Mycobacterium phage Nitzel

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6T5A1|A0A5J6T5A1_9CAUD Uncharacterized protein OS=Mycobacterium phage Nitzel OX=2652404 GN=82 PE=4 SV=1
MM1 pKa = 7.51SHH3 pKa = 6.63EE4 pKa = 4.44PDD6 pKa = 3.71EE7 pKa = 4.75IEE9 pKa = 5.63PISQMEE15 pKa = 4.2LEE17 pKa = 4.32ILANSGDD24 pKa = 4.27LEE26 pKa = 4.46LATQVGEE33 pKa = 3.85IARR36 pKa = 11.84WAFTQTVEE44 pKa = 4.48SLCTLLCLPPEE55 pKa = 4.38VAEE58 pKa = 4.0QMVNEE63 pKa = 4.22WFLRR67 pKa = 3.83

Molecular weight: 7.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6T3C0|A0A5J6T3C0_9CAUD Uncharacterized protein OS=Mycobacterium phage Nitzel OX=2652404 GN=48 PE=4 SV=1
MM1 pKa = 7.45SRR3 pKa = 11.84PNGQIILTSEE13 pKa = 3.9MYY15 pKa = 10.33NRR17 pKa = 11.84RR18 pKa = 11.84SDD20 pKa = 3.4AKK22 pKa = 9.44RR23 pKa = 11.84AARR26 pKa = 11.84MFIRR30 pKa = 11.84EE31 pKa = 4.26VFSSIVFRR39 pKa = 11.84YY40 pKa = 9.03YY41 pKa = 10.68NRR43 pKa = 11.84DD44 pKa = 3.14GEE46 pKa = 4.34RR47 pKa = 11.84VQEE50 pKa = 4.72NIRR53 pKa = 11.84DD54 pKa = 3.64WLRR57 pKa = 11.84RR58 pKa = 3.57

Molecular weight: 7.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

17518

30

1213

178.8

19.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.566 ± 0.493

1.233 ± 0.171

6.616 ± 0.238

5.982 ± 0.399

2.968 ± 0.191

8.854 ± 0.642

2.169 ± 0.165

4.515 ± 0.182

3.362 ± 0.187

7.227 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.283 ± 0.131

3.425 ± 0.18

6.011 ± 0.179

3.494 ± 0.216

6.713 ± 0.413

5.834 ± 0.304

6.513 ± 0.27

7.438 ± 0.272

2.352 ± 0.145

2.443 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski