Amino acid dipepetide frequency for Tyto alba (Barn owl)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.351AlaAla: 5.351 ± 0.053	1.277AlaCys: 1.277 ± 0.025	2.959AlaAsp: 2.959 ± 0.03	4.503AlaGlu: 4.503 ± 0.042	2.625AlaPhe: 2.625 ± 0.029	3.63AlaGly: 3.63 ± 0.043	1.324AlaHis: 1.324 ± 0.019	3.144AlaIle: 3.144 ± 0.031	3.758AlaLys: 3.758 ± 0.04	6.273AlaLeu: 6.273 ± 0.056	1.457AlaMet: 1.457 ± 0.025	2.279AlaAsn: 2.279 ± 0.026	2.689AlaPro: 2.689 ± 0.033	2.652AlaGln: 2.652 ± 0.029	2.807AlaArg: 2.807 ± 0.032	4.982AlaSer: 4.982 ± 0.044	3.269AlaThr: 3.269 ± 0.034	4.795AlaVal: 4.795 ± 0.038	0.681AlaTrp: 0.681 ± 0.014	1.661AlaTyr: 1.661 ± 0.024	0.001AlaXaa: 0.001 ± 0.001
Cys	1.151CysAla: 1.151 ± 0.019	0.65CysCys: 0.65 ± 0.016	1.03CysAsp: 1.03 ± 0.02	1.293CysGlu: 1.293 ± 0.026	0.962CysPhe: 0.962 ± 0.019	1.406CysGly: 1.406 ± 0.027	0.635CysHis: 0.635 ± 0.015	1.183CysIle: 1.183 ± 0.023	1.358CysLys: 1.358 ± 0.023	2.221CysLeu: 2.221 ± 0.035	0.468CysMet: 0.468 ± 0.012	0.938CysAsn: 0.938 ± 0.018	1.162CysPro: 1.162 ± 0.03	1.079CysGln: 1.079 ± 0.022	1.168CysArg: 1.168 ± 0.021	1.98CysSer: 1.98 ± 0.028	1.187CysThr: 1.187 ± 0.021	1.333CysVal: 1.333 ± 0.024	0.29CysTrp: 0.29 ± 0.009	0.686CysTyr: 0.686 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	2.849AspAla: 2.849 ± 0.025	1.094AspCys: 1.094 ± 0.02	2.92AspAsp: 2.92 ± 0.033	3.69AspGlu: 3.69 ± 0.037	2.303AspPhe: 2.303 ± 0.024	3.167AspGly: 3.167 ± 0.033	1.218AspHis: 1.218 ± 0.02	3.104AspIle: 3.104 ± 0.033	2.913AspLys: 2.913 ± 0.033	5.141AspLeu: 5.141 ± 0.041	1.174AspMet: 1.174 ± 0.017	1.972AspAsn: 1.972 ± 0.027	2.609AspPro: 2.609 ± 0.03	1.881AspGln: 1.881 ± 0.025	2.357AspArg: 2.357 ± 0.026	4.113AspSer: 4.113 ± 0.043	2.554AspThr: 2.554 ± 0.026	3.234AspVal: 3.234 ± 0.031	0.651AspTrp: 0.651 ± 0.014	1.69AspTyr: 1.69 ± 0.023	0.001AspXaa: 0.001 ± 0.0
Glu	4.59GluAla: 4.59 ± 0.04	1.321GluCys: 1.321 ± 0.031	4.573GluAsp: 4.573 ± 0.046	7.864GluGlu: 7.864 ± 0.083	2.303GluPhe: 2.303 ± 0.028	3.885GluGly: 3.885 ± 0.035	1.564GluHis: 1.564 ± 0.023	3.706GluIle: 3.706 ± 0.04	5.937GluLys: 5.937 ± 0.061	6.4GluLeu: 6.4 ± 0.056	1.852GluMet: 1.852 ± 0.021	3.63GluAsn: 3.63 ± 0.037	2.453GluPro: 2.453 ± 0.032	3.21GluGln: 3.21 ± 0.033	3.828GluArg: 3.828 ± 0.041	4.6GluSer: 4.6 ± 0.041	3.604GluThr: 3.604 ± 0.032	4.364GluVal: 4.364 ± 0.038	0.733GluTrp: 0.733 ± 0.015	1.857GluTyr: 1.857 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	2.127PheAla: 2.127 ± 0.028	1.033PheCys: 1.033 ± 0.018	1.876PheAsp: 1.876 ± 0.022	2.223PheGlu: 2.223 ± 0.029	1.957PhePhe: 1.957 ± 0.028	2.307PheGly: 2.307 ± 0.029	1.129PheHis: 1.129 ± 0.02	2.23PheIle: 2.23 ± 0.032	2.255PheLys: 2.255 ± 0.026	4.371PheLeu: 4.371 ± 0.045	0.836PheMet: 0.836 ± 0.016	1.589PheAsn: 1.589 ± 0.021	1.968PhePro: 1.968 ± 0.032	1.877PheGln: 1.877 ± 0.021	1.949PheArg: 1.949 ± 0.026	3.569PheSer: 3.569 ± 0.036	2.325PheThr: 2.325 ± 0.027	2.475PheVal: 2.475 ± 0.032	0.541PheTrp: 0.541 ± 0.014	1.388PheTyr: 1.388 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	3.294GlyAla: 3.294 ± 0.035	1.188GlyCys: 1.188 ± 0.02	2.9GlyAsp: 2.9 ± 0.033	3.681GlyGlu: 3.681 ± 0.045	2.531GlyPhe: 2.531 ± 0.033	3.669GlyGly: 3.669 ± 0.056	1.466GlyHis: 1.466 ± 0.024	3.2GlyIle: 3.2 ± 0.033	3.989GlyLys: 3.989 ± 0.041	5.056GlyLeu: 5.056 ± 0.053	1.367GlyMet: 1.367 ± 0.023	2.632GlyAsn: 2.632 ± 0.035	2.595GlyPro: 2.595 ± 0.068	2.407GlyGln: 2.407 ± 0.03	3.049GlyArg: 3.049 ± 0.04	4.827GlySer: 4.827 ± 0.049	3.317GlyThr: 3.317 ± 0.04	3.389GlyVal: 3.389 ± 0.035	0.742GlyTrp: 0.742 ± 0.017	1.859GlyTyr: 1.859 ± 0.028	0.001GlyXaa: 0.001 ± 0.0
His	1.296HisAla: 1.296 ± 0.021	0.719HisCys: 0.719 ± 0.014	0.928HisAsp: 0.928 ± 0.017	1.378HisGlu: 1.378 ± 0.02	1.119HisPhe: 1.119 ± 0.017	1.456HisGly: 1.456 ± 0.022	0.848HisHis: 0.848 ± 0.017	1.384HisIle: 1.384 ± 0.019	1.457HisLys: 1.457 ± 0.024	2.83HisLeu: 2.83 ± 0.031	0.606HisMet: 0.606 ± 0.013	1.012HisAsn: 1.012 ± 0.016	1.409HisPro: 1.409 ± 0.022	1.194HisGln: 1.194 ± 0.02	1.409HisArg: 1.409 ± 0.022	2.193HisSer: 2.193 ± 0.026	1.315HisThr: 1.315 ± 0.021	1.534HisVal: 1.534 ± 0.023	0.407HisTrp: 0.407 ± 0.011	0.863HisTyr: 0.863 ± 0.017	0.001HisXaa: 0.001 ± 0.001
Ile	3.082IleAla: 3.082 ± 0.029	1.285IleCys: 1.285 ± 0.025	2.402IleAsp: 2.402 ± 0.026	3.041IleGlu: 3.041 ± 0.034	2.307IlePhe: 2.307 ± 0.029	2.577IleGly: 2.577 ± 0.027	1.456IleHis: 1.456 ± 0.02	2.938IleIle: 2.938 ± 0.034	3.244IleLys: 3.244 ± 0.032	5.166IleLeu: 5.166 ± 0.047	1.118IleMet: 1.118 ± 0.019	2.289IleAsn: 2.289 ± 0.024	2.898IlePro: 2.898 ± 0.035	2.554IleGln: 2.554 ± 0.032	2.601IleArg: 2.601 ± 0.027	4.277IleSer: 4.277 ± 0.042	2.979IleThr: 2.979 ± 0.033	3.028IleVal: 3.028 ± 0.031	0.612IleTrp: 0.612 ± 0.014	1.697IleTyr: 1.697 ± 0.024	0.0IleXaa: 0.0 ± 0.0
Lys	4.191LysAla: 4.191 ± 0.04	1.261LysCys: 1.261 ± 0.022	3.57LysAsp: 3.57 ± 0.034	5.974LysGlu: 5.974 ± 0.064	2.053LysPhe: 2.053 ± 0.023	3.501LysGly: 3.501 ± 0.051	1.67LysHis: 1.67 ± 0.023	3.393LysIle: 3.393 ± 0.033	5.663LysLys: 5.663 ± 0.057	6.055LysLeu: 6.055 ± 0.048	1.621LysMet: 1.621 ± 0.023	2.958LysAsn: 2.958 ± 0.029	3.091LysPro: 3.091 ± 0.04	3.115LysGln: 3.115 ± 0.038	3.581LysArg: 3.581 ± 0.034	4.427LysSer: 4.427 ± 0.049	3.566LysThr: 3.566 ± 0.028	3.889LysVal: 3.889 ± 0.039	0.706LysTrp: 0.706 ± 0.014	1.992LysTyr: 1.992 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	6.037LeuAla: 6.037 ± 0.056	2.196LeuCys: 2.196 ± 0.031	4.947LeuAsp: 4.947 ± 0.042	7.086LeuGlu: 7.086 ± 0.065	3.718LeuPhe: 3.718 ± 0.046	5.07LeuGly: 5.07 ± 0.048	2.703LeuHis: 2.703 ± 0.028	4.475LeuIle: 4.475 ± 0.043	6.771LeuLys: 6.771 ± 0.058	10.052LeuLeu: 10.052 ± 0.084	2.086LeuMet: 2.086 ± 0.028	4.055LeuAsn: 4.055 ± 0.033	5.321LeuPro: 5.321 ± 0.051	5.619LeuGln: 5.619 ± 0.047	4.987LeuArg: 4.987 ± 0.042	7.912LeuSer: 7.912 ± 0.054	5.112LeuThr: 5.112 ± 0.042	5.464LeuVal: 5.464 ± 0.045	1.076LeuTrp: 1.076 ± 0.019	2.898LeuTyr: 2.898 ± 0.03	0.002LeuXaa: 0.002 ± 0.001
Met	1.624MetAla: 1.624 ± 0.023	0.458MetCys: 0.458 ± 0.011	1.304MetAsp: 1.304 ± 0.02	1.935MetGlu: 1.935 ± 0.024	0.874MetPhe: 0.874 ± 0.016	1.263MetGly: 1.263 ± 0.02	0.526MetHis: 0.526 ± 0.012	1.035MetIle: 1.035 ± 0.017	1.683MetLys: 1.683 ± 0.02	2.12MetLeu: 2.12 ± 0.026	0.64MetMet: 0.64 ± 0.015	1.055MetAsn: 1.055 ± 0.019	1.021MetPro: 1.021 ± 0.016	1.039MetGln: 1.039 ± 0.018	1.071MetArg: 1.071 ± 0.017	1.565MetSer: 1.565 ± 0.02	1.186MetThr: 1.186 ± 0.019	1.441MetVal: 1.441 ± 0.02	0.258MetTrp: 0.258 ± 0.008	0.686MetTyr: 0.686 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.368AsnAla: 2.368 ± 0.027	1.013AsnCys: 1.013 ± 0.018	1.764AsnAsp: 1.764 ± 0.022	2.732AsnGlu: 2.732 ± 0.026	1.714AsnPhe: 1.714 ± 0.025	2.769AsnGly: 2.769 ± 0.037	1.035AsnHis: 1.035 ± 0.017	2.632AsnIle: 2.632 ± 0.03	2.744AsnLys: 2.744 ± 0.033	4.273AsnLeu: 4.273 ± 0.039	1.058AsnMet: 1.058 ± 0.016	1.948AsnAsn: 1.948 ± 0.023	2.277AsnPro: 2.277 ± 0.028	1.772AsnGln: 1.772 ± 0.027	2.067AsnArg: 2.067 ± 0.027	3.6AsnSer: 3.6 ± 0.04	2.357AsnThr: 2.357 ± 0.025	2.557AsnVal: 2.557 ± 0.028	0.536AsnTrp: 0.536 ± 0.014	1.364AsnTyr: 1.364 ± 0.022	0.001AsnXaa: 0.001 ± 0.0
Pro	3.367ProAla: 3.367 ± 0.039	0.99ProCys: 0.99 ± 0.021	2.579ProAsp: 2.579 ± 0.027	3.788ProGlu: 3.788 ± 0.038	1.94ProPhe: 1.94 ± 0.027	3.49ProGly: 3.49 ± 0.112	1.186ProHis: 1.186 ± 0.02	1.965ProIle: 1.965 ± 0.022	2.77ProLys: 2.77 ± 0.033	4.516ProLeu: 4.516 ± 0.042	0.921ProMet: 0.921 ± 0.017	1.93ProAsn: 1.93 ± 0.026	3.983ProPro: 3.983 ± 0.065	2.258ProGln: 2.258 ± 0.032	2.353ProArg: 2.353 ± 0.03	4.782ProSer: 4.782 ± 0.051	2.589ProThr: 2.589 ± 0.028	3.653ProVal: 3.653 ± 0.034	0.568ProTrp: 0.568 ± 0.013	1.438ProTyr: 1.438 ± 0.021	0.001ProXaa: 0.001 ± 0.0
Gln	2.969GlnAla: 2.969 ± 0.031	0.93GlnCys: 0.93 ± 0.022	2.266GlnAsp: 2.266 ± 0.022	3.726GlnGlu: 3.726 ± 0.04	1.519GlnPhe: 1.519 ± 0.018	2.403GlnGly: 2.403 ± 0.031	1.282GlnHis: 1.282 ± 0.022	2.312GlnIle: 2.312 ± 0.024	3.331GlnLys: 3.331 ± 0.038	4.614GlnLeu: 4.614 ± 0.044	1.129GlnMet: 1.129 ± 0.019	2.116GlnAsn: 2.116 ± 0.027	2.242GlnPro: 2.242 ± 0.03	2.976GlnGln: 2.976 ± 0.057	2.585GlnArg: 2.585 ± 0.03	3.178GlnSer: 3.178 ± 0.035	2.395GlnThr: 2.395 ± 0.028	2.724GlnVal: 2.724 ± 0.026	0.513GlnTrp: 0.513 ± 0.012	1.337GlnTyr: 1.337 ± 0.02	0.001GlnXaa: 0.001 ± 0.0
Arg	2.918ArgAla: 2.918 ± 0.034	1.059ArgCys: 1.059 ± 0.022	2.544ArgAsp: 2.544 ± 0.032	3.706ArgGlu: 3.706 ± 0.038	1.958ArgPhe: 1.958 ± 0.023	2.718ArgGly: 2.718 ± 0.042	1.404ArgHis: 1.404 ± 0.02	2.618ArgIle: 2.618 ± 0.031	3.971ArgLys: 3.971 ± 0.037	4.756ArgLeu: 4.756 ± 0.044	1.162ArgMet: 1.162 ± 0.019	2.268ArgAsn: 2.268 ± 0.027	2.261ArgPro: 2.261 ± 0.03	2.433ArgGln: 2.433 ± 0.03	3.493ArgArg: 3.493 ± 0.046	3.824ArgSer: 3.824 ± 0.055	2.653ArgThr: 2.653 ± 0.03	2.864ArgVal: 2.864 ± 0.028	0.609ArgTrp: 0.609 ± 0.013	1.595ArgTyr: 1.595 ± 0.02	0.001ArgXaa: 0.001 ± 0.001
Ser	4.935SerAla: 4.935 ± 0.046	1.821SerCys: 1.821 ± 0.028	4.044SerAsp: 4.044 ± 0.039	5.184SerGlu: 5.184 ± 0.047	3.208SerPhe: 3.208 ± 0.036	4.82SerGly: 4.82 ± 0.048	2.002SerHis: 2.002 ± 0.022	3.705SerIle: 3.705 ± 0.037	4.675SerLys: 4.675 ± 0.045	8.038SerLeu: 8.038 ± 0.059	1.631SerMet: 1.631 ± 0.024	3.196SerAsn: 3.196 ± 0.035	4.905SerPro: 4.905 ± 0.057	3.646SerGln: 3.646 ± 0.039	3.974SerArg: 3.974 ± 0.051	9.043SerSer: 9.043 ± 0.098	4.574SerThr: 4.574 ± 0.044	5.188SerVal: 5.188 ± 0.047	0.968SerTrp: 0.968 ± 0.016	2.298SerTyr: 2.298 ± 0.028	0.0SerXaa: 0.0 ± 0.0
Thr	3.755ThrAla: 3.755 ± 0.038	1.278ThrCys: 1.278 ± 0.031	2.784ThrAsp: 2.784 ± 0.029	3.852ThrGlu: 3.852 ± 0.037	2.262ThrPhe: 2.262 ± 0.027	3.363ThrGly: 3.363 ± 0.034	1.176ThrHis: 1.176 ± 0.022	2.71ThrIle: 2.71 ± 0.032	3.002ThrLys: 3.002 ± 0.033	5.187ThrLeu: 5.187 ± 0.044	1.176ThrMet: 1.176 ± 0.018	2.013ThrAsn: 2.013 ± 0.025	3.018ThrPro: 3.018 ± 0.042	2.136ThrGln: 2.136 ± 0.025	2.277ThrArg: 2.277 ± 0.026	4.701ThrSer: 4.701 ± 0.048	2.996ThrThr: 2.996 ± 0.037	4.172ThrVal: 4.172 ± 0.038	0.669ThrTrp: 0.669 ± 0.014	1.58ThrTyr: 1.58 ± 0.022	0.001ThrXaa: 0.001 ± 0.0
Val	3.981ValAla: 3.981 ± 0.034	1.553ValCys: 1.553 ± 0.028	3.091ValAsp: 3.091 ± 0.034	3.98ValGlu: 3.98 ± 0.038	2.72ValPhe: 2.72 ± 0.032	3.266ValGly: 3.266 ± 0.03	1.563ValHis: 1.563 ± 0.021	3.412ValIle: 3.412 ± 0.028	4.016ValLys: 4.016 ± 0.037	6.282ValLeu: 6.282 ± 0.051	1.441ValMet: 1.441 ± 0.021	2.671ValAsn: 2.671 ± 0.027	3.377ValPro: 3.377 ± 0.037	2.832ValGln: 2.832 ± 0.027	2.948ValArg: 2.948 ± 0.03	4.974ValSer: 4.974 ± 0.038	3.805ValThr: 3.805 ± 0.038	4.253ValVal: 4.253 ± 0.034	0.723ValTrp: 0.723 ± 0.016	1.92ValTyr: 1.92 ± 0.024	0.001ValXaa: 0.001 ± 0.0
Trp	0.643TrpAla: 0.643 ± 0.014	0.252TrpCys: 0.252 ± 0.008	0.663TrpAsp: 0.663 ± 0.015	0.766TrpGlu: 0.766 ± 0.015	0.461TrpPhe: 0.461 ± 0.013	0.627TrpGly: 0.627 ± 0.018	0.33TrpHis: 0.33 ± 0.01	0.677TrpIle: 0.677 ± 0.013	0.943TrpLys: 0.943 ± 0.02	1.175TrpLeu: 1.175 ± 0.023	0.325TrpMet: 0.325 ± 0.01	0.683TrpAsn: 0.683 ± 0.017	0.429TrpPro: 0.429 ± 0.009	0.537TrpGln: 0.537 ± 0.013	0.62TrpArg: 0.62 ± 0.015	0.85TrpSer: 0.85 ± 0.018	0.65TrpThr: 0.65 ± 0.013	0.667TrpVal: 0.667 ± 0.013	0.201TrpTrp: 0.201 ± 0.008	0.378TrpTyr: 0.378 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.588TyrAla: 1.588 ± 0.023	0.802TyrCys: 0.802 ± 0.016	1.49TyrAsp: 1.49 ± 0.022	1.87TyrGlu: 1.87 ± 0.024	1.493TyrPhe: 1.493 ± 0.025	1.769TyrGly: 1.769 ± 0.024	0.841TyrHis: 0.841 ± 0.015	1.728TyrIle: 1.728 ± 0.021	1.798TyrLys: 1.798 ± 0.024	3.059TyrLeu: 3.059 ± 0.035	0.714TyrMet: 0.714 ± 0.014	1.347TyrAsn: 1.347 ± 0.02	1.357TyrPro: 1.357 ± 0.021	1.33TyrGln: 1.33 ± 0.022	1.688TyrArg: 1.688 ± 0.025	2.457TyrSer: 2.457 ± 0.027	1.678TyrThr: 1.678 ± 0.023	1.782TyrVal: 1.782 ± 0.022	0.394TyrTrp: 0.394 ± 0.01	1.157TyrTyr: 1.157 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.002XaaLeu: 0.002 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.044XaaXaa: 0.044 ± 0.007

Statistics based on 9263 proteins (3777475 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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