Streptococcus phage phi-SsuSSJ28_rum

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3MLI3|A0A7G3MLI3_9CAUD Terminase small subunit OS=Streptococcus phage phi-SsuSSJ28_rum OX=2664406 PE=4 SV=1
MM1 pKa = 6.71WTDD4 pKa = 3.72GRR6 pKa = 11.84IDD8 pKa = 3.66YY9 pKa = 10.43QGQKK13 pKa = 9.41VDD15 pKa = 4.19YY16 pKa = 8.62IAKK19 pKa = 9.96VSPQPSEE26 pKa = 3.82VGIDD30 pKa = 3.55LGCVFKK36 pKa = 11.22LDD38 pKa = 3.42IEE40 pKa = 4.55VAEE43 pKa = 4.24EE44 pKa = 4.3TIVSYY49 pKa = 11.22DD50 pKa = 3.91RR51 pKa = 11.84GWEE54 pKa = 3.93IYY56 pKa = 10.15PEE58 pKa = 4.25TEE60 pKa = 3.48NRR62 pKa = 11.84EE63 pKa = 4.4AILEE67 pKa = 4.11AVLLVLTVV75 pKa = 3.22

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3MP28|A0A7G3MP28_9CAUD Uncharacterized protein OS=Streptococcus phage phi-SsuSSJ28_rum OX=2664406 PE=4 SV=1
MM1 pKa = 7.84SRR3 pKa = 11.84FCKK6 pKa = 10.05FGKK9 pKa = 10.08LHH11 pKa = 4.59VTKK14 pKa = 10.99GNVDD18 pKa = 3.39KK19 pKa = 11.29LLGILLTASKK29 pKa = 10.2EE30 pKa = 4.37LKK32 pKa = 10.25RR33 pKa = 11.84SLAPTGNLYY42 pKa = 10.5RR43 pKa = 5.58

Molecular weight: 4.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

18277

33

1515

268.8

30.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.38 ± 0.352

1.007 ± 0.155

5.854 ± 0.253

7.447 ± 0.344

3.989 ± 0.207

6.412 ± 0.273

2.112 ± 0.153

7.08 ± 0.278

7.49 ± 0.261

8.875 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.413 ± 0.186

5.083 ± 0.18

2.998 ± 0.211

4.202 ± 0.22

4.487 ± 0.213

6.287 ± 0.331

6.237 ± 0.426

6.33 ± 0.228

1.351 ± 0.091

3.967 ± 0.305
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski