Capybara microvirus Cap1_SP_162
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W816|A0A4P8W816_9VIRU Nonstructural protein OS=Capybara microvirus Cap1_SP_162 OX=2585398 PE=4 SV=1
MM1 pKa = 7.62 AVYY4 pKa = 10.35 KK5 pKa = 10.07 IYY7 pKa = 10.76 SVQDD11 pKa = 3.14 VLIGFNAPFIMINDD25 pKa = 5.17 DD26 pKa = 3.11 IAKK29 pKa = 9.93 RR30 pKa = 11.84 EE31 pKa = 4.0 YY32 pKa = 11.1 RR33 pKa = 11.84 NMLEE37 pKa = 4.31 TNPNRR42 pKa = 11.84 ADD44 pKa = 3.14 MRR46 pKa = 11.84 LFRR49 pKa = 11.84 LASFDD54 pKa = 4.83 DD55 pKa = 3.89 EE56 pKa = 4.74 TGMVTPEE63 pKa = 3.64 ITPVLVEE70 pKa = 4.37 GGANGSNKK78 pKa = 10.29 DD79 pKa = 3.65 SVV81 pKa = 3.41
Molecular weight: 9.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.648
IPC2_protein 4.596
IPC_protein 4.431
Toseland 4.24
ProMoST 4.558
Dawson 4.393
Bjellqvist 4.546
Wikipedia 4.304
Rodwell 4.253
Grimsley 4.164
Solomon 4.38
Lehninger 4.342
Nozaki 4.507
DTASelect 4.711
Thurlkill 4.279
EMBOSS 4.317
Sillero 4.533
Patrickios 4.139
IPC_peptide 4.38
IPC2_peptide 4.52
IPC2.peptide.svr19 4.497
Protein with the highest isoelectric point:
>tr|A0A4P8W5B1|A0A4P8W5B1_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_162 OX=2585398 PE=4 SV=1
MM1 pKa = 7.61 ARR3 pKa = 11.84 LEE5 pKa = 4.86 LPGQTYY11 pKa = 10.52 INKK14 pKa = 9.45 QGKK17 pKa = 9.05 KK18 pKa = 6.88 SHH20 pKa = 6.27 RR21 pKa = 11.84 CEE23 pKa = 3.58 ITSWADD29 pKa = 3.08 VEE31 pKa = 4.41 QNPKK35 pKa = 9.99 FYY37 pKa = 8.79 KK38 pKa = 9.22 TKK40 pKa = 8.64 YY41 pKa = 9.32 HH42 pKa = 6.59 RR43 pKa = 11.84 VDD45 pKa = 4.88 KK46 pKa = 10.55 IPCGQCMEE54 pKa = 4.33 CRR56 pKa = 11.84 LNKK59 pKa = 9.48 ARR61 pKa = 11.84 QWANRR66 pKa = 11.84 IVLEE70 pKa = 4.17 KK71 pKa = 10.13 QDD73 pKa = 3.83 YY74 pKa = 10.46 PEE76 pKa = 4.05 EE77 pKa = 4.16 QVWFLTLTYY86 pKa = 10.57 DD87 pKa = 4.56 DD88 pKa = 3.88 EE89 pKa = 4.66 HH90 pKa = 8.8 VPFGMTVDD98 pKa = 3.63 MEE100 pKa = 4.49 TGEE103 pKa = 4.7 EE104 pKa = 4.08 IVNMTLKK111 pKa = 10.85 KK112 pKa = 9.32 EE113 pKa = 4.14 DD114 pKa = 3.8 EE115 pKa = 4.53 VKK117 pKa = 10.69 FMHH120 pKa = 7.51 DD121 pKa = 2.84 LRR123 pKa = 11.84 QYY125 pKa = 8.02 YY126 pKa = 7.61 QRR128 pKa = 11.84 KK129 pKa = 8.84 YY130 pKa = 10.24 NIDD133 pKa = 3.75 GIRR136 pKa = 11.84 FYY138 pKa = 10.73 MAGEE142 pKa = 4.16 YY143 pKa = 10.88 GSTTNRR149 pKa = 11.84 PHH151 pKa = 5.34 YY152 pKa = 10.46 HH153 pKa = 6.18 YY154 pKa = 10.43 ILYY157 pKa = 10.07 GLPLDD162 pKa = 3.6 QTKK165 pKa = 10.7 LKK167 pKa = 10.28 RR168 pKa = 11.84 ISINEE173 pKa = 4.02 LGQTLWTHH181 pKa = 5.92 EE182 pKa = 4.65 EE183 pKa = 4.15 IEE185 pKa = 4.51 KK186 pKa = 10.03 IWNKK190 pKa = 10.35 GMITIGRR197 pKa = 11.84 VTWEE201 pKa = 4.03 SACYY205 pKa = 8.4 VARR208 pKa = 11.84 YY209 pKa = 6.18 CTKK212 pKa = 10.74 KK213 pKa = 9.16 ITGKK217 pKa = 10.29 DD218 pKa = 3.29 AEE220 pKa = 4.61 WYY222 pKa = 9.94 YY223 pKa = 10.76 KK224 pKa = 10.35 AQGIIPPFTQMSRR237 pKa = 11.84 RR238 pKa = 11.84 PGIGANYY245 pKa = 10.14 LEE247 pKa = 4.43 KK248 pKa = 11.07 NKK250 pKa = 10.57 DD251 pKa = 3.47 AIYY254 pKa = 8.01 TTDD257 pKa = 4.55 SIPIANKK264 pKa = 8.51 KK265 pKa = 6.57 TASLVSPPKK274 pKa = 10.25 SFDD277 pKa = 3.85 RR278 pKa = 11.84 IMEE281 pKa = 4.25 KK282 pKa = 10.82 YY283 pKa = 9.43 EE284 pKa = 3.9 PEE286 pKa = 3.82 FMEE289 pKa = 3.75 QLKK292 pKa = 10.26 RR293 pKa = 11.84 EE294 pKa = 3.94 RR295 pKa = 11.84 RR296 pKa = 11.84 RR297 pKa = 11.84 RR298 pKa = 11.84 AEE300 pKa = 3.86 LNEE303 pKa = 3.7 AAKK306 pKa = 10.59 FKK308 pKa = 10.59 ATDD311 pKa = 3.59 LTPTEE316 pKa = 3.34 WRR318 pKa = 11.84 YY319 pKa = 10.26 QNEE322 pKa = 4.26 EE323 pKa = 4.08 KK324 pKa = 10.55 IKK326 pKa = 10.89 ARR328 pKa = 11.84 TKK330 pKa = 10.33 SLVRR334 pKa = 11.84 EE335 pKa = 4.31 LL336 pKa = 4.01
Molecular weight: 39.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.426
IPC2_protein 8.39
IPC_protein 8.287
Toseland 8.799
ProMoST 8.77
Dawson 9.18
Bjellqvist 9.077
Wikipedia 9.341
Rodwell 9.326
Grimsley 9.194
Solomon 9.238
Lehninger 9.194
Nozaki 9.136
DTASelect 8.975
Thurlkill 9.063
EMBOSS 9.311
Sillero 9.238
Patrickios 4.457
IPC_peptide 9.238
IPC2_peptide 7.746
IPC2.peptide.svr19 7.671
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1444
75
536
240.7
27.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.28 ± 2.379
0.9 ± 0.291
4.848 ± 0.614
8.102 ± 0.992
3.116 ± 0.466
6.371 ± 0.625
1.385 ± 0.339
6.648 ± 0.559
6.717 ± 1.2
6.025 ± 0.421
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.047 ± 0.311
4.848 ± 0.45
4.155 ± 0.84
4.432 ± 0.379
5.263 ± 0.834
7.271 ± 2.116
6.925 ± 0.764
3.74 ± 0.562
1.87 ± 0.314
5.055 ± 0.576
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here