Acidianus rod-shaped virus 2
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N9PB33|A0A0N9PB33_9VIRU Uncharacterized protein OS=Acidianus rod-shaped virus 2 OX=1732175 PE=4 SV=1
MM1 pKa = 7.48 NCLTVLKK8 pKa = 10.53 QKK10 pKa = 10.65 GYY12 pKa = 10.6 NLDD15 pKa = 4.13 GYY17 pKa = 11.48 SSDD20 pKa = 3.88 TILALEE26 pKa = 4.18 EE27 pKa = 4.81 AEE29 pKa = 5.12 LDD31 pKa = 3.87 PEE33 pKa = 3.98 EE34 pKa = 5.83 CEE36 pKa = 4.81 ALFHH40 pKa = 6.03 YY41 pKa = 10.78 LEE43 pKa = 4.26 IVYY46 pKa = 10.25 GG47 pKa = 3.84
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.963
IPC2_protein 4.075
IPC_protein 3.872
Toseland 3.732
ProMoST 4.024
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.732
Rodwell 3.719
Grimsley 3.656
Solomon 3.808
Lehninger 3.757
Nozaki 3.986
DTASelect 4.05
Thurlkill 3.77
EMBOSS 3.757
Sillero 3.986
Patrickios 0.121
IPC_peptide 3.808
IPC2_peptide 3.973
IPC2.peptide.svr19 3.917
Protein with the highest isoelectric point:
>tr|A0A0N9PCU3|A0A0N9PCU3_9VIRU Uncharacterized protein OS=Acidianus rod-shaped virus 2 OX=1732175 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.1 GHH5 pKa = 5.63 TPRR8 pKa = 11.84 SFAQRR13 pKa = 11.84 YY14 pKa = 7.09 SKK16 pKa = 10.38 WSAKK20 pKa = 8.15 FTAFSNPTVASTILTNVAPSAQGNFQTNVTKK51 pKa = 9.53 FTSVNEE57 pKa = 3.99 QVSAVLTQYY66 pKa = 11.34 GVTGPMRR73 pKa = 11.84 AIYY76 pKa = 9.6 QGYY79 pKa = 7.76 GLKK82 pKa = 10.09 VARR85 pKa = 11.84 ALNRR89 pKa = 11.84 IGAGPALTNMIAGLKK104 pKa = 9.61 AYY106 pKa = 9.63 YY107 pKa = 10.37 VSAFGANPTILDD119 pKa = 3.62 AVTNIITGSPNGYY132 pKa = 9.96 VSS134 pKa = 3.24
Molecular weight: 14.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.24
IPC2_protein 10.16
IPC_protein 10.467
Toseland 10.233
ProMoST 10.028
Dawson 10.482
Bjellqvist 10.189
Wikipedia 10.687
Rodwell 10.847
Grimsley 10.57
Solomon 10.511
Lehninger 10.467
Nozaki 10.204
DTASelect 10.189
Thurlkill 10.306
EMBOSS 10.643
Sillero 10.379
Patrickios 10.496
IPC_peptide 10.496
IPC2_peptide 8.99
IPC2.peptide.svr19 8.462
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
43
0
43
8767
47
1115
203.9
23.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.692 ± 0.371
0.73 ± 0.177
5.11 ± 0.391
6.593 ± 0.526
5.213 ± 0.263
4.688 ± 0.289
1.152 ± 0.15
9.239 ± 0.421
7.734 ± 0.554
9.581 ± 0.528
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.099 ± 0.173
6.194 ± 0.542
3.901 ± 0.223
4.426 ± 0.38
3.114 ± 0.253
5.794 ± 0.393
5.076 ± 0.255
6.627 ± 0.282
0.913 ± 0.106
6.125 ± 0.319
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here