Amino acid dipepetide frequency for Mycobacterium phage Nitzel

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.5AlaAla: 14.5 ± 1.606
0.742AlaCys: 0.742 ± 0.216
7.136AlaAsp: 7.136 ± 0.596
7.364AlaGlu: 7.364 ± 0.708
3.14AlaPhe: 3.14 ± 0.369
9.819AlaGly: 9.819 ± 1.386
2.226AlaHis: 2.226 ± 0.381
4.567AlaIle: 4.567 ± 0.595
4.681AlaLys: 4.681 ± 0.553
7.593AlaLeu: 7.593 ± 0.776
2.341AlaMet: 2.341 ± 0.432
2.398AlaAsn: 2.398 ± 0.336
4.91AlaPro: 4.91 ± 0.585
3.597AlaGln: 3.597 ± 0.489
8.049AlaArg: 8.049 ± 0.865
5.309AlaSer: 5.309 ± 0.51
5.766AlaThr: 5.766 ± 0.482
6.965AlaVal: 6.965 ± 0.653
2.112AlaTrp: 2.112 ± 0.354
2.455AlaTyr: 2.455 ± 0.38
0.0AlaXaa: 0.0 ± 0.0
Cys
0.856CysAla: 0.856 ± 0.253
0.0CysCys: 0.0 ± 0.0
1.199CysAsp: 1.199 ± 0.31
0.97CysGlu: 0.97 ± 0.227
0.285CysPhe: 0.285 ± 0.124
1.77CysGly: 1.77 ± 0.302
0.114CysHis: 0.114 ± 0.085
0.228CysIle: 0.228 ± 0.111
0.514CysLys: 0.514 ± 0.203
0.97CysLeu: 0.97 ± 0.236
0.114CysMet: 0.114 ± 0.076
0.514CysAsn: 0.514 ± 0.18
1.085CysPro: 1.085 ± 0.246
0.228CysGln: 0.228 ± 0.105
0.97CysArg: 0.97 ± 0.284
0.514CysSer: 0.514 ± 0.159
0.685CysThr: 0.685 ± 0.206
0.571CysVal: 0.571 ± 0.164
0.343CysTrp: 0.343 ± 0.125
0.4CysTyr: 0.4 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
6.223AspAla: 6.223 ± 0.539
1.028AspCys: 1.028 ± 0.253
4.396AspAsp: 4.396 ± 0.632
3.482AspGlu: 3.482 ± 0.497
1.484AspPhe: 1.484 ± 0.256
6.679AspGly: 6.679 ± 0.637
1.313AspHis: 1.313 ± 0.226
2.226AspIle: 2.226 ± 0.375
1.998AspLys: 1.998 ± 0.296
6.165AspLeu: 6.165 ± 0.492
1.142AspMet: 1.142 ± 0.251
1.656AspAsn: 1.656 ± 0.365
4.91AspPro: 4.91 ± 0.655
2.398AspGln: 2.398 ± 0.354
5.766AspArg: 5.766 ± 0.62
3.311AspSer: 3.311 ± 0.596
4.11AspThr: 4.11 ± 0.447
4.624AspVal: 4.624 ± 0.607
1.427AspTrp: 1.427 ± 0.268
1.827AspTyr: 1.827 ± 0.289
0.0AspXaa: 0.0 ± 0.0
Glu
6.108GluAla: 6.108 ± 0.698
1.142GluCys: 1.142 ± 0.288
3.083GluAsp: 3.083 ± 0.351
2.512GluGlu: 2.512 ± 0.504
2.226GluPhe: 2.226 ± 0.431
3.539GluGly: 3.539 ± 0.478
1.884GluHis: 1.884 ± 0.365
2.797GluIle: 2.797 ± 0.423
1.77GluLys: 1.77 ± 0.296
5.823GluLeu: 5.823 ± 0.662
1.484GluMet: 1.484 ± 0.317
2.055GluAsn: 2.055 ± 0.28
2.969GluPro: 2.969 ± 0.408
2.74GluGln: 2.74 ± 0.383
4.453GluArg: 4.453 ± 0.599
3.768GluSer: 3.768 ± 0.493
4.224GluThr: 4.224 ± 0.591
3.939GluVal: 3.939 ± 0.467
1.37GluTrp: 1.37 ± 0.264
1.884GluTyr: 1.884 ± 0.323
0.0GluXaa: 0.0 ± 0.0
Phe
2.911PheAla: 2.911 ± 0.446
0.4PheCys: 0.4 ± 0.144
2.455PheAsp: 2.455 ± 0.493
1.656PheGlu: 1.656 ± 0.321
0.913PhePhe: 0.913 ± 0.267
2.911PheGly: 2.911 ± 0.606
0.343PheHis: 0.343 ± 0.131
1.541PheIle: 1.541 ± 0.282
1.085PheLys: 1.085 ± 0.249
1.941PheLeu: 1.941 ± 0.329
0.685PheMet: 0.685 ± 0.2
1.142PheAsn: 1.142 ± 0.293
1.598PhePro: 1.598 ± 0.294
1.313PheGln: 1.313 ± 0.275
1.37PheArg: 1.37 ± 0.239
1.37PheSer: 1.37 ± 0.247
1.998PheThr: 1.998 ± 0.297
2.341PheVal: 2.341 ± 0.308
0.685PheTrp: 0.685 ± 0.153
1.028PheTyr: 1.028 ± 0.223
0.0PheXaa: 0.0 ± 0.0
Gly
9.705GlyAla: 9.705 ± 1.138
1.028GlyCys: 1.028 ± 0.255
5.652GlyAsp: 5.652 ± 0.498
4.167GlyGlu: 4.167 ± 0.531
2.626GlyPhe: 2.626 ± 0.457
10.732GlyGly: 10.732 ± 1.833
1.427GlyHis: 1.427 ± 0.218
4.053GlyIle: 4.053 ± 0.492
2.569GlyLys: 2.569 ± 0.384
6.165GlyLeu: 6.165 ± 0.643
2.283GlyMet: 2.283 ± 0.447
3.083GlyAsn: 3.083 ± 0.391
4.11GlyPro: 4.11 ± 0.721
2.283GlyGln: 2.283 ± 0.624
5.48GlyArg: 5.48 ± 0.6
5.766GlySer: 5.766 ± 0.778
6.337GlyThr: 6.337 ± 0.75
6.965GlyVal: 6.965 ± 0.607
2.226GlyTrp: 2.226 ± 0.357
1.884GlyTyr: 1.884 ± 0.335
0.0GlyXaa: 0.0 ± 0.0
His
1.656HisAla: 1.656 ± 0.288
0.457HisCys: 0.457 ± 0.195
1.199HisAsp: 1.199 ± 0.234
1.199HisGlu: 1.199 ± 0.239
0.571HisPhe: 0.571 ± 0.166
1.313HisGly: 1.313 ± 0.255
0.799HisHis: 0.799 ± 0.278
1.427HisIle: 1.427 ± 0.29
0.799HisLys: 0.799 ± 0.229
1.37HisLeu: 1.37 ± 0.247
0.4HisMet: 0.4 ± 0.132
0.913HisAsn: 0.913 ± 0.2
1.484HisPro: 1.484 ± 0.242
0.628HisGln: 0.628 ± 0.157
1.884HisArg: 1.884 ± 0.384
0.742HisSer: 0.742 ± 0.156
1.598HisThr: 1.598 ± 0.369
1.713HisVal: 1.713 ± 0.339
0.685HisTrp: 0.685 ± 0.231
0.856HisTyr: 0.856 ± 0.193
0.0HisXaa: 0.0 ± 0.0
Ile
5.48IleAla: 5.48 ± 0.423
0.742IleCys: 0.742 ± 0.242
3.768IleAsp: 3.768 ± 0.524
3.425IleGlu: 3.425 ± 0.321
0.742IlePhe: 0.742 ± 0.259
3.482IleGly: 3.482 ± 0.408
1.598IleHis: 1.598 ± 0.334
1.77IleIle: 1.77 ± 0.325
1.142IleLys: 1.142 ± 0.244
2.512IleLeu: 2.512 ± 0.46
0.571IleMet: 0.571 ± 0.17
2.398IleAsn: 2.398 ± 0.285
3.026IlePro: 3.026 ± 0.435
1.427IleGln: 1.427 ± 0.235
2.169IleArg: 2.169 ± 0.362
2.341IleSer: 2.341 ± 0.466
3.539IleThr: 3.539 ± 0.4
3.083IleVal: 3.083 ± 0.328
1.085IleTrp: 1.085 ± 0.267
0.856IleTyr: 0.856 ± 0.182
0.0IleXaa: 0.0 ± 0.0
Lys
4.224LysAla: 4.224 ± 0.569
0.628LysCys: 0.628 ± 0.201
1.541LysAsp: 1.541 ± 0.261
1.598LysGlu: 1.598 ± 0.309
1.199LysPhe: 1.199 ± 0.178
2.455LysGly: 2.455 ± 0.307
1.142LysHis: 1.142 ± 0.26
0.742LysIle: 0.742 ± 0.246
1.313LysLys: 1.313 ± 0.351
2.569LysLeu: 2.569 ± 0.451
0.799LysMet: 0.799 ± 0.203
0.913LysAsn: 0.913 ± 0.207
2.626LysPro: 2.626 ± 0.422
1.884LysGln: 1.884 ± 0.293
2.169LysArg: 2.169 ± 0.407
2.341LysSer: 2.341 ± 0.39
1.884LysThr: 1.884 ± 0.287
2.169LysVal: 2.169 ± 0.356
0.742LysTrp: 0.742 ± 0.245
0.685LysTyr: 0.685 ± 0.2
0.0LysXaa: 0.0 ± 0.0
Leu
8.163LeuAla: 8.163 ± 0.795
0.799LeuCys: 0.799 ± 0.243
4.852LeuAsp: 4.852 ± 0.576
4.053LeuGlu: 4.053 ± 0.523
2.797LeuPhe: 2.797 ± 0.297
4.967LeuGly: 4.967 ± 0.517
1.199LeuHis: 1.199 ± 0.245
3.197LeuIle: 3.197 ± 0.393
2.512LeuLys: 2.512 ± 0.398
5.252LeuLeu: 5.252 ± 0.562
1.598LeuMet: 1.598 ± 0.323
2.512LeuAsn: 2.512 ± 0.356
5.309LeuPro: 5.309 ± 0.743
3.083LeuGln: 3.083 ± 0.445
4.681LeuArg: 4.681 ± 0.699
4.396LeuSer: 4.396 ± 0.524
5.024LeuThr: 5.024 ± 0.496
4.51LeuVal: 4.51 ± 0.559
1.256LeuTrp: 1.256 ± 0.282
2.112LeuTyr: 2.112 ± 0.348
0.0LeuXaa: 0.0 ± 0.0
Met
2.112MetAla: 2.112 ± 0.384
0.0MetCys: 0.0 ± 0.0
1.199MetAsp: 1.199 ± 0.244
1.199MetGlu: 1.199 ± 0.231
0.571MetPhe: 0.571 ± 0.149
1.827MetGly: 1.827 ± 0.26
0.171MetHis: 0.171 ± 0.106
0.742MetIle: 0.742 ± 0.228
0.685MetLys: 0.685 ± 0.205
2.112MetLeu: 2.112 ± 0.28
0.685MetMet: 0.685 ± 0.21
0.97MetAsn: 0.97 ± 0.217
1.256MetPro: 1.256 ± 0.264
0.514MetGln: 0.514 ± 0.162
1.313MetArg: 1.313 ± 0.277
2.683MetSer: 2.683 ± 0.429
2.341MetThr: 2.341 ± 0.339
1.427MetVal: 1.427 ± 0.299
0.571MetTrp: 0.571 ± 0.166
0.457MetTyr: 0.457 ± 0.158
0.0MetXaa: 0.0 ± 0.0
Asn
3.597AsnAla: 3.597 ± 0.383
0.228AsnCys: 0.228 ± 0.086
1.941AsnAsp: 1.941 ± 0.274
1.77AsnGlu: 1.77 ± 0.388
0.97AsnPhe: 0.97 ± 0.278
4.282AsnGly: 4.282 ± 0.583
0.685AsnHis: 0.685 ± 0.155
1.941AsnIle: 1.941 ± 0.457
0.97AsnLys: 0.97 ± 0.258
2.283AsnLeu: 2.283 ± 0.373
0.571AsnMet: 0.571 ± 0.189
1.598AsnAsn: 1.598 ± 0.294
2.398AsnPro: 2.398 ± 0.302
1.199AsnGln: 1.199 ± 0.299
1.884AsnArg: 1.884 ± 0.376
1.713AsnSer: 1.713 ± 0.317
2.055AsnThr: 2.055 ± 0.298
2.398AsnVal: 2.398 ± 0.33
1.028AsnTrp: 1.028 ± 0.171
0.742AsnTyr: 0.742 ± 0.151
0.0AsnXaa: 0.0 ± 0.0
Pro
5.423ProAla: 5.423 ± 0.623
0.742ProCys: 0.742 ± 0.196
4.282ProAsp: 4.282 ± 0.437
4.795ProGlu: 4.795 ± 0.514
1.256ProPhe: 1.256 ± 0.293
6.165ProGly: 6.165 ± 0.746
1.427ProHis: 1.427 ± 0.263
2.112ProIle: 2.112 ± 0.259
2.112ProLys: 2.112 ± 0.388
4.053ProLeu: 4.053 ± 0.58
1.541ProMet: 1.541 ± 0.286
2.626ProAsn: 2.626 ± 0.365
4.224ProPro: 4.224 ± 0.568
1.827ProGln: 1.827 ± 0.372
3.368ProArg: 3.368 ± 0.529
3.482ProSer: 3.482 ± 0.479
3.311ProThr: 3.311 ± 0.464
4.738ProVal: 4.738 ± 0.534
1.142ProTrp: 1.142 ± 0.23
1.484ProTyr: 1.484 ± 0.259
0.0ProXaa: 0.0 ± 0.0
Gln
4.167GlnAla: 4.167 ± 0.577
0.343GlnCys: 0.343 ± 0.166
1.713GlnAsp: 1.713 ± 0.259
1.998GlnGlu: 1.998 ± 0.367
1.028GlnPhe: 1.028 ± 0.214
2.341GlnGly: 2.341 ± 0.558
0.856GlnHis: 0.856 ± 0.225
2.055GlnIle: 2.055 ± 0.323
1.313GlnLys: 1.313 ± 0.261
3.026GlnLeu: 3.026 ± 0.408
0.628GlnMet: 0.628 ± 0.197
1.085GlnAsn: 1.085 ± 0.319
2.512GlnPro: 2.512 ± 0.371
1.256GlnGln: 1.256 ± 0.236
2.398GlnArg: 2.398 ± 0.385
2.455GlnSer: 2.455 ± 0.415
1.713GlnThr: 1.713 ± 0.333
2.74GlnVal: 2.74 ± 0.432
0.571GlnTrp: 0.571 ± 0.217
0.742GlnTyr: 0.742 ± 0.259
0.0GlnXaa: 0.0 ± 0.0
Arg
6.679ArgAla: 6.679 ± 0.559
1.085ArgCys: 1.085 ± 0.292
4.339ArgAsp: 4.339 ± 0.562
5.138ArgGlu: 5.138 ± 0.683
2.226ArgPhe: 2.226 ± 0.406
3.939ArgGly: 3.939 ± 0.456
1.37ArgHis: 1.37 ± 0.232
3.768ArgIle: 3.768 ± 0.586
2.512ArgLys: 2.512 ± 0.396
4.738ArgLeu: 4.738 ± 0.552
2.398ArgMet: 2.398 ± 0.397
2.341ArgAsn: 2.341 ± 0.379
3.425ArgPro: 3.425 ± 0.469
1.541ArgGln: 1.541 ± 0.336
5.823ArgArg: 5.823 ± 0.821
3.482ArgSer: 3.482 ± 0.442
3.311ArgThr: 3.311 ± 0.504
5.652ArgVal: 5.652 ± 0.686
1.77ArgTrp: 1.77 ± 0.272
1.598ArgTyr: 1.598 ± 0.291
0.0ArgXaa: 0.0 ± 0.0
Ser
5.309SerAla: 5.309 ± 0.728
0.228SerCys: 0.228 ± 0.104
4.453SerAsp: 4.453 ± 0.637
3.083SerGlu: 3.083 ± 0.422
1.941SerPhe: 1.941 ± 0.313
6.337SerGly: 6.337 ± 0.876
1.085SerHis: 1.085 ± 0.255
3.083SerIle: 3.083 ± 0.438
2.226SerLys: 2.226 ± 0.352
3.482SerLeu: 3.482 ± 0.428
1.598SerMet: 1.598 ± 0.268
2.341SerAsn: 2.341 ± 0.43
3.711SerPro: 3.711 ± 0.381
1.713SerGln: 1.713 ± 0.285
3.083SerArg: 3.083 ± 0.418
4.053SerSer: 4.053 ± 0.623
3.368SerThr: 3.368 ± 0.456
4.339SerVal: 4.339 ± 0.587
1.37SerTrp: 1.37 ± 0.253
1.541SerTyr: 1.541 ± 0.239
0.0SerXaa: 0.0 ± 0.0
Thr
6.622ThrAla: 6.622 ± 0.556
0.799ThrCys: 0.799 ± 0.206
3.882ThrAsp: 3.882 ± 0.611
3.597ThrGlu: 3.597 ± 0.344
1.941ThrPhe: 1.941 ± 0.285
6.337ThrGly: 6.337 ± 0.606
1.598ThrHis: 1.598 ± 0.344
3.825ThrIle: 3.825 ± 0.483
1.541ThrLys: 1.541 ± 0.319
3.654ThrLeu: 3.654 ± 0.463
1.142ThrMet: 1.142 ± 0.247
2.398ThrAsn: 2.398 ± 0.481
3.711ThrPro: 3.711 ± 0.449
2.283ThrGln: 2.283 ± 0.312
3.996ThrArg: 3.996 ± 0.495
3.539ThrSer: 3.539 ± 0.437
5.195ThrThr: 5.195 ± 0.6
5.937ThrVal: 5.937 ± 0.522
1.427ThrTrp: 1.427 ± 0.374
1.713ThrTyr: 1.713 ± 0.247
0.0ThrXaa: 0.0 ± 0.0
Val
7.136ValAla: 7.136 ± 0.651
1.256ValCys: 1.256 ± 0.235
5.366ValAsp: 5.366 ± 0.582
4.795ValGlu: 4.795 ± 0.551
2.341ValPhe: 2.341 ± 0.307
6.508ValGly: 6.508 ± 0.793
1.541ValHis: 1.541 ± 0.32
2.74ValIle: 2.74 ± 0.373
2.398ValLys: 2.398 ± 0.33
5.024ValLeu: 5.024 ± 0.539
1.541ValMet: 1.541 ± 0.239
1.827ValAsn: 1.827 ± 0.356
4.339ValPro: 4.339 ± 0.466
3.14ValGln: 3.14 ± 0.347
4.738ValArg: 4.738 ± 0.522
4.795ValSer: 4.795 ± 0.52
5.195ValThr: 5.195 ± 0.531
6.508ValVal: 6.508 ± 0.603
2.112ValTrp: 2.112 ± 0.39
1.085ValTyr: 1.085 ± 0.248
0.0ValXaa: 0.0 ± 0.0
Trp
2.341TrpAla: 2.341 ± 0.325
0.343TrpCys: 0.343 ± 0.128
1.77TrpAsp: 1.77 ± 0.322
1.085TrpGlu: 1.085 ± 0.287
0.571TrpPhe: 0.571 ± 0.162
0.97TrpGly: 0.97 ± 0.244
0.628TrpHis: 0.628 ± 0.213
0.97TrpIle: 0.97 ± 0.205
0.799TrpLys: 0.799 ± 0.184
1.713TrpLeu: 1.713 ± 0.327
1.028TrpMet: 1.028 ± 0.254
0.742TrpAsn: 0.742 ± 0.226
1.427TrpPro: 1.427 ± 0.233
1.085TrpGln: 1.085 ± 0.274
1.941TrpArg: 1.941 ± 0.429
1.313TrpSer: 1.313 ± 0.323
1.713TrpThr: 1.713 ± 0.268
1.713TrpVal: 1.713 ± 0.418
0.97TrpTrp: 0.97 ± 0.198
0.4TrpTyr: 0.4 ± 0.128
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.455TyrAla: 2.455 ± 0.426
0.343TyrCys: 0.343 ± 0.13
1.941TyrAsp: 1.941 ± 0.374
1.941TyrGlu: 1.941 ± 0.298
0.856TyrPhe: 0.856 ± 0.167
2.169TyrGly: 2.169 ± 0.34
0.285TyrHis: 0.285 ± 0.114
1.37TyrIle: 1.37 ± 0.225
0.685TyrLys: 0.685 ± 0.195
1.827TyrLeu: 1.827 ± 0.289
0.228TyrMet: 0.228 ± 0.111
0.742TyrAsn: 0.742 ± 0.205
1.085TyrPro: 1.085 ± 0.223
0.799TyrGln: 0.799 ± 0.199
1.598TyrArg: 1.598 ± 0.332
0.97TyrSer: 0.97 ± 0.241
1.77TyrThr: 1.77 ± 0.381
2.055TyrVal: 2.055 ± 0.32
0.628TyrTrp: 0.628 ± 0.176
0.685TyrTyr: 0.685 ± 0.213
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 98 proteins (17518 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski