Amino acid dipepetide frequency for Streptococcus phage phi-SsuSSJ28_rum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.174AlaAla: 3.174 ± 0.933
0.602AlaCys: 0.602 ± 0.203
3.885AlaAsp: 3.885 ± 0.419
4.487AlaGlu: 4.487 ± 0.521
2.572AlaPhe: 2.572 ± 0.32
4.377AlaGly: 4.377 ± 0.615
0.657AlaHis: 0.657 ± 0.21
5.526AlaIle: 5.526 ± 0.608
5.198AlaLys: 5.198 ± 0.516
5.362AlaLeu: 5.362 ± 0.815
1.696AlaMet: 1.696 ± 0.29
3.338AlaAsn: 3.338 ± 0.382
1.587AlaPro: 1.587 ± 0.289
2.626AlaGln: 2.626 ± 0.658
2.572AlaArg: 2.572 ± 0.339
4.377AlaSer: 4.377 ± 0.558
4.049AlaThr: 4.049 ± 0.539
4.158AlaVal: 4.158 ± 0.454
0.711AlaTrp: 0.711 ± 0.205
2.845AlaTyr: 2.845 ± 0.413
0.0AlaXaa: 0.0 ± 0.0
Cys
0.711CysAla: 0.711 ± 0.192
0.328CysCys: 0.328 ± 0.105
0.438CysAsp: 0.438 ± 0.144
0.602CysGlu: 0.602 ± 0.177
0.219CysPhe: 0.219 ± 0.106
0.766CysGly: 0.766 ± 0.136
0.383CysHis: 0.383 ± 0.141
0.602CysIle: 0.602 ± 0.191
0.711CysLys: 0.711 ± 0.259
0.711CysLeu: 0.711 ± 0.264
0.109CysMet: 0.109 ± 0.082
0.547CysAsn: 0.547 ± 0.173
0.383CysPro: 0.383 ± 0.144
0.93CysGln: 0.93 ± 0.249
0.547CysArg: 0.547 ± 0.21
0.492CysSer: 0.492 ± 0.205
0.328CysThr: 0.328 ± 0.144
0.657CysVal: 0.657 ± 0.168
0.0CysTrp: 0.0 ± 0.0
0.602CysTyr: 0.602 ± 0.206
0.0CysXaa: 0.0 ± 0.0
Asp
3.119AspAla: 3.119 ± 0.419
0.602AspCys: 0.602 ± 0.257
3.064AspAsp: 3.064 ± 0.503
5.089AspGlu: 5.089 ± 0.657
3.174AspPhe: 3.174 ± 0.425
4.541AspGly: 4.541 ± 0.452
1.258AspHis: 1.258 ± 0.327
4.706AspIle: 4.706 ± 0.514
3.94AspLys: 3.94 ± 0.373
4.924AspLeu: 4.924 ± 0.635
2.079AspMet: 2.079 ± 0.385
3.228AspAsn: 3.228 ± 0.498
1.532AspPro: 1.532 ± 0.341
1.423AspGln: 1.423 ± 0.237
2.353AspArg: 2.353 ± 0.508
3.557AspSer: 3.557 ± 0.506
2.791AspThr: 2.791 ± 0.409
3.283AspVal: 3.283 ± 0.474
0.875AspTrp: 0.875 ± 0.19
3.009AspTyr: 3.009 ± 0.511
0.0AspXaa: 0.0 ± 0.0
Glu
4.651GluAla: 4.651 ± 0.425
0.766GluCys: 0.766 ± 0.214
4.158GluAsp: 4.158 ± 0.564
6.019GluGlu: 6.019 ± 0.794
2.681GluPhe: 2.681 ± 0.488
4.596GluGly: 4.596 ± 0.407
1.587GluHis: 1.587 ± 0.343
5.253GluIle: 5.253 ± 0.557
6.949GluLys: 6.949 ± 0.898
7.387GluLeu: 7.387 ± 0.741
2.462GluMet: 2.462 ± 0.455
4.158GluAsn: 4.158 ± 0.586
1.587GluPro: 1.587 ± 0.407
3.885GluGln: 3.885 ± 0.37
2.626GluArg: 2.626 ± 0.435
3.338GluSer: 3.338 ± 0.361
4.487GluThr: 4.487 ± 0.509
4.377GluVal: 4.377 ± 0.46
0.766GluTrp: 0.766 ± 0.183
2.681GluTyr: 2.681 ± 0.517
0.0GluXaa: 0.0 ± 0.0
Phe
2.408PheAla: 2.408 ± 0.402
0.657PheCys: 0.657 ± 0.181
2.791PheAsp: 2.791 ± 0.441
3.228PheGlu: 3.228 ± 0.417
1.532PhePhe: 1.532 ± 0.32
2.298PheGly: 2.298 ± 0.351
1.149PheHis: 1.149 ± 0.25
2.189PheIle: 2.189 ± 0.364
3.283PheLys: 3.283 ± 0.494
3.174PheLeu: 3.174 ± 0.471
0.875PheMet: 0.875 ± 0.247
2.517PheAsn: 2.517 ± 0.408
1.094PhePro: 1.094 ± 0.287
1.313PheGln: 1.313 ± 0.272
1.806PheArg: 1.806 ± 0.32
2.353PheSer: 2.353 ± 0.359
2.681PheThr: 2.681 ± 0.426
1.86PheVal: 1.86 ± 0.403
0.602PheTrp: 0.602 ± 0.185
2.079PheTyr: 2.079 ± 0.327
0.0PheXaa: 0.0 ± 0.0
Gly
3.666GlyAla: 3.666 ± 0.493
0.492GlyCys: 0.492 ± 0.145
4.268GlyAsp: 4.268 ± 0.679
4.104GlyGlu: 4.104 ± 0.47
2.517GlyPhe: 2.517 ± 0.325
3.447GlyGly: 3.447 ± 0.5
2.243GlyHis: 2.243 ± 0.529
6.019GlyIle: 6.019 ± 0.692
4.596GlyLys: 4.596 ± 0.462
5.8GlyLeu: 5.8 ± 0.569
1.915GlyMet: 1.915 ± 0.315
3.611GlyAsn: 3.611 ± 0.502
0.93GlyPro: 0.93 ± 0.257
2.626GlyGln: 2.626 ± 0.424
3.228GlyArg: 3.228 ± 0.419
3.338GlySer: 3.338 ± 0.478
3.611GlyThr: 3.611 ± 0.475
3.83GlyVal: 3.83 ± 0.436
0.875GlyTrp: 0.875 ± 0.191
3.009GlyTyr: 3.009 ± 0.423
0.0GlyXaa: 0.0 ± 0.0
His
0.985HisAla: 0.985 ± 0.208
0.274HisCys: 0.274 ± 0.122
1.313HisAsp: 1.313 ± 0.332
1.094HisGlu: 1.094 ± 0.238
1.204HisPhe: 1.204 ± 0.239
1.86HisGly: 1.86 ± 0.287
0.985HisHis: 0.985 ± 0.354
1.368HisIle: 1.368 ± 0.266
0.875HisLys: 0.875 ± 0.214
1.86HisLeu: 1.86 ± 0.326
0.383HisMet: 0.383 ± 0.15
0.985HisAsn: 0.985 ± 0.256
1.204HisPro: 1.204 ± 0.282
1.04HisGln: 1.04 ± 0.306
1.204HisArg: 1.204 ± 0.267
0.875HisSer: 0.875 ± 0.317
1.094HisThr: 1.094 ± 0.255
1.313HisVal: 1.313 ± 0.262
0.274HisTrp: 0.274 ± 0.122
0.93HisTyr: 0.93 ± 0.268
0.0HisXaa: 0.0 ± 0.0
Ile
5.253IleAla: 5.253 ± 0.511
0.657IleCys: 0.657 ± 0.185
5.472IleAsp: 5.472 ± 0.591
4.979IleGlu: 4.979 ± 0.577
2.517IlePhe: 2.517 ± 0.458
4.76IleGly: 4.76 ± 0.575
0.985IleHis: 0.985 ± 0.227
4.432IleIle: 4.432 ± 0.516
5.089IleLys: 5.089 ± 0.622
6.074IleLeu: 6.074 ± 0.549
1.204IleMet: 1.204 ± 0.329
3.174IleAsn: 3.174 ± 0.405
2.462IlePro: 2.462 ± 0.284
2.462IleGln: 2.462 ± 0.329
2.791IleArg: 2.791 ± 0.315
5.089IleSer: 5.089 ± 0.707
4.377IleThr: 4.377 ± 0.675
4.76IleVal: 4.76 ± 0.53
1.258IleTrp: 1.258 ± 0.288
3.009IleTyr: 3.009 ± 0.509
0.0IleXaa: 0.0 ± 0.0
Lys
5.691LysAla: 5.691 ± 0.52
0.438LysCys: 0.438 ± 0.145
3.83LysAsp: 3.83 ± 0.476
5.745LysGlu: 5.745 ± 0.47
2.681LysPhe: 2.681 ± 0.429
4.979LysGly: 4.979 ± 0.508
1.696LysHis: 1.696 ± 0.301
5.034LysIle: 5.034 ± 0.522
6.074LysLys: 6.074 ± 0.663
6.511LysLeu: 6.511 ± 0.693
2.189LysMet: 2.189 ± 0.35
3.174LysAsn: 3.174 ± 0.443
2.243LysPro: 2.243 ± 0.319
3.392LysGln: 3.392 ± 0.485
3.885LysArg: 3.885 ± 0.58
4.158LysSer: 4.158 ± 0.478
3.885LysThr: 3.885 ± 0.475
5.253LysVal: 5.253 ± 0.5
0.985LysTrp: 0.985 ± 0.214
3.064LysTyr: 3.064 ± 0.462
0.0LysXaa: 0.0 ± 0.0
Leu
5.964LeuAla: 5.964 ± 0.585
0.711LeuCys: 0.711 ± 0.217
5.198LeuAsp: 5.198 ± 0.517
7.004LeuGlu: 7.004 ± 0.799
3.119LeuPhe: 3.119 ± 0.473
5.8LeuGly: 5.8 ± 0.581
1.587LeuHis: 1.587 ± 0.283
4.979LeuIle: 4.979 ± 0.571
7.223LeuLys: 7.223 ± 0.684
8.043LeuLeu: 8.043 ± 0.832
1.751LeuMet: 1.751 ± 0.237
4.104LeuAsn: 4.104 ± 0.437
2.955LeuPro: 2.955 ± 0.392
3.174LeuGln: 3.174 ± 0.472
3.885LeuArg: 3.885 ± 0.548
6.894LeuSer: 6.894 ± 0.49
6.402LeuThr: 6.402 ± 0.552
5.526LeuVal: 5.526 ± 0.68
0.766LeuTrp: 0.766 ± 0.161
3.666LeuTyr: 3.666 ± 0.588
0.0LeuXaa: 0.0 ± 0.0
Met
1.806MetAla: 1.806 ± 0.277
0.109MetCys: 0.109 ± 0.078
2.025MetAsp: 2.025 ± 0.386
1.587MetGlu: 1.587 ± 0.288
0.766MetPhe: 0.766 ± 0.202
1.423MetGly: 1.423 ± 0.315
0.328MetHis: 0.328 ± 0.121
1.696MetIle: 1.696 ± 0.299
1.587MetLys: 1.587 ± 0.299
2.079MetLeu: 2.079 ± 0.348
1.204MetMet: 1.204 ± 0.332
0.875MetAsn: 0.875 ± 0.205
0.547MetPro: 0.547 ± 0.207
0.766MetGln: 0.766 ± 0.199
1.313MetArg: 1.313 ± 0.259
1.97MetSer: 1.97 ± 0.379
1.915MetThr: 1.915 ± 0.347
1.423MetVal: 1.423 ± 0.268
0.164MetTrp: 0.164 ± 0.109
0.547MetTyr: 0.547 ± 0.15
0.0MetXaa: 0.0 ± 0.0
Asn
3.666AsnAla: 3.666 ± 0.606
0.274AsnCys: 0.274 ± 0.139
2.845AsnAsp: 2.845 ± 0.39
3.502AsnGlu: 3.502 ± 0.53
2.079AsnPhe: 2.079 ± 0.328
4.323AsnGly: 4.323 ± 0.544
1.258AsnHis: 1.258 ± 0.247
3.557AsnIle: 3.557 ± 0.4
3.228AsnLys: 3.228 ± 0.41
4.76AsnLeu: 4.76 ± 0.578
0.875AsnMet: 0.875 ± 0.192
2.517AsnAsn: 2.517 ± 0.395
1.97AsnPro: 1.97 ± 0.323
2.9AsnGln: 2.9 ± 0.42
2.462AsnArg: 2.462 ± 0.431
3.174AsnSer: 3.174 ± 0.421
2.298AsnThr: 2.298 ± 0.38
2.681AsnVal: 2.681 ± 0.434
0.93AsnTrp: 0.93 ± 0.251
1.532AsnTyr: 1.532 ± 0.384
0.0AsnXaa: 0.0 ± 0.0
Pro
1.423ProAla: 1.423 ± 0.294
0.383ProCys: 0.383 ± 0.148
1.532ProAsp: 1.532 ± 0.287
2.626ProGlu: 2.626 ± 0.42
1.368ProPhe: 1.368 ± 0.304
1.04ProGly: 1.04 ± 0.311
0.766ProHis: 0.766 ± 0.186
1.86ProIle: 1.86 ± 0.242
2.572ProLys: 2.572 ± 0.476
2.408ProLeu: 2.408 ± 0.403
0.492ProMet: 0.492 ± 0.137
1.86ProAsn: 1.86 ± 0.374
0.821ProPro: 0.821 ± 0.24
0.985ProGln: 0.985 ± 0.253
1.368ProArg: 1.368 ± 0.276
2.408ProSer: 2.408 ± 0.331
2.134ProThr: 2.134 ± 0.292
2.243ProVal: 2.243 ± 0.387
0.274ProTrp: 0.274 ± 0.116
1.423ProTyr: 1.423 ± 0.319
0.0ProXaa: 0.0 ± 0.0
Gln
3.83GlnAla: 3.83 ± 0.47
0.602GlnCys: 0.602 ± 0.214
2.134GlnAsp: 2.134 ± 0.344
2.736GlnGlu: 2.736 ± 0.303
1.97GlnPhe: 1.97 ± 0.312
2.243GlnGly: 2.243 ± 0.385
0.383GlnHis: 0.383 ± 0.129
2.736GlnIle: 2.736 ± 0.409
2.626GlnLys: 2.626 ± 0.426
3.94GlnLeu: 3.94 ± 0.403
1.149GlnMet: 1.149 ± 0.279
2.517GlnAsn: 2.517 ± 0.389
1.368GlnPro: 1.368 ± 0.276
1.806GlnGln: 1.806 ± 0.279
1.477GlnArg: 1.477 ± 0.347
2.134GlnSer: 2.134 ± 0.45
2.736GlnThr: 2.736 ± 0.648
4.104GlnVal: 4.104 ± 0.482
0.711GlnTrp: 0.711 ± 0.195
0.821GlnTyr: 0.821 ± 0.256
0.0GlnXaa: 0.0 ± 0.0
Arg
1.915ArgAla: 1.915 ± 0.329
0.657ArgCys: 0.657 ± 0.167
2.517ArgAsp: 2.517 ± 0.391
3.174ArgGlu: 3.174 ± 0.431
1.587ArgPhe: 1.587 ± 0.334
2.517ArgGly: 2.517 ± 0.351
0.602ArgHis: 0.602 ± 0.186
3.228ArgIle: 3.228 ± 0.433
3.94ArgLys: 3.94 ± 0.571
4.596ArgLeu: 4.596 ± 0.514
0.985ArgMet: 0.985 ± 0.247
2.353ArgAsn: 2.353 ± 0.296
1.641ArgPro: 1.641 ± 0.372
2.189ArgGln: 2.189 ± 0.347
1.97ArgArg: 1.97 ± 0.393
2.681ArgSer: 2.681 ± 0.32
2.791ArgThr: 2.791 ± 0.549
3.064ArgVal: 3.064 ± 0.427
0.821ArgTrp: 0.821 ± 0.213
1.641ArgTyr: 1.641 ± 0.325
0.0ArgXaa: 0.0 ± 0.0
Ser
3.83SerAla: 3.83 ± 0.579
0.711SerCys: 0.711 ± 0.192
3.338SerAsp: 3.338 ± 0.522
4.104SerGlu: 4.104 ± 0.532
3.447SerPhe: 3.447 ± 0.579
4.377SerGly: 4.377 ± 0.512
1.641SerHis: 1.641 ± 0.262
4.651SerIle: 4.651 ± 0.547
4.268SerLys: 4.268 ± 0.484
5.253SerLeu: 5.253 ± 0.606
1.368SerMet: 1.368 ± 0.214
2.681SerAsn: 2.681 ± 0.509
1.915SerPro: 1.915 ± 0.29
2.845SerGln: 2.845 ± 0.587
2.9SerArg: 2.9 ± 0.336
4.213SerSer: 4.213 ± 0.699
4.323SerThr: 4.323 ± 0.514
3.775SerVal: 3.775 ± 0.4
0.875SerTrp: 0.875 ± 0.187
2.353SerTyr: 2.353 ± 0.39
0.0SerXaa: 0.0 ± 0.0
Thr
4.377ThrAla: 4.377 ± 0.487
0.109ThrCys: 0.109 ± 0.082
3.009ThrAsp: 3.009 ± 0.459
4.596ThrGlu: 4.596 ± 0.559
2.243ThrPhe: 2.243 ± 0.439
4.213ThrGly: 4.213 ± 0.525
0.821ThrHis: 0.821 ± 0.228
4.924ThrIle: 4.924 ± 0.692
4.377ThrLys: 4.377 ± 0.477
5.417ThrLeu: 5.417 ± 0.55
0.985ThrMet: 0.985 ± 0.22
2.845ThrAsn: 2.845 ± 0.459
1.806ThrPro: 1.806 ± 0.413
2.572ThrGln: 2.572 ± 0.667
2.517ThrArg: 2.517 ± 0.412
4.541ThrSer: 4.541 ± 0.956
4.815ThrThr: 4.815 ± 0.652
4.979ThrVal: 4.979 ± 0.538
0.875ThrTrp: 0.875 ± 0.24
2.353ThrTyr: 2.353 ± 0.382
0.0ThrXaa: 0.0 ± 0.0
Val
4.158ValAla: 4.158 ± 0.55
0.711ValCys: 0.711 ± 0.238
3.721ValAsp: 3.721 ± 0.536
5.417ValGlu: 5.417 ± 0.802
2.243ValPhe: 2.243 ± 0.411
3.338ValGly: 3.338 ± 0.481
1.313ValHis: 1.313 ± 0.262
4.377ValIle: 4.377 ± 0.531
4.651ValLys: 4.651 ± 0.447
5.909ValLeu: 5.909 ± 0.491
1.368ValMet: 1.368 ± 0.26
3.174ValAsn: 3.174 ± 0.514
2.298ValPro: 2.298 ± 0.295
2.462ValGln: 2.462 ± 0.371
3.174ValArg: 3.174 ± 0.47
4.049ValSer: 4.049 ± 0.627
4.541ValThr: 4.541 ± 0.526
3.338ValVal: 3.338 ± 0.435
1.04ValTrp: 1.04 ± 0.264
2.025ValTyr: 2.025 ± 0.338
0.0ValXaa: 0.0 ± 0.0
Trp
0.985TrpAla: 0.985 ± 0.21
0.219TrpCys: 0.219 ± 0.108
0.383TrpAsp: 0.383 ± 0.151
1.368TrpGlu: 1.368 ± 0.266
0.657TrpPhe: 0.657 ± 0.19
0.711TrpGly: 0.711 ± 0.161
0.383TrpHis: 0.383 ± 0.138
0.821TrpIle: 0.821 ± 0.187
0.93TrpLys: 0.93 ± 0.262
0.985TrpLeu: 0.985 ± 0.188
0.383TrpMet: 0.383 ± 0.117
1.258TrpAsn: 1.258 ± 0.327
0.055TrpPro: 0.055 ± 0.056
0.711TrpGln: 0.711 ± 0.211
0.492TrpArg: 0.492 ± 0.191
0.766TrpSer: 0.766 ± 0.318
0.875TrpThr: 0.875 ± 0.252
0.875TrpVal: 0.875 ± 0.208
0.274TrpTrp: 0.274 ± 0.12
0.383TrpTyr: 0.383 ± 0.137
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.189TyrAla: 2.189 ± 0.331
0.766TyrCys: 0.766 ± 0.215
2.626TyrAsp: 2.626 ± 0.446
3.009TyrGlu: 3.009 ± 0.459
1.313TyrPhe: 1.313 ± 0.322
2.517TyrGly: 2.517 ± 0.392
1.094TyrHis: 1.094 ± 0.311
2.845TyrIle: 2.845 ± 0.481
2.791TyrLys: 2.791 ± 0.485
3.557TyrLeu: 3.557 ± 0.459
0.602TyrMet: 0.602 ± 0.191
1.915TyrAsn: 1.915 ± 0.349
1.587TyrPro: 1.587 ± 0.25
1.915TyrGln: 1.915 ± 0.294
2.298TyrArg: 2.298 ± 0.383
2.462TyrSer: 2.462 ± 0.347
2.243TyrThr: 2.243 ± 0.489
1.806TyrVal: 1.806 ± 0.33
0.438TyrTrp: 0.438 ± 0.151
1.696TyrTyr: 1.696 ± 0.409
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (18277 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski