Amino acid dipepetide frequency for Anaerostipes sp. 494a

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.613AlaAla: 5.613 ± 0.138
0.964AlaCys: 0.964 ± 0.042
3.796AlaAsp: 3.796 ± 0.072
4.228AlaGlu: 4.228 ± 0.085
2.838AlaPhe: 2.838 ± 0.069
5.415AlaGly: 5.415 ± 0.106
1.036AlaHis: 1.036 ± 0.043
5.15AlaIle: 5.15 ± 0.118
5.355AlaLys: 5.355 ± 0.105
5.996AlaLeu: 5.996 ± 0.097
2.286AlaMet: 2.286 ± 0.053
2.432AlaAsn: 2.432 ± 0.069
1.721AlaPro: 1.721 ± 0.054
2.043AlaGln: 2.043 ± 0.064
2.556AlaArg: 2.556 ± 0.062
3.515AlaSer: 3.515 ± 0.071
3.148AlaThr: 3.148 ± 0.081
5.569AlaVal: 5.569 ± 0.096
0.421AlaTrp: 0.421 ± 0.025
2.603AlaTyr: 2.603 ± 0.064
0.0AlaXaa: 0.0 ± 0.0
Cys
0.885CysAla: 0.885 ± 0.033
0.255CysCys: 0.255 ± 0.019
0.798CysAsp: 0.798 ± 0.039
0.862CysGlu: 0.862 ± 0.03
0.566CysPhe: 0.566 ± 0.028
1.438CysGly: 1.438 ± 0.05
0.32CysHis: 0.32 ± 0.024
1.216CysIle: 1.216 ± 0.045
0.924CysLys: 0.924 ± 0.039
1.136CysLeu: 1.136 ± 0.045
0.428CysMet: 0.428 ± 0.027
0.669CysAsn: 0.669 ± 0.028
0.631CysPro: 0.631 ± 0.038
0.432CysGln: 0.432 ± 0.024
0.566CysArg: 0.566 ± 0.03
0.863CysSer: 0.863 ± 0.034
0.699CysThr: 0.699 ± 0.032
0.953CysVal: 0.953 ± 0.036
0.115CysTrp: 0.115 ± 0.013
0.554CysTyr: 0.554 ± 0.03
0.0CysXaa: 0.0 ± 0.0
Asp
3.727AspAla: 3.727 ± 0.09
0.777AspCys: 0.777 ± 0.034
3.072AspAsp: 3.072 ± 0.073
4.479AspGlu: 4.479 ± 0.08
2.785AspPhe: 2.785 ± 0.07
4.167AspGly: 4.167 ± 0.098
1.139AspHis: 1.139 ± 0.04
4.955AspIle: 4.955 ± 0.077
3.912AspLys: 3.912 ± 0.083
4.685AspLeu: 4.685 ± 0.091
1.951AspMet: 1.951 ± 0.055
2.154AspAsn: 2.154 ± 0.054
1.741AspPro: 1.741 ± 0.053
1.918AspGln: 1.918 ± 0.063
2.086AspArg: 2.086 ± 0.056
3.024AspSer: 3.024 ± 0.073
2.864AspThr: 2.864 ± 0.061
3.841AspVal: 3.841 ± 0.08
0.464AspTrp: 0.464 ± 0.024
2.875AspTyr: 2.875 ± 0.065
0.0AspXaa: 0.0 ± 0.0
Glu
4.869GluAla: 4.869 ± 0.087
0.851GluCys: 0.851 ± 0.038
4.517GluAsp: 4.517 ± 0.089
7.522GluGlu: 7.522 ± 0.133
2.722GluPhe: 2.722 ± 0.062
4.302GluGly: 4.302 ± 0.083
1.367GluHis: 1.367 ± 0.041
6.591GluIle: 6.591 ± 0.113
7.597GluLys: 7.597 ± 0.121
6.234GluLeu: 6.234 ± 0.124
2.573GluMet: 2.573 ± 0.061
4.255GluAsn: 4.255 ± 0.086
1.768GluPro: 1.768 ± 0.06
2.792GluGln: 2.792 ± 0.062
3.048GluArg: 3.048 ± 0.067
3.272GluSer: 3.272 ± 0.063
3.805GluThr: 3.805 ± 0.074
4.554GluVal: 4.554 ± 0.083
0.509GluTrp: 0.509 ± 0.03
3.082GluTyr: 3.082 ± 0.069
0.0GluXaa: 0.0 ± 0.0
Phe
2.685PheAla: 2.685 ± 0.061
0.754PheCys: 0.754 ± 0.03
2.568PheAsp: 2.568 ± 0.059
2.78PheGlu: 2.78 ± 0.063
2.03PhePhe: 2.03 ± 0.064
3.194PheGly: 3.194 ± 0.081
0.998PheHis: 0.998 ± 0.041
3.201PheIle: 3.201 ± 0.078
2.364PheLys: 2.364 ± 0.066
4.031PheLeu: 4.031 ± 0.093
1.285PheMet: 1.285 ± 0.042
1.501PheAsn: 1.501 ± 0.046
1.446PhePro: 1.446 ± 0.051
1.578PheGln: 1.578 ± 0.046
1.475PheArg: 1.475 ± 0.054
2.763PheSer: 2.763 ± 0.073
2.141PheThr: 2.141 ± 0.053
2.882PheVal: 2.882 ± 0.065
0.359PheTrp: 0.359 ± 0.024
1.758PheTyr: 1.758 ± 0.051
0.0PheXaa: 0.0 ± 0.0
Gly
4.801GlyAla: 4.801 ± 0.097
1.317GlyCys: 1.317 ± 0.05
3.516GlyAsp: 3.516 ± 0.073
4.421GlyGlu: 4.421 ± 0.088
3.213GlyPhe: 3.213 ± 0.075
4.638GlyGly: 4.638 ± 0.105
1.313GlyHis: 1.313 ± 0.046
6.764GlyIle: 6.764 ± 0.116
6.346GlyLys: 6.346 ± 0.096
5.691GlyLeu: 5.691 ± 0.109
2.405GlyMet: 2.405 ± 0.061
3.359GlyAsn: 3.359 ± 0.073
1.402GlyPro: 1.402 ± 0.051
2.125GlyGln: 2.125 ± 0.059
2.749GlyArg: 2.749 ± 0.062
3.737GlySer: 3.737 ± 0.063
4.146GlyThr: 4.146 ± 0.088
4.934GlyVal: 4.934 ± 0.1
0.563GlyTrp: 0.563 ± 0.032
3.248GlyTyr: 3.248 ± 0.08
0.0GlyXaa: 0.0 ± 0.0
His
1.082HisAla: 1.082 ± 0.034
0.328HisCys: 0.328 ± 0.023
0.899HisAsp: 0.899 ± 0.043
1.055HisGlu: 1.055 ± 0.038
0.872HisPhe: 0.872 ± 0.04
1.523HisGly: 1.523 ± 0.048
0.486HisHis: 0.486 ± 0.036
1.47HisIle: 1.47 ± 0.038
1.251HisLys: 1.251 ± 0.045
1.683HisLeu: 1.683 ± 0.053
0.624HisMet: 0.624 ± 0.031
0.824HisAsn: 0.824 ± 0.033
0.926HisPro: 0.926 ± 0.039
0.658HisGln: 0.658 ± 0.03
0.749HisArg: 0.749 ± 0.032
0.977HisSer: 0.977 ± 0.041
0.906HisThr: 0.906 ± 0.038
1.178HisVal: 1.178 ± 0.039
0.203HisTrp: 0.203 ± 0.017
0.822HisTyr: 0.822 ± 0.035
0.0HisXaa: 0.0 ± 0.0
Ile
5.776IleAla: 5.776 ± 0.1
1.452IleCys: 1.452 ± 0.046
4.521IleAsp: 4.521 ± 0.077
5.65IleGlu: 5.65 ± 0.097
3.45IlePhe: 3.45 ± 0.079
5.653IleGly: 5.653 ± 0.121
1.703IleHis: 1.703 ± 0.054
6.407IleIle: 6.407 ± 0.109
5.51IleLys: 5.51 ± 0.092
7.612IleLeu: 7.612 ± 0.113
2.3IleMet: 2.3 ± 0.066
3.19IleAsn: 3.19 ± 0.065
3.441IlePro: 3.441 ± 0.078
2.804IleGln: 2.804 ± 0.066
3.536IleArg: 3.536 ± 0.071
5.429IleSer: 5.429 ± 0.092
4.49IleThr: 4.49 ± 0.092
5.321IleVal: 5.321 ± 0.099
0.588IleTrp: 0.588 ± 0.031
3.129IleTyr: 3.129 ± 0.061
0.0IleXaa: 0.0 ± 0.0
Lys
5.287LysAla: 5.287 ± 0.093
0.725LysCys: 0.725 ± 0.035
5.05LysAsp: 5.05 ± 0.089
8.545LysGlu: 8.545 ± 0.13
2.257LysPhe: 2.257 ± 0.055
4.853LysGly: 4.853 ± 0.083
1.246LysHis: 1.246 ± 0.04
6.61LysIle: 6.61 ± 0.103
8.148LysLys: 8.148 ± 0.143
5.786LysLeu: 5.786 ± 0.094
2.61LysMet: 2.61 ± 0.059
4.395LysAsn: 4.395 ± 0.078
2.117LysPro: 2.117 ± 0.048
2.884LysGln: 2.884 ± 0.065
3.281LysArg: 3.281 ± 0.073
3.743LysSer: 3.743 ± 0.095
4.133LysThr: 4.133 ± 0.082
5.235LysVal: 5.235 ± 0.103
0.549LysTrp: 0.549 ± 0.028
3.403LysTyr: 3.403 ± 0.066
0.0LysXaa: 0.0 ± 0.0
Leu
5.576LeuAla: 5.576 ± 0.1
1.33LeuCys: 1.33 ± 0.044
4.988LeuAsp: 4.988 ± 0.093
6.42LeuGlu: 6.42 ± 0.116
3.553LeuPhe: 3.553 ± 0.086
5.966LeuGly: 5.966 ± 0.12
1.443LeuHis: 1.443 ± 0.045
6.451LeuIle: 6.451 ± 0.103
7.12LeuLys: 7.12 ± 0.115
7.592LeuLeu: 7.592 ± 0.133
2.697LeuMet: 2.697 ± 0.061
4.058LeuAsn: 4.058 ± 0.075
3.19LeuPro: 3.19 ± 0.07
2.65LeuGln: 2.65 ± 0.067
3.287LeuArg: 3.287 ± 0.074
5.762LeuSer: 5.762 ± 0.096
4.609LeuThr: 4.609 ± 0.087
5.188LeuVal: 5.188 ± 0.096
0.706LeuTrp: 0.706 ± 0.035
3.131LeuTyr: 3.131 ± 0.071
0.0LeuXaa: 0.0 ± 0.0
Met
2.382MetAla: 2.382 ± 0.063
0.32MetCys: 0.32 ± 0.018
2.104MetAsp: 2.104 ± 0.056
2.932MetGlu: 2.932 ± 0.064
1.175MetPhe: 1.175 ± 0.042
2.152MetGly: 2.152 ± 0.058
0.442MetHis: 0.442 ± 0.028
2.653MetIle: 2.653 ± 0.069
3.184MetLys: 3.184 ± 0.071
2.536MetLeu: 2.536 ± 0.056
1.14MetMet: 1.14 ± 0.044
1.677MetAsn: 1.677 ± 0.051
1.045MetPro: 1.045 ± 0.036
0.924MetGln: 0.924 ± 0.036
1.052MetArg: 1.052 ± 0.039
1.696MetSer: 1.696 ± 0.044
1.782MetThr: 1.782 ± 0.051
2.002MetVal: 2.002 ± 0.052
0.186MetTrp: 0.186 ± 0.017
0.937MetTyr: 0.937 ± 0.04
0.0MetXaa: 0.0 ± 0.0
Asn
2.802AsnAla: 2.802 ± 0.06
0.611AsnCys: 0.611 ± 0.029
2.266AsnAsp: 2.266 ± 0.06
2.922AsnGlu: 2.922 ± 0.067
1.684AsnPhe: 1.684 ± 0.048
3.501AsnGly: 3.501 ± 0.074
1.131AsnHis: 1.131 ± 0.044
4.0AsnIle: 4.0 ± 0.07
3.227AsnLys: 3.227 ± 0.071
3.844AsnLeu: 3.844 ± 0.078
1.452AsnMet: 1.452 ± 0.043
1.786AsnAsn: 1.786 ± 0.055
2.037AsnPro: 2.037 ± 0.06
2.029AsnGln: 2.029 ± 0.064
1.986AsnArg: 1.986 ± 0.051
2.338AsnSer: 2.338 ± 0.066
2.176AsnThr: 2.176 ± 0.062
2.945AsnVal: 2.945 ± 0.07
0.325AsnTrp: 0.325 ± 0.023
1.843AsnTyr: 1.843 ± 0.053
0.0AsnXaa: 0.0 ± 0.0
Pro
2.115ProAla: 2.115 ± 0.063
0.466ProCys: 0.466 ± 0.027
2.094ProAsp: 2.094 ± 0.063
3.278ProGlu: 3.278 ± 0.074
1.513ProPhe: 1.513 ± 0.047
2.379ProGly: 2.379 ± 0.055
0.567ProHis: 0.567 ± 0.029
2.189ProIle: 2.189 ± 0.058
2.233ProLys: 2.233 ± 0.061
2.597ProLeu: 2.597 ± 0.073
0.981ProMet: 0.981 ± 0.039
1.259ProAsn: 1.259 ± 0.043
0.638ProPro: 0.638 ± 0.033
0.916ProGln: 0.916 ± 0.034
0.964ProArg: 0.964 ± 0.041
1.657ProSer: 1.657 ± 0.059
1.433ProThr: 1.433 ± 0.043
2.797ProVal: 2.797 ± 0.061
0.309ProTrp: 0.309 ± 0.022
1.456ProTyr: 1.456 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
2.105GlnAla: 2.105 ± 0.06
0.4GlnCys: 0.4 ± 0.027
1.553GlnAsp: 1.553 ± 0.049
2.592GlnGlu: 2.592 ± 0.059
1.194GlnPhe: 1.194 ± 0.043
2.219GlnGly: 2.219 ± 0.053
0.468GlnHis: 0.468 ± 0.028
2.999GlnIle: 2.999 ± 0.071
3.298GlnLys: 3.298 ± 0.067
2.96GlnLeu: 2.96 ± 0.064
1.317GlnMet: 1.317 ± 0.046
1.604GlnAsn: 1.604 ± 0.051
0.89GlnPro: 0.89 ± 0.035
1.201GlnGln: 1.201 ± 0.047
1.414GlnArg: 1.414 ± 0.044
1.523GlnSer: 1.523 ± 0.045
1.669GlnThr: 1.669 ± 0.055
2.182GlnVal: 2.182 ± 0.054
0.384GlnTrp: 0.384 ± 0.026
1.448GlnTyr: 1.448 ± 0.05
0.0GlnXaa: 0.0 ± 0.0
Arg
2.148ArgAla: 2.148 ± 0.059
0.518ArgCys: 0.518 ± 0.031
2.012ArgAsp: 2.012 ± 0.063
3.308ArgGlu: 3.308 ± 0.077
1.716ArgPhe: 1.716 ± 0.043
2.264ArgGly: 2.264 ± 0.053
0.76ArgHis: 0.76 ± 0.035
3.404ArgIle: 3.404 ± 0.071
3.762ArgLys: 3.762 ± 0.071
3.399ArgLeu: 3.399 ± 0.075
1.351ArgMet: 1.351 ± 0.044
2.111ArgAsn: 2.111 ± 0.057
1.188ArgPro: 1.188 ± 0.041
1.509ArgGln: 1.509 ± 0.047
1.822ArgArg: 1.822 ± 0.054
1.881ArgSer: 1.881 ± 0.053
1.985ArgThr: 1.985 ± 0.049
2.301ArgVal: 2.301 ± 0.068
0.292ArgTrp: 0.292 ± 0.022
1.68ArgTyr: 1.68 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
3.553SerAla: 3.553 ± 0.083
0.742SerCys: 0.742 ± 0.032
2.994SerAsp: 2.994 ± 0.067
3.525SerGlu: 3.525 ± 0.083
2.725SerPhe: 2.725 ± 0.068
4.655SerGly: 4.655 ± 0.084
0.991SerHis: 0.991 ± 0.041
4.435SerIle: 4.435 ± 0.095
3.949SerLys: 3.949 ± 0.087
4.92SerLeu: 4.92 ± 0.087
1.933SerMet: 1.933 ± 0.058
2.39SerAsn: 2.39 ± 0.066
1.667SerPro: 1.667 ± 0.052
1.738SerGln: 1.738 ± 0.058
2.392SerArg: 2.392 ± 0.055
3.546SerSer: 3.546 ± 0.095
2.701SerThr: 2.701 ± 0.073
4.119SerVal: 4.119 ± 0.087
0.466SerTrp: 0.466 ± 0.028
2.433SerTyr: 2.433 ± 0.075
0.0SerXaa: 0.0 ± 0.0
Thr
3.798ThrAla: 3.798 ± 0.091
0.669ThrCys: 0.669 ± 0.029
2.916ThrAsp: 2.916 ± 0.079
3.399ThrGlu: 3.399 ± 0.082
2.166ThrPhe: 2.166 ± 0.053
4.543ThrGly: 4.543 ± 0.092
0.812ThrHis: 0.812 ± 0.035
4.188ThrIle: 4.188 ± 0.078
3.811ThrLys: 3.811 ± 0.08
4.476ThrLeu: 4.476 ± 0.072
1.541ThrMet: 1.541 ± 0.044
2.066ThrAsn: 2.066 ± 0.054
1.921ThrPro: 1.921 ± 0.052
1.392ThrGln: 1.392 ± 0.047
1.802ThrArg: 1.802 ± 0.05
2.976ThrSer: 2.976 ± 0.076
2.856ThrThr: 2.856 ± 0.081
4.124ThrVal: 4.124 ± 0.08
0.405ThrTrp: 0.405 ± 0.022
2.12ThrTyr: 2.12 ± 0.067
0.0ThrXaa: 0.0 ± 0.0
Val
4.639ValAla: 4.639 ± 0.102
1.093ValCys: 1.093 ± 0.043
3.939ValAsp: 3.939 ± 0.082
4.685ValGlu: 4.685 ± 0.079
3.07ValPhe: 3.07 ± 0.066
4.221ValGly: 4.221 ± 0.083
1.165ValHis: 1.165 ± 0.042
5.558ValIle: 5.558 ± 0.101
5.28ValLys: 5.28 ± 0.095
6.185ValLeu: 6.185 ± 0.091
2.046ValMet: 2.046 ± 0.063
2.899ValAsn: 2.899 ± 0.073
2.47ValPro: 2.47 ± 0.058
1.818ValGln: 1.818 ± 0.042
2.466ValArg: 2.466 ± 0.07
4.448ValSer: 4.448 ± 0.081
4.061ValThr: 4.061 ± 0.086
4.699ValVal: 4.699 ± 0.091
0.483ValTrp: 0.483 ± 0.023
2.556ValTyr: 2.556 ± 0.064
0.0ValXaa: 0.0 ± 0.0
Trp
0.418TrpAla: 0.418 ± 0.025
0.111TrpCys: 0.111 ± 0.012
0.423TrpAsp: 0.423 ± 0.022
0.573TrpGlu: 0.573 ± 0.027
0.343TrpPhe: 0.343 ± 0.022
0.549TrpGly: 0.549 ± 0.031
0.163TrpHis: 0.163 ± 0.017
0.645TrpIle: 0.645 ± 0.038
0.759TrpLys: 0.759 ± 0.035
0.666TrpLeu: 0.666 ± 0.031
0.302TrpMet: 0.302 ± 0.018
0.493TrpAsn: 0.493 ± 0.027
0.194TrpPro: 0.194 ± 0.019
0.224TrpGln: 0.224 ± 0.018
0.318TrpArg: 0.318 ± 0.023
0.411TrpSer: 0.411 ± 0.027
0.322TrpThr: 0.322 ± 0.023
0.401TrpVal: 0.401 ± 0.025
0.074TrpTrp: 0.074 ± 0.01
0.325TrpTyr: 0.325 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.422TyrAla: 2.422 ± 0.07
0.63TyrCys: 0.63 ± 0.033
2.589TyrAsp: 2.589 ± 0.075
3.058TyrGlu: 3.058 ± 0.068
1.923TyrPhe: 1.923 ± 0.057
3.058TyrGly: 3.058 ± 0.079
0.961TyrHis: 0.961 ± 0.039
3.075TyrIle: 3.075 ± 0.068
2.829TyrLys: 2.829 ± 0.066
3.688TyrLeu: 3.688 ± 0.095
1.102TyrMet: 1.102 ± 0.041
1.782TyrAsn: 1.782 ± 0.055
1.409TyrPro: 1.409 ± 0.049
1.782TyrGln: 1.782 ± 0.049
1.863TyrArg: 1.863 ± 0.044
2.311TyrSer: 2.311 ± 0.056
2.057TyrThr: 2.057 ± 0.065
2.554TyrVal: 2.554 ± 0.054
0.312TyrTrp: 0.312 ± 0.022
1.965TyrTyr: 1.965 ± 0.064
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2255 proteins (705304 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski