Xanthomonas phage XAJ2
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I9L2H5|A0A1I9L2H5_9CAUD Uncharacterized protein OS=Xanthomonas phage XAJ2 OX=1775249 PE=4 SV=1
MM1 pKa = 7.38 KK2 pKa = 10.29 RR3 pKa = 11.84 FIIEE7 pKa = 3.98 VVCANASTLQEE18 pKa = 4.26 ALDD21 pKa = 3.93 FASACIGEE29 pKa = 4.7 DD30 pKa = 3.18 PTRR33 pKa = 11.84 YY34 pKa = 9.84 NQMQVDD40 pKa = 4.06 EE41 pKa = 4.64 TDD43 pKa = 3.68 EE44 pKa = 4.01 QLSDD48 pKa = 3.51 PDD50 pKa = 4.31 YY51 pKa = 10.27 IKK53 pKa = 11.11 SSITRR58 pKa = 11.84 NEE60 pKa = 3.49
Molecular weight: 6.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.224
IPC2_protein 4.126
IPC_protein 3.999
Toseland 3.808
ProMoST 4.075
Dawson 3.973
Bjellqvist 4.215
Wikipedia 3.897
Rodwell 3.834
Grimsley 3.732
Solomon 3.948
Lehninger 3.91
Nozaki 4.101
DTASelect 4.266
Thurlkill 3.859
EMBOSS 3.897
Sillero 4.113
Patrickios 3.465
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 4.05
Protein with the highest isoelectric point:
>tr|A0A1I9L2I4|A0A1I9L2I4_9CAUD Uncharacterized protein OS=Xanthomonas phage XAJ2 OX=1775249 PE=4 SV=1
MM1 pKa = 7.65 SYY3 pKa = 10.77 AISSKK8 pKa = 10.91 AKK10 pKa = 8.57 IPKK13 pKa = 9.42 RR14 pKa = 11.84 YY15 pKa = 9.02 RR16 pKa = 11.84 PKK18 pKa = 10.16 RR19 pKa = 11.84 PPVPVVNAMAAYY31 pKa = 8.19 AKK33 pKa = 9.98 QYY35 pKa = 9.76 KK36 pKa = 8.88 RR37 pKa = 11.84 VYY39 pKa = 9.78 GIQPTYY45 pKa = 11.15 VYY47 pKa = 10.73 DD48 pKa = 3.75 HH49 pKa = 6.18 KK50 pKa = 10.01 TKK52 pKa = 10.64 FIRR55 pKa = 11.84 VGEE58 pKa = 4.2 SGGVSLQRR66 pKa = 11.84 IRR68 pKa = 11.84 QLTAQLQLRR77 pKa = 11.84 AGEE80 pKa = 4.19 EE81 pKa = 4.21 SS82 pKa = 3.26
Molecular weight: 9.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.277
IPC2_protein 10.145
IPC_protein 10.657
Toseland 10.555
ProMoST 10.248
Dawson 10.716
Bjellqvist 10.409
Wikipedia 10.906
Rodwell 11.111
Grimsley 10.789
Solomon 10.76
Lehninger 10.73
Nozaki 10.511
DTASelect 10.409
Thurlkill 10.57
EMBOSS 10.95
Sillero 10.628
Patrickios 10.818
IPC_peptide 10.76
IPC2_peptide 9.194
IPC2.peptide.svr19 8.283
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
79
0
79
15513
37
1287
196.4
21.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.411 ± 0.684
0.993 ± 0.166
5.209 ± 0.201
6.311 ± 0.33
3.913 ± 0.161
7.252 ± 0.241
1.508 ± 0.189
5.628 ± 0.178
6.233 ± 0.391
7.909 ± 0.258
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.372 ± 0.175
4.809 ± 0.176
4.39 ± 0.276
4.184 ± 0.398
5.073 ± 0.16
6.466 ± 0.331
5.995 ± 0.365
6.53 ± 0.273
1.612 ± 0.147
3.204 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here