Tyto alba (Barn owl)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria;

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9263 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A093F8X1|A0A093F8X1_TYTAL Replication protein A 14 kDa subunit (Fragment) OS=Tyto alba OX=56313 GN=N341_11129 PE=3 SV=1
MM1 pKa = 6.61TTGDD5 pKa = 3.89VLDD8 pKa = 4.17SCTAAEE14 pKa = 4.36LRR16 pKa = 11.84LDD18 pKa = 3.57SLYY21 pKa = 10.45LQRR24 pKa = 11.84MDD26 pKa = 3.06SHH28 pKa = 6.85KK29 pKa = 11.28ADD31 pKa = 3.85VLDD34 pKa = 3.89VLPKK38 pKa = 10.29QEE40 pKa = 4.48IQSSDD45 pKa = 3.5CQAAASDD52 pKa = 5.27AEE54 pKa = 4.46VQDD57 pKa = 3.86PAKK60 pKa = 10.68GFLGHH65 pKa = 5.7YY66 pKa = 5.19TTPKK70 pKa = 10.16VVLSADD76 pKa = 3.56APQTEE81 pKa = 4.25ATEE84 pKa = 4.56CEE86 pKa = 4.3VGLPDD91 pKa = 4.69TYY93 pKa = 11.13LSPAADD99 pKa = 3.36SCEE102 pKa = 3.82NMSLATTDD110 pKa = 4.45KK111 pKa = 11.08GDD113 pKa = 3.78LPHH116 pKa = 7.51SIVYY120 pKa = 10.04QNEE123 pKa = 3.61EE124 pKa = 4.41GKK126 pKa = 9.57WVTDD130 pKa = 3.51LAYY133 pKa = 9.07YY134 pKa = 10.17TSFDD138 pKa = 3.77EE139 pKa = 4.71EE140 pKa = 3.89QDD142 pKa = 3.93LNLSEE147 pKa = 5.07DD148 pKa = 3.61DD149 pKa = 4.97KK150 pKa = 11.59INEE153 pKa = 4.8FITT156 pKa = 3.67

Molecular weight: 17.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A093F4G9|A0A093F4G9_TYTAL Katanin p60 ATPase-containing subunit A-like 1 (Fragment) OS=Tyto alba OX=56313 GN=N341_10014 PE=3 SV=1
MM1 pKa = 7.67AAQSAPKK8 pKa = 10.16VVLKK12 pKa = 10.04STTKK16 pKa = 9.35MSLNEE21 pKa = 4.25RR22 pKa = 11.84FTNMLKK28 pKa = 10.36NKK30 pKa = 9.73QPMPVNIRR38 pKa = 11.84ATMQQQQQLASARR51 pKa = 11.84NRR53 pKa = 11.84RR54 pKa = 11.84LAQQMEE60 pKa = 4.32NRR62 pKa = 11.84PSVQAALKK70 pKa = 10.37LKK72 pKa = 10.39QKK74 pKa = 9.42SLKK77 pKa = 9.72QRR79 pKa = 11.84LGKK82 pKa = 10.77SNIQARR88 pKa = 11.84LGRR91 pKa = 11.84PAGPLARR98 pKa = 11.84GAMGGRR104 pKa = 11.84GLSMGQRR111 pKa = 11.84GLPRR115 pKa = 11.84GAMRR119 pKa = 11.84GGRR122 pKa = 11.84GARR125 pKa = 11.84ALLRR129 pKa = 11.84GGVPLRR135 pKa = 11.84GQQ137 pKa = 3.17

Molecular weight: 14.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9263

0

9263

3777475

31

4763

407.8

45.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.212 ± 0.023

2.218 ± 0.019

5.064 ± 0.017

7.227 ± 0.034

4.003 ± 0.02

5.716 ± 0.033

2.536 ± 0.013

5.155 ± 0.019

6.628 ± 0.03

9.759 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.278 ± 0.011

4.258 ± 0.016

5.106 ± 0.031

4.655 ± 0.022

5.081 ± 0.018

8.185 ± 0.037

5.411 ± 0.018

6.281 ± 0.022

1.188 ± 0.009

3.034 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski