Anaerostipes sp. 494a

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Anaerostipes; unclassified Anaerostipes

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2255 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q9JPX6|A0A1Q9JPX6_9FIRM Glycerol-3-phosphate acyltransferase OS=Anaerostipes sp. 494a OX=1261636 GN=plsY PE=3 SV=1
MM1 pKa = 7.83DD2 pKa = 5.55RR3 pKa = 11.84EE4 pKa = 4.4QMICPCLDD12 pKa = 3.16VTAGQIMDD20 pKa = 4.32AYY22 pKa = 9.88EE23 pKa = 4.27EE24 pKa = 4.61GATTVDD30 pKa = 3.97AIKK33 pKa = 10.56DD34 pKa = 3.34ITGAGSVCGACIDD47 pKa = 4.03QIEE50 pKa = 4.55DD51 pKa = 3.93LLIEE55 pKa = 4.32LKK57 pKa = 10.92GG58 pKa = 3.46

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q9JTT9|A0A1Q9JTT9_9FIRM 5-formyltetrahydrofolate cyclo-ligase OS=Anaerostipes sp. 494a OX=1261636 GN=BHF70_07935 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.25VLKK32 pKa = 10.25SRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84NRR41 pKa = 11.84LSAA44 pKa = 3.84

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2255

0

2255

705304

37

2115

312.8

35.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.668 ± 0.053

1.431 ± 0.021

5.662 ± 0.045

7.623 ± 0.06

4.179 ± 0.038

7.069 ± 0.051

1.851 ± 0.024

8.196 ± 0.051

8.086 ± 0.058

8.572 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.232 ± 0.025

4.491 ± 0.032

3.184 ± 0.026

3.342 ± 0.028

3.941 ± 0.039

5.587 ± 0.045

5.198 ± 0.042

6.778 ± 0.045

0.771 ± 0.015

4.137 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski