Wallemia hederae
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4107 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4T0FCD5|A0A4T0FCD5_9BASI Aminotran_1_2 domain-containing protein OS=Wallemia hederae OX=1540922 GN=E3P99_03898 PE=4 SV=1
MM1 pKa = 7.03 VGKK4 pKa = 10.51 FGLLSLAAVAGVAAAQSKK22 pKa = 10.17 DD23 pKa = 3.49 YY24 pKa = 10.82 PGVVSANDD32 pKa = 3.59 NGPTNPDD39 pKa = 3.2 QPEE42 pKa = 4.58 LGTDD46 pKa = 3.41 TPEE49 pKa = 4.47 DD50 pKa = 3.61 SEE52 pKa = 5.14 ARR54 pKa = 11.84 LLTVNNIDD62 pKa = 4.06 DD63 pKa = 3.92 FCIFGPKK70 pKa = 10.09 DD71 pKa = 3.2 ADD73 pKa = 3.46 KK74 pKa = 10.98 TIGEE78 pKa = 4.44 TEE80 pKa = 4.03 AEE82 pKa = 4.08 QVAWCTQPRR91 pKa = 11.84 NNARR95 pKa = 11.84 LIPEE99 pKa = 3.97 DD100 pKa = 4.19 TFTGLHH106 pKa = 5.54 FVKK109 pKa = 9.94 TPYY112 pKa = 10.39 YY113 pKa = 9.96 IQLQGWGDD121 pKa = 3.56 LTKK124 pKa = 10.45 IGIKK128 pKa = 10.61 DD129 pKa = 3.23 GDD131 pKa = 3.9 YY132 pKa = 11.17 GGEE135 pKa = 4.14 LDD137 pKa = 3.57 PHH139 pKa = 5.94 GAKK142 pKa = 10.57 DD143 pKa = 3.69 LGNPIGGNVTSNISGEE159 pKa = 3.99 ATFYY163 pKa = 10.39 EE164 pKa = 4.5 EE165 pKa = 3.5 WMEE168 pKa = 4.49 FISYY172 pKa = 9.4 EE173 pKa = 4.14 QFCLRR178 pKa = 11.84 VCTAGDD184 pKa = 4.06 DD185 pKa = 3.68 TWDD188 pKa = 3.44 AATMCEE194 pKa = 4.08 HH195 pKa = 7.14 KK196 pKa = 10.25 LDD198 pKa = 3.76 EE199 pKa = 4.6 MGCGFIMPGDD209 pKa = 3.85 YY210 pKa = 9.93 QGKK213 pKa = 8.18 QDD215 pKa = 4.48 QFVSCDD221 pKa = 3.08 ADD223 pKa = 3.43 AAFPPGVYY231 pKa = 9.46 PDD233 pKa = 3.71 GDD235 pKa = 3.83 GYY237 pKa = 10.24 STFNQRR243 pKa = 11.84 YY244 pKa = 6.64 TGTLTSNTYY253 pKa = 9.38 EE254 pKa = 4.21 VYY256 pKa = 10.05 TVGDD260 pKa = 3.78 TATPSGPYY268 pKa = 8.6 STPSSSNCKK277 pKa = 8.08 TQDD280 pKa = 3.37 GLNTYY285 pKa = 10.28 VNTDD289 pKa = 3.22 APQSTGSSDD298 pKa = 3.38 NNTNAEE304 pKa = 4.25 NGDD307 pKa = 3.81 SGAGSVALGAVATVSIALISAFAILSS333 pKa = 3.64
Molecular weight: 35.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.773
IPC2_protein 3.859
IPC_protein 3.884
Toseland 3.656
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.706
Grimsley 3.567
Solomon 3.872
Lehninger 3.821
Nozaki 3.986
DTASelect 4.228
Thurlkill 3.706
EMBOSS 3.821
Sillero 3.999
Patrickios 0.604
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.893
Protein with the highest isoelectric point:
>tr|A0A4T0FKS1|A0A4T0FKS1_9BASI Isoleucyl-tRNA synthetase OS=Wallemia hederae OX=1540922 GN=E3P99_02380 PE=3 SV=1
MM1 pKa = 7.82 ASPATVFSPTLNALNQLRR19 pKa = 11.84 FASRR23 pKa = 11.84 GTDD26 pKa = 3.54 YY27 pKa = 11.19 QPSTRR32 pKa = 11.84 KK33 pKa = 9.59 RR34 pKa = 11.84 KK35 pKa = 8.51 RR36 pKa = 11.84 TFGFLARR43 pKa = 11.84 IRR45 pKa = 11.84 SRR47 pKa = 11.84 TGRR50 pKa = 11.84 KK51 pKa = 9.05 IIARR55 pKa = 11.84 RR56 pKa = 11.84 LKK58 pKa = 10.34 RR59 pKa = 11.84 GRR61 pKa = 11.84 KK62 pKa = 8.89 NMSHH66 pKa = 6.79
Molecular weight: 7.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.371
IPC2_protein 10.935
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.193
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.93
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.094
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4107
0
4107
2297997
59
4344
559.5
62.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.12 ± 0.031
1.029 ± 0.012
6.308 ± 0.027
6.179 ± 0.034
3.578 ± 0.022
5.863 ± 0.031
2.597 ± 0.017
5.157 ± 0.024
5.862 ± 0.031
9.168 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.056 ± 0.012
4.381 ± 0.02
5.143 ± 0.032
4.548 ± 0.034
5.422 ± 0.025
8.784 ± 0.038
5.748 ± 0.02
6.07 ± 0.028
1.101 ± 0.011
2.887 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here