 0.373
3.172ProLys: 3.172 ± 0.525
3.948ProLeu: 3.948 ± 0.529
1.198ProMet: 1.198 ± 0.305
1.833ProAsn: 1.833 ± 0.318
2.538ProPro: 2.538 ± 0.47
1.974ProGln: 1.974 ± 0.381
2.256ProArg: 2.256 ± 0.44
3.454ProSer: 3.454 ± 0.675
3.666ProThr: 3.666 ± 0.454
3.243ProVal: 3.243 ± 0.485
1.269ProTrp: 1.269 ± 0.276
0.987ProTyr: 0.987 ± 0.258
0.0ProXaa: 0.0 ± 0.0
Gln
3.243GlnAla: 3.243 ± 0.481
0.423GlnCys: 0.423 ± 0.164
1.903GlnAsp: 1.903 ± 0.362
2.185GlnGlu: 2.185 ± 0.399
1.551GlnPhe: 1.551 ± 0.376
2.608GlnGly: 2.608 ± 0.416
0.846GlnHis: 0.846 ± 0.268
1.974GlnIle: 1.974 ± 0.399
1.41GlnLys: 1.41 ± 0.305
3.102GlnLeu: 3.102 ± 0.404
0.916GlnMet: 0.916 ± 0.248
0.916GlnAsn: 0.916 ± 0.275
1.762GlnPro: 1.762 ± 0.357
1.551GlnGln: 1.551 ± 0.391
2.749GlnArg: 2.749 ± 0.363
2.538GlnSer: 2.538 ± 0.443
1.339GlnThr: 1.339 ± 0.312
2.538GlnVal: 2.538 ± 0.41
0.705GlnTrp: 0.705 ± 0.239
1.128GlnTyr: 1.128 ± 0.317
0.0GlnXaa: 0.0 ± 0.0
Arg
6.486ArgAla: 6.486 ± 0.781
0.493ArgCys: 0.493 ± 0.213
4.089ArgAsp: 4.089 ± 0.653
4.3ArgGlu: 4.3 ± 0.54
2.115ArgPhe: 2.115 ± 0.397
5.64ArgGly: 5.64 ± 0.641
0.987ArgHis: 0.987 ± 0.275
2.397ArgIle: 2.397 ± 0.375
4.441ArgLys: 4.441 ± 0.57
4.794ArgLeu: 4.794 ± 0.679
1.833ArgMet: 1.833 ± 0.509
1.621ArgAsn: 1.621 ± 0.314
2.326ArgPro: 2.326 ± 0.427
2.326ArgGln: 2.326 ± 0.33
4.794ArgArg: 4.794 ± 0.809
3.525ArgSer: 3.525 ± 0.525
3.384ArgThr: 3.384 ± 0.552
5.64ArgVal: 5.64 ± 0.646
1.762ArgTrp: 1.762 ± 0.461
2.044ArgTyr: 2.044 ± 0.42
0.0ArgXaa: 0.0 ± 0.0
Ser
6.133SerAla: 6.133 ± 0.655
0.282SerCys: 0.282 ± 0.161
2.961SerAsp: 2.961 ± 0.339
4.441SerGlu: 4.441 ± 0.793
1.339SerPhe: 1.339 ± 0.253
7.684SerGly: 7.684 ± 0.881
1.269SerHis: 1.269 ± 0.336
3.877SerIle: 3.877 ± 0.608
2.679SerLys: 2.679 ± 0.44
5.287SerLeu: 5.287 ± 0.67
1.762SerMet: 1.762 ± 0.356
2.044SerAsn: 2.044 ± 0.334
3.384SerPro: 3.384 ± 0.619
2.044SerGln: 2.044 ± 0.476
3.031SerArg: 3.031 ± 0.426
3.595SerSer: 3.595 ± 0.743
4.089SerThr: 4.089 ± 0.734
3.172SerVal: 3.172 ± 0.428
1.339SerTrp: 1.339 ± 0.299
1.833SerTyr: 1.833 ± 0.35
0.0SerXaa: 0.0 ± 0.0
Thr
4.935ThrAla: 4.935 ± 0.649
0.423ThrCys: 0.423 ± 0.202
3.102ThrAsp: 3.102 ± 0.612
2.397ThrGlu: 2.397 ± 0.42
1.269ThrPhe: 1.269 ± 0.29
5.569ThrGly: 5.569 ± 0.644
1.339ThrHis: 1.339 ± 0.321
2.961ThrIle: 2.961 ± 0.518
3.666ThrLys: 3.666 ± 0.66
4.864ThrLeu: 4.864 ± 0.606
1.903ThrMet: 1.903 ± 0.383
1.833ThrAsn: 1.833 ± 0.281
4.159ThrPro: 4.159 ± 0.523
2.044ThrGln: 2.044 ± 0.338
3.736ThrArg: 3.736 ± 0.555
3.102ThrSer: 3.102 ± 0.41
4.018ThrThr: 4.018 ± 0.676
4.371ThrVal: 4.371 ± 0.62
0.846ThrTrp: 0.846 ± 0.198
1.48ThrTyr: 1.48 ± 0.314
0.0ThrXaa: 0.0 ± 0.0
Val
7.05ValAla: 7.05 ± 0.819
0.775ValCys: 0.775 ± 0.309
4.089ValAsp: 4.089 ± 0.555
3.595ValGlu: 3.595 ± 0.645
1.833ValPhe: 1.833 ± 0.316
4.935ValGly: 4.935 ± 0.805
1.551ValHis: 1.551 ± 0.346
3.595ValIle: 3.595 ± 0.51
2.82ValLys: 2.82 ± 0.455
5.499ValLeu: 5.499 ± 0.726
1.903ValMet: 1.903 ± 0.333
2.397ValAsn: 2.397 ± 0.474
3.736ValPro: 3.736 ± 0.609
2.467ValGln: 2.467 ± 0.389
3.313ValArg: 3.313 ± 0.537
4.159ValSer: 4.159 ± 0.528
4.723ValThr: 4.723 ± 0.585
4.371ValVal: 4.371 ± 0.626
1.762ValTrp: 1.762 ± 0.328
1.833ValTyr: 1.833 ± 0.384
0.0ValXaa: 0.0 ± 0.0
Trp
2.397TrpAla: 2.397 ± 0.5
0.423TrpCys: 0.423 ± 0.165
1.269TrpAsp: 1.269 ± 0.27
1.198TrpGlu: 1.198 ± 0.267
0.493TrpPhe: 0.493 ± 0.195
1.198TrpGly: 1.198 ± 0.306
0.634TrpHis: 0.634 ± 0.202
0.423TrpIle: 0.423 ± 0.175
0.987TrpLys: 0.987 ± 0.231
2.467TrpLeu: 2.467 ± 0.385
0.634TrpMet: 0.634 ± 0.199
1.128TrpAsn: 1.128 ± 0.359
0.775TrpPro: 0.775 ± 0.224
1.198TrpGln: 1.198 ± 0.263
1.198TrpArg: 1.198 ± 0.274
1.551TrpSer: 1.551 ± 0.327
0.493TrpThr: 0.493 ± 0.16
2.115TrpVal: 2.115 ± 0.413
0.352TrpTrp: 0.352 ± 0.138
0.916TrpTyr: 0.916 ± 0.275
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.692TyrAla: 1.692 ± 0.314
0.352TyrCys: 0.352 ± 0.175
1.903TyrAsp: 1.903 ± 0.338
2.326TyrGlu: 2.326 ± 0.464
1.128TyrPhe: 1.128 ± 0.276
2.397TyrGly: 2.397 ± 0.411
0.987TyrHis: 0.987 ± 0.322
1.762TyrIle: 1.762 ± 0.339
1.339TyrLys: 1.339 ± 0.298
2.467TyrLeu: 2.467 ± 0.535
1.339TyrMet: 1.339 ± 0.253
0.846TyrAsn: 0.846 ± 0.246
1.269TyrPro: 1.269 ± 0.345
0.634TyrGln: 0.634 ± 0.199
2.115TyrArg: 2.115 ± 0.441
1.833TyrSer: 1.833 ± 0.354
1.339TyrThr: 1.339 ± 0.335
2.749TyrVal: 2.749 ± 0.648
0.282TyrTrp: 0.282 ± 0.138
0.775TyrTyr: 0.775 ± 0.287
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (14186 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski