Streptococcus phage Javan1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A201|A0A4D6A201_9CAUD Uncharacterized protein OS=Streptococcus phage Javan1 OX=2547971 GN=Javan1_0022 PE=4 SV=1
MM1 pKa = 6.96TQDD4 pKa = 3.62EE5 pKa = 5.71LIEE8 pKa = 4.07MMNSDD13 pKa = 3.6VAIGIKK19 pKa = 9.39EE20 pKa = 4.24WIQLRR25 pKa = 11.84DD26 pKa = 3.63HH27 pKa = 6.6YY28 pKa = 9.64LQCIMSFEE36 pKa = 3.79MGYY39 pKa = 9.49RR40 pKa = 11.84LSYY43 pKa = 7.67ITEE46 pKa = 4.0YY47 pKa = 11.43VKK49 pKa = 10.7FLEE52 pKa = 4.19DD53 pKa = 4.83HH54 pKa = 6.49FMEE57 pKa = 5.48DD58 pKa = 3.41LL59 pKa = 4.13

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZX83|A0A4D5ZX83_9CAUD Uncharacterized protein OS=Streptococcus phage Javan1 OX=2547971 GN=Javan1_0041 PE=4 SV=1
MM1 pKa = 6.15TTHH4 pKa = 6.25MKK6 pKa = 9.47SAVMFVAILLLTTLFLMVSAIQRR29 pKa = 11.84LQWQIDD35 pKa = 3.6GLRR38 pKa = 11.84QRR40 pKa = 11.84NEE42 pKa = 4.06VITQQLQEE50 pKa = 4.01LNRR53 pKa = 11.84YY54 pKa = 8.12VGYY57 pKa = 9.78PGGG60 pKa = 3.67

Molecular weight: 6.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

10574

42

1023

207.3

23.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.686 ± 0.483

0.492 ± 0.097

6.119 ± 0.2

8.445 ± 0.49

4.029 ± 0.211

6.081 ± 0.502

1.664 ± 0.191

7.197 ± 0.264

7.878 ± 0.368

8.682 ± 0.338

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.241 ± 0.134

5.249 ± 0.258

2.78 ± 0.246

3.802 ± 0.228

4.899 ± 0.302

6.525 ± 0.291

5.731 ± 0.258

6.346 ± 0.288

0.955 ± 0.125

4.199 ± 0.368
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski